Experiment: Motility assay, center cut sample 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0596 and Sama_0597 are separated by 453 nucleotides Sama_0597 and Sama_0598 are separated by 217 nucleotides Sama_0598 and Sama_0599 are separated by 122 nucleotides
Sama_0596: Sama_0596 - IS1004 transposase (RefSeq), at 747,054 to 747,500
_0596
Sama_0597: Sama_0597 - hypothetical protein (RefSeq), at 747,954 to 748,385
_0597
Sama_0598: Sama_0598 - inorganic pyrophosphatase (RefSeq), at 748,603 to 749,136
_0598
Sama_0599: Sama_0599 - fructose-1,6-bisphosphatase (RefSeq), at 749,259 to 750,221
_0599
Position (kb) 747
748
749 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 746.958 kb on - strand at 747.543 kb on + strand at 747.543 kb on + strand at 747.543 kb on + strand at 747.573 kb on - strand at 747.573 kb on - strand at 747.594 kb on - strand at 747.606 kb on + strand at 747.652 kb on - strand at 747.654 kb on + strand at 747.672 kb on - strand at 747.672 kb on - strand at 747.688 kb on - strand at 747.706 kb on + strand at 747.732 kb on + strand at 747.736 kb on - strand at 747.738 kb on + strand at 747.776 kb on + strand at 747.797 kb on + strand at 747.797 kb on + strand at 747.835 kb on + strand at 747.849 kb on + strand at 747.849 kb on + strand at 747.849 kb on + strand at 747.849 kb on + strand at 747.850 kb on - strand at 747.862 kb on + strand at 747.863 kb on - strand at 747.891 kb on + strand at 747.921 kb on - strand at 747.963 kb on - strand at 748.023 kb on - strand, within Sama_0597 at 748.023 kb on - strand, within Sama_0597 at 748.086 kb on - strand, within Sama_0597 at 748.173 kb on - strand, within Sama_0597 at 748.184 kb on + strand, within Sama_0597 at 748.253 kb on + strand, within Sama_0597 at 748.254 kb on - strand, within Sama_0597 at 748.260 kb on + strand, within Sama_0597 at 748.261 kb on - strand, within Sama_0597 at 748.263 kb on - strand, within Sama_0597 at 748.263 kb on - strand, within Sama_0597 at 748.304 kb on + strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.307 kb on + strand, within Sama_0597 at 748.308 kb on - strand, within Sama_0597 at 748.344 kb on + strand at 748.344 kb on + strand at 748.345 kb on - strand at 748.381 kb on + strand at 748.382 kb on - strand at 748.382 kb on - strand at 748.389 kb on + strand at 748.502 kb on + strand at 748.502 kb on + strand at 748.543 kb on + strand at 748.604 kb on + strand at 748.605 kb on - strand at 748.605 kb on - strand at 749.191 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.196 kb on - strand at 749.248 kb on + strand at 749.248 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility assay, center cut sample 2 remove 746,958 - -1.3 747,543 + -3.0 747,543 + +0.2 747,543 + -0.9 747,573 - +0.2 747,573 - -1.8 747,594 - +0.6 747,606 + +0.0 747,652 - +0.5 747,654 + -1.6 747,672 - +1.2 747,672 - -1.2 747,688 - -1.0 747,706 + -1.7 747,732 + -1.6 747,736 - -0.8 747,738 + +0.2 747,776 + -1.2 747,797 + -1.4 747,797 + -3.6 747,835 + -0.4 747,849 + -0.3 747,849 + -0.8 747,849 + -2.3 747,849 + -0.1 747,850 - -0.8 747,862 + +0.2 747,863 - -0.4 747,891 + +1.5 747,921 - +0.3 747,963 - +1.9 748,023 - Sama_0597 0.16 +3.0 748,023 - Sama_0597 0.16 +0.1 748,086 - Sama_0597 0.31 +3.7 748,173 - Sama_0597 0.51 +3.6 748,184 + Sama_0597 0.53 -0.1 748,253 + Sama_0597 0.69 -0.0 748,254 - Sama_0597 0.69 +4.3 748,260 + Sama_0597 0.71 +2.0 748,261 - Sama_0597 0.71 +2.3 748,263 - Sama_0597 0.72 +1.3 748,263 - Sama_0597 0.72 +3.1 748,304 + Sama_0597 0.81 +3.0 748,305 - Sama_0597 0.81 +2.8 748,305 - Sama_0597 0.81 +3.3 748,305 - Sama_0597 0.81 -0.6 748,305 - Sama_0597 0.81 +1.9 748,305 - Sama_0597 0.81 +3.9 748,307 + Sama_0597 0.82 +0.7 748,308 - Sama_0597 0.82 +3.4 748,344 + +3.9 748,344 + +2.1 748,345 - +0.7 748,381 + +2.5 748,382 - +3.6 748,382 - -1.4 748,389 + +4.3 748,502 + -0.3 748,502 + +2.5 748,543 + +2.2 748,604 + -0.1 748,605 - +0.6 748,605 - -2.3 749,191 - +0.0 749,194 - -0.6 749,194 - -0.7 749,194 - -0.3 749,194 - -0.3 749,194 - -0.5 749,194 - -2.3 749,196 - +0.9 749,248 + -1.4 749,248 + -1.6
Or see this region's nucleotide sequence