Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0517 and Sama_0518 are separated by 171 nucleotides Sama_0518 and Sama_0519 are separated by 9 nucleotides Sama_0519 and Sama_0520 are separated by 143 nucleotides
Sama_0517: Sama_0517 - D-beta-hydroxybutyrate dehydrogenase (RefSeq), at 636,932 to 637,732
_0517
Sama_0518: Sama_0518 - 3-oxoacid CoA-transferase (RefSeq), at 637,904 to 638,665
_0518
Sama_0519: Sama_0519 - 3-oxoacid CoA-transferase (RefSeq), at 638,675 to 639,340
_0519
Sama_0520: Sama_0520 - putative polyhydroxyalkanoate depolymerase (RefSeq), at 639,484 to 640,431
_0520
Position (kb) 638
639
640 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 637.690 kb on + strand at 637.691 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.733 kb on + strand at 637.733 kb on + strand at 637.734 kb on - strand at 637.734 kb on - strand at 637.734 kb on - strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.867 kb on + strand at 637.867 kb on + strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.883 kb on - strand at 637.883 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.913 kb on - strand at 637.913 kb on - strand at 637.913 kb on - strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.916 kb on - strand at 637.966 kb on + strand at 637.966 kb on + strand at 637.967 kb on - strand at 637.967 kb on - strand at 637.982 kb on + strand, within Sama_0518 at 637.982 kb on + strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 638.051 kb on - strand, within Sama_0518 at 638.135 kb on + strand, within Sama_0518 at 638.136 kb on - strand, within Sama_0518 at 638.136 kb on - strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.203 kb on + strand, within Sama_0518 at 638.299 kb on + strand, within Sama_0518 at 638.301 kb on + strand, within Sama_0518 at 638.313 kb on - strand, within Sama_0518 at 638.382 kb on - strand, within Sama_0518 at 638.382 kb on - strand, within Sama_0518 at 638.441 kb on - strand, within Sama_0518 at 638.444 kb on + strand, within Sama_0518 at 638.445 kb on - strand, within Sama_0518 at 638.457 kb on + strand, within Sama_0518 at 638.458 kb on - strand, within Sama_0518 at 638.511 kb on + strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.585 kb on + strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.659 kb on + strand at 638.659 kb on + strand at 638.659 kb on + strand at 638.660 kb on - strand at 638.663 kb on + strand at 638.663 kb on + strand at 638.664 kb on - strand at 638.664 kb on - strand at 638.664 kb on - strand at 638.732 kb on + strand at 638.733 kb on - strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.736 kb on - strand at 638.736 kb on - strand at 638.751 kb on + strand, within Sama_0519 at 638.752 kb on - strand, within Sama_0519 at 638.752 kb on - strand, within Sama_0519 at 638.773 kb on + strand, within Sama_0519 at 638.773 kb on + strand, within Sama_0519 at 638.774 kb on - strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.793 kb on + strand, within Sama_0519 at 638.793 kb on + strand, within Sama_0519 at 638.793 kb on + strand, within Sama_0519 at 638.793 kb on + strand, within Sama_0519 at 638.794 kb on - strand, within Sama_0519 at 638.794 kb on - strand, within Sama_0519 at 638.873 kb on - strand, within Sama_0519 at 638.970 kb on + strand, within Sama_0519 at 638.970 kb on + strand, within Sama_0519 at 638.971 kb on - strand, within Sama_0519 at 638.971 kb on - strand, within Sama_0519 at 638.971 kb on - strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.021 kb on - strand, within Sama_0519 at 639.021 kb on - strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.176 kb on - strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.272 kb on - strand, within Sama_0519 at 639.272 kb on - strand, within Sama_0519 at 639.339 kb on - strand at 639.339 kb on - strand at 639.339 kb on - strand at 639.410 kb on + strand at 639.410 kb on + strand at 639.410 kb on + strand at 639.410 kb on + strand at 639.411 kb on - strand at 639.411 kb on - strand at 639.411 kb on - strand at 639.411 kb on - strand at 639.440 kb on - strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.461 kb on - strand at 639.461 kb on - strand at 639.461 kb on - strand at 639.461 kb on - strand at 639.483 kb on + strand at 639.484 kb on - strand at 639.484 kb on - strand at 639.527 kb on + strand at 639.528 kb on - strand at 639.528 kb on - strand at 639.528 kb on - strand at 639.534 kb on + strand at 639.534 kb on + strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.631 kb on + strand, within Sama_0520 at 639.631 kb on + strand, within Sama_0520 at 639.631 kb on + strand, within Sama_0520 at 639.631 kb on + strand, within Sama_0520 at 639.632 kb on - strand, within Sama_0520 at 639.788 kb on + strand, within Sama_0520 at 639.789 kb on - strand, within Sama_0520 at 639.823 kb on - strand, within Sama_0520 at 639.855 kb on + strand, within Sama_0520 at 639.867 kb on + strand, within Sama_0520 at 639.867 kb on + strand, within Sama_0520 at 639.868 kb on - strand, within Sama_0520 at 639.904 kb on + strand, within Sama_0520 at 639.904 kb on + strand, within Sama_0520 at 639.904 kb on + strand, within Sama_0520 at 639.904 kb on + strand, within Sama_0520 at 639.905 kb on - strand, within Sama_0520 at 639.917 kb on + strand, within Sama_0520 at 639.917 kb on + strand, within Sama_0520 at 639.918 kb on - strand, within Sama_0520 at 639.918 kb on - strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.955 kb on - strand, within Sama_0520 at 639.955 kb on - strand, within Sama_0520 at 639.955 kb on - strand, within Sama_0520 at 639.955 kb on - strand, within Sama_0520 at 639.989 kb on + strand, within Sama_0520 at 640.003 kb on + strand, within Sama_0520 at 640.004 kb on - strand, within Sama_0520 at 640.097 kb on + strand, within Sama_0520 at 640.214 kb on + strand, within Sama_0520 at 640.215 kb on - strand, within Sama_0520 at 640.215 kb on - strand, within Sama_0520 at 640.215 kb on - strand, within Sama_0520 at 640.215 kb on - strand, within Sama_0520 at 640.308 kb on + strand, within Sama_0520 at 640.309 kb on - strand, within Sama_0520 at 640.309 kb on - strand, within Sama_0520 at 640.311 kb on + strand, within Sama_0520 at 640.311 kb on + strand, within Sama_0520
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 637,690 + -0.1 637,691 - -0.9 637,710 - -0.4 637,710 - +0.2 637,710 - -0.4 637,710 - +1.3 637,733 + -0.8 637,733 + -0.6 637,734 - -1.5 637,734 - -0.3 637,734 - -0.5 637,762 + -1.2 637,762 + -0.5 637,762 + -0.1 637,762 + -0.0 637,762 + +0.7 637,762 + +0.5 637,762 + +0.1 637,762 + +0.1 637,762 + +0.3 637,762 + +0.6 637,763 - +1.7 637,763 - +1.0 637,763 - +0.1 637,763 - +0.6 637,763 - -1.9 637,763 - +0.1 637,763 - +0.6 637,763 - -0.9 637,763 - +0.3 637,763 - -0.1 637,763 - -0.1 637,763 - +1.1 637,867 + +0.7 637,867 + +0.4 637,868 - +0.0 637,868 - +0.2 637,868 - +0.5 637,868 - +0.6 637,868 - +0.6 637,879 + -1.9 637,879 + +0.3 637,879 + -0.3 637,879 + -1.0 637,879 + -0.3 637,879 + +1.0 637,879 + -0.6 637,879 + -0.9 637,879 + +0.8 637,879 + +0.7 637,880 - -0.9 637,880 - +0.0 637,880 - +0.9 637,880 - -0.3 637,880 - +0.1 637,880 - -0.3 637,880 - -0.3 637,880 - +0.5 637,880 - +0.7 637,880 - +0.6 637,882 + -0.2 637,882 + -2.3 637,882 + -0.5 637,882 + -0.9 637,883 - +1.0 637,883 - -0.5 637,888 - -0.3 637,888 - -0.7 637,888 - +0.3 637,888 - -0.8 637,888 - +0.1 637,888 - -0.4 637,912 + +0.6 637,912 + -0.3 637,912 + +0.6 637,912 + +2.0 637,912 + +0.0 637,913 - -1.6 637,913 - -0.4 637,913 - +0.4 637,915 + +0.7 637,915 + -0.8 637,915 + -0.6 637,915 + +0.1 637,916 - -0.5 637,966 + -0.4 637,966 + -0.6 637,967 - -0.8 637,967 - -0.1 637,982 + Sama_0518 0.10 +0.1 637,982 + Sama_0518 0.10 +0.5 637,983 - Sama_0518 0.10 +0.8 637,983 - Sama_0518 0.10 +1.0 637,983 - Sama_0518 0.10 +0.1 637,983 - Sama_0518 0.10 +1.0 638,051 - Sama_0518 0.19 -0.9 638,135 + Sama_0518 0.30 -0.9 638,136 - Sama_0518 0.30 +0.4 638,136 - Sama_0518 0.30 +0.4 638,189 + Sama_0518 0.37 -0.3 638,189 + Sama_0518 0.37 -0.2 638,189 + Sama_0518 0.37 +0.8 638,189 + Sama_0518 0.37 +0.4 638,189 + Sama_0518 0.37 +2.0 638,190 - Sama_0518 0.38 +0.5 638,190 - Sama_0518 0.38 +0.5 638,190 - Sama_0518 0.38 -1.5 638,190 - Sama_0518 0.38 +0.3 638,190 - Sama_0518 0.38 +0.0 638,190 - Sama_0518 0.38 +0.2 638,203 + Sama_0518 0.39 -1.3 638,299 + Sama_0518 0.52 -0.9 638,301 + Sama_0518 0.52 -0.6 638,313 - Sama_0518 0.54 -0.8 638,382 - Sama_0518 0.63 -1.0 638,382 - Sama_0518 0.63 +0.0 638,441 - Sama_0518 0.70 +0.9 638,444 + Sama_0518 0.71 -0.4 638,445 - Sama_0518 0.71 +0.7 638,457 + Sama_0518 0.73 +0.6 638,458 - Sama_0518 0.73 -0.3 638,511 + Sama_0518 0.80 -0.9 638,512 - Sama_0518 0.80 -0.1 638,512 - Sama_0518 0.80 -0.6 638,512 - Sama_0518 0.80 +0.3 638,512 - Sama_0518 0.80 -0.2 638,512 - Sama_0518 0.80 -0.5 638,585 + Sama_0518 0.89 -0.6 638,586 - Sama_0518 0.90 +0.6 638,586 - Sama_0518 0.90 -0.1 638,586 - Sama_0518 0.90 +0.0 638,659 + +0.3 638,659 + -2.1 638,659 + -0.1 638,660 - +2.5 638,663 + -0.2 638,663 + -0.7 638,664 - -2.0 638,664 - +0.1 638,664 - -2.7 638,732 + -0.8 638,733 - +0.6 638,735 + -0.7 638,735 + -0.3 638,735 + +0.7 638,735 + -0.0 638,735 + -1.4 638,735 + +0.0 638,735 + -1.6 638,736 - -0.0 638,736 - -1.3 638,751 + Sama_0519 0.11 -0.4 638,752 - Sama_0519 0.12 -0.4 638,752 - Sama_0519 0.12 -1.9 638,773 + Sama_0519 0.15 -0.2 638,773 + Sama_0519 0.15 +0.7 638,774 - Sama_0519 0.15 +0.8 638,777 + Sama_0519 0.15 +1.0 638,777 + Sama_0519 0.15 -0.3 638,777 + Sama_0519 0.15 -0.0 638,777 + Sama_0519 0.15 -1.9 638,777 + Sama_0519 0.15 -0.1 638,777 + Sama_0519 0.15 +0.3 638,778 - Sama_0519 0.15 -1.5 638,778 - Sama_0519 0.15 -0.7 638,778 - Sama_0519 0.15 +0.5 638,778 - Sama_0519 0.15 +0.5 638,778 - Sama_0519 0.15 +0.1 638,778 - Sama_0519 0.15 -0.1 638,778 - Sama_0519 0.15 +1.7 638,793 + Sama_0519 0.18 +0.3 638,793 + Sama_0519 0.18 -0.2 638,793 + Sama_0519 0.18 +0.4 638,793 + Sama_0519 0.18 -0.6 638,794 - Sama_0519 0.18 +0.2 638,794 - Sama_0519 0.18 +0.1 638,873 - Sama_0519 0.30 +0.1 638,970 + Sama_0519 0.44 +0.2 638,970 + Sama_0519 0.44 -0.0 638,971 - Sama_0519 0.44 +0.6 638,971 - Sama_0519 0.44 +0.9 638,971 - Sama_0519 0.44 +0.0 639,020 + Sama_0519 0.52 +0.6 639,020 + Sama_0519 0.52 -0.7 639,020 + Sama_0519 0.52 -1.7 639,020 + Sama_0519 0.52 -0.1 639,020 + Sama_0519 0.52 +1.3 639,020 + Sama_0519 0.52 -2.2 639,020 + Sama_0519 0.52 +1.3 639,021 - Sama_0519 0.52 -2.0 639,021 - Sama_0519 0.52 +0.2 639,093 + Sama_0519 0.63 +0.2 639,093 + Sama_0519 0.63 +0.9 639,093 + Sama_0519 0.63 -0.1 639,093 + Sama_0519 0.63 -0.7 639,093 + Sama_0519 0.63 +1.1 639,093 + Sama_0519 0.63 +0.2 639,094 - Sama_0519 0.63 -0.4 639,094 - Sama_0519 0.63 -0.3 639,094 - Sama_0519 0.63 -0.9 639,094 - Sama_0519 0.63 -0.0 639,094 - Sama_0519 0.63 -0.3 639,094 - Sama_0519 0.63 -0.3 639,094 - Sama_0519 0.63 -2.1 639,094 - Sama_0519 0.63 -1.0 639,094 - Sama_0519 0.63 -1.9 639,094 - Sama_0519 0.63 +0.7 639,094 - Sama_0519 0.63 -1.6 639,094 - Sama_0519 0.63 -0.6 639,176 - Sama_0519 0.75 +0.2 639,271 + Sama_0519 0.89 +0.9 639,271 + Sama_0519 0.89 +0.5 639,271 + Sama_0519 0.89 -0.7 639,271 + Sama_0519 0.89 +2.0 639,271 + Sama_0519 0.89 -0.6 639,272 - Sama_0519 0.90 -0.3 639,272 - Sama_0519 0.90 -0.0 639,339 - +0.2 639,339 - +0.3 639,339 - -0.1 639,410 + -1.2 639,410 + -0.1 639,410 + -1.4 639,410 + -0.3 639,411 - +0.3 639,411 - +0.9 639,411 - +0.3 639,411 - +0.5 639,440 - -0.3 639,460 + -0.3 639,460 + -0.4 639,460 + -0.5 639,460 + -0.5 639,460 + -0.6 639,460 + +0.7 639,461 - -0.4 639,461 - -0.9 639,461 - +0.2 639,461 - -0.0 639,483 + +0.0 639,484 - -1.6 639,484 - -0.1 639,527 + -0.5 639,528 - +1.0 639,528 - +0.5 639,528 - +0.1 639,534 + +0.2 639,534 + -0.7 639,535 - -0.6 639,535 - -0.2 639,535 - -0.6 639,535 - +1.0 639,535 - -0.3 639,535 - -2.9 639,535 - -1.0 639,631 + Sama_0520 0.16 +0.0 639,631 + Sama_0520 0.16 -0.7 639,631 + Sama_0520 0.16 +0.6 639,631 + Sama_0520 0.16 +0.1 639,632 - Sama_0520 0.16 +0.0 639,788 + Sama_0520 0.32 -0.5 639,789 - Sama_0520 0.32 +0.4 639,823 - Sama_0520 0.36 +0.4 639,855 + Sama_0520 0.39 -0.6 639,867 + Sama_0520 0.40 +0.1 639,867 + Sama_0520 0.40 -0.4 639,868 - Sama_0520 0.41 -0.4 639,904 + Sama_0520 0.44 -1.9 639,904 + Sama_0520 0.44 -0.8 639,904 + Sama_0520 0.44 -0.4 639,904 + Sama_0520 0.44 +0.2 639,905 - Sama_0520 0.44 -1.1 639,917 + Sama_0520 0.46 -0.3 639,917 + Sama_0520 0.46 -1.2 639,918 - Sama_0520 0.46 +0.3 639,918 - Sama_0520 0.46 -1.3 639,954 + Sama_0520 0.50 +0.3 639,954 + Sama_0520 0.50 +0.3 639,954 + Sama_0520 0.50 +0.7 639,954 + Sama_0520 0.50 +0.7 639,954 + Sama_0520 0.50 +0.2 639,955 - Sama_0520 0.50 -0.1 639,955 - Sama_0520 0.50 +0.4 639,955 - Sama_0520 0.50 -0.6 639,955 - Sama_0520 0.50 -0.9 639,989 + Sama_0520 0.53 +0.3 640,003 + Sama_0520 0.55 +0.9 640,004 - Sama_0520 0.55 -0.3 640,097 + Sama_0520 0.65 +1.9 640,214 + Sama_0520 0.77 +0.1 640,215 - Sama_0520 0.77 -0.7 640,215 - Sama_0520 0.77 +0.3 640,215 - Sama_0520 0.77 -0.5 640,215 - Sama_0520 0.77 +1.1 640,308 + Sama_0520 0.87 +0.2 640,309 - Sama_0520 0.87 -0.0 640,309 - Sama_0520 0.87 +1.6 640,311 + Sama_0520 0.87 -0.1 640,311 + Sama_0520 0.87 +0.1
Or see this region's nucleotide sequence