Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliA and Sama_2281 overlap by 8 nucleotides Sama_2281 and flhF are separated by 4 nucleotides flhF and flhA are separated by 12 nucleotides
Sama_2280: fliA - flagellar biosynthesis sigma factor (RefSeq), at 2,745,826 to 2,746,545
fliA
Sama_2281: Sama_2281 - cobyrinic acid a,c-diamide synthase (RefSeq), at 2,746,538 to 2,747,419
_2281
Sama_2282: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 2,747,424 to 2,748,815
flhF
Sama_2283: flhA - flagellar biosynthesis protein FlhA (RefSeq), at 2,748,828 to 2,750,927
flhA
Position (kb)
2747
2748
2749 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2746.430 kb on - strand, within fliA at 2746.430 kb on - strand, within fliA at 2746.455 kb on + strand, within fliA at 2746.455 kb on + strand, within fliA at 2746.456 kb on - strand, within fliA at 2746.456 kb on - strand, within fliA at 2746.456 kb on - strand, within fliA at 2746.456 kb on - strand, within fliA at 2746.456 kb on - strand, within fliA at 2746.487 kb on + strand at 2746.509 kb on + strand at 2746.510 kb on - strand at 2746.525 kb on - strand at 2746.525 kb on - strand at 2746.526 kb on + strand at 2746.527 kb on - strand at 2746.527 kb on - strand at 2746.540 kb on - strand at 2746.540 kb on - strand at 2746.578 kb on - strand at 2746.578 kb on - strand at 2746.628 kb on - strand, within Sama_2281 at 2746.646 kb on - strand, within Sama_2281 at 2746.646 kb on - strand, within Sama_2281 at 2746.663 kb on - strand, within Sama_2281 at 2746.698 kb on - strand, within Sama_2281 at 2746.698 kb on - strand, within Sama_2281 at 2746.715 kb on - strand, within Sama_2281 at 2746.719 kb on - strand, within Sama_2281 at 2746.838 kb on - strand, within Sama_2281 at 2746.847 kb on - strand, within Sama_2281 at 2746.847 kb on - strand, within Sama_2281 at 2746.847 kb on - strand, within Sama_2281 at 2746.941 kb on - strand, within Sama_2281 at 2746.941 kb on - strand, within Sama_2281 at 2746.941 kb on - strand, within Sama_2281 at 2746.941 kb on - strand, within Sama_2281 at 2746.941 kb on - strand, within Sama_2281 at 2746.941 kb on - strand, within Sama_2281 at 2746.944 kb on - strand, within Sama_2281 at 2746.944 kb on - strand, within Sama_2281 at 2746.944 kb on - strand, within Sama_2281 at 2747.018 kb on - strand, within Sama_2281 at 2747.114 kb on - strand, within Sama_2281 at 2747.124 kb on - strand, within Sama_2281 at 2747.124 kb on - strand, within Sama_2281 at 2747.184 kb on - strand, within Sama_2281 at 2747.300 kb on - strand, within Sama_2281 at 2747.374 kb on - strand at 2747.390 kb on - strand at 2747.420 kb on - strand at 2747.426 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.460 kb on - strand at 2747.544 kb on - strand at 2747.605 kb on - strand, within flhF at 2747.605 kb on - strand, within flhF at 2747.653 kb on - strand, within flhF at 2747.653 kb on - strand, within flhF at 2747.827 kb on - strand, within flhF at 2747.827 kb on - strand, within flhF at 2747.827 kb on - strand, within flhF at 2747.827 kb on - strand, within flhF at 2747.827 kb on - strand, within flhF at 2747.827 kb on - strand, within flhF at 2747.827 kb on - strand, within flhF at 2747.827 kb on - strand, within flhF at 2747.833 kb on - strand, within flhF at 2747.836 kb on - strand, within flhF at 2747.836 kb on - strand, within flhF at 2747.836 kb on - strand, within flhF at 2747.836 kb on - strand, within flhF at 2747.836 kb on - strand, within flhF at 2747.836 kb on - strand, within flhF at 2748.030 kb on - strand, within flhF at 2748.088 kb on - strand, within flhF at 2748.088 kb on - strand, within flhF at 2748.093 kb on - strand, within flhF at 2748.164 kb on - strand, within flhF at 2748.164 kb on - strand, within flhF at 2748.164 kb on - strand, within flhF at 2748.304 kb on - strand, within flhF at 2748.393 kb on - strand, within flhF at 2748.579 kb on - strand, within flhF at 2748.579 kb on - strand, within flhF at 2748.612 kb on - strand, within flhF at 2748.622 kb on - strand, within flhF at 2748.622 kb on - strand, within flhF at 2748.622 kb on - strand, within flhF at 2748.786 kb on - strand at 2748.964 kb on - strand at 2749.117 kb on - strand, within flhA at 2749.117 kb on - strand, within flhA at 2749.169 kb on + strand, within flhA at 2749.170 kb on - strand, within flhA at 2749.178 kb on + strand, within flhA at 2749.197 kb on + strand, within flhA at 2749.202 kb on - strand, within flhA at 2749.225 kb on + strand, within flhA at 2749.225 kb on + strand, within flhA at 2749.226 kb on - strand, within flhA at 2749.226 kb on - strand, within flhA at 2749.226 kb on - strand, within flhA at 2749.246 kb on - strand, within flhA at 2749.246 kb on - strand, within flhA at 2749.246 kb on - strand, within flhA at 2749.246 kb on - strand, within flhA at 2749.280 kb on + strand, within flhA at 2749.281 kb on - strand, within flhA at 2749.389 kb on + strand, within flhA at 2749.390 kb on - strand, within flhA at 2749.390 kb on - strand, within flhA at 2749.390 kb on - strand, within flhA at 2749.399 kb on + strand, within flhA at 2749.400 kb on - strand, within flhA at 2749.422 kb on - strand, within flhA at 2749.422 kb on - strand, within flhA at 2749.561 kb on - strand, within flhA at 2749.641 kb on + strand, within flhA at 2749.746 kb on + strand, within flhA at 2749.746 kb on + strand, within flhA at 2749.746 kb on + strand, within flhA at 2749.746 kb on + strand, within flhA at 2749.747 kb on - strand, within flhA at 2749.747 kb on - strand, within flhA at 2749.747 kb on - strand, within flhA at 2749.747 kb on - strand, within flhA at 2749.747 kb on - strand, within flhA at 2749.747 kb on - strand, within flhA at 2749.747 kb on - strand, within flhA at 2749.747 kb on - strand, within flhA
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 2,746,430 - fliA Sama_2280 0.84 -0.4 2,746,430 - fliA Sama_2280 0.84 -0.8 2,746,455 + fliA Sama_2280 0.87 -2.6 2,746,455 + fliA Sama_2280 0.87 -0.4 2,746,456 - fliA Sama_2280 0.88 +1.2 2,746,456 - fliA Sama_2280 0.88 -0.4 2,746,456 - fliA Sama_2280 0.88 -1.7 2,746,456 - fliA Sama_2280 0.88 -0.7 2,746,456 - fliA Sama_2280 0.88 -1.5 2,746,487 + +0.4 2,746,509 + -0.7 2,746,510 - -2.1 2,746,525 - -0.1 2,746,525 - -0.8 2,746,526 + -0.4 2,746,527 - -0.9 2,746,527 - -1.2 2,746,540 - -0.1 2,746,540 - -0.3 2,746,578 - -0.9 2,746,578 - -1.2 2,746,628 - Sama_2281 0.10 -0.6 2,746,646 - Sama_2281 0.12 -0.7 2,746,646 - Sama_2281 0.12 -1.3 2,746,663 - Sama_2281 0.14 -1.2 2,746,698 - Sama_2281 0.18 -1.2 2,746,698 - Sama_2281 0.18 -2.6 2,746,715 - Sama_2281 0.20 -0.3 2,746,719 - Sama_2281 0.21 -1.2 2,746,838 - Sama_2281 0.34 -1.4 2,746,847 - Sama_2281 0.35 -1.7 2,746,847 - Sama_2281 0.35 -0.9 2,746,847 - Sama_2281 0.35 -1.2 2,746,941 - Sama_2281 0.46 -1.6 2,746,941 - Sama_2281 0.46 -0.6 2,746,941 - Sama_2281 0.46 -1.0 2,746,941 - Sama_2281 0.46 -2.7 2,746,941 - Sama_2281 0.46 -2.2 2,746,941 - Sama_2281 0.46 -1.2 2,746,944 - Sama_2281 0.46 -2.0 2,746,944 - Sama_2281 0.46 -2.0 2,746,944 - Sama_2281 0.46 -2.9 2,747,018 - Sama_2281 0.54 -1.6 2,747,114 - Sama_2281 0.65 +0.4 2,747,124 - Sama_2281 0.66 -1.1 2,747,124 - Sama_2281 0.66 -3.5 2,747,184 - Sama_2281 0.73 -1.9 2,747,300 - Sama_2281 0.86 -2.1 2,747,374 - +0.4 2,747,390 - +1.0 2,747,420 - -1.5 2,747,426 - -0.2 2,747,460 - +1.8 2,747,460 - -0.8 2,747,460 - -1.2 2,747,460 - -0.3 2,747,460 - -0.6 2,747,460 - -0.2 2,747,460 - -0.3 2,747,460 - +0.0 2,747,460 - +0.6 2,747,460 - -1.3 2,747,460 - -0.6 2,747,544 - -0.7 2,747,605 - flhF Sama_2282 0.13 -1.3 2,747,605 - flhF Sama_2282 0.13 -0.5 2,747,653 - flhF Sama_2282 0.16 -0.3 2,747,653 - flhF Sama_2282 0.16 -0.8 2,747,827 - flhF Sama_2282 0.29 +0.0 2,747,827 - flhF Sama_2282 0.29 -0.7 2,747,827 - flhF Sama_2282 0.29 -1.3 2,747,827 - flhF Sama_2282 0.29 -1.4 2,747,827 - flhF Sama_2282 0.29 -0.6 2,747,827 - flhF Sama_2282 0.29 -2.0 2,747,827 - flhF Sama_2282 0.29 +0.2 2,747,827 - flhF Sama_2282 0.29 +0.1 2,747,833 - flhF Sama_2282 0.29 -0.3 2,747,836 - flhF Sama_2282 0.30 -0.6 2,747,836 - flhF Sama_2282 0.30 -1.5 2,747,836 - flhF Sama_2282 0.30 -1.3 2,747,836 - flhF Sama_2282 0.30 -1.6 2,747,836 - flhF Sama_2282 0.30 -2.2 2,747,836 - flhF Sama_2282 0.30 -1.2 2,748,030 - flhF Sama_2282 0.44 -1.3 2,748,088 - flhF Sama_2282 0.48 -2.5 2,748,088 - flhF Sama_2282 0.48 -2.6 2,748,093 - flhF Sama_2282 0.48 -1.6 2,748,164 - flhF Sama_2282 0.53 -0.1 2,748,164 - flhF Sama_2282 0.53 -0.9 2,748,164 - flhF Sama_2282 0.53 -0.6 2,748,304 - flhF Sama_2282 0.63 -2.5 2,748,393 - flhF Sama_2282 0.70 -0.8 2,748,579 - flhF Sama_2282 0.83 -1.7 2,748,579 - flhF Sama_2282 0.83 -0.6 2,748,612 - flhF Sama_2282 0.85 -2.4 2,748,622 - flhF Sama_2282 0.86 -0.9 2,748,622 - flhF Sama_2282 0.86 -1.2 2,748,622 - flhF Sama_2282 0.86 -0.6 2,748,786 - -2.9 2,748,964 - -1.2 2,749,117 - flhA Sama_2283 0.14 -2.3 2,749,117 - flhA Sama_2283 0.14 -1.2 2,749,169 + flhA Sama_2283 0.16 -0.1 2,749,170 - flhA Sama_2283 0.16 +0.1 2,749,178 + flhA Sama_2283 0.17 -1.8 2,749,197 + flhA Sama_2283 0.18 -2.7 2,749,202 - flhA Sama_2283 0.18 +0.2 2,749,225 + flhA Sama_2283 0.19 -2.2 2,749,225 + flhA Sama_2283 0.19 -1.9 2,749,226 - flhA Sama_2283 0.19 -1.8 2,749,226 - flhA Sama_2283 0.19 -1.0 2,749,226 - flhA Sama_2283 0.19 -1.3 2,749,246 - flhA Sama_2283 0.20 -1.2 2,749,246 - flhA Sama_2283 0.20 -0.9 2,749,246 - flhA Sama_2283 0.20 -2.2 2,749,246 - flhA Sama_2283 0.20 -2.2 2,749,280 + flhA Sama_2283 0.22 -1.6 2,749,281 - flhA Sama_2283 0.22 -0.8 2,749,389 + flhA Sama_2283 0.27 -1.9 2,749,390 - flhA Sama_2283 0.27 -1.2 2,749,390 - flhA Sama_2283 0.27 +0.7 2,749,390 - flhA Sama_2283 0.27 -0.1 2,749,399 + flhA Sama_2283 0.27 -0.7 2,749,400 - flhA Sama_2283 0.27 -0.9 2,749,422 - flhA Sama_2283 0.28 -3.0 2,749,422 - flhA Sama_2283 0.28 -1.6 2,749,561 - flhA Sama_2283 0.35 -0.4 2,749,641 + flhA Sama_2283 0.39 -2.9 2,749,746 + flhA Sama_2283 0.44 +1.0 2,749,746 + flhA Sama_2283 0.44 +0.1 2,749,746 + flhA Sama_2283 0.44 -1.7 2,749,746 + flhA Sama_2283 0.44 -1.0 2,749,747 - flhA Sama_2283 0.44 -1.9 2,749,747 - flhA Sama_2283 0.44 -0.7 2,749,747 - flhA Sama_2283 0.44 -1.9 2,749,747 - flhA Sama_2283 0.44 +0.3 2,749,747 - flhA Sama_2283 0.44 -0.7 2,749,747 - flhA Sama_2283 0.44 -1.6 2,749,747 - flhA Sama_2283 0.44 -2.1 2,749,747 - flhA Sama_2283 0.44 -1.6
Or see this region's nucleotide sequence