Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dipZ and Sama_0539 overlap by 4 nucleotides Sama_0539 and Sama_0540 are separated by 212 nucleotides Sama_0540 and Sama_0541 are separated by 62 nucleotides
Sama_0538: dipZ - thiol:disulfide interchange protein precursor (RefSeq), at 663,245 to 665,068
dipZ
Sama_0539: Sama_0539 - periplasmic divalent cation tolerance protein CutA (RefSeq), at 665,065 to 665,397
_0539
Sama_0540: Sama_0540 - FxsA cytoplasmic membrane protein (RefSeq), at 665,610 to 666,200
_0540
Sama_0541: Sama_0541 - serine/threonine protein kinase, putative (RefSeq), at 666,263 to 668,083
_0541
Position (kb) 665
666
667 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 664.706 kb on - strand, within dipZ at 664.749 kb on + strand, within dipZ at 664.749 kb on + strand, within dipZ at 664.749 kb on + strand, within dipZ at 664.749 kb on + strand, within dipZ at 664.750 kb on - strand, within dipZ at 664.750 kb on - strand, within dipZ at 664.750 kb on - strand, within dipZ at 664.756 kb on - strand, within dipZ at 664.756 kb on - strand, within dipZ at 664.756 kb on - strand, within dipZ at 664.869 kb on + strand, within dipZ at 664.869 kb on + strand, within dipZ at 664.870 kb on - strand, within dipZ at 664.872 kb on + strand, within dipZ at 664.872 kb on + strand, within dipZ at 664.873 kb on - strand, within dipZ at 665.028 kb on - strand at 665.041 kb on + strand at 665.041 kb on + strand at 665.042 kb on - strand at 665.042 kb on - strand at 665.077 kb on - strand at 665.077 kb on - strand at 665.077 kb on - strand at 665.077 kb on - strand at 665.134 kb on - strand, within Sama_0539 at 665.151 kb on - strand, within Sama_0539 at 665.189 kb on + strand, within Sama_0539 at 665.189 kb on + strand, within Sama_0539 at 665.190 kb on - strand, within Sama_0539 at 665.190 kb on - strand, within Sama_0539 at 665.190 kb on - strand, within Sama_0539 at 665.190 kb on - strand, within Sama_0539 at 665.216 kb on - strand, within Sama_0539 at 665.216 kb on - strand, within Sama_0539 at 665.216 kb on - strand, within Sama_0539 at 665.220 kb on + strand, within Sama_0539 at 665.222 kb on + strand, within Sama_0539 at 665.223 kb on - strand, within Sama_0539 at 665.258 kb on + strand, within Sama_0539 at 665.259 kb on - strand, within Sama_0539 at 665.259 kb on - strand, within Sama_0539 at 665.259 kb on - strand, within Sama_0539 at 665.259 kb on - strand, within Sama_0539 at 665.374 kb on - strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.375 kb on + strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.376 kb on - strand at 665.415 kb on - strand at 665.426 kb on - strand at 665.426 kb on - strand at 665.426 kb on - strand at 665.426 kb on - strand at 665.434 kb on + strand at 665.434 kb on + strand at 665.434 kb on + strand at 665.434 kb on + strand at 665.434 kb on + strand at 665.434 kb on + strand at 665.434 kb on + strand at 665.434 kb on + strand at 665.435 kb on - strand at 665.435 kb on - strand at 665.435 kb on - strand at 665.435 kb on - strand at 665.439 kb on + strand at 665.439 kb on + strand at 665.439 kb on + strand at 665.440 kb on - strand at 665.445 kb on + strand at 665.468 kb on + strand at 665.468 kb on + strand at 665.468 kb on + strand at 665.469 kb on - strand at 665.469 kb on - strand at 665.469 kb on - strand at 665.493 kb on + strand at 665.493 kb on + strand at 665.494 kb on - strand at 665.494 kb on - strand at 665.509 kb on + strand at 665.548 kb on - strand at 665.579 kb on + strand at 665.579 kb on + strand at 665.596 kb on + strand at 665.597 kb on - strand at 665.731 kb on + strand, within Sama_0540 at 665.731 kb on + strand, within Sama_0540 at 665.731 kb on + strand, within Sama_0540 at 665.731 kb on + strand, within Sama_0540 at 665.732 kb on - strand, within Sama_0540 at 665.734 kb on + strand, within Sama_0540 at 665.734 kb on + strand, within Sama_0540 at 665.734 kb on + strand, within Sama_0540 at 665.735 kb on - strand, within Sama_0540 at 665.735 kb on - strand, within Sama_0540 at 665.813 kb on + strand, within Sama_0540 at 665.814 kb on - strand, within Sama_0540 at 665.828 kb on - strand, within Sama_0540 at 665.869 kb on + strand, within Sama_0540 at 666.164 kb on + strand at 666.164 kb on + strand at 666.164 kb on + strand at 666.164 kb on + strand at 666.164 kb on + strand at 666.164 kb on + strand at 666.165 kb on - strand at 666.165 kb on - strand at 666.278 kb on + strand at 666.278 kb on + strand at 666.279 kb on - strand at 666.279 kb on - strand at 666.301 kb on - strand at 666.365 kb on - strand at 666.395 kb on - strand at 666.454 kb on + strand, within Sama_0541 at 666.455 kb on - strand, within Sama_0541 at 666.455 kb on - strand, within Sama_0541 at 666.464 kb on + strand, within Sama_0541 at 666.464 kb on + strand, within Sama_0541 at 666.464 kb on + strand, within Sama_0541 at 666.464 kb on + strand, within Sama_0541 at 666.465 kb on - strand, within Sama_0541 at 666.465 kb on - strand, within Sama_0541 at 666.465 kb on - strand, within Sama_0541 at 666.468 kb on + strand, within Sama_0541 at 666.469 kb on - strand, within Sama_0541 at 666.511 kb on + strand, within Sama_0541 at 666.512 kb on - strand, within Sama_0541 at 666.512 kb on - strand, within Sama_0541 at 666.536 kb on + strand, within Sama_0541 at 666.571 kb on + strand, within Sama_0541 at 666.610 kb on - strand, within Sama_0541 at 666.644 kb on + strand, within Sama_0541 at 666.644 kb on + strand, within Sama_0541 at 666.644 kb on + strand, within Sama_0541 at 666.644 kb on + strand, within Sama_0541 at 666.644 kb on + strand, within Sama_0541 at 666.644 kb on + strand, within Sama_0541 at 666.645 kb on - strand, within Sama_0541 at 666.645 kb on - strand, within Sama_0541 at 666.665 kb on + strand, within Sama_0541 at 666.665 kb on + strand, within Sama_0541 at 666.666 kb on - strand, within Sama_0541 at 666.666 kb on - strand, within Sama_0541 at 666.666 kb on - strand, within Sama_0541 at 666.773 kb on + strand, within Sama_0541 at 666.773 kb on + strand, within Sama_0541 at 666.774 kb on - strand, within Sama_0541 at 666.774 kb on - strand, within Sama_0541 at 666.774 kb on - strand, within Sama_0541 at 666.862 kb on + strand, within Sama_0541 at 666.862 kb on + strand, within Sama_0541 at 666.862 kb on + strand, within Sama_0541 at 666.866 kb on + strand, within Sama_0541 at 666.866 kb on + strand, within Sama_0541 at 666.867 kb on - strand, within Sama_0541 at 666.867 kb on - strand, within Sama_0541 at 666.906 kb on - strand, within Sama_0541 at 666.913 kb on - strand, within Sama_0541 at 666.940 kb on + strand, within Sama_0541 at 666.941 kb on - strand, within Sama_0541 at 666.941 kb on - strand, within Sama_0541 at 666.984 kb on + strand, within Sama_0541 at 666.985 kb on - strand, within Sama_0541 at 666.987 kb on + strand, within Sama_0541 at 666.987 kb on + strand, within Sama_0541 at 666.987 kb on + strand, within Sama_0541 at 666.987 kb on + strand, within Sama_0541 at 666.987 kb on + strand, within Sama_0541 at 666.987 kb on + strand, within Sama_0541 at 666.987 kb on + strand, within Sama_0541 at 666.988 kb on - strand, within Sama_0541 at 666.988 kb on - strand, within Sama_0541 at 666.988 kb on - strand, within Sama_0541 at 666.988 kb on - strand, within Sama_0541 at 666.988 kb on - strand, within Sama_0541 at 666.988 kb on - strand, within Sama_0541 at 666.988 kb on - strand, within Sama_0541 at 666.991 kb on + strand, within Sama_0541 at 666.991 kb on + strand, within Sama_0541 at 666.992 kb on - strand, within Sama_0541 at 666.992 kb on - strand, within Sama_0541 at 666.992 kb on - strand, within Sama_0541 at 666.992 kb on - strand, within Sama_0541 at 666.992 kb on - strand, within Sama_0541 at 667.068 kb on + strand, within Sama_0541 at 667.068 kb on + strand, within Sama_0541 at 667.068 kb on + strand, within Sama_0541 at 667.068 kb on + strand, within Sama_0541 at 667.069 kb on - strand, within Sama_0541 at 667.069 kb on - strand, within Sama_0541 at 667.091 kb on - strand, within Sama_0541 at 667.177 kb on + strand, within Sama_0541 at 667.177 kb on + strand, within Sama_0541 at 667.177 kb on + strand, within Sama_0541 at 667.177 kb on + strand, within Sama_0541 at 667.178 kb on - strand, within Sama_0541 at 667.191 kb on + strand, within Sama_0541 at 667.192 kb on - strand, within Sama_0541 at 667.192 kb on - strand, within Sama_0541 at 667.192 kb on - strand, within Sama_0541 at 667.192 kb on - strand, within Sama_0541 at 667.192 kb on - strand, within Sama_0541
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 664,706 - dipZ Sama_0538 0.80 -2.5 664,749 + dipZ Sama_0538 0.82 -1.3 664,749 + dipZ Sama_0538 0.82 -1.9 664,749 + dipZ Sama_0538 0.82 -1.6 664,749 + dipZ Sama_0538 0.82 +0.7 664,750 - dipZ Sama_0538 0.83 -1.6 664,750 - dipZ Sama_0538 0.83 -2.3 664,750 - dipZ Sama_0538 0.83 -0.7 664,756 - dipZ Sama_0538 0.83 -1.2 664,756 - dipZ Sama_0538 0.83 -2.3 664,756 - dipZ Sama_0538 0.83 -1.3 664,869 + dipZ Sama_0538 0.89 -0.7 664,869 + dipZ Sama_0538 0.89 -0.9 664,870 - dipZ Sama_0538 0.89 -0.3 664,872 + dipZ Sama_0538 0.89 +0.7 664,872 + dipZ Sama_0538 0.89 -2.1 664,873 - dipZ Sama_0538 0.89 +1.7 665,028 - -0.0 665,041 + -1.6 665,041 + -1.5 665,042 - -0.9 665,042 - -1.1 665,077 - -1.2 665,077 - -0.5 665,077 - +0.5 665,077 - +0.4 665,134 - Sama_0539 0.21 -0.5 665,151 - Sama_0539 0.26 -0.5 665,189 + Sama_0539 0.37 -0.3 665,189 + Sama_0539 0.37 -2.9 665,190 - Sama_0539 0.38 -0.8 665,190 - Sama_0539 0.38 -0.3 665,190 - Sama_0539 0.38 -0.6 665,190 - Sama_0539 0.38 +0.0 665,216 - Sama_0539 0.45 +0.5 665,216 - Sama_0539 0.45 +0.2 665,216 - Sama_0539 0.45 -2.1 665,220 + Sama_0539 0.47 +0.9 665,222 + Sama_0539 0.47 -0.4 665,223 - Sama_0539 0.47 -0.1 665,258 + Sama_0539 0.58 +1.1 665,259 - Sama_0539 0.58 +0.6 665,259 - Sama_0539 0.58 -0.2 665,259 - Sama_0539 0.58 -0.8 665,259 - Sama_0539 0.58 +0.1 665,374 - -0.5 665,375 + -1.0 665,375 + -0.5 665,375 + -0.3 665,375 + -2.0 665,375 + +0.4 665,375 + +0.9 665,375 + +0.3 665,375 + -0.7 665,375 + -0.7 665,375 + +0.9 665,375 + -1.5 665,375 + +0.3 665,375 + +0.4 665,375 + +1.1 665,375 + -0.1 665,376 - +0.2 665,376 - -0.6 665,376 - -0.3 665,376 - -0.1 665,376 - -0.7 665,376 - -1.6 665,376 - -0.4 665,376 - -0.2 665,376 - +1.5 665,376 - -0.5 665,376 - -0.3 665,376 - +1.5 665,415 - -0.4 665,426 - +0.5 665,426 - +1.5 665,426 - -1.7 665,426 - +0.5 665,434 + -1.3 665,434 + +0.7 665,434 + -0.5 665,434 + +0.5 665,434 + +1.0 665,434 + -0.3 665,434 + -0.5 665,434 + -0.6 665,435 - +0.1 665,435 - -0.3 665,435 - -0.3 665,435 - -0.9 665,439 + -0.5 665,439 + -0.2 665,439 + +0.2 665,440 - -0.4 665,445 + -1.0 665,468 + +0.3 665,468 + +0.6 665,468 + +1.0 665,469 - +0.1 665,469 - +0.1 665,469 - -1.0 665,493 + -0.2 665,493 + +0.2 665,494 - +1.7 665,494 - +0.8 665,509 + +1.9 665,548 - +0.3 665,579 + +0.7 665,579 + +0.5 665,596 + +0.3 665,597 - -1.5 665,731 + Sama_0540 0.20 +1.7 665,731 + Sama_0540 0.20 -1.7 665,731 + Sama_0540 0.20 -3.1 665,731 + Sama_0540 0.20 -2.8 665,732 - Sama_0540 0.21 -1.7 665,734 + Sama_0540 0.21 -1.4 665,734 + Sama_0540 0.21 -2.3 665,734 + Sama_0540 0.21 -1.7 665,735 - Sama_0540 0.21 -1.7 665,735 - Sama_0540 0.21 -1.0 665,813 + Sama_0540 0.34 -0.6 665,814 - Sama_0540 0.35 -0.6 665,828 - Sama_0540 0.37 -1.9 665,869 + Sama_0540 0.44 -1.0 666,164 + -0.2 666,164 + +1.2 666,164 + -0.4 666,164 + +0.7 666,164 + +2.6 666,164 + -0.9 666,165 - -1.4 666,165 - -0.9 666,278 + +1.5 666,278 + +1.3 666,279 - -0.1 666,279 - -0.9 666,301 - -0.7 666,365 - -0.5 666,395 - -0.4 666,454 + Sama_0541 0.10 +0.5 666,455 - Sama_0541 0.11 -0.9 666,455 - Sama_0541 0.11 -0.1 666,464 + Sama_0541 0.11 -0.4 666,464 + Sama_0541 0.11 +0.1 666,464 + Sama_0541 0.11 -1.5 666,464 + Sama_0541 0.11 -1.4 666,465 - Sama_0541 0.11 +0.2 666,465 - Sama_0541 0.11 +0.7 666,465 - Sama_0541 0.11 -0.1 666,468 + Sama_0541 0.11 -0.9 666,469 - Sama_0541 0.11 -1.0 666,511 + Sama_0541 0.14 +0.2 666,512 - Sama_0541 0.14 +0.1 666,512 - Sama_0541 0.14 +0.0 666,536 + Sama_0541 0.15 +0.7 666,571 + Sama_0541 0.17 +0.1 666,610 - Sama_0541 0.19 -0.8 666,644 + Sama_0541 0.21 -0.8 666,644 + Sama_0541 0.21 +1.0 666,644 + Sama_0541 0.21 +0.5 666,644 + Sama_0541 0.21 -0.1 666,644 + Sama_0541 0.21 +1.1 666,644 + Sama_0541 0.21 +0.1 666,645 - Sama_0541 0.21 +0.9 666,645 - Sama_0541 0.21 +1.0 666,665 + Sama_0541 0.22 -0.9 666,665 + Sama_0541 0.22 -1.1 666,666 - Sama_0541 0.22 +1.3 666,666 - Sama_0541 0.22 +0.4 666,666 - Sama_0541 0.22 +0.3 666,773 + Sama_0541 0.28 +0.2 666,773 + Sama_0541 0.28 -1.4 666,774 - Sama_0541 0.28 +0.2 666,774 - Sama_0541 0.28 -0.9 666,774 - Sama_0541 0.28 -0.5 666,862 + Sama_0541 0.33 +2.3 666,862 + Sama_0541 0.33 +0.1 666,862 + Sama_0541 0.33 -0.1 666,866 + Sama_0541 0.33 -0.5 666,866 + Sama_0541 0.33 -0.1 666,867 - Sama_0541 0.33 -1.9 666,867 - Sama_0541 0.33 +0.5 666,906 - Sama_0541 0.35 +0.2 666,913 - Sama_0541 0.36 +0.9 666,940 + Sama_0541 0.37 -0.1 666,941 - Sama_0541 0.37 +0.5 666,941 - Sama_0541 0.37 +0.1 666,984 + Sama_0541 0.40 -2.1 666,985 - Sama_0541 0.40 +0.7 666,987 + Sama_0541 0.40 +0.0 666,987 + Sama_0541 0.40 +0.2 666,987 + Sama_0541 0.40 +1.1 666,987 + Sama_0541 0.40 +0.7 666,987 + Sama_0541 0.40 +1.8 666,987 + Sama_0541 0.40 -0.9 666,987 + Sama_0541 0.40 +0.5 666,988 - Sama_0541 0.40 -0.9 666,988 - Sama_0541 0.40 +0.0 666,988 - Sama_0541 0.40 -0.6 666,988 - Sama_0541 0.40 -0.4 666,988 - Sama_0541 0.40 +0.4 666,988 - Sama_0541 0.40 -1.0 666,988 - Sama_0541 0.40 -0.7 666,991 + Sama_0541 0.40 -0.4 666,991 + Sama_0541 0.40 +0.8 666,992 - Sama_0541 0.40 -0.6 666,992 - Sama_0541 0.40 -1.3 666,992 - Sama_0541 0.40 +0.7 666,992 - Sama_0541 0.40 +0.7 666,992 - Sama_0541 0.40 -1.1 667,068 + Sama_0541 0.44 -1.5 667,068 + Sama_0541 0.44 +0.1 667,068 + Sama_0541 0.44 -0.5 667,068 + Sama_0541 0.44 -0.1 667,069 - Sama_0541 0.44 -0.7 667,069 - Sama_0541 0.44 +0.7 667,091 - Sama_0541 0.45 -0.3 667,177 + Sama_0541 0.50 +0.4 667,177 + Sama_0541 0.50 +1.1 667,177 + Sama_0541 0.50 +0.7 667,177 + Sama_0541 0.50 -0.9 667,178 - Sama_0541 0.50 -0.0 667,191 + Sama_0541 0.51 +0.9 667,192 - Sama_0541 0.51 +1.1 667,192 - Sama_0541 0.51 +0.1 667,192 - Sama_0541 0.51 -0.6 667,192 - Sama_0541 0.51 +0.2 667,192 - Sama_0541 0.51 -0.9
Or see this region's nucleotide sequence