Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt astE and SO2339 are separated by 162 nucleotides SO2339 and SO2340 are separated by 3 nucleotides SO2340 and SO2341 are separated by 9 nucleotides
SO2338: astE - succinylglutamate desuccinylase (NCBI ptt file), at 2,445,175 to 2,446,209
astE
SO2339: SO2339 - alpha keto acid dehydrogenase complex, E1 component, alpha subunit (NCBI ptt file), at 2,446,372 to 2,447,550
SO2339
SO2340: SO2340 - alpha keto acid dehydrogenase complex, E1 component, beta subunit (NCBI ptt file), at 2,447,554 to 2,448,531
SO2340
SO2341: SO2341 - alpha keto acid dehydrogenase complex, E2 component (NCBI ptt file), at 2,448,541 to 2,450,148
SO2341
Position (kb)
2446
2447
2448 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2445.527 kb on - strand, within astE at 2445.534 kb on + strand, within astE at 2445.560 kb on - strand, within astE at 2445.730 kb on - strand, within astE at 2445.732 kb on + strand, within astE at 2445.839 kb on + strand, within astE at 2445.863 kb on - strand, within astE at 2445.934 kb on + strand, within astE at 2446.184 kb on + strand at 2446.818 kb on - strand, within SO2339 at 2446.862 kb on + strand, within SO2339 at 2446.868 kb on - strand, within SO2339 at 2446.968 kb on - strand, within SO2339 at 2447.090 kb on - strand, within SO2339 at 2447.151 kb on + strand, within SO2339 at 2447.160 kb on - strand, within SO2339 at 2447.196 kb on + strand, within SO2339 at 2447.420 kb on - strand, within SO2339 at 2447.675 kb on + strand, within SO2340 at 2447.683 kb on - strand, within SO2340 at 2447.731 kb on + strand, within SO2340 at 2447.739 kb on - strand, within SO2340 at 2447.773 kb on + strand, within SO2340 at 2447.870 kb on - strand, within SO2340 at 2447.876 kb on + strand, within SO2340 at 2447.876 kb on + strand, within SO2340 at 2447.884 kb on - strand, within SO2340 at 2447.891 kb on + strand, within SO2340 at 2447.899 kb on - strand, within SO2340 at 2447.937 kb on + strand, within SO2340 at 2448.005 kb on + strand, within SO2340 at 2448.008 kb on - strand, within SO2340 at 2448.135 kb on + strand, within SO2340 at 2448.158 kb on - strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.160 kb on + strand, within SO2340 at 2448.168 kb on - strand, within SO2340 at 2448.168 kb on - strand, within SO2340 at 2448.296 kb on - strand, within SO2340 at 2448.418 kb on + strand, within SO2340
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 2,445,527 - astE SO2338 0.34 +0.7 2,445,534 + astE SO2338 0.35 -2.1 2,445,560 - astE SO2338 0.37 -1.1 2,445,730 - astE SO2338 0.54 -1.6 2,445,732 + astE SO2338 0.54 -2.1 2,445,839 + astE SO2338 0.64 -1.2 2,445,863 - astE SO2338 0.66 -3.5 2,445,934 + astE SO2338 0.73 -1.0 2,446,184 + -0.9 2,446,818 - SO2339 0.38 -2.6 2,446,862 + SO2339 0.42 -3.7 2,446,868 - SO2339 0.42 -2.7 2,446,968 - SO2339 0.51 -1.5 2,447,090 - SO2339 0.61 -3.2 2,447,151 + SO2339 0.66 -0.8 2,447,160 - SO2339 0.67 -2.9 2,447,196 + SO2339 0.70 -3.5 2,447,420 - SO2339 0.89 -4.9 2,447,675 + SO2340 0.12 -4.3 2,447,683 - SO2340 0.13 -4.5 2,447,731 + SO2340 0.18 -2.3 2,447,739 - SO2340 0.19 -3.8 2,447,773 + SO2340 0.22 -5.5 2,447,870 - SO2340 0.32 -3.9 2,447,876 + SO2340 0.33 -2.4 2,447,876 + SO2340 0.33 -3.1 2,447,884 - SO2340 0.34 -2.6 2,447,891 + SO2340 0.34 -4.1 2,447,899 - SO2340 0.35 -2.3 2,447,937 + SO2340 0.39 -4.2 2,448,005 + SO2340 0.46 -2.6 2,448,008 - SO2340 0.46 -4.6 2,448,135 + SO2340 0.59 -1.9 2,448,158 - SO2340 0.62 -5.1 2,448,160 + SO2340 0.62 -4.8 2,448,160 + SO2340 0.62 -4.4 2,448,160 + SO2340 0.62 -3.8 2,448,160 + SO2340 0.62 -3.0 2,448,168 - SO2340 0.63 -5.5 2,448,168 - SO2340 0.63 -2.7 2,448,296 - SO2340 0.76 -2.1 2,448,418 + SO2340 0.88 -1.9
Or see this region's nucleotide sequence