Strain Fitness in Shewanella oneidensis MR-1 around SO2347

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2346 and gapA-3 are separated by 205 nucleotidesgapA-3 and tRNA-Gly-7 are separated by 296 nucleotidestRNA-Gly-7 and tRNA-Cys-2 are separated by 20 nucleotidestRNA-Cys-2 and tRNA-Leu-6 are separated by 49 nucleotidestRNA-Leu-6 and SO2349 are separated by 23 nucleotidesSO2349 and aspC-1 are separated by 158 nucleotides SO2346: SO2346 - conserved hypothetical protein (NCBI ptt file), at 2,454,029 to 2,454,907 SO2346 SO2347: gapA-3 - glyceraldehyde 3-phosphate dehydrogenase (NCBI ptt file), at 2,455,113 to 2,456,546 gapA-3 SO_t041: tRNA-Gly-7 - tRNA-Gly (RefSeq), at 2,456,843 to 2,456,918 tRNA-Gly-7 SO_t042: tRNA-Cys-2 - tRNA-Cys (RefSeq), at 2,456,939 to 2,457,012 tRNA-Cys-2 SO_t043: tRNA-Leu-6 - tRNA-Leu (RefSeq), at 2,457,062 to 2,457,148 tRNA-Leu-6 SO2349: SO2349 - hypothetical protein (NCBI ptt file), at 2,457,172 to 2,457,270 SO2349 SO2350: aspC-1 - aspartate aminotransferase (NCBI ptt file), at 2,457,429 to 2,458,622 aspC-1 Position (kb) 2455 2456 2457Strain fitness (log2 ratio) -3 -2 -1 0 1at 2454.289 kb on - strand, within SO2346at 2454.300 kb on + strand, within SO2346at 2454.300 kb on + strand, within SO2346at 2454.308 kb on - strand, within SO2346at 2454.309 kb on + strand, within SO2346at 2454.361 kb on + strand, within SO2346at 2454.369 kb on - strand, within SO2346at 2454.369 kb on - strand, within SO2346at 2454.411 kb on - strand, within SO2346at 2454.591 kb on + strand, within SO2346at 2454.606 kb on + strand, within SO2346at 2454.639 kb on + strand, within SO2346at 2454.639 kb on + strand, within SO2346at 2454.647 kb on - strand, within SO2346at 2454.652 kb on - strand, within SO2346at 2454.657 kb on - strand, within SO2346at 2454.680 kb on + strand, within SO2346at 2454.690 kb on + strand, within SO2346at 2454.719 kb on - strand, within SO2346at 2454.765 kb on + strand, within SO2346at 2454.765 kb on + strand, within SO2346at 2455.062 kb on + strandat 2455.107 kb on + strandat 2455.190 kb on + strandat 2455.340 kb on - strand, within gapA-3at 2455.354 kb on + strand, within gapA-3at 2455.362 kb on - strand, within gapA-3at 2455.400 kb on - strand, within gapA-3at 2455.833 kb on + strand, within gapA-3at 2455.839 kb on + strand, within gapA-3at 2456.000 kb on + strand, within gapA-3at 2456.065 kb on - strand, within gapA-3at 2456.106 kb on - strand, within gapA-3at 2456.106 kb on - strand, within gapA-3at 2456.124 kb on + strand, within gapA-3at 2456.703 kb on + strandat 2456.781 kb on + strandat 2457.364 kb on + strandat 2457.364 kb on + strandat 2457.443 kb on + strandat 2457.451 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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2,454,289 - SO2346 0.30 -0.3
2,454,300 + SO2346 0.31 -0.3
2,454,300 + SO2346 0.31 -0.2
2,454,308 - SO2346 0.32 -0.6
2,454,309 + SO2346 0.32 +0.2
2,454,361 + SO2346 0.38 -0.6
2,454,369 - SO2346 0.39 -0.3
2,454,369 - SO2346 0.39 +0.5
2,454,411 - SO2346 0.43 -0.1
2,454,591 + SO2346 0.64 -1.1
2,454,606 + SO2346 0.66 +0.6
2,454,639 + SO2346 0.69 -0.3
2,454,639 + SO2346 0.69 +0.5
2,454,647 - SO2346 0.70 +1.2
2,454,652 - SO2346 0.71 -2.4
2,454,657 - SO2346 0.71 +0.2
2,454,680 + SO2346 0.74 -0.6
2,454,690 + SO2346 0.75 +0.1
2,454,719 - SO2346 0.78 +1.0
2,454,765 + SO2346 0.84 -0.2
2,454,765 + SO2346 0.84 -1.1
2,455,062 + -2.1
2,455,107 + -0.3
2,455,190 + -2.2
2,455,340 - gapA-3 SO2347 0.16 -2.6
2,455,354 + gapA-3 SO2347 0.17 -0.7
2,455,362 - gapA-3 SO2347 0.17 -1.7
2,455,400 - gapA-3 SO2347 0.20 -2.8
2,455,833 + gapA-3 SO2347 0.50 -1.7
2,455,839 + gapA-3 SO2347 0.51 -2.3
2,456,000 + gapA-3 SO2347 0.62 +0.3
2,456,065 - gapA-3 SO2347 0.66 -2.5
2,456,106 - gapA-3 SO2347 0.69 -1.3
2,456,106 - gapA-3 SO2347 0.69 -1.1
2,456,124 + gapA-3 SO2347 0.71 -1.7
2,456,703 + +0.3
2,456,781 + +0.0
2,457,364 + -0.1
2,457,364 + +0.1
2,457,443 + -0.1
2,457,451 - +1.3

Or see this region's nucleotide sequence