Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and Sama_2288 overlap by 4 nucleotides Sama_2288 and fliN are separated by 17 nucleotides fliN and fliM are separated by 11 nucleotides fliM and fliL are separated by 38 nucleotides fliL and Sama_2292 are separated by 85 nucleotides
Sama_2287: fliP - flagellar biosynthesis protein FliP (RefSeq), at 2,753,225 to 2,753,974
fliP
Sama_2288: Sama_2288 - flagellar protein FliO (RefSeq), at 2,753,971 to 2,754,345
_2288
Sama_2289: fliN - flagellar motor switch protein (RefSeq), at 2,754,363 to 2,754,737
fliN
Sama_2290: fliM - flagellar motor switch protein FliM (RefSeq), at 2,754,749 to 2,755,777
fliM
Sama_2291: fliL - flagellar basal body-associated protein FliL (RefSeq), at 2,755,816 to 2,756,343
fliL
Sama_2292: Sama_2292 - flagellar hook-length control protein FliK (RefSeq), at 2,756,429 to 2,758,210
_2292
Position (kb)
2754
2755
2756 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2753.759 kb on + strand, within fliP at 2753.759 kb on + strand, within fliP at 2753.760 kb on - strand, within fliP at 2753.760 kb on - strand, within fliP at 2753.788 kb on + strand, within fliP at 2753.961 kb on - strand at 2754.048 kb on + strand, within Sama_2288 at 2754.048 kb on + strand, within Sama_2288 at 2754.052 kb on + strand, within Sama_2288 at 2754.052 kb on + strand, within Sama_2288 at 2754.052 kb on + strand, within Sama_2288 at 2754.052 kb on + strand, within Sama_2288 at 2754.053 kb on - strand, within Sama_2288 at 2754.053 kb on - strand, within Sama_2288 at 2754.053 kb on - strand, within Sama_2288 at 2754.053 kb on - strand, within Sama_2288 at 2754.053 kb on - strand, within Sama_2288 at 2754.053 kb on - strand, within Sama_2288 at 2754.089 kb on + strand, within Sama_2288 at 2754.089 kb on + strand, within Sama_2288 at 2754.090 kb on - strand, within Sama_2288 at 2754.206 kb on + strand, within Sama_2288 at 2754.206 kb on + strand, within Sama_2288 at 2754.206 kb on + strand, within Sama_2288 at 2754.206 kb on + strand, within Sama_2288 at 2754.207 kb on - strand, within Sama_2288 at 2754.207 kb on - strand, within Sama_2288 at 2754.207 kb on - strand, within Sama_2288 at 2754.207 kb on - strand, within Sama_2288 at 2754.207 kb on - strand, within Sama_2288 at 2754.207 kb on - strand, within Sama_2288 at 2754.236 kb on - strand, within Sama_2288 at 2754.331 kb on + strand at 2754.338 kb on - strand at 2754.365 kb on - strand at 2754.464 kb on + strand, within fliN at 2754.558 kb on - strand, within fliN at 2754.558 kb on - strand, within fliN at 2754.558 kb on - strand, within fliN at 2754.559 kb on + strand, within fliN at 2754.559 kb on + strand, within fliN at 2754.560 kb on - strand, within fliN at 2754.560 kb on - strand, within fliN at 2754.560 kb on - strand, within fliN at 2754.560 kb on - strand, within fliN at 2754.560 kb on - strand, within fliN at 2754.560 kb on - strand, within fliN at 2754.560 kb on - strand, within fliN at 2754.578 kb on + strand, within fliN at 2754.579 kb on - strand, within fliN at 2754.732 kb on - strand at 2754.743 kb on - strand at 2754.764 kb on - strand at 2754.764 kb on - strand at 2754.764 kb on - strand at 2754.764 kb on - strand at 2754.798 kb on - strand at 2754.840 kb on - strand at 2754.886 kb on - strand, within fliM at 2754.918 kb on + strand, within fliM at 2754.918 kb on + strand, within fliM at 2754.919 kb on - strand, within fliM at 2754.981 kb on - strand, within fliM at 2755.040 kb on - strand, within fliM at 2755.040 kb on - strand, within fliM at 2755.076 kb on - strand, within fliM at 2755.129 kb on - strand, within fliM at 2755.196 kb on + strand, within fliM at 2755.205 kb on - strand, within fliM at 2755.291 kb on + strand, within fliM at 2755.292 kb on - strand, within fliM at 2755.294 kb on - strand, within fliM at 2755.414 kb on + strand, within fliM at 2755.414 kb on + strand, within fliM at 2755.442 kb on - strand, within fliM at 2755.477 kb on + strand, within fliM at 2755.505 kb on - strand, within fliM at 2755.512 kb on + strand, within fliM at 2755.513 kb on - strand, within fliM at 2755.513 kb on - strand, within fliM at 2755.513 kb on - strand, within fliM at 2755.544 kb on - strand, within fliM at 2755.573 kb on + strand, within fliM at 2755.586 kb on + strand, within fliM at 2755.587 kb on - strand, within fliM at 2755.587 kb on - strand, within fliM at 2755.587 kb on - strand, within fliM at 2755.587 kb on - strand, within fliM at 2755.762 kb on + strand at 2755.766 kb on + strand at 2755.766 kb on + strand at 2755.766 kb on - strand at 2755.800 kb on - strand at 2755.805 kb on + strand at 2755.806 kb on - strand at 2755.806 kb on - strand at 2755.806 kb on - strand at 2755.818 kb on - strand at 2755.818 kb on - strand at 2755.885 kb on - strand, within fliL at 2755.885 kb on - strand, within fliL at 2755.885 kb on - strand, within fliL at 2755.888 kb on - strand, within fliL at 2755.892 kb on - strand, within fliL at 2755.892 kb on - strand, within fliL at 2755.892 kb on - strand, within fliL at 2755.916 kb on - strand, within fliL at 2755.916 kb on - strand, within fliL at 2755.916 kb on - strand, within fliL at 2756.014 kb on - strand, within fliL at 2756.014 kb on - strand, within fliL at 2756.124 kb on - strand, within fliL at 2756.124 kb on - strand, within fliL at 2756.124 kb on - strand, within fliL at 2756.249 kb on - strand, within fliL at 2756.293 kb on - strand at 2756.293 kb on - strand at 2756.293 kb on - strand at 2756.293 kb on - strand at 2756.311 kb on - strand at 2756.324 kb on - strand at 2756.324 kb on - strand at 2756.404 kb on - strand at 2756.404 kb on - strand at 2756.404 kb on - strand at 2756.404 kb on - strand at 2756.416 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.418 kb on - strand at 2756.423 kb on - strand at 2756.423 kb on - strand at 2756.431 kb on - strand at 2756.437 kb on - strand at 2756.437 kb on - strand at 2756.437 kb on - strand at 2756.437 kb on - strand at 2756.440 kb on - strand at 2756.440 kb on - strand at 2756.440 kb on - strand at 2756.445 kb on - strand at 2756.445 kb on - strand at 2756.445 kb on - strand at 2756.580 kb on - strand at 2756.652 kb on - strand, within Sama_2292 at 2756.652 kb on - strand, within Sama_2292 at 2756.708 kb on + strand, within Sama_2292 at 2756.771 kb on + strand, within Sama_2292 at 2756.771 kb on + strand, within Sama_2292 at 2756.772 kb on - strand, within Sama_2292
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 2,753,759 + fliP Sama_2287 0.71 -2.2 2,753,759 + fliP Sama_2287 0.71 -0.1 2,753,760 - fliP Sama_2287 0.71 -0.9 2,753,760 - fliP Sama_2287 0.71 -0.0 2,753,788 + fliP Sama_2287 0.75 -2.0 2,753,961 - -0.6 2,754,048 + Sama_2288 0.21 -1.3 2,754,048 + Sama_2288 0.21 -2.6 2,754,052 + Sama_2288 0.22 -0.6 2,754,052 + Sama_2288 0.22 +1.0 2,754,052 + Sama_2288 0.22 -0.8 2,754,052 + Sama_2288 0.22 +1.4 2,754,053 - Sama_2288 0.22 +0.0 2,754,053 - Sama_2288 0.22 -1.4 2,754,053 - Sama_2288 0.22 -1.1 2,754,053 - Sama_2288 0.22 -1.8 2,754,053 - Sama_2288 0.22 -0.2 2,754,053 - Sama_2288 0.22 -1.2 2,754,089 + Sama_2288 0.31 +0.8 2,754,089 + Sama_2288 0.31 -2.3 2,754,090 - Sama_2288 0.32 -0.9 2,754,206 + Sama_2288 0.63 +0.2 2,754,206 + Sama_2288 0.63 +1.1 2,754,206 + Sama_2288 0.63 -0.6 2,754,206 + Sama_2288 0.63 -0.9 2,754,207 - Sama_2288 0.63 -1.6 2,754,207 - Sama_2288 0.63 +0.2 2,754,207 - Sama_2288 0.63 +0.2 2,754,207 - Sama_2288 0.63 -0.1 2,754,207 - Sama_2288 0.63 -1.2 2,754,207 - Sama_2288 0.63 -1.1 2,754,236 - Sama_2288 0.71 -0.4 2,754,331 + -0.8 2,754,338 - +0.9 2,754,365 - +0.4 2,754,464 + fliN Sama_2289 0.27 -2.4 2,754,558 - fliN Sama_2289 0.52 +0.4 2,754,558 - fliN Sama_2289 0.52 +0.1 2,754,558 - fliN Sama_2289 0.52 +2.0 2,754,559 + fliN Sama_2289 0.52 -0.3 2,754,559 + fliN Sama_2289 0.52 -1.3 2,754,560 - fliN Sama_2289 0.53 -1.1 2,754,560 - fliN Sama_2289 0.53 -2.6 2,754,560 - fliN Sama_2289 0.53 -0.9 2,754,560 - fliN Sama_2289 0.53 -1.4 2,754,560 - fliN Sama_2289 0.53 -1.6 2,754,560 - fliN Sama_2289 0.53 -1.4 2,754,560 - fliN Sama_2289 0.53 -0.6 2,754,578 + fliN Sama_2289 0.57 -0.9 2,754,579 - fliN Sama_2289 0.58 -0.8 2,754,732 - -2.4 2,754,743 - +0.1 2,754,764 - -0.8 2,754,764 - +0.1 2,754,764 - +0.2 2,754,764 - -0.4 2,754,798 - -1.2 2,754,840 - -2.7 2,754,886 - fliM Sama_2290 0.13 -1.5 2,754,918 + fliM Sama_2290 0.16 -1.3 2,754,918 + fliM Sama_2290 0.16 -0.6 2,754,919 - fliM Sama_2290 0.17 +0.1 2,754,981 - fliM Sama_2290 0.23 -1.2 2,755,040 - fliM Sama_2290 0.28 -1.9 2,755,040 - fliM Sama_2290 0.28 +0.4 2,755,076 - fliM Sama_2290 0.32 -1.9 2,755,129 - fliM Sama_2290 0.37 -1.7 2,755,196 + fliM Sama_2290 0.43 -0.2 2,755,205 - fliM Sama_2290 0.44 -1.3 2,755,291 + fliM Sama_2290 0.53 +0.2 2,755,292 - fliM Sama_2290 0.53 -2.0 2,755,294 - fliM Sama_2290 0.53 +0.2 2,755,414 + fliM Sama_2290 0.65 -0.6 2,755,414 + fliM Sama_2290 0.65 -3.6 2,755,442 - fliM Sama_2290 0.67 +1.0 2,755,477 + fliM Sama_2290 0.71 -0.2 2,755,505 - fliM Sama_2290 0.73 -0.4 2,755,512 + fliM Sama_2290 0.74 -0.0 2,755,513 - fliM Sama_2290 0.74 -1.9 2,755,513 - fliM Sama_2290 0.74 -0.4 2,755,513 - fliM Sama_2290 0.74 -2.2 2,755,544 - fliM Sama_2290 0.77 -1.9 2,755,573 + fliM Sama_2290 0.80 -2.2 2,755,586 + fliM Sama_2290 0.81 -3.2 2,755,587 - fliM Sama_2290 0.81 -0.9 2,755,587 - fliM Sama_2290 0.81 -0.6 2,755,587 - fliM Sama_2290 0.81 -0.6 2,755,587 - fliM Sama_2290 0.81 -1.4 2,755,762 + -2.1 2,755,766 + -2.2 2,755,766 + -0.6 2,755,766 - +0.4 2,755,800 - -0.3 2,755,805 + -2.3 2,755,806 - -0.6 2,755,806 - -0.6 2,755,806 - -0.0 2,755,818 - +1.1 2,755,818 - -1.6 2,755,885 - fliL Sama_2291 0.13 -1.0 2,755,885 - fliL Sama_2291 0.13 -0.2 2,755,885 - fliL Sama_2291 0.13 -0.6 2,755,888 - fliL Sama_2291 0.14 -0.9 2,755,892 - fliL Sama_2291 0.14 +0.3 2,755,892 - fliL Sama_2291 0.14 +0.4 2,755,892 - fliL Sama_2291 0.14 -0.5 2,755,916 - fliL Sama_2291 0.19 +0.8 2,755,916 - fliL Sama_2291 0.19 +0.4 2,755,916 - fliL Sama_2291 0.19 -1.0 2,756,014 - fliL Sama_2291 0.38 -0.4 2,756,014 - fliL Sama_2291 0.38 -0.6 2,756,124 - fliL Sama_2291 0.58 +0.2 2,756,124 - fliL Sama_2291 0.58 +0.8 2,756,124 - fliL Sama_2291 0.58 -0.3 2,756,249 - fliL Sama_2291 0.82 +0.1 2,756,293 - +0.0 2,756,293 - +1.8 2,756,293 - +1.2 2,756,293 - +1.0 2,756,311 - +0.0 2,756,324 - -0.1 2,756,324 - -0.4 2,756,404 - +0.2 2,756,404 - +0.1 2,756,404 - -0.2 2,756,404 - +0.4 2,756,416 - -0.3 2,756,418 - +0.2 2,756,418 - -0.9 2,756,418 - +0.4 2,756,418 - -0.3 2,756,418 - +0.2 2,756,418 - +0.1 2,756,423 - +1.2 2,756,423 - -1.0 2,756,431 - -0.5 2,756,437 - -0.3 2,756,437 - -0.4 2,756,437 - -0.1 2,756,437 - -0.6 2,756,440 - -0.2 2,756,440 - -0.3 2,756,440 - -1.4 2,756,445 - -0.3 2,756,445 - -1.2 2,756,445 - -2.3 2,756,580 - -0.4 2,756,652 - Sama_2292 0.13 -1.2 2,756,652 - Sama_2292 0.13 -1.2 2,756,708 + Sama_2292 0.16 +1.3 2,756,771 + Sama_2292 0.19 -0.8 2,756,771 + Sama_2292 0.19 -0.9 2,756,772 - Sama_2292 0.19 -2.4
Or see this region's nucleotide sequence