Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgI and flgH are separated by 12 nucleotides flgH and flgG are separated by 12 nucleotides flgG and flgF are separated by 12 nucleotides flgF and flgE are separated by 166 nucleotides
Sama_2311: flgI - flagellar basal body P-ring protein (RefSeq), at 2,777,392 to 2,778,483
flgI
Sama_2312: flgH - flagellar basal body L-ring protein (RefSeq), at 2,778,496 to 2,779,170
flgH
Sama_2313: flgG - flagellar basal body rod protein FlgG (RefSeq), at 2,779,183 to 2,779,971
flgG
Sama_2314: flgF - flagellar basal body rod protein FlgF (RefSeq), at 2,779,984 to 2,780,727
flgF
Sama_2315: flgE - flagellar hook protein FlgE (RefSeq), at 2,780,894 to 2,782,255
flgE
Position (kb)
2779
2780 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2778.260 kb on - strand, within flgI at 2778.277 kb on - strand, within flgI at 2778.304 kb on - strand, within flgI at 2778.304 kb on - strand, within flgI at 2778.321 kb on - strand, within flgI at 2778.414 kb on + strand at 2778.415 kb on - strand at 2778.578 kb on + strand, within flgH at 2778.579 kb on - strand, within flgH at 2778.610 kb on + strand, within flgH at 2778.610 kb on + strand, within flgH at 2778.610 kb on + strand, within flgH at 2778.611 kb on - strand, within flgH at 2778.611 kb on - strand, within flgH at 2778.611 kb on - strand, within flgH at 2778.611 kb on - strand, within flgH at 2778.756 kb on + strand, within flgH at 2778.756 kb on + strand, within flgH at 2778.756 kb on + strand, within flgH at 2778.757 kb on - strand, within flgH at 2778.757 kb on - strand, within flgH at 2778.846 kb on - strand, within flgH at 2779.039 kb on - strand, within flgH at 2779.039 kb on - strand, within flgH at 2779.039 kb on - strand, within flgH at 2779.082 kb on + strand, within flgH at 2779.082 kb on + strand, within flgH at 2779.086 kb on - strand, within flgH at 2779.102 kb on - strand, within flgH at 2779.102 kb on - strand, within flgH at 2779.102 kb on - strand, within flgH at 2779.116 kb on + strand at 2779.117 kb on - strand at 2779.155 kb on + strand at 2779.155 kb on + strand at 2779.156 kb on - strand at 2779.156 kb on - strand at 2779.156 kb on - strand at 2779.160 kb on + strand at 2779.160 kb on + strand at 2779.170 kb on + strand at 2779.184 kb on + strand at 2779.202 kb on + strand at 2779.202 kb on + strand at 2779.202 kb on + strand at 2779.250 kb on + strand at 2779.251 kb on - strand at 2779.251 kb on - strand at 2779.251 kb on - strand at 2779.251 kb on - strand at 2779.408 kb on + strand, within flgG at 2779.408 kb on + strand, within flgG at 2779.409 kb on - strand, within flgG at 2779.410 kb on + strand, within flgG at 2779.434 kb on + strand, within flgG at 2779.492 kb on + strand, within flgG at 2779.493 kb on - strand, within flgG at 2779.493 kb on - strand, within flgG at 2779.493 kb on - strand, within flgG at 2779.524 kb on + strand, within flgG at 2779.524 kb on + strand, within flgG at 2779.525 kb on - strand, within flgG at 2779.568 kb on + strand, within flgG at 2779.568 kb on + strand, within flgG at 2779.568 kb on + strand, within flgG at 2779.568 kb on + strand, within flgG at 2779.568 kb on + strand, within flgG at 2779.568 kb on + strand, within flgG at 2779.569 kb on - strand, within flgG at 2779.569 kb on - strand, within flgG at 2779.588 kb on + strand, within flgG at 2779.629 kb on + strand, within flgG at 2779.631 kb on + strand, within flgG at 2779.632 kb on - strand, within flgG at 2779.632 kb on - strand, within flgG at 2779.728 kb on + strand, within flgG at 2779.768 kb on + strand, within flgG at 2779.769 kb on - strand, within flgG at 2779.769 kb on - strand, within flgG at 2779.770 kb on + strand, within flgG at 2779.770 kb on + strand, within flgG at 2779.770 kb on + strand, within flgG at 2779.771 kb on - strand, within flgG at 2779.835 kb on + strand, within flgG at 2779.841 kb on - strand, within flgG at 2779.841 kb on - strand, within flgG at 2779.841 kb on - strand, within flgG at 2779.841 kb on - strand, within flgG at 2779.841 kb on - strand, within flgG at 2779.937 kb on - strand at 2779.937 kb on - strand at 2779.937 kb on - strand at 2779.947 kb on + strand at 2779.948 kb on - strand at 2779.948 kb on - strand at 2779.951 kb on + strand at 2779.951 kb on + strand at 2779.952 kb on - strand at 2779.952 kb on - strand at 2779.957 kb on + strand at 2779.958 kb on - strand at 2779.971 kb on + strand at 2779.972 kb on - strand at 2779.972 kb on - strand at 2779.972 kb on - strand at 2779.992 kb on + strand at 2779.992 kb on + strand at 2779.993 kb on - strand at 2779.996 kb on + strand at 2779.997 kb on - strand at 2780.048 kb on - strand at 2780.048 kb on - strand at 2780.112 kb on + strand, within flgF at 2780.112 kb on + strand, within flgF at 2780.272 kb on - strand, within flgF at 2780.295 kb on + strand, within flgF at 2780.295 kb on + strand, within flgF at 2780.295 kb on + strand, within flgF at 2780.432 kb on + strand, within flgF at 2780.432 kb on + strand, within flgF at 2780.432 kb on + strand, within flgF at 2780.432 kb on + strand, within flgF at 2780.433 kb on - strand, within flgF at 2780.433 kb on - strand, within flgF at 2780.514 kb on + strand, within flgF at 2780.709 kb on + strand at 2780.711 kb on + strand at 2780.712 kb on - strand at 2780.712 kb on - strand at 2780.748 kb on + strand at 2780.748 kb on + strand at 2780.748 kb on + strand at 2780.749 kb on - strand at 2780.823 kb on + strand at 2780.823 kb on + strand at 2780.823 kb on + strand at 2780.823 kb on + strand at 2780.823 kb on + strand at 2780.823 kb on + strand at 2780.823 kb on + strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.824 kb on - strand at 2780.833 kb on - strand at 2780.844 kb on - strand at 2780.844 kb on - strand at 2780.930 kb on - strand at 2780.945 kb on - strand at 2780.945 kb on - strand at 2780.965 kb on + strand at 2780.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 2,778,260 - flgI Sama_2311 0.79 -0.1 2,778,277 - flgI Sama_2311 0.81 -2.9 2,778,304 - flgI Sama_2311 0.84 -1.4 2,778,304 - flgI Sama_2311 0.84 -0.3 2,778,321 - flgI Sama_2311 0.85 -1.1 2,778,414 + -2.5 2,778,415 - -2.1 2,778,578 + flgH Sama_2312 0.12 +0.3 2,778,579 - flgH Sama_2312 0.12 -1.5 2,778,610 + flgH Sama_2312 0.17 -0.7 2,778,610 + flgH Sama_2312 0.17 -1.7 2,778,610 + flgH Sama_2312 0.17 -0.9 2,778,611 - flgH Sama_2312 0.17 -2.1 2,778,611 - flgH Sama_2312 0.17 -2.6 2,778,611 - flgH Sama_2312 0.17 -1.0 2,778,611 - flgH Sama_2312 0.17 -1.9 2,778,756 + flgH Sama_2312 0.39 -1.6 2,778,756 + flgH Sama_2312 0.39 +0.4 2,778,756 + flgH Sama_2312 0.39 +1.4 2,778,757 - flgH Sama_2312 0.39 +0.4 2,778,757 - flgH Sama_2312 0.39 -0.6 2,778,846 - flgH Sama_2312 0.52 -0.7 2,779,039 - flgH Sama_2312 0.80 -1.3 2,779,039 - flgH Sama_2312 0.80 -3.1 2,779,039 - flgH Sama_2312 0.80 +0.6 2,779,082 + flgH Sama_2312 0.87 -2.1 2,779,082 + flgH Sama_2312 0.87 -0.7 2,779,086 - flgH Sama_2312 0.87 -1.3 2,779,102 - flgH Sama_2312 0.90 -0.1 2,779,102 - flgH Sama_2312 0.90 -0.1 2,779,102 - flgH Sama_2312 0.90 -1.3 2,779,116 + -1.0 2,779,117 - -0.8 2,779,155 + -1.2 2,779,155 + -0.7 2,779,156 - -0.9 2,779,156 - +0.1 2,779,156 - +0.1 2,779,160 + -0.9 2,779,160 + -0.1 2,779,170 + -0.3 2,779,184 + +0.6 2,779,202 + -0.6 2,779,202 + -1.3 2,779,202 + -0.6 2,779,250 + -1.4 2,779,251 - -0.9 2,779,251 - -1.0 2,779,251 - +0.0 2,779,251 - -1.1 2,779,408 + flgG Sama_2313 0.29 +0.4 2,779,408 + flgG Sama_2313 0.29 -1.1 2,779,409 - flgG Sama_2313 0.29 -1.6 2,779,410 + flgG Sama_2313 0.29 -1.4 2,779,434 + flgG Sama_2313 0.32 -1.3 2,779,492 + flgG Sama_2313 0.39 -0.9 2,779,493 - flgG Sama_2313 0.39 -1.5 2,779,493 - flgG Sama_2313 0.39 +0.4 2,779,493 - flgG Sama_2313 0.39 -1.1 2,779,524 + flgG Sama_2313 0.43 -0.4 2,779,524 + flgG Sama_2313 0.43 -0.2 2,779,525 - flgG Sama_2313 0.43 -1.0 2,779,568 + flgG Sama_2313 0.49 -0.6 2,779,568 + flgG Sama_2313 0.49 -0.9 2,779,568 + flgG Sama_2313 0.49 -0.7 2,779,568 + flgG Sama_2313 0.49 -3.0 2,779,568 + flgG Sama_2313 0.49 -1.0 2,779,568 + flgG Sama_2313 0.49 -0.6 2,779,569 - flgG Sama_2313 0.49 -1.2 2,779,569 - flgG Sama_2313 0.49 -0.8 2,779,588 + flgG Sama_2313 0.51 +1.4 2,779,629 + flgG Sama_2313 0.57 -2.0 2,779,631 + flgG Sama_2313 0.57 -0.9 2,779,632 - flgG Sama_2313 0.57 +0.6 2,779,632 - flgG Sama_2313 0.57 +0.3 2,779,728 + flgG Sama_2313 0.69 -1.0 2,779,768 + flgG Sama_2313 0.74 -1.0 2,779,769 - flgG Sama_2313 0.74 -2.4 2,779,769 - flgG Sama_2313 0.74 -0.1 2,779,770 + flgG Sama_2313 0.74 -2.1 2,779,770 + flgG Sama_2313 0.74 -1.1 2,779,770 + flgG Sama_2313 0.74 -0.0 2,779,771 - flgG Sama_2313 0.75 -0.6 2,779,835 + flgG Sama_2313 0.83 +0.0 2,779,841 - flgG Sama_2313 0.83 -1.5 2,779,841 - flgG Sama_2313 0.83 -0.4 2,779,841 - flgG Sama_2313 0.83 -1.7 2,779,841 - flgG Sama_2313 0.83 -0.6 2,779,841 - flgG Sama_2313 0.83 -0.6 2,779,937 - -1.8 2,779,937 - -0.6 2,779,937 - -1.5 2,779,947 + -1.1 2,779,948 - +0.9 2,779,948 - -0.6 2,779,951 + -3.1 2,779,951 + -0.3 2,779,952 - -1.4 2,779,952 - -1.4 2,779,957 + -0.9 2,779,958 - -1.4 2,779,971 + +0.2 2,779,972 - -1.4 2,779,972 - -0.6 2,779,972 - -1.1 2,779,992 + -0.7 2,779,992 + +0.3 2,779,993 - +0.4 2,779,996 + -0.9 2,779,997 - -0.6 2,780,048 - -1.5 2,780,048 - -1.0 2,780,112 + flgF Sama_2314 0.17 +0.0 2,780,112 + flgF Sama_2314 0.17 -0.6 2,780,272 - flgF Sama_2314 0.39 +0.1 2,780,295 + flgF Sama_2314 0.42 -1.8 2,780,295 + flgF Sama_2314 0.42 -0.9 2,780,295 + flgF Sama_2314 0.42 -1.0 2,780,432 + flgF Sama_2314 0.60 -0.3 2,780,432 + flgF Sama_2314 0.60 -1.4 2,780,432 + flgF Sama_2314 0.60 -1.1 2,780,432 + flgF Sama_2314 0.60 -3.6 2,780,433 - flgF Sama_2314 0.60 +0.9 2,780,433 - flgF Sama_2314 0.60 -0.6 2,780,514 + flgF Sama_2314 0.71 -0.3 2,780,709 + -0.7 2,780,711 + -0.9 2,780,712 - -3.0 2,780,712 - -0.7 2,780,748 + +1.4 2,780,748 + -0.9 2,780,748 + -0.1 2,780,749 - -0.1 2,780,823 + -0.1 2,780,823 + +0.1 2,780,823 + -0.0 2,780,823 + -1.3 2,780,823 + +0.5 2,780,823 + -0.3 2,780,823 + +1.4 2,780,824 - +0.6 2,780,824 - +0.4 2,780,824 - +0.7 2,780,824 - +0.2 2,780,824 - +0.2 2,780,824 - +0.0 2,780,824 - +0.2 2,780,824 - +0.9 2,780,833 - +0.1 2,780,844 - -2.4 2,780,844 - +0.9 2,780,930 - -1.4 2,780,945 - -0.7 2,780,945 - -0.2 2,780,965 + -0.7 2,780,966 - -0.9
Or see this region's nucleotide sequence