Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hslU and Sama_0441 are separated by 9 nucleotides Sama_0441 and Sama_0442 are separated by 205 nucleotides Sama_0442 and argS are separated by 3 nucleotides
Sama_0440: hslU - ATP-dependent protease ATP-binding subunit HslU (RefSeq), at 542,817 to 544,142
hslU
Sama_0441: Sama_0441 - ATP-dependent protease peptidase subunit (RefSeq), at 544,152 to 544,676
_0441
Sama_0442: Sama_0442 - sporulation related (RefSeq), at 544,882 to 545,439
_0442
Sama_0443: argS - arginyl-tRNA synthetase (RefSeq), at 545,443 to 547,188
argS
Position (kb) 544
545 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 543.246 kb on - strand, within hslU at 543.802 kb on - strand, within hslU at 543.871 kb on + strand, within hslU at 543.872 kb on - strand, within hslU at 543.956 kb on + strand, within hslU at 543.987 kb on - strand, within hslU at 544.094 kb on + strand at 544.157 kb on - strand at 544.157 kb on - strand at 544.203 kb on - strand at 544.338 kb on + strand, within Sama_0441 at 544.339 kb on - strand, within Sama_0441 at 544.339 kb on - strand, within Sama_0441 at 544.339 kb on - strand, within Sama_0441 at 544.549 kb on - strand, within Sama_0441 at 544.549 kb on - strand, within Sama_0441 at 544.562 kb on - strand, within Sama_0441 at 544.667 kb on + strand at 544.667 kb on + strand at 544.668 kb on - strand at 544.668 kb on - strand at 544.670 kb on + strand at 544.671 kb on - strand at 544.692 kb on - strand at 544.692 kb on - strand at 544.706 kb on - strand at 544.730 kb on + strand at 544.730 kb on + strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.732 kb on + strand at 544.732 kb on + strand at 544.733 kb on - strand at 544.733 kb on - strand at 544.733 kb on - strand at 544.733 kb on - strand at 544.762 kb on + strand at 544.763 kb on - strand at 544.792 kb on + strand at 544.792 kb on + strand at 544.793 kb on - strand at 544.793 kb on - strand at 544.793 kb on - strand at 544.793 kb on - strand at 544.869 kb on + strand at 544.869 kb on + strand at 544.869 kb on + strand at 544.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 543,246 - hslU Sama_0440 0.32 -3.1 543,802 - hslU Sama_0440 0.74 -2.3 543,871 + hslU Sama_0440 0.79 -2.6 543,872 - hslU Sama_0440 0.80 -0.3 543,956 + hslU Sama_0440 0.86 -1.1 543,987 - hslU Sama_0440 0.88 -2.8 544,094 + -2.0 544,157 - -1.4 544,157 - -1.5 544,203 - -1.9 544,338 + Sama_0441 0.35 -2.6 544,339 - Sama_0441 0.36 -3.7 544,339 - Sama_0441 0.36 -2.3 544,339 - Sama_0441 0.36 +0.3 544,549 - Sama_0441 0.76 -2.3 544,549 - Sama_0441 0.76 -2.0 544,562 - Sama_0441 0.78 -1.7 544,667 + -2.2 544,667 + -1.8 544,668 - -1.8 544,668 - -2.1 544,670 + -2.1 544,671 - -1.8 544,692 - -0.6 544,692 - -0.8 544,706 - -0.4 544,730 + -2.2 544,730 + -2.6 544,731 - -1.5 544,731 - -0.6 544,731 - -0.3 544,731 - -0.5 544,731 - -1.5 544,731 - -0.5 544,731 - -1.6 544,731 - +0.3 544,731 - +0.1 544,731 - -1.5 544,731 - -1.0 544,731 - -0.8 544,731 - -1.0 544,731 - -0.3 544,731 - +0.7 544,731 - -0.4 544,731 - +0.3 544,731 - -0.6 544,731 - -1.6 544,731 - +0.2 544,731 - +0.4 544,731 - -0.4 544,732 + -1.4 544,732 + -1.4 544,733 - -1.1 544,733 - -0.4 544,733 - +2.3 544,733 - -1.3 544,762 + +0.6 544,763 - -0.1 544,792 + -0.2 544,792 + -0.2 544,793 - -0.1 544,793 - -0.5 544,793 - +0.2 544,793 - +1.3 544,869 + +1.5 544,869 + -0.3 544,869 + -0.8 544,870 - -0.7
Or see this region's nucleotide sequence