Strain Fitness in Shewanella amazonensis SB2B around Sama_2312

Experiment: Spectinomycin 0.0125 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgI and flgH are separated by 12 nucleotidesflgH and flgG are separated by 12 nucleotidesflgG and flgF are separated by 12 nucleotides Sama_2311: flgI - flagellar basal body P-ring protein (RefSeq), at 2,777,392 to 2,778,483 flgI Sama_2312: flgH - flagellar basal body L-ring protein (RefSeq), at 2,778,496 to 2,779,170 flgH Sama_2313: flgG - flagellar basal body rod protein FlgG (RefSeq), at 2,779,183 to 2,779,971 flgG Sama_2314: flgF - flagellar basal body rod protein FlgF (RefSeq), at 2,779,984 to 2,780,727 flgF Position (kb) 2778 2779 2780Strain fitness (log2 ratio) -3 -2 -1 0 1at 2777.535 kb on + strand, within flgIat 2777.535 kb on + strand, within flgIat 2777.535 kb on + strand, within flgIat 2777.535 kb on + strand, within flgIat 2777.535 kb on + strand, within flgIat 2777.535 kb on + strand, within flgIat 2777.535 kb on + strand, within flgIat 2777.536 kb on - strand, within flgIat 2777.536 kb on - strand, within flgIat 2777.536 kb on - strand, within flgIat 2777.536 kb on - strand, within flgIat 2777.536 kb on - strand, within flgIat 2777.536 kb on - strand, within flgIat 2777.536 kb on - strand, within flgIat 2777.536 kb on - strand, within flgIat 2777.541 kb on + strand, within flgIat 2777.560 kb on + strand, within flgIat 2777.560 kb on + strand, within flgIat 2777.560 kb on + strand, within flgIat 2777.560 kb on + strand, within flgIat 2777.560 kb on + strand, within flgIat 2777.560 kb on + strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.561 kb on - strand, within flgIat 2777.674 kb on + strand, within flgIat 2777.677 kb on + strand, within flgIat 2777.678 kb on - strand, within flgIat 2777.678 kb on - strand, within flgIat 2777.678 kb on - strand, within flgIat 2777.701 kb on + strand, within flgIat 2777.702 kb on - strand, within flgIat 2777.702 kb on - strand, within flgIat 2777.816 kb on + strand, within flgIat 2777.816 kb on + strand, within flgIat 2777.816 kb on + strand, within flgIat 2777.816 kb on + strand, within flgIat 2777.816 kb on + strand, within flgIat 2777.816 kb on + strand, within flgIat 2777.817 kb on - strand, within flgIat 2777.817 kb on - strand, within flgIat 2777.817 kb on - strand, within flgIat 2777.817 kb on - strand, within flgIat 2777.817 kb on - strand, within flgIat 2777.931 kb on + strand, within flgIat 2777.932 kb on - strand, within flgIat 2778.012 kb on + strand, within flgIat 2778.021 kb on + strand, within flgIat 2778.021 kb on + strand, within flgIat 2778.090 kb on - strand, within flgIat 2778.112 kb on - strand, within flgIat 2778.160 kb on + strand, within flgIat 2778.260 kb on - strand, within flgIat 2778.277 kb on - strand, within flgIat 2778.304 kb on - strand, within flgIat 2778.304 kb on - strand, within flgIat 2778.321 kb on - strand, within flgIat 2778.414 kb on + strandat 2778.415 kb on - strandat 2778.578 kb on + strand, within flgHat 2778.579 kb on - strand, within flgHat 2778.610 kb on + strand, within flgHat 2778.610 kb on + strand, within flgHat 2778.610 kb on + strand, within flgHat 2778.611 kb on - strand, within flgHat 2778.611 kb on - strand, within flgHat 2778.611 kb on - strand, within flgHat 2778.611 kb on - strand, within flgHat 2778.756 kb on + strand, within flgHat 2778.756 kb on + strand, within flgHat 2778.756 kb on + strand, within flgHat 2778.757 kb on - strand, within flgHat 2778.757 kb on - strand, within flgHat 2778.846 kb on - strand, within flgHat 2779.039 kb on - strand, within flgHat 2779.039 kb on - strand, within flgHat 2779.039 kb on - strand, within flgHat 2779.082 kb on + strand, within flgHat 2779.082 kb on + strand, within flgHat 2779.086 kb on - strand, within flgHat 2779.102 kb on - strand, within flgHat 2779.102 kb on - strand, within flgHat 2779.102 kb on - strand, within flgHat 2779.116 kb on + strandat 2779.117 kb on - strandat 2779.155 kb on + strandat 2779.155 kb on + strandat 2779.156 kb on - strandat 2779.156 kb on - strandat 2779.156 kb on - strandat 2779.160 kb on + strandat 2779.160 kb on + strandat 2779.170 kb on + strandat 2779.184 kb on + strandat 2779.202 kb on + strandat 2779.202 kb on + strandat 2779.202 kb on + strandat 2779.250 kb on + strandat 2779.251 kb on - strandat 2779.251 kb on - strandat 2779.251 kb on - strandat 2779.251 kb on - strandat 2779.408 kb on + strand, within flgGat 2779.408 kb on + strand, within flgGat 2779.409 kb on - strand, within flgGat 2779.410 kb on + strand, within flgGat 2779.434 kb on + strand, within flgGat 2779.492 kb on + strand, within flgGat 2779.493 kb on - strand, within flgGat 2779.493 kb on - strand, within flgGat 2779.493 kb on - strand, within flgGat 2779.524 kb on + strand, within flgGat 2779.524 kb on + strand, within flgGat 2779.525 kb on - strand, within flgGat 2779.568 kb on + strand, within flgGat 2779.568 kb on + strand, within flgGat 2779.568 kb on + strand, within flgGat 2779.568 kb on + strand, within flgGat 2779.568 kb on + strand, within flgGat 2779.568 kb on + strand, within flgGat 2779.569 kb on - strand, within flgGat 2779.569 kb on - strand, within flgGat 2779.588 kb on + strand, within flgGat 2779.629 kb on + strand, within flgGat 2779.631 kb on + strand, within flgGat 2779.632 kb on - strand, within flgGat 2779.632 kb on - strand, within flgGat 2779.728 kb on + strand, within flgGat 2779.768 kb on + strand, within flgGat 2779.769 kb on - strand, within flgGat 2779.769 kb on - strand, within flgGat 2779.770 kb on + strand, within flgGat 2779.770 kb on + strand, within flgGat 2779.770 kb on + strand, within flgGat 2779.771 kb on - strand, within flgGat 2779.835 kb on + strand, within flgGat 2779.841 kb on - strand, within flgGat 2779.841 kb on - strand, within flgGat 2779.841 kb on - strand, within flgGat 2779.841 kb on - strand, within flgGat 2779.841 kb on - strand, within flgGat 2779.937 kb on - strandat 2779.937 kb on - strandat 2779.937 kb on - strandat 2779.947 kb on + strandat 2779.948 kb on - strandat 2779.948 kb on - strandat 2779.951 kb on + strandat 2779.951 kb on + strandat 2779.952 kb on - strandat 2779.952 kb on - strandat 2779.957 kb on + strandat 2779.958 kb on - strandat 2779.971 kb on + strandat 2779.972 kb on - strandat 2779.972 kb on - strandat 2779.972 kb on - strandat 2779.992 kb on + strandat 2779.992 kb on + strandat 2779.993 kb on - strandat 2779.996 kb on + strandat 2779.997 kb on - strandat 2780.048 kb on - strandat 2780.048 kb on - strandat 2780.112 kb on + strand, within flgFat 2780.112 kb on + strand, within flgF

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml
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2,777,535 + flgI Sama_2311 0.13 -2.6
2,777,535 + flgI Sama_2311 0.13 -2.7
2,777,535 + flgI Sama_2311 0.13 -1.6
2,777,535 + flgI Sama_2311 0.13 -0.6
2,777,535 + flgI Sama_2311 0.13 -0.1
2,777,535 + flgI Sama_2311 0.13 +0.7
2,777,535 + flgI Sama_2311 0.13 +0.4
2,777,536 - flgI Sama_2311 0.13 -1.3
2,777,536 - flgI Sama_2311 0.13 -1.3
2,777,536 - flgI Sama_2311 0.13 -1.1
2,777,536 - flgI Sama_2311 0.13 -2.7
2,777,536 - flgI Sama_2311 0.13 -0.1
2,777,536 - flgI Sama_2311 0.13 -0.5
2,777,536 - flgI Sama_2311 0.13 -0.6
2,777,536 - flgI Sama_2311 0.13 +0.8
2,777,541 + flgI Sama_2311 0.14 -1.4
2,777,560 + flgI Sama_2311 0.15 +0.4
2,777,560 + flgI Sama_2311 0.15 -0.6
2,777,560 + flgI Sama_2311 0.15 -1.4
2,777,560 + flgI Sama_2311 0.15 -1.9
2,777,560 + flgI Sama_2311 0.15 +1.0
2,777,560 + flgI Sama_2311 0.15 -1.6
2,777,561 - flgI Sama_2311 0.15 -1.1
2,777,561 - flgI Sama_2311 0.15 -0.6
2,777,561 - flgI Sama_2311 0.15 -0.1
2,777,561 - flgI Sama_2311 0.15 +0.1
2,777,561 - flgI Sama_2311 0.15 -1.4
2,777,561 - flgI Sama_2311 0.15 -1.9
2,777,561 - flgI Sama_2311 0.15 +0.2
2,777,561 - flgI Sama_2311 0.15 -1.1
2,777,561 - flgI Sama_2311 0.15 -1.1
2,777,561 - flgI Sama_2311 0.15 -0.1
2,777,561 - flgI Sama_2311 0.15 -1.5
2,777,561 - flgI Sama_2311 0.15 +0.8
2,777,674 + flgI Sama_2311 0.26 -1.8
2,777,677 + flgI Sama_2311 0.26 -1.1
2,777,678 - flgI Sama_2311 0.26 -0.4
2,777,678 - flgI Sama_2311 0.26 -1.6
2,777,678 - flgI Sama_2311 0.26 -0.3
2,777,701 + flgI Sama_2311 0.28 -0.1
2,777,702 - flgI Sama_2311 0.28 +0.2
2,777,702 - flgI Sama_2311 0.28 -1.0
2,777,816 + flgI Sama_2311 0.39 -0.9
2,777,816 + flgI Sama_2311 0.39 -1.9
2,777,816 + flgI Sama_2311 0.39 -0.9
2,777,816 + flgI Sama_2311 0.39 -0.0
2,777,816 + flgI Sama_2311 0.39 -0.7
2,777,816 + flgI Sama_2311 0.39 -1.8
2,777,817 - flgI Sama_2311 0.39 -0.1
2,777,817 - flgI Sama_2311 0.39 -1.1
2,777,817 - flgI Sama_2311 0.39 -1.6
2,777,817 - flgI Sama_2311 0.39 -0.6
2,777,817 - flgI Sama_2311 0.39 -0.8
2,777,931 + flgI Sama_2311 0.49 -0.6
2,777,932 - flgI Sama_2311 0.49 -1.4
2,778,012 + flgI Sama_2311 0.57 -1.1
2,778,021 + flgI Sama_2311 0.58 -1.7
2,778,021 + flgI Sama_2311 0.58 -0.5
2,778,090 - flgI Sama_2311 0.64 -1.0
2,778,112 - flgI Sama_2311 0.66 -0.6
2,778,160 + flgI Sama_2311 0.70 +0.2
2,778,260 - flgI Sama_2311 0.79 -0.1
2,778,277 - flgI Sama_2311 0.81 -2.9
2,778,304 - flgI Sama_2311 0.84 -1.4
2,778,304 - flgI Sama_2311 0.84 -0.3
2,778,321 - flgI Sama_2311 0.85 -1.1
2,778,414 + -2.5
2,778,415 - -2.1
2,778,578 + flgH Sama_2312 0.12 +0.3
2,778,579 - flgH Sama_2312 0.12 -1.5
2,778,610 + flgH Sama_2312 0.17 -0.7
2,778,610 + flgH Sama_2312 0.17 -1.7
2,778,610 + flgH Sama_2312 0.17 -0.9
2,778,611 - flgH Sama_2312 0.17 -2.1
2,778,611 - flgH Sama_2312 0.17 -2.6
2,778,611 - flgH Sama_2312 0.17 -1.0
2,778,611 - flgH Sama_2312 0.17 -1.9
2,778,756 + flgH Sama_2312 0.39 -1.6
2,778,756 + flgH Sama_2312 0.39 +0.4
2,778,756 + flgH Sama_2312 0.39 +1.4
2,778,757 - flgH Sama_2312 0.39 +0.4
2,778,757 - flgH Sama_2312 0.39 -0.6
2,778,846 - flgH Sama_2312 0.52 -0.7
2,779,039 - flgH Sama_2312 0.80 -1.3
2,779,039 - flgH Sama_2312 0.80 -3.1
2,779,039 - flgH Sama_2312 0.80 +0.6
2,779,082 + flgH Sama_2312 0.87 -2.1
2,779,082 + flgH Sama_2312 0.87 -0.7
2,779,086 - flgH Sama_2312 0.87 -1.3
2,779,102 - flgH Sama_2312 0.90 -0.1
2,779,102 - flgH Sama_2312 0.90 -0.1
2,779,102 - flgH Sama_2312 0.90 -1.3
2,779,116 + -1.0
2,779,117 - -0.8
2,779,155 + -1.2
2,779,155 + -0.7
2,779,156 - -0.9
2,779,156 - +0.1
2,779,156 - +0.1
2,779,160 + -0.9
2,779,160 + -0.1
2,779,170 + -0.3
2,779,184 + +0.6
2,779,202 + -0.6
2,779,202 + -1.3
2,779,202 + -0.6
2,779,250 + -1.4
2,779,251 - -0.9
2,779,251 - -1.0
2,779,251 - +0.0
2,779,251 - -1.1
2,779,408 + flgG Sama_2313 0.29 +0.4
2,779,408 + flgG Sama_2313 0.29 -1.1
2,779,409 - flgG Sama_2313 0.29 -1.6
2,779,410 + flgG Sama_2313 0.29 -1.4
2,779,434 + flgG Sama_2313 0.32 -1.3
2,779,492 + flgG Sama_2313 0.39 -0.9
2,779,493 - flgG Sama_2313 0.39 -1.5
2,779,493 - flgG Sama_2313 0.39 +0.4
2,779,493 - flgG Sama_2313 0.39 -1.1
2,779,524 + flgG Sama_2313 0.43 -0.4
2,779,524 + flgG Sama_2313 0.43 -0.2
2,779,525 - flgG Sama_2313 0.43 -1.0
2,779,568 + flgG Sama_2313 0.49 -0.6
2,779,568 + flgG Sama_2313 0.49 -0.9
2,779,568 + flgG Sama_2313 0.49 -0.7
2,779,568 + flgG Sama_2313 0.49 -3.0
2,779,568 + flgG Sama_2313 0.49 -1.0
2,779,568 + flgG Sama_2313 0.49 -0.6
2,779,569 - flgG Sama_2313 0.49 -1.2
2,779,569 - flgG Sama_2313 0.49 -0.8
2,779,588 + flgG Sama_2313 0.51 +1.4
2,779,629 + flgG Sama_2313 0.57 -2.0
2,779,631 + flgG Sama_2313 0.57 -0.9
2,779,632 - flgG Sama_2313 0.57 +0.6
2,779,632 - flgG Sama_2313 0.57 +0.3
2,779,728 + flgG Sama_2313 0.69 -1.0
2,779,768 + flgG Sama_2313 0.74 -1.0
2,779,769 - flgG Sama_2313 0.74 -2.4
2,779,769 - flgG Sama_2313 0.74 -0.1
2,779,770 + flgG Sama_2313 0.74 -2.1
2,779,770 + flgG Sama_2313 0.74 -1.1
2,779,770 + flgG Sama_2313 0.74 -0.0
2,779,771 - flgG Sama_2313 0.75 -0.6
2,779,835 + flgG Sama_2313 0.83 +0.0
2,779,841 - flgG Sama_2313 0.83 -1.5
2,779,841 - flgG Sama_2313 0.83 -0.4
2,779,841 - flgG Sama_2313 0.83 -1.7
2,779,841 - flgG Sama_2313 0.83 -0.6
2,779,841 - flgG Sama_2313 0.83 -0.6
2,779,937 - -1.8
2,779,937 - -0.6
2,779,937 - -1.5
2,779,947 + -1.1
2,779,948 - +0.9
2,779,948 - -0.6
2,779,951 + -3.1
2,779,951 + -0.3
2,779,952 - -1.4
2,779,952 - -1.4
2,779,957 + -0.9
2,779,958 - -1.4
2,779,971 + +0.2
2,779,972 - -1.4
2,779,972 - -0.6
2,779,972 - -1.1
2,779,992 + -0.7
2,779,992 + +0.3
2,779,993 - +0.4
2,779,996 + -0.9
2,779,997 - -0.6
2,780,048 - -1.5
2,780,048 - -1.0
2,780,112 + flgF Sama_2314 0.17 +0.0
2,780,112 + flgF Sama_2314 0.17 -0.6

Or see this region's nucleotide sequence