Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrD-2 and nqrE-2 are separated by 5 nucleotides nqrE-2 and nqrF-2 are separated by 46 nucleotides nqrF-2 and apbE are separated by 99 nucleotides
SO1106: nqrD-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 1,149,550 to 1,150,182
nqrD-2
SO1107: nqrE-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 1,150,188 to 1,150,796
nqrE-2
SO1108: nqrF-2 - NADH:ubiquinone oxidoreductase, Na translocating, beta subunit (NCBI ptt file), at 1,150,843 to 1,152,099
nqrF-2
SO1109: apbE - thiamin biosynthesis lipoprotein ApbE (NCBI ptt file), at 1,152,199 to 1,153,242
apbE
Position (kb) 1150
1151
1152
1153 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1150.157 kb on - strand at 1150.323 kb on + strand, within nqrE-2 at 1150.427 kb on - strand, within nqrE-2 at 1150.539 kb on + strand, within nqrE-2 at 1150.556 kb on - strand, within nqrE-2 at 1150.638 kb on + strand, within nqrE-2 at 1152.137 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 1,150,157 - -2.6 1,150,323 + nqrE-2 SO1107 0.22 -4.0 1,150,427 - nqrE-2 SO1107 0.39 -2.7 1,150,539 + nqrE-2 SO1107 0.58 -4.0 1,150,556 - nqrE-2 SO1107 0.60 -1.1 1,150,638 + nqrE-2 SO1107 0.74 -0.9 1,152,137 + +0.7
Or see this region's nucleotide sequence