Strain Fitness in Shewanella amazonensis SB2B around Sama_0263

Experiment: Motility assay, center cut sample 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSama_0262 and Sama_0263 are separated by 68 nucleotidesSama_0263 and Sama_0264 are separated by 25 nucleotides Sama_0262: Sama_0262 - tRNA guanosine-2'-O-methyltransferase (RefSeq), at 301,630 to 302,355 _0262 Sama_0263: Sama_0263 - YjgF-like protein (RefSeq), at 302,424 to 302,807 _0263 Sama_0264: Sama_0264 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 302,833 to 304,932 _0264 Position (kb) 302 303Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 301.454 kb on + strandat 301.454 kb on + strandat 301.454 kb on + strandat 301.455 kb on - strandat 301.455 kb on - strandat 301.455 kb on - strandat 301.475 kb on + strandat 301.485 kb on + strandat 301.486 kb on - strandat 301.486 kb on - strandat 301.486 kb on - strandat 301.486 kb on - strandat 301.486 kb on - strandat 301.599 kb on + strandat 301.599 kb on + strandat 301.599 kb on + strandat 301.600 kb on - strandat 301.602 kb on - strandat 301.631 kb on + strandat 301.631 kb on + strandat 301.631 kb on + strandat 301.631 kb on + strandat 301.631 kb on + strandat 301.632 kb on - strandat 301.632 kb on - strandat 301.737 kb on + strand, within Sama_0262at 301.738 kb on - strand, within Sama_0262at 301.745 kb on + strand, within Sama_0262at 301.778 kb on + strand, within Sama_0262at 301.778 kb on + strand, within Sama_0262at 301.778 kb on + strand, within Sama_0262at 301.778 kb on + strand, within Sama_0262at 301.778 kb on + strand, within Sama_0262at 301.778 kb on + strand, within Sama_0262at 301.779 kb on - strand, within Sama_0262at 301.779 kb on - strand, within Sama_0262at 301.779 kb on - strand, within Sama_0262at 301.779 kb on - strand, within Sama_0262at 301.779 kb on - strand, within Sama_0262at 301.779 kb on - strand, within Sama_0262at 301.779 kb on - strand, within Sama_0262at 301.818 kb on - strand, within Sama_0262at 301.818 kb on - strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.844 kb on + strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.845 kb on - strand, within Sama_0262at 301.855 kb on - strand, within Sama_0262at 301.885 kb on + strand, within Sama_0262at 301.886 kb on - strand, within Sama_0262at 301.942 kb on - strand, within Sama_0262at 302.006 kb on - strand, within Sama_0262at 302.022 kb on + strand, within Sama_0262at 302.023 kb on - strand, within Sama_0262at 302.023 kb on - strand, within Sama_0262at 302.027 kb on + strand, within Sama_0262at 302.027 kb on + strand, within Sama_0262at 302.027 kb on + strand, within Sama_0262at 302.085 kb on + strand, within Sama_0262at 302.085 kb on + strand, within Sama_0262at 302.085 kb on + strand, within Sama_0262at 302.085 kb on + strand, within Sama_0262at 302.085 kb on + strand, within Sama_0262at 302.086 kb on - strand, within Sama_0262at 302.086 kb on - strand, within Sama_0262at 302.086 kb on - strand, within Sama_0262at 302.086 kb on - strand, within Sama_0262at 302.086 kb on - strand, within Sama_0262at 302.086 kb on - strand, within Sama_0262at 302.086 kb on - strand, within Sama_0262at 302.086 kb on - strand, within Sama_0262at 302.143 kb on - strand, within Sama_0262at 302.287 kb on + strandat 302.288 kb on - strandat 302.288 kb on - strandat 302.288 kb on - strandat 302.371 kb on + strandat 302.371 kb on + strandat 302.371 kb on + strandat 302.371 kb on + strandat 302.372 kb on - strandat 302.425 kb on + strandat 302.425 kb on + strandat 302.425 kb on + strandat 302.425 kb on + strandat 302.425 kb on + strandat 302.425 kb on + strandat 302.426 kb on - strandat 302.426 kb on - strandat 302.426 kb on - strandat 302.504 kb on - strand, within Sama_0263at 302.518 kb on + strand, within Sama_0263at 302.573 kb on + strand, within Sama_0263at 302.573 kb on + strand, within Sama_0263at 302.573 kb on + strand, within Sama_0263at 302.573 kb on + strand, within Sama_0263at 302.574 kb on - strand, within Sama_0263at 302.574 kb on - strand, within Sama_0263at 302.619 kb on + strand, within Sama_0263at 302.619 kb on + strand, within Sama_0263at 302.620 kb on - strand, within Sama_0263at 302.620 kb on - strand, within Sama_0263at 302.620 kb on - strand, within Sama_0263at 302.620 kb on - strand, within Sama_0263at 302.640 kb on + strand, within Sama_0263at 302.640 kb on + strand, within Sama_0263at 302.640 kb on + strand, within Sama_0263at 302.719 kb on + strand, within Sama_0263at 302.719 kb on + strand, within Sama_0263at 302.719 kb on + strand, within Sama_0263at 302.720 kb on - strand, within Sama_0263at 302.720 kb on - strand, within Sama_0263at 302.720 kb on - strand, within Sama_0263at 302.726 kb on + strand, within Sama_0263at 302.727 kb on - strand, within Sama_0263at 302.739 kb on + strand, within Sama_0263at 302.740 kb on - strand, within Sama_0263at 302.740 kb on - strand, within Sama_0263at 302.740 kb on - strand, within Sama_0263at 302.752 kb on + strand, within Sama_0263at 302.752 kb on + strand, within Sama_0263at 302.752 kb on + strand, within Sama_0263at 302.753 kb on - strand, within Sama_0263at 302.756 kb on + strand, within Sama_0263at 302.807 kb on + strandat 302.807 kb on + strandat 302.807 kb on + strandat 302.807 kb on + strandat 302.808 kb on - strandat 302.808 kb on - strandat 302.820 kb on + strandat 302.820 kb on + strandat 302.821 kb on - strandat 302.904 kb on + strandat 302.904 kb on + strandat 302.904 kb on + strandat 302.939 kb on + strandat 302.939 kb on + strandat 302.939 kb on + strandat 302.939 kb on + strandat 302.940 kb on - strandat 302.940 kb on - strandat 302.940 kb on - strandat 302.946 kb on + strandat 302.946 kb on + strandat 302.946 kb on + strandat 302.946 kb on + strandat 302.947 kb on - strandat 302.947 kb on - strandat 302.947 kb on - strandat 302.974 kb on - strandat 303.065 kb on + strand, within Sama_0264at 303.066 kb on - strand, within Sama_0264at 303.085 kb on - strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.104 kb on + strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.105 kb on - strand, within Sama_0264at 303.158 kb on - strand, within Sama_0264at 303.329 kb on - strand, within Sama_0264at 303.329 kb on - strand, within Sama_0264at 303.329 kb on - strand, within Sama_0264at 303.329 kb on - strand, within Sama_0264at 303.329 kb on - strand, within Sama_0264at 303.329 kb on - strand, within Sama_0264at 303.353 kb on - strand, within Sama_0264at 303.353 kb on - strand, within Sama_0264at 303.353 kb on - strand, within Sama_0264at 303.353 kb on - strand, within Sama_0264at 303.399 kb on + strand, within Sama_0264at 303.399 kb on + strand, within Sama_0264at 303.459 kb on + strand, within Sama_0264at 303.459 kb on + strand, within Sama_0264at 303.460 kb on - strand, within Sama_0264at 303.511 kb on + strand, within Sama_0264at 303.765 kb on - strand, within Sama_0264

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Per-strain Table

Position Strand Gene LocusTag Fraction Motility assay, center cut sample 2
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301,454 + +0.6
301,454 + +0.4
301,454 + -1.6
301,455 - +0.3
301,455 - +0.9
301,455 - +0.4
301,475 + -0.2
301,485 + +0.6
301,486 - -1.9
301,486 - -0.6
301,486 - +0.5
301,486 - +2.7
301,486 - -1.2
301,599 + -0.5
301,599 + +0.5
301,599 + -2.5
301,600 - +1.0
301,602 - +0.3
301,631 + -1.6
301,631 + +2.4
301,631 + -0.7
301,631 + +3.0
301,631 + -0.7
301,632 - +0.1
301,632 - +3.6
301,737 + Sama_0262 0.15 -1.1
301,738 - Sama_0262 0.15 +0.8
301,745 + Sama_0262 0.16 -0.1
301,778 + Sama_0262 0.20 -1.9
301,778 + Sama_0262 0.20 -0.7
301,778 + Sama_0262 0.20 -0.1
301,778 + Sama_0262 0.20 +0.5
301,778 + Sama_0262 0.20 -0.7
301,778 + Sama_0262 0.20 +0.2
301,779 - Sama_0262 0.21 +1.3
301,779 - Sama_0262 0.21 +0.3
301,779 - Sama_0262 0.21 -1.0
301,779 - Sama_0262 0.21 +1.1
301,779 - Sama_0262 0.21 -2.8
301,779 - Sama_0262 0.21 -1.9
301,779 - Sama_0262 0.21 +2.7
301,818 - Sama_0262 0.26 +2.1
301,818 - Sama_0262 0.26 +0.8
301,844 + Sama_0262 0.29 -1.2
301,844 + Sama_0262 0.29 +0.8
301,844 + Sama_0262 0.29 -1.2
301,844 + Sama_0262 0.29 -0.5
301,844 + Sama_0262 0.29 +0.8
301,844 + Sama_0262 0.29 +1.6
301,844 + Sama_0262 0.29 +1.4
301,844 + Sama_0262 0.29 -1.2
301,844 + Sama_0262 0.29 -1.5
301,844 + Sama_0262 0.29 -0.2
301,844 + Sama_0262 0.29 -1.1
301,844 + Sama_0262 0.29 -0.0
301,844 + Sama_0262 0.29 -0.9
301,844 + Sama_0262 0.29 -2.4
301,845 - Sama_0262 0.30 -0.5
301,845 - Sama_0262 0.30 -0.9
301,845 - Sama_0262 0.30 -1.6
301,845 - Sama_0262 0.30 -0.7
301,845 - Sama_0262 0.30 -1.3
301,845 - Sama_0262 0.30 -0.3
301,845 - Sama_0262 0.30 +1.4
301,845 - Sama_0262 0.30 -0.0
301,845 - Sama_0262 0.30 -1.9
301,845 - Sama_0262 0.30 +2.2
301,855 - Sama_0262 0.31 -1.9
301,885 + Sama_0262 0.35 +0.8
301,886 - Sama_0262 0.35 +0.6
301,942 - Sama_0262 0.43 +0.3
302,006 - Sama_0262 0.52 -0.3
302,022 + Sama_0262 0.54 -0.2
302,023 - Sama_0262 0.54 -1.5
302,023 - Sama_0262 0.54 -0.5
302,027 + Sama_0262 0.55 +0.2
302,027 + Sama_0262 0.55 -1.1
302,027 + Sama_0262 0.55 +0.8
302,085 + Sama_0262 0.63 -0.6
302,085 + Sama_0262 0.63 -0.0
302,085 + Sama_0262 0.63 +0.6
302,085 + Sama_0262 0.63 +1.3
302,085 + Sama_0262 0.63 -1.0
302,086 - Sama_0262 0.63 -1.5
302,086 - Sama_0262 0.63 -1.9
302,086 - Sama_0262 0.63 -0.5
302,086 - Sama_0262 0.63 -1.8
302,086 - Sama_0262 0.63 -1.6
302,086 - Sama_0262 0.63 +0.0
302,086 - Sama_0262 0.63 +1.3
302,086 - Sama_0262 0.63 +1.8
302,143 - Sama_0262 0.71 -1.2
302,287 + +3.5
302,288 - +0.3
302,288 - +0.6
302,288 - -0.5
302,371 + +1.8
302,371 + +0.1
302,371 + -0.9
302,371 + +1.1
302,372 - -0.3
302,425 + -0.3
302,425 + -0.3
302,425 + +0.5
302,425 + +1.0
302,425 + -1.9
302,425 + +0.0
302,426 - +1.7
302,426 - +0.6
302,426 - -0.0
302,504 - Sama_0263 0.21 -1.5
302,518 + Sama_0263 0.24 -1.5
302,573 + Sama_0263 0.39 -1.5
302,573 + Sama_0263 0.39 -2.5
302,573 + Sama_0263 0.39 +0.5
302,573 + Sama_0263 0.39 -1.1
302,574 - Sama_0263 0.39 -2.0
302,574 - Sama_0263 0.39 -0.9
302,619 + Sama_0263 0.51 -0.7
302,619 + Sama_0263 0.51 +0.0
302,620 - Sama_0263 0.51 -2.8
302,620 - Sama_0263 0.51 -1.3
302,620 - Sama_0263 0.51 -1.5
302,620 - Sama_0263 0.51 -0.7
302,640 + Sama_0263 0.56 -2.0
302,640 + Sama_0263 0.56 -1.5
302,640 + Sama_0263 0.56 -1.5
302,719 + Sama_0263 0.77 -1.5
302,719 + Sama_0263 0.77 +1.2
302,719 + Sama_0263 0.77 -1.7
302,720 - Sama_0263 0.77 -0.4
302,720 - Sama_0263 0.77 -2.4
302,720 - Sama_0263 0.77 -0.2
302,726 + Sama_0263 0.79 -2.5
302,727 - Sama_0263 0.79 +0.3
302,739 + Sama_0263 0.82 -2.9
302,740 - Sama_0263 0.82 -0.0
302,740 - Sama_0263 0.82 +0.5
302,740 - Sama_0263 0.82 -1.6
302,752 + Sama_0263 0.85 -1.5
302,752 + Sama_0263 0.85 +0.8
302,752 + Sama_0263 0.85 -0.7
302,753 - Sama_0263 0.86 -0.2
302,756 + Sama_0263 0.86 -0.5
302,807 + -1.2
302,807 + -1.0
302,807 + -0.5
302,807 + -1.0
302,808 - -0.2
302,808 - -3.0
302,820 + -1.3
302,820 + -2.1
302,821 - -0.9
302,904 + +0.3
302,904 + -0.4
302,904 + +0.8
302,939 + -2.5
302,939 + +1.0
302,939 + -0.2
302,939 + -1.2
302,940 - +2.6
302,940 - +1.1
302,940 - -0.2
302,946 + +0.8
302,946 + -1.5
302,946 + +0.6
302,946 + -1.5
302,947 - +1.8
302,947 - +2.1
302,947 - -0.4
302,974 - -1.5
303,065 + Sama_0264 0.11 +1.4
303,066 - Sama_0264 0.11 -0.0
303,085 - Sama_0264 0.12 +1.4
303,104 + Sama_0264 0.13 -0.5
303,104 + Sama_0264 0.13 -0.9
303,104 + Sama_0264 0.13 -0.8
303,104 + Sama_0264 0.13 -0.7
303,104 + Sama_0264 0.13 -2.1
303,104 + Sama_0264 0.13 -0.4
303,104 + Sama_0264 0.13 +0.2
303,104 + Sama_0264 0.13 +0.3
303,104 + Sama_0264 0.13 +1.3
303,104 + Sama_0264 0.13 -1.9
303,105 - Sama_0264 0.13 +1.3
303,105 - Sama_0264 0.13 -0.5
303,105 - Sama_0264 0.13 -0.7
303,105 - Sama_0264 0.13 +1.3
303,105 - Sama_0264 0.13 -1.3
303,105 - Sama_0264 0.13 -0.2
303,105 - Sama_0264 0.13 +1.5
303,105 - Sama_0264 0.13 +3.2
303,105 - Sama_0264 0.13 +0.8
303,105 - Sama_0264 0.13 +2.1
303,105 - Sama_0264 0.13 +2.4
303,158 - Sama_0264 0.15 -1.3
303,329 - Sama_0264 0.24 +1.0
303,329 - Sama_0264 0.24 +1.3
303,329 - Sama_0264 0.24 -0.7
303,329 - Sama_0264 0.24 -0.1
303,329 - Sama_0264 0.24 +1.1
303,329 - Sama_0264 0.24 -0.6
303,353 - Sama_0264 0.25 +0.3
303,353 - Sama_0264 0.25 +0.8
303,353 - Sama_0264 0.25 +0.9
303,353 - Sama_0264 0.25 -1.7
303,399 + Sama_0264 0.27 +0.7
303,399 + Sama_0264 0.27 +1.6
303,459 + Sama_0264 0.30 -2.2
303,459 + Sama_0264 0.30 +0.0
303,460 - Sama_0264 0.30 +0.1
303,511 + Sama_0264 0.32 +1.0
303,765 - Sama_0264 0.44 +1.5

Or see this region's nucleotide sequence