Experiment: Motility assay, center cut sample 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhA and flhB are separated by 99 nucleotides flhB and fliR are separated by 1 nucleotides fliR and Sama_2286 overlap by 1 nucleotides
Sama_2283: flhA - flagellar biosynthesis protein FlhA (RefSeq), at 2,748,828 to 2,750,927
flhA
Sama_2284: flhB - flagellar biosynthesis protein FlhB (RefSeq), at 2,751,027 to 2,752,160
flhB
Sama_2285: fliR - flagellar biosynthesis protein FliR (RefSeq), at 2,752,162 to 2,752,950
fliR
Sama_2286: Sama_2286 - flagellar biosynthetic protein FliQ (RefSeq), at 2,752,950 to 2,753,219
_2286
Position (kb)
2751
2752
2753 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2750.027 kb on + strand, within flhA at 2750.028 kb on - strand, within flhA at 2750.030 kb on + strand, within flhA at 2750.030 kb on + strand, within flhA at 2750.031 kb on - strand, within flhA at 2750.031 kb on - strand, within flhA at 2750.031 kb on - strand, within flhA at 2750.031 kb on - strand, within flhA at 2750.204 kb on + strand, within flhA at 2750.205 kb on - strand, within flhA at 2750.206 kb on + strand, within flhA at 2750.207 kb on - strand, within flhA at 2750.207 kb on - strand, within flhA at 2750.207 kb on - strand, within flhA at 2750.207 kb on - strand, within flhA at 2750.207 kb on - strand, within flhA at 2750.225 kb on + strand, within flhA at 2750.226 kb on - strand, within flhA at 2750.226 kb on - strand, within flhA at 2750.226 kb on - strand, within flhA at 2750.226 kb on - strand, within flhA at 2750.249 kb on + strand, within flhA at 2750.250 kb on - strand, within flhA at 2750.276 kb on + strand, within flhA at 2750.277 kb on - strand, within flhA at 2750.277 kb on - strand, within flhA at 2750.277 kb on - strand, within flhA at 2750.277 kb on - strand, within flhA at 2750.277 kb on - strand, within flhA at 2750.405 kb on + strand, within flhA at 2750.405 kb on + strand, within flhA at 2750.405 kb on + strand, within flhA at 2750.405 kb on + strand, within flhA at 2750.406 kb on - strand, within flhA at 2750.417 kb on + strand, within flhA at 2750.532 kb on - strand, within flhA at 2750.532 kb on - strand, within flhA at 2750.538 kb on + strand, within flhA at 2750.564 kb on - strand, within flhA at 2750.607 kb on - strand, within flhA at 2750.643 kb on + strand, within flhA at 2750.644 kb on - strand, within flhA at 2750.644 kb on - strand, within flhA at 2750.654 kb on + strand, within flhA at 2750.739 kb on - strand at 2750.739 kb on - strand at 2750.740 kb on + strand at 2750.741 kb on - strand at 2750.741 kb on - strand at 2750.758 kb on - strand at 2750.758 kb on - strand at 2750.758 kb on - strand at 2750.826 kb on + strand at 2750.827 kb on - strand at 2750.827 kb on - strand at 2750.827 kb on - strand at 2750.827 kb on - strand at 2750.827 kb on - strand at 2750.827 kb on - strand at 2750.960 kb on - strand at 2750.983 kb on + strand at 2750.984 kb on - strand at 2751.015 kb on + strand at 2751.015 kb on + strand at 2751.016 kb on - strand at 2751.017 kb on + strand at 2751.018 kb on - strand at 2751.018 kb on - strand at 2751.032 kb on - strand at 2751.032 kb on - strand at 2751.035 kb on + strand at 2751.036 kb on - strand at 2751.036 kb on - strand at 2751.121 kb on + strand at 2751.121 kb on + strand at 2751.122 kb on - strand at 2751.129 kb on + strand at 2751.130 kb on - strand at 2751.130 kb on - strand at 2751.146 kb on + strand, within flhB at 2751.182 kb on + strand, within flhB at 2751.182 kb on + strand, within flhB at 2751.182 kb on + strand, within flhB at 2751.260 kb on + strand, within flhB at 2751.323 kb on - strand, within flhB at 2751.323 kb on - strand, within flhB at 2751.361 kb on - strand, within flhB at 2751.361 kb on - strand, within flhB at 2751.592 kb on - strand, within flhB at 2751.636 kb on + strand, within flhB at 2751.756 kb on - strand, within flhB at 2751.831 kb on + strand, within flhB at 2751.831 kb on + strand, within flhB at 2751.831 kb on + strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.921 kb on - strand, within flhB at 2751.921 kb on - strand, within flhB at 2751.921 kb on - strand, within flhB at 2752.130 kb on + strand at 2752.146 kb on - strand at 2752.282 kb on - strand, within fliR at 2752.314 kb on + strand, within fliR at 2752.315 kb on - strand, within fliR at 2752.315 kb on - strand, within fliR at 2752.319 kb on - strand, within fliR at 2752.319 kb on - strand, within fliR at 2752.319 kb on - strand, within fliR at 2752.418 kb on + strand, within fliR at 2752.418 kb on + strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.446 kb on - strand, within fliR at 2752.446 kb on - strand, within fliR at 2752.446 kb on - strand, within fliR at 2752.446 kb on - strand, within fliR at 2752.450 kb on - strand, within fliR at 2752.471 kb on - strand, within fliR at 2752.471 kb on - strand, within fliR at 2752.485 kb on + strand, within fliR at 2752.780 kb on + strand, within fliR at 2752.781 kb on - strand, within fliR at 2752.845 kb on + strand, within fliR at 2752.845 kb on + strand, within fliR at 2752.846 kb on - strand, within fliR at 2752.846 kb on - strand, within fliR at 2752.872 kb on + strand at 2752.873 kb on - strand at 2752.873 kb on - strand at 2752.905 kb on + strand at 2752.905 kb on + strand at 2752.905 kb on + strand at 2752.905 kb on + strand at 2752.905 kb on + strand at 2752.906 kb on - strand at 2752.906 kb on - strand at 2752.908 kb on - strand at 2752.908 kb on - strand at 2752.918 kb on - strand at 2752.918 kb on - strand at 2752.918 kb on - strand at 2752.960 kb on - strand at 2753.147 kb on + strand, within Sama_2286 at 2753.148 kb on - strand, within Sama_2286 at 2753.148 kb on - strand, within Sama_2286 at 2753.148 kb on - strand, within Sama_2286
Per-strain Table
Position Strand Gene LocusTag Fraction Motility assay, center cut sample 1 remove 2,750,027 + flhA Sama_2283 0.57 -1.0 2,750,028 - flhA Sama_2283 0.57 -3.0 2,750,030 + flhA Sama_2283 0.57 -0.8 2,750,030 + flhA Sama_2283 0.57 -2.6 2,750,031 - flhA Sama_2283 0.57 -1.2 2,750,031 - flhA Sama_2283 0.57 -1.9 2,750,031 - flhA Sama_2283 0.57 -0.5 2,750,031 - flhA Sama_2283 0.57 -1.2 2,750,204 + flhA Sama_2283 0.66 -2.3 2,750,205 - flhA Sama_2283 0.66 +0.2 2,750,206 + flhA Sama_2283 0.66 -2.0 2,750,207 - flhA Sama_2283 0.66 -2.2 2,750,207 - flhA Sama_2283 0.66 -2.4 2,750,207 - flhA Sama_2283 0.66 -2.2 2,750,207 - flhA Sama_2283 0.66 -1.5 2,750,207 - flhA Sama_2283 0.66 -1.2 2,750,225 + flhA Sama_2283 0.67 -2.4 2,750,226 - flhA Sama_2283 0.67 -2.2 2,750,226 - flhA Sama_2283 0.67 -2.3 2,750,226 - flhA Sama_2283 0.67 -4.8 2,750,226 - flhA Sama_2283 0.67 -1.9 2,750,249 + flhA Sama_2283 0.68 -2.4 2,750,250 - flhA Sama_2283 0.68 -3.0 2,750,276 + flhA Sama_2283 0.69 -0.2 2,750,277 - flhA Sama_2283 0.69 -1.3 2,750,277 - flhA Sama_2283 0.69 -2.0 2,750,277 - flhA Sama_2283 0.69 -1.8 2,750,277 - flhA Sama_2283 0.69 -0.2 2,750,277 - flhA Sama_2283 0.69 -0.5 2,750,405 + flhA Sama_2283 0.75 -0.2 2,750,405 + flhA Sama_2283 0.75 +0.2 2,750,405 + flhA Sama_2283 0.75 +0.2 2,750,405 + flhA Sama_2283 0.75 +0.2 2,750,406 - flhA Sama_2283 0.75 -2.1 2,750,417 + flhA Sama_2283 0.76 -0.8 2,750,532 - flhA Sama_2283 0.81 -1.5 2,750,532 - flhA Sama_2283 0.81 -2.0 2,750,538 + flhA Sama_2283 0.81 -1.2 2,750,564 - flhA Sama_2283 0.83 -2.3 2,750,607 - flhA Sama_2283 0.85 -3.5 2,750,643 + flhA Sama_2283 0.86 -1.2 2,750,644 - flhA Sama_2283 0.86 -1.0 2,750,644 - flhA Sama_2283 0.86 -1.3 2,750,654 + flhA Sama_2283 0.87 -2.3 2,750,739 - -1.3 2,750,739 - -2.4 2,750,740 + -2.2 2,750,741 - -1.6 2,750,741 - -2.0 2,750,758 - -2.3 2,750,758 - -0.8 2,750,758 - -1.5 2,750,826 + -0.2 2,750,827 - -1.5 2,750,827 - -3.7 2,750,827 - -2.2 2,750,827 - -2.6 2,750,827 - -1.6 2,750,827 - -2.4 2,750,960 - -1.6 2,750,983 + -0.2 2,750,984 - +2.2 2,751,015 + +1.6 2,751,015 + -0.5 2,751,016 - +3.4 2,751,017 + -1.2 2,751,018 - -1.6 2,751,018 - +0.2 2,751,032 - +1.5 2,751,032 - +0.8 2,751,035 + +0.6 2,751,036 - -0.2 2,751,036 - +0.2 2,751,121 + -3.4 2,751,121 + -1.3 2,751,122 - -1.5 2,751,129 + -0.8 2,751,130 - -1.9 2,751,130 - -1.0 2,751,146 + flhB Sama_2284 0.10 -1.2 2,751,182 + flhB Sama_2284 0.14 -1.1 2,751,182 + flhB Sama_2284 0.14 -2.3 2,751,182 + flhB Sama_2284 0.14 -3.5 2,751,260 + flhB Sama_2284 0.21 -1.2 2,751,323 - flhB Sama_2284 0.26 -1.3 2,751,323 - flhB Sama_2284 0.26 -2.0 2,751,361 - flhB Sama_2284 0.29 -1.5 2,751,361 - flhB Sama_2284 0.29 -2.4 2,751,592 - flhB Sama_2284 0.50 -3.3 2,751,636 + flhB Sama_2284 0.54 -3.2 2,751,756 - flhB Sama_2284 0.64 -1.4 2,751,831 + flhB Sama_2284 0.71 -2.4 2,751,831 + flhB Sama_2284 0.71 -2.6 2,751,831 + flhB Sama_2284 0.71 -0.6 2,751,832 - flhB Sama_2284 0.71 -1.2 2,751,832 - flhB Sama_2284 0.71 -3.0 2,751,832 - flhB Sama_2284 0.71 -2.3 2,751,832 - flhB Sama_2284 0.71 -0.2 2,751,832 - flhB Sama_2284 0.71 +0.8 2,751,921 - flhB Sama_2284 0.79 -2.3 2,751,921 - flhB Sama_2284 0.79 -2.5 2,751,921 - flhB Sama_2284 0.79 -1.3 2,752,130 + -1.2 2,752,146 - -3.0 2,752,282 - fliR Sama_2285 0.15 -2.0 2,752,314 + fliR Sama_2285 0.19 -1.3 2,752,315 - fliR Sama_2285 0.19 -0.2 2,752,315 - fliR Sama_2285 0.19 -0.8 2,752,319 - fliR Sama_2285 0.20 -3.2 2,752,319 - fliR Sama_2285 0.20 -0.5 2,752,319 - fliR Sama_2285 0.20 -1.6 2,752,418 + fliR Sama_2285 0.32 -3.3 2,752,418 + fliR Sama_2285 0.32 -1.5 2,752,419 - fliR Sama_2285 0.33 -3.0 2,752,419 - fliR Sama_2285 0.33 -2.3 2,752,419 - fliR Sama_2285 0.33 -0.8 2,752,419 - fliR Sama_2285 0.33 -1.3 2,752,419 - fliR Sama_2285 0.33 -2.7 2,752,446 - fliR Sama_2285 0.36 -1.2 2,752,446 - fliR Sama_2285 0.36 -1.9 2,752,446 - fliR Sama_2285 0.36 -0.8 2,752,446 - fliR Sama_2285 0.36 -3.1 2,752,450 - fliR Sama_2285 0.37 -1.5 2,752,471 - fliR Sama_2285 0.39 -0.2 2,752,471 - fliR Sama_2285 0.39 +0.8 2,752,485 + fliR Sama_2285 0.41 -2.7 2,752,780 + fliR Sama_2285 0.78 -4.3 2,752,781 - fliR Sama_2285 0.78 -0.8 2,752,845 + fliR Sama_2285 0.87 -2.2 2,752,845 + fliR Sama_2285 0.87 +0.2 2,752,846 - fliR Sama_2285 0.87 -2.5 2,752,846 - fliR Sama_2285 0.87 -3.0 2,752,872 + -1.8 2,752,873 - -0.2 2,752,873 - -0.5 2,752,905 + -1.0 2,752,905 + -0.5 2,752,905 + -1.0 2,752,905 + -0.8 2,752,905 + -0.5 2,752,906 - -3.3 2,752,906 - +0.2 2,752,908 - -0.9 2,752,908 - +0.2 2,752,918 - -2.3 2,752,918 - -2.9 2,752,918 - -1.9 2,752,960 - +0.8 2,753,147 + Sama_2286 0.73 -2.3 2,753,148 - Sama_2286 0.73 -3.2 2,753,148 - Sama_2286 0.73 -2.5 2,753,148 - Sama_2286 0.73 -0.5
Or see this region's nucleotide sequence