Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1908 and Sama_1909 are separated by 172 nucleotides Sama_1909 and bcp are separated by 26 nucleotides bcp and Sama_1911 are separated by 99 nucleotides
Sama_1908: Sama_1908 - dihydrodipicolinate synthase (RefSeq), at 2,336,495 to 2,337,376
_1908
Sama_1909: Sama_1909 - glycine cleavage system transcriptional repressor, putative (RefSeq), at 2,337,549 to 2,338,076
_1909
Sama_1910: bcp - thioredoxin-dependent thiol peroxidase (RefSeq), at 2,338,103 to 2,338,567
bcp
Sama_1911: Sama_1911 - hypothetical protein (RefSeq), at 2,338,667 to 2,339,785
_1911
Position (kb)
2337
2338
2339 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2337.411 kb on - strand at 2337.441 kb on + strand at 2337.441 kb on + strand at 2337.442 kb on - strand at 2337.442 kb on - strand at 2337.536 kb on - strand at 2337.536 kb on - strand at 2337.546 kb on + strand at 2337.546 kb on + strand at 2337.546 kb on + strand at 2337.546 kb on + strand at 2337.547 kb on - strand at 2337.547 kb on - strand at 2337.681 kb on - strand, within Sama_1909 at 2337.810 kb on + strand, within Sama_1909 at 2337.810 kb on + strand, within Sama_1909 at 2337.811 kb on - strand, within Sama_1909 at 2337.811 kb on - strand, within Sama_1909 at 2337.811 kb on - strand, within Sama_1909 at 2338.016 kb on + strand, within Sama_1909 at 2338.017 kb on - strand, within Sama_1909 at 2338.017 kb on - strand, within Sama_1909 at 2338.058 kb on + strand at 2338.059 kb on - strand at 2338.059 kb on - strand at 2338.101 kb on + strand at 2338.114 kb on - strand at 2338.367 kb on - strand, within bcp at 2338.367 kb on - strand, within bcp at 2338.444 kb on + strand, within bcp at 2338.449 kb on + strand, within bcp at 2338.449 kb on + strand, within bcp at 2338.449 kb on + strand, within bcp at 2338.451 kb on + strand, within bcp at 2338.451 kb on + strand, within bcp at 2338.452 kb on - strand, within bcp at 2338.638 kb on - strand at 2338.669 kb on - strand at 2338.669 kb on - strand at 2338.736 kb on - strand at 2338.736 kb on - strand at 2338.736 kb on - strand at 2339.022 kb on + strand, within Sama_1911 at 2339.023 kb on - strand, within Sama_1911 at 2339.030 kb on - strand, within Sama_1911 at 2339.030 kb on - strand, within Sama_1911
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 2,337,411 - -0.8 2,337,441 + +0.6 2,337,441 + +0.4 2,337,442 - -0.5 2,337,442 - +0.1 2,337,536 - -1.0 2,337,536 - -2.1 2,337,546 + -1.4 2,337,546 + +0.5 2,337,546 + -1.1 2,337,546 + -0.8 2,337,547 - -1.8 2,337,547 - -2.8 2,337,681 - Sama_1909 0.25 -1.5 2,337,810 + Sama_1909 0.49 -1.7 2,337,810 + Sama_1909 0.49 -1.1 2,337,811 - Sama_1909 0.50 -2.1 2,337,811 - Sama_1909 0.50 -2.5 2,337,811 - Sama_1909 0.50 -1.5 2,338,016 + Sama_1909 0.88 +0.2 2,338,017 - Sama_1909 0.89 -2.9 2,338,017 - Sama_1909 0.89 +0.2 2,338,058 + +0.4 2,338,059 - -0.1 2,338,059 - -0.4 2,338,101 + -0.0 2,338,114 - -0.2 2,338,367 - bcp Sama_1910 0.57 -1.1 2,338,367 - bcp Sama_1910 0.57 -0.2 2,338,444 + bcp Sama_1910 0.73 +0.1 2,338,449 + bcp Sama_1910 0.74 +0.2 2,338,449 + bcp Sama_1910 0.74 +0.4 2,338,449 + bcp Sama_1910 0.74 -0.1 2,338,451 + bcp Sama_1910 0.75 +0.5 2,338,451 + bcp Sama_1910 0.75 +0.2 2,338,452 - bcp Sama_1910 0.75 -1.6 2,338,638 - -0.1 2,338,669 - +1.2 2,338,669 - -0.5 2,338,736 - +0.3 2,338,736 - +0.2 2,338,736 - -1.4 2,339,022 + Sama_1911 0.32 -1.8 2,339,023 - Sama_1911 0.32 -0.3 2,339,030 - Sama_1911 0.32 +0.5 2,339,030 - Sama_1911 0.32 -0.2
Or see this region's nucleotide sequence