Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0319 and Sama_0320 are separated by 141 nucleotides Sama_0320 and pyrE are separated by 106 nucleotides pyrE and folE are separated by 101 nucleotides folE and Sama_0323 are separated by 182 nucleotides
Sama_0319: Sama_0319 - hypothetical protein (RefSeq), at 386,521 to 387,450
_0319
Sama_0320: Sama_0320 - tRNA nucleotidyltransferase (RefSeq), at 387,592 to 388,305
_0320
Sama_0321: pyrE - orotate phosphoribosyltransferase (RefSeq), at 388,412 to 389,053
pyrE
Sama_0322: folE - GTP cyclohydrolase I (RefSeq), at 389,155 to 389,805
folE
Sama_0323: Sama_0323 - prolyl oligopeptidase family protein (RefSeq), at 389,988 to 391,937
_0323
Position (kb) 388
389
390 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 387.424 kb on + strand at 387.424 kb on + strand at 387.424 kb on + strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.426 kb on + strand at 387.426 kb on + strand at 387.426 kb on + strand at 387.427 kb on - strand at 387.476 kb on + strand at 387.489 kb on + strand at 387.490 kb on - strand at 387.536 kb on + strand at 387.537 kb on - strand at 387.537 kb on - strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.544 kb on + strand at 387.545 kb on - strand at 387.545 kb on - strand at 387.545 kb on - strand at 387.545 kb on - strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.548 kb on - strand at 387.574 kb on + strand at 387.574 kb on + strand at 387.574 kb on + strand at 387.575 kb on - strand at 387.645 kb on + strand at 387.652 kb on - strand at 387.731 kb on + strand, within Sama_0320 at 387.731 kb on + strand, within Sama_0320 at 387.744 kb on + strand, within Sama_0320 at 387.779 kb on - strand, within Sama_0320 at 387.798 kb on + strand, within Sama_0320 at 387.799 kb on - strand, within Sama_0320 at 387.811 kb on - strand, within Sama_0320 at 387.902 kb on + strand, within Sama_0320 at 387.903 kb on - strand, within Sama_0320 at 387.926 kb on + strand, within Sama_0320 at 387.926 kb on + strand, within Sama_0320 at 387.926 kb on + strand, within Sama_0320 at 387.926 kb on + strand, within Sama_0320 at 387.927 kb on - strand, within Sama_0320 at 387.927 kb on - strand, within Sama_0320 at 387.945 kb on + strand, within Sama_0320 at 387.978 kb on + strand, within Sama_0320 at 387.979 kb on - strand, within Sama_0320 at 387.979 kb on - strand, within Sama_0320 at 387.979 kb on - strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.281 kb on + strand at 388.348 kb on + strand at 388.368 kb on + strand at 388.369 kb on - strand at 388.399 kb on + strand at 388.400 kb on - strand at 388.400 kb on - strand at 388.421 kb on + strand at 388.435 kb on + strand at 388.456 kb on + strand at 388.457 kb on - strand at 388.457 kb on - strand at 388.462 kb on - strand at 388.480 kb on + strand, within pyrE at 388.509 kb on - strand, within pyrE at 388.509 kb on - strand, within pyrE at 388.509 kb on - strand, within pyrE at 388.531 kb on + strand, within pyrE at 388.531 kb on + strand, within pyrE at 388.532 kb on - strand, within pyrE at 388.532 kb on - strand, within pyrE at 388.535 kb on - strand, within pyrE at 388.569 kb on - strand, within pyrE at 388.569 kb on - strand, within pyrE at 388.625 kb on + strand, within pyrE at 388.625 kb on + strand, within pyrE at 388.626 kb on - strand, within pyrE at 388.626 kb on - strand, within pyrE at 388.627 kb on + strand, within pyrE at 388.627 kb on + strand, within pyrE at 388.627 kb on + strand, within pyrE at 388.627 kb on + strand, within pyrE at 388.628 kb on - strand, within pyrE at 388.628 kb on - strand, within pyrE at 388.628 kb on - strand, within pyrE at 388.695 kb on - strand, within pyrE at 388.702 kb on + strand, within pyrE at 388.708 kb on + strand, within pyrE at 388.708 kb on + strand, within pyrE at 388.709 kb on - strand, within pyrE at 388.709 kb on - strand, within pyrE at 388.738 kb on + strand, within pyrE at 388.738 kb on + strand, within pyrE at 388.765 kb on + strand, within pyrE at 388.765 kb on + strand, within pyrE at 388.766 kb on - strand, within pyrE at 388.771 kb on + strand, within pyrE at 388.771 kb on + strand, within pyrE at 388.771 kb on + strand, within pyrE at 388.771 kb on + strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 389.022 kb on + strand at 389.030 kb on + strand at 389.030 kb on + strand at 389.051 kb on + strand at 389.051 kb on + strand at 389.051 kb on + strand at 389.052 kb on - strand at 389.052 kb on - strand at 389.064 kb on + strand at 389.064 kb on + strand at 389.064 kb on + strand at 389.071 kb on + strand at 389.100 kb on + strand at 389.101 kb on - strand at 389.101 kb on - strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.145 kb on - strand at 389.145 kb on - strand at 389.150 kb on + strand at 389.151 kb on - strand at 389.151 kb on - strand at 389.151 kb on - strand at 389.863 kb on - strand at 389.863 kb on - strand at 389.863 kb on - strand at 389.898 kb on - strand at 389.918 kb on + strand at 389.926 kb on - strand at 389.936 kb on + strand at 390.031 kb on + strand at 390.031 kb on + strand at 390.032 kb on - strand at 390.032 kb on - strand at 390.034 kb on + strand at 390.034 kb on + strand at 390.034 kb on + strand at 390.034 kb on + strand at 390.034 kb on + strand at 390.034 kb on + strand at 390.034 kb on + strand at 390.035 kb on - strand at 390.035 kb on - strand at 390.035 kb on - strand at 390.035 kb on - strand at 390.035 kb on - strand at 390.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 387,424 + -0.7 387,424 + -0.8 387,424 + -1.4 387,425 - -0.8 387,425 - -2.2 387,425 - -0.8 387,425 - -1.0 387,425 - -1.3 387,425 - -1.0 387,425 - -0.2 387,425 - -0.1 387,426 + +0.0 387,426 + -0.5 387,426 + -0.3 387,427 - -0.8 387,476 + -0.2 387,489 + -0.7 387,490 - +0.5 387,536 + +0.2 387,537 - +0.1 387,537 - -2.2 387,542 + +0.4 387,542 + +0.4 387,542 + -0.0 387,542 + +0.8 387,542 + -0.4 387,542 + +0.0 387,542 + +0.2 387,542 + +1.4 387,543 - +1.8 387,543 - +1.1 387,543 - -1.5 387,543 - -0.8 387,543 - -0.7 387,543 - -0.4 387,543 - +0.1 387,544 + +0.1 387,545 - -0.8 387,545 - -1.1 387,545 - -2.2 387,545 - -1.8 387,547 + -1.4 387,547 + -1.1 387,547 + +0.5 387,547 + +0.2 387,547 + -0.8 387,547 + -0.5 387,548 - -1.9 387,574 + -1.8 387,574 + -1.7 387,574 + +0.5 387,575 - -0.8 387,645 + -0.8 387,652 - -1.5 387,731 + Sama_0320 0.19 +0.1 387,731 + Sama_0320 0.19 -1.2 387,744 + Sama_0320 0.21 +0.1 387,779 - Sama_0320 0.26 -0.5 387,798 + Sama_0320 0.29 +0.2 387,799 - Sama_0320 0.29 -0.8 387,811 - Sama_0320 0.31 -0.8 387,902 + Sama_0320 0.43 -1.4 387,903 - Sama_0320 0.44 -1.2 387,926 + Sama_0320 0.47 -1.9 387,926 + Sama_0320 0.47 -0.4 387,926 + Sama_0320 0.47 -2.5 387,926 + Sama_0320 0.47 -0.2 387,927 - Sama_0320 0.47 -1.4 387,927 - Sama_0320 0.47 -1.0 387,945 + Sama_0320 0.49 +0.5 387,978 + Sama_0320 0.54 -0.5 387,979 - Sama_0320 0.54 -1.5 387,979 - Sama_0320 0.54 +0.2 387,979 - Sama_0320 0.54 +0.5 388,120 + Sama_0320 0.74 -1.5 388,120 + Sama_0320 0.74 -0.8 388,120 + Sama_0320 0.74 -2.4 388,120 + Sama_0320 0.74 -2.2 388,120 + Sama_0320 0.74 -1.8 388,281 + -0.5 388,348 + -0.2 388,368 + -0.8 388,369 - +0.1 388,399 + -0.4 388,400 - -1.1 388,400 - -0.7 388,421 + -2.5 388,435 + -1.4 388,456 + -1.8 388,457 - -1.4 388,457 - -2.1 388,462 - -0.4 388,480 + pyrE Sama_0321 0.11 -1.9 388,509 - pyrE Sama_0321 0.15 -2.7 388,509 - pyrE Sama_0321 0.15 -3.3 388,509 - pyrE Sama_0321 0.15 -1.7 388,531 + pyrE Sama_0321 0.19 -3.1 388,531 + pyrE Sama_0321 0.19 -0.5 388,532 - pyrE Sama_0321 0.19 -2.2 388,532 - pyrE Sama_0321 0.19 -2.4 388,535 - pyrE Sama_0321 0.19 -0.7 388,569 - pyrE Sama_0321 0.24 -2.9 388,569 - pyrE Sama_0321 0.24 -2.7 388,625 + pyrE Sama_0321 0.33 -2.2 388,625 + pyrE Sama_0321 0.33 -2.3 388,626 - pyrE Sama_0321 0.33 -1.2 388,626 - pyrE Sama_0321 0.33 -2.7 388,627 + pyrE Sama_0321 0.33 -1.2 388,627 + pyrE Sama_0321 0.33 -0.8 388,627 + pyrE Sama_0321 0.33 -2.2 388,627 + pyrE Sama_0321 0.33 -1.9 388,628 - pyrE Sama_0321 0.34 -0.8 388,628 - pyrE Sama_0321 0.34 -0.5 388,628 - pyrE Sama_0321 0.34 -0.8 388,695 - pyrE Sama_0321 0.44 -1.1 388,702 + pyrE Sama_0321 0.45 -3.3 388,708 + pyrE Sama_0321 0.46 -3.8 388,708 + pyrE Sama_0321 0.46 -1.2 388,709 - pyrE Sama_0321 0.46 -0.2 388,709 - pyrE Sama_0321 0.46 -1.7 388,738 + pyrE Sama_0321 0.51 -1.2 388,738 + pyrE Sama_0321 0.51 -1.9 388,765 + pyrE Sama_0321 0.55 -2.4 388,765 + pyrE Sama_0321 0.55 -3.0 388,766 - pyrE Sama_0321 0.55 -1.8 388,771 + pyrE Sama_0321 0.56 +0.5 388,771 + pyrE Sama_0321 0.56 -0.8 388,771 + pyrE Sama_0321 0.56 -0.2 388,771 + pyrE Sama_0321 0.56 -1.8 388,772 - pyrE Sama_0321 0.56 -0.2 388,772 - pyrE Sama_0321 0.56 +0.8 388,772 - pyrE Sama_0321 0.56 -2.7 388,772 - pyrE Sama_0321 0.56 -3.5 388,772 - pyrE Sama_0321 0.56 -0.2 388,772 - pyrE Sama_0321 0.56 -1.0 389,022 + -0.3 389,030 + -0.5 389,030 + -0.8 389,051 + +1.0 389,051 + -0.9 389,051 + -0.1 389,052 - -1.2 389,052 - +0.1 389,064 + -0.5 389,064 + -0.8 389,064 + -0.0 389,071 + +0.2 389,100 + +0.8 389,101 - +0.0 389,101 - +1.1 389,144 + -0.5 389,144 + +0.1 389,144 + -0.7 389,144 + +0.1 389,144 + -0.1 389,144 + +1.0 389,145 - -0.3 389,145 - +0.9 389,150 + -1.1 389,151 - -0.2 389,151 - -0.6 389,151 - -0.3 389,863 - -0.0 389,863 - +1.0 389,863 - +0.2 389,898 - +0.7 389,918 + +0.1 389,926 - +1.4 389,936 + -0.3 390,031 + +0.4 390,031 + +0.0 390,032 - +0.7 390,032 - -0.2 390,034 + +0.0 390,034 + -0.1 390,034 + +1.1 390,034 + -0.2 390,034 + -0.9 390,034 + +0.8 390,034 + +1.7 390,035 - -0.8 390,035 - -0.2 390,035 - -0.5 390,035 - +0.4 390,035 - +0.1 390,035 - -0.3
Or see this region's nucleotide sequence