Experiment: Motility assay, center cut sample 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phhA and Sama_2223 are separated by 358 nucleotides Sama_2223 and Sama_2224 are separated by 2 nucleotides
Sama_2222: phhA - phenylalanine 4-monooxygenase (RefSeq), at 2,685,807 to 2,686,598
phhA
Sama_2223: Sama_2223 - UTP--glucose-1-phosphate uridylyltransferase (RefSeq), at 2,686,957 to 2,687,865
_2223
Sama_2224: Sama_2224 - UDP-glucose 4-epimerase (RefSeq), at 2,687,868 to 2,688,878
_2224
Position (kb)
2686
2687
2688 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2685.957 kb on - strand, within phhA at 2686.010 kb on + strand, within phhA at 2686.010 kb on + strand, within phhA at 2686.010 kb on + strand, within phhA at 2686.024 kb on + strand, within phhA at 2686.025 kb on - strand, within phhA at 2686.064 kb on - strand, within phhA at 2686.064 kb on - strand, within phhA at 2686.067 kb on + strand, within phhA at 2686.067 kb on + strand, within phhA at 2686.067 kb on + strand, within phhA at 2686.068 kb on - strand, within phhA at 2686.112 kb on + strand, within phhA at 2686.113 kb on - strand, within phhA at 2686.135 kb on + strand, within phhA at 2686.135 kb on + strand, within phhA at 2686.135 kb on + strand, within phhA at 2686.136 kb on - strand, within phhA at 2686.136 kb on - strand, within phhA at 2686.136 kb on - strand, within phhA at 2686.136 kb on - strand, within phhA at 2686.177 kb on + strand, within phhA at 2686.177 kb on + strand, within phhA at 2686.178 kb on - strand, within phhA at 2686.178 kb on - strand, within phhA at 2686.178 kb on - strand, within phhA at 2686.264 kb on + strand, within phhA at 2686.265 kb on - strand, within phhA at 2686.265 kb on - strand, within phhA at 2686.290 kb on - strand, within phhA at 2686.465 kb on + strand, within phhA at 2686.465 kb on + strand, within phhA at 2686.466 kb on - strand, within phhA at 2686.466 kb on - strand, within phhA at 2686.466 kb on - strand, within phhA at 2686.510 kb on + strand, within phhA at 2686.511 kb on - strand, within phhA at 2686.511 kb on - strand, within phhA at 2686.554 kb on + strand at 2686.555 kb on - strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.585 kb on + strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.586 kb on - strand at 2686.592 kb on + strand at 2686.592 kb on + strand at 2686.592 kb on + strand at 2686.634 kb on + strand at 2686.635 kb on - strand at 2686.660 kb on - strand at 2686.675 kb on + strand at 2686.693 kb on + strand at 2686.763 kb on - strand at 2686.875 kb on + strand at 2686.875 kb on + strand at 2686.875 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.890 kb on + strand at 2686.891 kb on - strand at 2686.946 kb on + strand at 2687.112 kb on + strand, within Sama_2223 at 2687.555 kb on + strand, within Sama_2223 at 2687.555 kb on + strand, within Sama_2223 at 2687.556 kb on - strand, within Sama_2223 at 2687.612 kb on + strand, within Sama_2223 at 2687.613 kb on - strand, within Sama_2223 at 2687.772 kb on + strand, within Sama_2223 at 2687.863 kb on + strand at 2687.863 kb on + strand at 2688.249 kb on + strand, within Sama_2224 at 2688.528 kb on + strand, within Sama_2224 at 2688.561 kb on + strand, within Sama_2224 at 2688.639 kb on + strand, within Sama_2224 at 2688.727 kb on + strand, within Sama_2224
Per-strain Table
Position Strand Gene LocusTag Fraction Motility assay, center cut sample 1 remove 2,685,957 - phhA Sama_2222 0.19 +1.1 2,686,010 + phhA Sama_2222 0.26 -2.0 2,686,010 + phhA Sama_2222 0.26 +1.5 2,686,010 + phhA Sama_2222 0.26 +0.8 2,686,024 + phhA Sama_2222 0.27 -1.3 2,686,025 - phhA Sama_2222 0.28 +0.4 2,686,064 - phhA Sama_2222 0.32 -0.5 2,686,064 - phhA Sama_2222 0.32 -0.5 2,686,067 + phhA Sama_2222 0.33 +0.5 2,686,067 + phhA Sama_2222 0.33 +0.2 2,686,067 + phhA Sama_2222 0.33 -2.2 2,686,068 - phhA Sama_2222 0.33 -2.0 2,686,112 + phhA Sama_2222 0.39 +1.6 2,686,113 - phhA Sama_2222 0.39 +0.6 2,686,135 + phhA Sama_2222 0.41 -0.8 2,686,135 + phhA Sama_2222 0.41 +1.2 2,686,135 + phhA Sama_2222 0.41 +2.6 2,686,136 - phhA Sama_2222 0.42 +1.4 2,686,136 - phhA Sama_2222 0.42 -0.5 2,686,136 - phhA Sama_2222 0.42 -0.7 2,686,136 - phhA Sama_2222 0.42 +0.4 2,686,177 + phhA Sama_2222 0.47 -0.2 2,686,177 + phhA Sama_2222 0.47 +0.8 2,686,178 - phhA Sama_2222 0.47 -0.1 2,686,178 - phhA Sama_2222 0.47 -0.2 2,686,178 - phhA Sama_2222 0.47 -2.0 2,686,264 + phhA Sama_2222 0.58 +0.2 2,686,265 - phhA Sama_2222 0.58 -1.2 2,686,265 - phhA Sama_2222 0.58 -1.0 2,686,290 - phhA Sama_2222 0.61 -0.2 2,686,465 + phhA Sama_2222 0.83 -0.2 2,686,465 + phhA Sama_2222 0.83 -1.3 2,686,466 - phhA Sama_2222 0.83 +0.2 2,686,466 - phhA Sama_2222 0.83 -0.1 2,686,466 - phhA Sama_2222 0.83 +0.7 2,686,510 + phhA Sama_2222 0.89 -0.0 2,686,511 - phhA Sama_2222 0.89 -0.2 2,686,511 - phhA Sama_2222 0.89 +0.2 2,686,554 + +0.4 2,686,555 - -1.5 2,686,585 + +0.4 2,686,585 + -0.2 2,686,585 + -2.1 2,686,585 + -2.1 2,686,585 + +0.0 2,686,585 + +1.4 2,686,586 - +2.4 2,686,586 - -0.6 2,686,586 - -2.5 2,686,586 - +0.5 2,686,586 - +0.1 2,686,586 - -1.0 2,686,586 - +1.6 2,686,586 - +2.3 2,686,586 - -1.8 2,686,586 - -0.9 2,686,592 + -0.1 2,686,592 + -1.0 2,686,592 + +0.6 2,686,634 + +1.6 2,686,635 - -0.8 2,686,660 - +1.2 2,686,675 + -1.0 2,686,693 + +0.2 2,686,763 - -1.5 2,686,875 + -0.8 2,686,875 + +2.3 2,686,875 + -0.8 2,686,890 + -1.6 2,686,890 + -0.5 2,686,890 + -0.8 2,686,890 + -0.8 2,686,890 + +0.8 2,686,890 + -1.4 2,686,890 + -2.3 2,686,890 + -0.2 2,686,890 + +1.9 2,686,891 - -0.4 2,686,946 + -1.0 2,687,112 + Sama_2223 0.17 -1.6 2,687,555 + Sama_2223 0.66 +0.8 2,687,555 + Sama_2223 0.66 -2.0 2,687,556 - Sama_2223 0.66 -0.8 2,687,612 + Sama_2223 0.72 -3.0 2,687,613 - Sama_2223 0.72 -1.9 2,687,772 + Sama_2223 0.90 -2.0 2,687,863 + -0.5 2,687,863 + -0.5 2,688,249 + Sama_2224 0.38 -2.6 2,688,528 + Sama_2224 0.65 -0.8 2,688,561 + Sama_2224 0.69 -2.3 2,688,639 + Sama_2224 0.76 -2.1 2,688,727 + Sama_2224 0.85 -2.9
Or see this region's nucleotide sequence