Experiment: Motility assay, center cut sample 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliL are separated by 38 nucleotides fliL and Sama_2292 are separated by 85 nucleotides
Sama_2290: fliM - flagellar motor switch protein FliM (RefSeq), at 2,754,749 to 2,755,777
fliM
Sama_2291: fliL - flagellar basal body-associated protein FliL (RefSeq), at 2,755,816 to 2,756,343
fliL
Sama_2292: Sama_2292 - flagellar hook-length control protein FliK (RefSeq), at 2,756,429 to 2,758,210
_2292
Position (kb)
2755
2756
2757 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2754.840 kb on - strand at 2754.886 kb on - strand, within fliM at 2754.918 kb on + strand, within fliM at 2754.918 kb on + strand, within fliM at 2754.919 kb on - strand, within fliM at 2754.981 kb on - strand, within fliM at 2755.040 kb on - strand, within fliM at 2755.040 kb on - strand, within fliM at 2755.076 kb on - strand, within fliM at 2755.129 kb on - strand, within fliM at 2755.196 kb on + strand, within fliM at 2755.205 kb on - strand, within fliM at 2755.291 kb on + strand, within fliM at 2755.292 kb on - strand, within fliM at 2755.294 kb on - strand, within fliM at 2755.414 kb on + strand, within fliM at 2755.414 kb on + strand, within fliM at 2755.442 kb on - strand, within fliM at 2755.477 kb on + strand, within fliM at 2755.505 kb on - strand, within fliM at 2755.512 kb on + strand, within fliM at 2755.513 kb on - strand, within fliM at 2755.513 kb on - strand, within fliM at 2755.513 kb on - strand, within fliM at 2755.544 kb on - strand, within fliM at 2755.573 kb on + strand, within fliM at 2755.586 kb on + strand, within fliM at 2755.587 kb on - strand, within fliM at 2755.587 kb on - strand, within fliM at 2755.587 kb on - strand, within fliM at 2755.587 kb on - strand, within fliM at 2755.762 kb on + strand at 2755.766 kb on + strand at 2755.766 kb on + strand at 2755.766 kb on - strand at 2755.800 kb on - strand at 2755.805 kb on + strand at 2755.806 kb on - strand at 2755.806 kb on - strand at 2755.806 kb on - strand at 2755.818 kb on - strand at 2755.818 kb on - strand at 2755.885 kb on - strand, within fliL at 2755.885 kb on - strand, within fliL at 2755.885 kb on - strand, within fliL at 2755.888 kb on - strand, within fliL at 2755.892 kb on - strand, within fliL at 2755.892 kb on - strand, within fliL at 2755.892 kb on - strand, within fliL at 2755.916 kb on - strand, within fliL at 2755.916 kb on - strand, within fliL at 2755.916 kb on - strand, within fliL at 2756.014 kb on - strand, within fliL at 2756.014 kb on - strand, within fliL at 2756.124 kb on - strand, within fliL at 2756.124 kb on - strand, within fliL at 2756.124 kb on - strand, within fliL at 2756.249 kb on - strand, within fliL at 2756.293 kb on - strand at 2756.293 kb on - strand at 2756.293 kb on - strand at 2756.293 kb on - strand at 2756.311 kb on - strand at 2756.324 kb on - strand at 2756.324 kb on - strand at 2756.404 kb on - strand at 2756.404 kb on - strand at 2756.404 kb on - strand at 2756.404 kb on - strand at 2756.416 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.423 kb on - strand at 2756.423 kb on - strand at 2756.431 kb on - strand at 2756.437 kb on - strand at 2756.437 kb on - strand at 2756.437 kb on - strand at 2756.437 kb on - strand at 2756.440 kb on - strand at 2756.440 kb on - strand at 2756.440 kb on - strand at 2756.445 kb on - strand at 2756.445 kb on - strand at 2756.445 kb on - strand at 2756.580 kb on - strand at 2756.652 kb on - strand, within Sama_2292 at 2756.652 kb on - strand, within Sama_2292 at 2756.708 kb on + strand, within Sama_2292 at 2756.771 kb on + strand, within Sama_2292 at 2756.771 kb on + strand, within Sama_2292 at 2756.772 kb on - strand, within Sama_2292 at 2756.794 kb on + strand, within Sama_2292 at 2756.795 kb on - strand, within Sama_2292 at 2756.803 kb on + strand, within Sama_2292 at 2756.804 kb on - strand, within Sama_2292 at 2756.867 kb on - strand, within Sama_2292 at 2756.933 kb on - strand, within Sama_2292 at 2756.951 kb on + strand, within Sama_2292 at 2756.951 kb on + strand, within Sama_2292 at 2756.952 kb on - strand, within Sama_2292 at 2756.975 kb on + strand, within Sama_2292 at 2756.975 kb on + strand, within Sama_2292 at 2756.975 kb on + strand, within Sama_2292 at 2756.975 kb on + strand, within Sama_2292 at 2756.975 kb on + strand, within Sama_2292 at 2756.976 kb on - strand, within Sama_2292 at 2756.976 kb on - strand, within Sama_2292 at 2756.976 kb on - strand, within Sama_2292 at 2756.976 kb on - strand, within Sama_2292 at 2756.995 kb on + strand, within Sama_2292 at 2757.114 kb on - strand, within Sama_2292 at 2757.160 kb on + strand, within Sama_2292 at 2757.160 kb on + strand, within Sama_2292 at 2757.215 kb on - strand, within Sama_2292 at 2757.317 kb on - strand, within Sama_2292 at 2757.329 kb on + strand, within Sama_2292 at 2757.330 kb on - strand, within Sama_2292 at 2757.330 kb on - strand, within Sama_2292
Per-strain Table
Position Strand Gene LocusTag Fraction Motility assay, center cut sample 2 remove 2,754,840 - +0.1 2,754,886 - fliM Sama_2290 0.13 -3.2 2,754,918 + fliM Sama_2290 0.16 -0.4 2,754,918 + fliM Sama_2290 0.16 -2.6 2,754,919 - fliM Sama_2290 0.17 -2.1 2,754,981 - fliM Sama_2290 0.23 -2.6 2,755,040 - fliM Sama_2290 0.28 +0.6 2,755,040 - fliM Sama_2290 0.28 -2.1 2,755,076 - fliM Sama_2290 0.32 -1.9 2,755,129 - fliM Sama_2290 0.37 -0.9 2,755,196 + fliM Sama_2290 0.43 -1.2 2,755,205 - fliM Sama_2290 0.44 -2.8 2,755,291 + fliM Sama_2290 0.53 -1.9 2,755,292 - fliM Sama_2290 0.53 -2.2 2,755,294 - fliM Sama_2290 0.53 -0.7 2,755,414 + fliM Sama_2290 0.65 -0.9 2,755,414 + fliM Sama_2290 0.65 -1.2 2,755,442 - fliM Sama_2290 0.67 +0.1 2,755,477 + fliM Sama_2290 0.71 -1.7 2,755,505 - fliM Sama_2290 0.73 -0.7 2,755,512 + fliM Sama_2290 0.74 -2.4 2,755,513 - fliM Sama_2290 0.74 -1.8 2,755,513 - fliM Sama_2290 0.74 -2.3 2,755,513 - fliM Sama_2290 0.74 -3.4 2,755,544 - fliM Sama_2290 0.77 -0.7 2,755,573 + fliM Sama_2290 0.80 -1.7 2,755,586 + fliM Sama_2290 0.81 -2.1 2,755,587 - fliM Sama_2290 0.81 -1.8 2,755,587 - fliM Sama_2290 0.81 -3.2 2,755,587 - fliM Sama_2290 0.81 -2.1 2,755,587 - fliM Sama_2290 0.81 -1.2 2,755,762 + -3.5 2,755,766 + -0.4 2,755,766 + -0.7 2,755,766 - -1.8 2,755,800 - +2.1 2,755,805 + -3.4 2,755,806 - +0.8 2,755,806 - +0.6 2,755,806 - +2.2 2,755,818 - -0.7 2,755,818 - +1.4 2,755,885 - fliL Sama_2291 0.13 -2.2 2,755,885 - fliL Sama_2291 0.13 -0.7 2,755,885 - fliL Sama_2291 0.13 -2.4 2,755,888 - fliL Sama_2291 0.14 -1.8 2,755,892 - fliL Sama_2291 0.14 -2.4 2,755,892 - fliL Sama_2291 0.14 -2.1 2,755,892 - fliL Sama_2291 0.14 -3.1 2,755,916 - fliL Sama_2291 0.19 +0.6 2,755,916 - fliL Sama_2291 0.19 -2.2 2,755,916 - fliL Sama_2291 0.19 -1.9 2,756,014 - fliL Sama_2291 0.38 -0.9 2,756,014 - fliL Sama_2291 0.38 -1.4 2,756,124 - fliL Sama_2291 0.58 -3.2 2,756,124 - fliL Sama_2291 0.58 -1.5 2,756,124 - fliL Sama_2291 0.58 -0.7 2,756,249 - fliL Sama_2291 0.82 -0.4 2,756,293 - -2.5 2,756,293 - +0.1 2,756,293 - +0.8 2,756,293 - +0.1 2,756,311 - -1.4 2,756,324 - -2.0 2,756,324 - -3.0 2,756,404 - +1.0 2,756,404 - -0.1 2,756,404 - -0.9 2,756,404 - -2.4 2,756,416 - +1.3 2,756,418 - +3.2 2,756,418 - +0.7 2,756,418 - -0.9 2,756,418 - +0.5 2,756,418 - +0.6 2,756,418 - -2.5 2,756,423 - +1.3 2,756,423 - -1.2 2,756,431 - -0.5 2,756,437 - -3.2 2,756,437 - -0.7 2,756,437 - -3.9 2,756,437 - -1.9 2,756,440 - -2.6 2,756,440 - -2.1 2,756,440 - -2.2 2,756,445 - -3.3 2,756,445 - -2.1 2,756,445 - -2.4 2,756,580 - -1.9 2,756,652 - Sama_2292 0.13 +0.6 2,756,652 - Sama_2292 0.13 -0.9 2,756,708 + Sama_2292 0.16 -1.4 2,756,771 + Sama_2292 0.19 -2.7 2,756,771 + Sama_2292 0.19 -3.9 2,756,772 - Sama_2292 0.19 -1.7 2,756,794 + Sama_2292 0.20 -2.1 2,756,795 - Sama_2292 0.21 -2.1 2,756,803 + Sama_2292 0.21 -0.9 2,756,804 - Sama_2292 0.21 -1.2 2,756,867 - Sama_2292 0.25 -1.5 2,756,933 - Sama_2292 0.28 -2.1 2,756,951 + Sama_2292 0.29 -2.6 2,756,951 + Sama_2292 0.29 -1.4 2,756,952 - Sama_2292 0.29 -1.5 2,756,975 + Sama_2292 0.31 -2.1 2,756,975 + Sama_2292 0.31 -0.7 2,756,975 + Sama_2292 0.31 -1.2 2,756,975 + Sama_2292 0.31 -1.2 2,756,975 + Sama_2292 0.31 -2.1 2,756,976 - Sama_2292 0.31 +0.1 2,756,976 - Sama_2292 0.31 -0.7 2,756,976 - Sama_2292 0.31 -1.5 2,756,976 - Sama_2292 0.31 -2.5 2,756,995 + Sama_2292 0.32 -0.9 2,757,114 - Sama_2292 0.38 -2.6 2,757,160 + Sama_2292 0.41 +0.6 2,757,160 + Sama_2292 0.41 -1.2 2,757,215 - Sama_2292 0.44 -2.2 2,757,317 - Sama_2292 0.50 -2.6 2,757,329 + Sama_2292 0.51 +0.1 2,757,330 - Sama_2292 0.51 -1.2 2,757,330 - Sama_2292 0.51 -3.5
Or see this region's nucleotide sequence