Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliG and fliF overlap by 8 nucleotides fliF and Sama_2298 are separated by 24 nucleotides Sama_2298 and Sama_2299 are separated by 123 nucleotides
Sama_2296: fliG - flagellar motor switch protein G (RefSeq), at 2,760,988 to 2,762,025
fliG
Sama_2297: fliF - flagellar MS-ring protein (RefSeq), at 2,762,018 to 2,763,715
fliF
Sama_2298: Sama_2298 - flagellar hook-basal body complex protein (FliE) (RefSeq), at 2,763,740 to 2,764,075
_2298
Sama_2299: Sama_2299 - response regulator receiver protein (RefSeq), at 2,764,199 to 2,765,539
_2299
Position (kb)
2762
2763
2764 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2761.155 kb on + strand, within fliG at 2761.283 kb on - strand, within fliG at 2761.373 kb on + strand, within fliG at 2761.374 kb on - strand, within fliG at 2761.374 kb on - strand, within fliG at 2761.490 kb on - strand, within fliG at 2761.490 kb on - strand, within fliG at 2761.490 kb on - strand, within fliG at 2761.584 kb on - strand, within fliG at 2761.915 kb on - strand, within fliG at 2762.102 kb on - strand at 2762.108 kb on - strand at 2762.108 kb on - strand at 2762.158 kb on - strand at 2762.158 kb on - strand at 2762.158 kb on - strand at 2762.164 kb on - strand at 2762.164 kb on - strand at 2762.387 kb on - strand, within fliF at 2762.387 kb on - strand, within fliF at 2762.387 kb on - strand, within fliF at 2762.387 kb on - strand, within fliF at 2762.387 kb on - strand, within fliF at 2762.387 kb on - strand, within fliF at 2762.387 kb on - strand, within fliF at 2762.445 kb on - strand, within fliF at 2762.445 kb on - strand, within fliF at 2762.445 kb on - strand, within fliF at 2762.445 kb on - strand, within fliF at 2762.445 kb on - strand, within fliF at 2762.445 kb on - strand, within fliF at 2762.570 kb on - strand, within fliF at 2762.570 kb on - strand, within fliF at 2762.589 kb on - strand, within fliF at 2762.589 kb on - strand, within fliF at 2762.589 kb on - strand, within fliF at 2762.589 kb on - strand, within fliF at 2762.589 kb on - strand, within fliF at 2762.653 kb on - strand, within fliF at 2762.653 kb on - strand, within fliF at 2762.657 kb on - strand, within fliF at 2763.087 kb on - strand, within fliF at 2763.102 kb on - strand, within fliF at 2763.102 kb on - strand, within fliF at 2763.102 kb on - strand, within fliF at 2763.104 kb on - strand, within fliF at 2763.386 kb on - strand, within fliF at 2763.415 kb on - strand, within fliF at 2763.415 kb on - strand, within fliF at 2763.415 kb on - strand, within fliF at 2763.419 kb on - strand, within fliF at 2763.419 kb on - strand, within fliF at 2763.457 kb on - strand, within fliF at 2763.518 kb on - strand, within fliF at 2763.668 kb on + strand at 2763.669 kb on - strand at 2763.703 kb on + strand at 2763.704 kb on - strand at 2763.839 kb on + strand, within Sama_2298 at 2763.840 kb on - strand, within Sama_2298 at 2763.845 kb on - strand, within Sama_2298 at 2763.930 kb on - strand, within Sama_2298 at 2763.930 kb on - strand, within Sama_2298 at 2764.009 kb on + strand, within Sama_2298 at 2764.010 kb on - strand, within Sama_2298 at 2764.010 kb on - strand, within Sama_2298 at 2764.010 kb on - strand, within Sama_2298 at 2764.010 kb on - strand, within Sama_2298 at 2764.010 kb on - strand, within Sama_2298 at 2764.068 kb on - strand at 2764.068 kb on - strand at 2764.068 kb on - strand at 2764.068 kb on - strand at 2764.068 kb on - strand at 2764.075 kb on + strand at 2764.109 kb on - strand at 2764.166 kb on - strand at 2764.166 kb on - strand at 2764.166 kb on - strand at 2764.197 kb on + strand at 2764.197 kb on + strand at 2764.198 kb on - strand at 2764.198 kb on - strand at 2764.208 kb on + strand at 2764.209 kb on - strand at 2764.216 kb on + strand at 2764.217 kb on - strand at 2764.245 kb on + strand at 2764.246 kb on - strand at 2764.246 kb on - strand at 2764.329 kb on - strand at 2764.329 kb on - strand at 2764.430 kb on - strand, within Sama_2299 at 2764.448 kb on + strand, within Sama_2299 at 2764.449 kb on - strand, within Sama_2299 at 2764.449 kb on - strand, within Sama_2299 at 2764.533 kb on - strand, within Sama_2299 at 2764.667 kb on + strand, within Sama_2299 at 2764.668 kb on - strand, within Sama_2299
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 2,761,155 + fliG Sama_2296 0.16 -1.2 2,761,283 - fliG Sama_2296 0.28 -1.1 2,761,373 + fliG Sama_2296 0.37 -1.2 2,761,374 - fliG Sama_2296 0.37 -1.7 2,761,374 - fliG Sama_2296 0.37 -0.6 2,761,490 - fliG Sama_2296 0.48 -1.6 2,761,490 - fliG Sama_2296 0.48 -1.8 2,761,490 - fliG Sama_2296 0.48 +0.0 2,761,584 - fliG Sama_2296 0.57 -0.2 2,761,915 - fliG Sama_2296 0.89 -1.7 2,762,102 - -1.3 2,762,108 - -0.2 2,762,108 - -1.2 2,762,158 - -2.7 2,762,158 - -1.4 2,762,158 - -0.4 2,762,164 - -1.9 2,762,164 - -1.4 2,762,387 - fliF Sama_2297 0.22 -0.4 2,762,387 - fliF Sama_2297 0.22 +0.1 2,762,387 - fliF Sama_2297 0.22 -2.6 2,762,387 - fliF Sama_2297 0.22 -1.7 2,762,387 - fliF Sama_2297 0.22 -1.2 2,762,387 - fliF Sama_2297 0.22 +0.4 2,762,387 - fliF Sama_2297 0.22 -1.6 2,762,445 - fliF Sama_2297 0.25 -0.3 2,762,445 - fliF Sama_2297 0.25 +0.0 2,762,445 - fliF Sama_2297 0.25 -0.5 2,762,445 - fliF Sama_2297 0.25 -1.4 2,762,445 - fliF Sama_2297 0.25 -2.2 2,762,445 - fliF Sama_2297 0.25 -0.2 2,762,570 - fliF Sama_2297 0.33 -1.2 2,762,570 - fliF Sama_2297 0.33 -0.9 2,762,589 - fliF Sama_2297 0.34 -0.1 2,762,589 - fliF Sama_2297 0.34 -0.9 2,762,589 - fliF Sama_2297 0.34 -0.9 2,762,589 - fliF Sama_2297 0.34 +0.5 2,762,589 - fliF Sama_2297 0.34 -2.0 2,762,653 - fliF Sama_2297 0.37 -1.4 2,762,653 - fliF Sama_2297 0.37 -0.3 2,762,657 - fliF Sama_2297 0.38 -2.2 2,763,087 - fliF Sama_2297 0.63 -2.2 2,763,102 - fliF Sama_2297 0.64 -0.6 2,763,102 - fliF Sama_2297 0.64 -0.4 2,763,102 - fliF Sama_2297 0.64 -1.0 2,763,104 - fliF Sama_2297 0.64 -1.5 2,763,386 - fliF Sama_2297 0.81 -1.2 2,763,415 - fliF Sama_2297 0.82 -1.0 2,763,415 - fliF Sama_2297 0.82 -1.4 2,763,415 - fliF Sama_2297 0.82 +0.1 2,763,419 - fliF Sama_2297 0.83 -0.5 2,763,419 - fliF Sama_2297 0.83 -2.2 2,763,457 - fliF Sama_2297 0.85 +0.0 2,763,518 - fliF Sama_2297 0.88 -2.1 2,763,668 + -0.8 2,763,669 - -0.8 2,763,703 + -0.6 2,763,704 - -0.3 2,763,839 + Sama_2298 0.29 -0.3 2,763,840 - Sama_2298 0.30 -1.4 2,763,845 - Sama_2298 0.31 -1.3 2,763,930 - Sama_2298 0.57 -1.9 2,763,930 - Sama_2298 0.57 -1.8 2,764,009 + Sama_2298 0.80 -0.2 2,764,010 - Sama_2298 0.80 -1.9 2,764,010 - Sama_2298 0.80 +0.7 2,764,010 - Sama_2298 0.80 -0.6 2,764,010 - Sama_2298 0.80 -0.8 2,764,010 - Sama_2298 0.80 -0.9 2,764,068 - -0.9 2,764,068 - +0.2 2,764,068 - -0.6 2,764,068 - -2.6 2,764,068 - -0.9 2,764,075 + -3.0 2,764,109 - +0.1 2,764,166 - -0.4 2,764,166 - +0.8 2,764,166 - -1.9 2,764,197 + -1.7 2,764,197 + +0.7 2,764,198 - -0.2 2,764,198 - +0.6 2,764,208 + -0.9 2,764,209 - +0.7 2,764,216 + +0.2 2,764,217 - -0.2 2,764,245 + +0.0 2,764,246 - +0.8 2,764,246 - -0.8 2,764,329 - +1.0 2,764,329 - -0.2 2,764,430 - Sama_2299 0.17 +1.0 2,764,448 + Sama_2299 0.19 +0.0 2,764,449 - Sama_2299 0.19 +1.0 2,764,449 - Sama_2299 0.19 +0.2 2,764,533 - Sama_2299 0.25 -0.7 2,764,667 + Sama_2299 0.35 +0.6 2,764,668 - Sama_2299 0.35 +0.2
Or see this region's nucleotide sequence