Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pccB-1 and ivd are separated by 116 nucleotides ivd and SO1898 are separated by 144 nucleotides SO1898 and SO1899 are separated by 260 nucleotides
SO1896: pccB-1 - 3-methylcrotonyl CoA carboxylase, beta subunit (NCBI ptt file), at 1,993,425 to 1,995,032
pccB-1
SO1897: ivd - isovaleryl-CoA dehydrogenase (NCBI ptt file), at 1,995,149 to 1,996,318
ivd
SO1898: SO1898 - transcriptional regulator, putative (NCBI ptt file), at 1,996,463 to 1,996,867
SO1898
SO1899: SO1899 - conserved hypothetical protein (NCBI ptt file), at 1,997,128 to 1,998,843
SO1899
Position (kb)
1995
1996
1997 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1994.261 kb on + strand, within pccB-1 at 1994.269 kb on - strand, within pccB-1 at 1994.269 kb on - strand, within pccB-1 at 1994.469 kb on + strand, within pccB-1 at 1994.472 kb on - strand, within pccB-1 at 1994.477 kb on - strand, within pccB-1 at 1994.503 kb on + strand, within pccB-1 at 1994.927 kb on - strand at 1995.137 kb on + strand at 1995.142 kb on + strand at 1995.169 kb on - strand at 1995.204 kb on - strand at 1995.218 kb on + strand at 1995.218 kb on + strand at 1995.419 kb on + strand, within ivd at 1995.427 kb on - strand, within ivd at 1995.427 kb on - strand, within ivd at 1995.427 kb on - strand, within ivd at 1995.563 kb on - strand, within ivd at 1995.651 kb on + strand, within ivd at 1995.681 kb on - strand, within ivd at 1995.681 kb on - strand, within ivd at 1995.776 kb on - strand, within ivd at 1995.987 kb on + strand, within ivd at 1996.063 kb on - strand, within ivd at 1996.264 kb on + strand at 1996.299 kb on + strand at 1996.309 kb on - strand at 1996.534 kb on - strand, within SO1898 at 1996.599 kb on + strand, within SO1898 at 1996.599 kb on + strand, within SO1898 at 1996.599 kb on + strand, within SO1898 at 1996.599 kb on + strand, within SO1898 at 1996.607 kb on - strand, within SO1898 at 1996.622 kb on - strand, within SO1898 at 1996.622 kb on - strand, within SO1898 at 1996.668 kb on - strand, within SO1898 at 1996.693 kb on + strand, within SO1898 at 1996.693 kb on + strand, within SO1898 at 1996.717 kb on + strand, within SO1898 at 1996.723 kb on - strand, within SO1898 at 1996.774 kb on - strand, within SO1898 at 1996.873 kb on - strand at 1996.873 kb on - strand at 1997.057 kb on + strand at 1997.087 kb on - strand at 1997.087 kb on - strand at 1997.134 kb on - strand at 1997.134 kb on - strand at 1997.134 kb on - strand at 1997.138 kb on + strand at 1997.146 kb on - strand at 1997.146 kb on - strand at 1997.185 kb on + strand at 1997.193 kb on - strand at 1997.193 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 1,994,261 + pccB-1 SO1896 0.52 +2.4 1,994,269 - pccB-1 SO1896 0.52 -2.6 1,994,269 - pccB-1 SO1896 0.52 -1.3 1,994,469 + pccB-1 SO1896 0.65 +1.0 1,994,472 - pccB-1 SO1896 0.65 -0.9 1,994,477 - pccB-1 SO1896 0.65 -0.6 1,994,503 + pccB-1 SO1896 0.67 -0.0 1,994,927 - -1.1 1,995,137 + -2.4 1,995,142 + -1.3 1,995,169 - +0.2 1,995,204 - -0.3 1,995,218 + -2.3 1,995,218 + -2.6 1,995,419 + ivd SO1897 0.23 -0.0 1,995,427 - ivd SO1897 0.24 -0.4 1,995,427 - ivd SO1897 0.24 +0.4 1,995,427 - ivd SO1897 0.24 +0.2 1,995,563 - ivd SO1897 0.35 +0.0 1,995,651 + ivd SO1897 0.43 +0.3 1,995,681 - ivd SO1897 0.45 -0.2 1,995,681 - ivd SO1897 0.45 +0.9 1,995,776 - ivd SO1897 0.54 +1.3 1,995,987 + ivd SO1897 0.72 -0.3 1,996,063 - ivd SO1897 0.78 +0.5 1,996,264 + -0.6 1,996,299 + -0.4 1,996,309 - +0.1 1,996,534 - SO1898 0.18 -0.7 1,996,599 + SO1898 0.34 -1.2 1,996,599 + SO1898 0.34 -0.1 1,996,599 + SO1898 0.34 -0.7 1,996,599 + SO1898 0.34 -0.7 1,996,607 - SO1898 0.36 -0.6 1,996,622 - SO1898 0.39 +0.1 1,996,622 - SO1898 0.39 -0.3 1,996,668 - SO1898 0.51 -1.6 1,996,693 + SO1898 0.57 +0.1 1,996,693 + SO1898 0.57 -1.4 1,996,717 + SO1898 0.63 -1.8 1,996,723 - SO1898 0.64 +0.3 1,996,774 - SO1898 0.77 -1.5 1,996,873 - -3.4 1,996,873 - -1.0 1,997,057 + -0.9 1,997,087 - -0.2 1,997,087 - +0.6 1,997,134 - +0.7 1,997,134 - -0.8 1,997,134 - +2.2 1,997,138 + +0.1 1,997,146 - -0.3 1,997,146 - +0.2 1,997,185 + -0.3 1,997,193 - -0.1 1,997,193 - +0.9
Or see this region's nucleotide sequence