Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0439 and hslU are separated by 162 nucleotides hslU and Sama_0441 are separated by 9 nucleotides Sama_0441 and Sama_0442 are separated by 205 nucleotides
Sama_0439: Sama_0439 - subtilisin-like serine protease-like protein (RefSeq), at 539,199 to 542,654
_0439
Sama_0440: hslU - ATP-dependent protease ATP-binding subunit HslU (RefSeq), at 542,817 to 544,142
hslU
Sama_0441: Sama_0441 - ATP-dependent protease peptidase subunit (RefSeq), at 544,152 to 544,676
_0441
Sama_0442: Sama_0442 - sporulation related (RefSeq), at 544,882 to 545,439
_0442
Position (kb) 542
543
544
545 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 541.850 kb on + strand, within Sama_0439 at 541.850 kb on + strand, within Sama_0439 at 541.850 kb on + strand, within Sama_0439 at 541.851 kb on - strand, within Sama_0439 at 541.851 kb on - strand, within Sama_0439 at 541.851 kb on - strand, within Sama_0439 at 541.851 kb on - strand, within Sama_0439 at 541.851 kb on - strand, within Sama_0439 at 541.851 kb on - strand, within Sama_0439 at 541.853 kb on + strand, within Sama_0439 at 541.854 kb on - strand, within Sama_0439 at 541.913 kb on + strand, within Sama_0439 at 541.914 kb on - strand, within Sama_0439 at 541.930 kb on + strand, within Sama_0439 at 541.930 kb on + strand, within Sama_0439 at 541.930 kb on + strand, within Sama_0439 at 541.931 kb on - strand, within Sama_0439 at 542.018 kb on + strand, within Sama_0439 at 542.028 kb on + strand, within Sama_0439 at 542.028 kb on + strand, within Sama_0439 at 542.029 kb on - strand, within Sama_0439 at 542.029 kb on - strand, within Sama_0439 at 542.029 kb on - strand, within Sama_0439 at 542.117 kb on + strand, within Sama_0439 at 542.128 kb on + strand, within Sama_0439 at 542.128 kb on + strand, within Sama_0439 at 542.298 kb on + strand, within Sama_0439 at 542.298 kb on + strand, within Sama_0439 at 542.298 kb on + strand, within Sama_0439 at 542.298 kb on + strand, within Sama_0439 at 542.298 kb on + strand, within Sama_0439 at 542.298 kb on + strand, within Sama_0439 at 542.298 kb on + strand, within Sama_0439 at 542.299 kb on - strand, within Sama_0439 at 542.299 kb on - strand, within Sama_0439 at 542.299 kb on - strand, within Sama_0439 at 542.316 kb on + strand at 542.316 kb on + strand at 542.317 kb on - strand at 542.427 kb on - strand at 542.434 kb on + strand at 542.434 kb on + strand at 542.434 kb on + strand at 542.435 kb on - strand at 542.435 kb on - strand at 542.435 kb on - strand at 542.435 kb on - strand at 542.435 kb on - strand at 542.484 kb on + strand at 542.485 kb on - strand at 542.568 kb on + strand at 542.648 kb on + strand at 542.648 kb on + strand at 542.648 kb on + strand at 542.648 kb on + strand at 542.648 kb on + strand at 542.649 kb on - strand at 542.649 kb on - strand at 542.652 kb on + strand at 542.652 kb on + strand at 542.652 kb on + strand at 542.652 kb on + strand at 542.652 kb on + strand at 542.652 kb on + strand at 542.653 kb on - strand at 542.653 kb on - strand at 542.653 kb on - strand at 542.653 kb on - strand at 542.653 kb on - strand at 542.653 kb on - strand at 542.721 kb on - strand at 542.721 kb on - strand at 542.768 kb on + strand at 542.769 kb on - strand at 542.769 kb on - strand at 542.774 kb on + strand at 542.774 kb on + strand at 542.775 kb on - strand at 542.785 kb on + strand at 542.785 kb on + strand at 542.785 kb on + strand at 542.786 kb on - strand at 542.786 kb on - strand at 542.786 kb on - strand at 542.786 kb on - strand at 542.786 kb on - strand at 542.788 kb on + strand at 542.788 kb on + strand at 542.795 kb on + strand at 542.795 kb on + strand at 542.818 kb on + strand at 542.818 kb on + strand at 542.818 kb on + strand at 542.818 kb on + strand at 542.819 kb on - strand at 542.826 kb on - strand at 542.858 kb on + strand at 542.889 kb on + strand at 542.889 kb on + strand at 542.889 kb on + strand at 542.890 kb on - strand at 542.890 kb on - strand at 542.890 kb on - strand at 542.890 kb on - strand at 542.890 kb on - strand at 543.085 kb on + strand, within hslU at 543.086 kb on - strand, within hslU at 543.123 kb on - strand, within hslU at 543.246 kb on - strand, within hslU at 543.802 kb on - strand, within hslU at 543.871 kb on + strand, within hslU at 543.872 kb on - strand, within hslU at 543.956 kb on + strand, within hslU at 543.987 kb on - strand, within hslU at 544.094 kb on + strand at 544.157 kb on - strand at 544.157 kb on - strand at 544.203 kb on - strand at 544.338 kb on + strand, within Sama_0441 at 544.339 kb on - strand, within Sama_0441 at 544.339 kb on - strand, within Sama_0441 at 544.339 kb on - strand, within Sama_0441 at 544.549 kb on - strand, within Sama_0441 at 544.549 kb on - strand, within Sama_0441 at 544.562 kb on - strand, within Sama_0441 at 544.667 kb on + strand at 544.667 kb on + strand at 544.668 kb on - strand at 544.668 kb on - strand at 544.670 kb on + strand at 544.671 kb on - strand at 544.692 kb on - strand at 544.692 kb on - strand at 544.706 kb on - strand at 544.730 kb on + strand at 544.730 kb on + strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.731 kb on - strand at 544.732 kb on + strand at 544.732 kb on + strand at 544.733 kb on - strand at 544.733 kb on - strand at 544.733 kb on - strand at 544.733 kb on - strand at 544.762 kb on + strand at 544.763 kb on - strand at 544.792 kb on + strand at 544.792 kb on + strand at 544.793 kb on - strand at 544.793 kb on - strand at 544.793 kb on - strand at 544.793 kb on - strand at 544.869 kb on + strand at 544.869 kb on + strand at 544.869 kb on + strand at 544.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 541,850 + Sama_0439 0.77 -0.2 541,850 + Sama_0439 0.77 +0.2 541,850 + Sama_0439 0.77 +0.0 541,851 - Sama_0439 0.77 -0.4 541,851 - Sama_0439 0.77 -0.4 541,851 - Sama_0439 0.77 -0.6 541,851 - Sama_0439 0.77 +0.5 541,851 - Sama_0439 0.77 +0.1 541,851 - Sama_0439 0.77 +0.3 541,853 + Sama_0439 0.77 +0.4 541,854 - Sama_0439 0.77 -0.4 541,913 + Sama_0439 0.79 -1.3 541,914 - Sama_0439 0.79 +0.9 541,930 + Sama_0439 0.79 -0.0 541,930 + Sama_0439 0.79 +0.2 541,930 + Sama_0439 0.79 +0.1 541,931 - Sama_0439 0.79 +0.4 542,018 + Sama_0439 0.82 -2.7 542,028 + Sama_0439 0.82 -0.1 542,028 + Sama_0439 0.82 +1.4 542,029 - Sama_0439 0.82 -0.3 542,029 - Sama_0439 0.82 +0.4 542,029 - Sama_0439 0.82 -1.6 542,117 + Sama_0439 0.84 -0.1 542,128 + Sama_0439 0.85 -0.3 542,128 + Sama_0439 0.85 +1.7 542,298 + Sama_0439 0.90 -1.8 542,298 + Sama_0439 0.90 -0.6 542,298 + Sama_0439 0.90 -0.8 542,298 + Sama_0439 0.90 +1.1 542,298 + Sama_0439 0.90 +0.1 542,298 + Sama_0439 0.90 -0.4 542,298 + Sama_0439 0.90 +0.3 542,299 - Sama_0439 0.90 -0.6 542,299 - Sama_0439 0.90 +0.2 542,299 - Sama_0439 0.90 -0.4 542,316 + +0.7 542,316 + +0.0 542,317 - +0.5 542,427 - +0.0 542,434 + +1.6 542,434 + -1.1 542,434 + -2.8 542,435 - -1.8 542,435 - -0.5 542,435 - +0.4 542,435 - -0.3 542,435 - -0.3 542,484 + -0.9 542,485 - +0.1 542,568 + -0.4 542,648 + -1.8 542,648 + -1.3 542,648 + -0.4 542,648 + -1.4 542,648 + -0.4 542,649 - -0.0 542,649 - -0.7 542,652 + +0.7 542,652 + -0.1 542,652 + +0.6 542,652 + +0.6 542,652 + +0.7 542,652 + +0.1 542,653 - +0.3 542,653 - +0.7 542,653 - -0.3 542,653 - +0.4 542,653 - +0.8 542,653 - -0.0 542,721 - -2.4 542,721 - -0.5 542,768 + -0.5 542,769 - +0.8 542,769 - -1.5 542,774 + +0.3 542,774 + -1.0 542,775 - +0.6 542,785 + +0.3 542,785 + -0.3 542,785 + +0.3 542,786 - -0.5 542,786 - -1.0 542,786 - +0.4 542,786 - +0.0 542,786 - +1.7 542,788 + -2.2 542,788 + -0.3 542,795 + -1.1 542,795 + -0.1 542,818 + -0.4 542,818 + +0.9 542,818 + -1.0 542,818 + -1.3 542,819 - -1.8 542,826 - -3.1 542,858 + -1.3 542,889 + -1.6 542,889 + -1.0 542,889 + -2.4 542,890 - -1.3 542,890 - +0.2 542,890 - +0.2 542,890 - -0.8 542,890 - -1.3 543,085 + hslU Sama_0440 0.20 -4.1 543,086 - hslU Sama_0440 0.20 +0.3 543,123 - hslU Sama_0440 0.23 -0.5 543,246 - hslU Sama_0440 0.32 -3.1 543,802 - hslU Sama_0440 0.74 -2.3 543,871 + hslU Sama_0440 0.79 -2.6 543,872 - hslU Sama_0440 0.80 -0.3 543,956 + hslU Sama_0440 0.86 -1.1 543,987 - hslU Sama_0440 0.88 -2.8 544,094 + -2.0 544,157 - -1.4 544,157 - -1.5 544,203 - -1.9 544,338 + Sama_0441 0.35 -2.6 544,339 - Sama_0441 0.36 -3.7 544,339 - Sama_0441 0.36 -2.3 544,339 - Sama_0441 0.36 +0.3 544,549 - Sama_0441 0.76 -2.3 544,549 - Sama_0441 0.76 -2.0 544,562 - Sama_0441 0.78 -1.7 544,667 + -2.2 544,667 + -1.8 544,668 - -1.8 544,668 - -2.1 544,670 + -2.1 544,671 - -1.8 544,692 - -0.6 544,692 - -0.8 544,706 - -0.4 544,730 + -2.2 544,730 + -2.6 544,731 - -1.5 544,731 - -0.6 544,731 - -0.3 544,731 - -0.5 544,731 - -1.5 544,731 - -0.5 544,731 - -1.6 544,731 - +0.3 544,731 - +0.1 544,731 - -1.5 544,731 - -1.0 544,731 - -0.8 544,731 - -1.0 544,731 - -0.3 544,731 - +0.7 544,731 - -0.4 544,731 - +0.3 544,731 - -0.6 544,731 - -1.6 544,731 - +0.2 544,731 - +0.4 544,731 - -0.4 544,732 + -1.4 544,732 + -1.4 544,733 - -1.1 544,733 - -0.4 544,733 - +2.3 544,733 - -1.3 544,762 + +0.6 544,763 - -0.1 544,792 + -0.2 544,792 + -0.2 544,793 - -0.1 544,793 - -0.5 544,793 - +0.2 544,793 - +1.3 544,869 + +1.5 544,869 + -0.3 544,869 + -0.8 544,870 - -0.7
Or see this region's nucleotide sequence