Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0241 and Sama_0242 overlap by 4 nucleotides Sama_0242 and Sama_0243 overlap by 1 nucleotides Sama_0243 and Sama_0244 are separated by 1 nucleotides Sama_0244 and Sama_0245 are separated by 11 nucleotides Sama_0245 and Sama_0246 are separated by 310 nucleotides
Sama_0241: Sama_0241 - cytochrome c-type biogenesis protein CcmE (RefSeq), at 266,611 to 267,099
_0241
Sama_0242: Sama_0242 - heme exporter protein CcmD (RefSeq), at 267,096 to 267,296
_0242
Sama_0243: Sama_0243 - heme exporter protein CcmC (RefSeq), at 267,296 to 268,042
_0243
Sama_0244: Sama_0244 - heme exporter protein CcmB (RefSeq), at 268,044 to 268,730
_0244
Sama_0245: Sama_0245 - cytochrome c biogenesis protein CcmA (RefSeq), at 268,742 to 269,395
_0245
Sama_0246: Sama_0246 - cytochrome c (RefSeq), at 269,706 to 269,999
_0246
Position (kb) 268
269 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 267.084 kb on + strand at 267.084 kb on + strand at 267.189 kb on - strand, within Sama_0242 at 267.215 kb on + strand, within Sama_0242 at 267.222 kb on - strand, within Sama_0242 at 267.230 kb on - strand, within Sama_0242 at 267.244 kb on + strand, within Sama_0242 at 267.244 kb on + strand, within Sama_0242 at 267.245 kb on - strand, within Sama_0242 at 267.245 kb on - strand, within Sama_0242 at 267.245 kb on - strand, within Sama_0242 at 267.245 kb on - strand, within Sama_0242 at 267.245 kb on - strand, within Sama_0242 at 267.254 kb on - strand, within Sama_0242 at 267.270 kb on - strand, within Sama_0242 at 267.270 kb on - strand, within Sama_0242 at 267.302 kb on - strand at 267.418 kb on + strand, within Sama_0243 at 267.418 kb on + strand, within Sama_0243 at 267.418 kb on + strand, within Sama_0243 at 267.435 kb on + strand, within Sama_0243 at 267.519 kb on + strand, within Sama_0243 at 267.520 kb on - strand, within Sama_0243 at 267.606 kb on + strand, within Sama_0243 at 267.606 kb on + strand, within Sama_0243 at 267.627 kb on + strand, within Sama_0243 at 267.784 kb on + strand, within Sama_0243 at 267.805 kb on + strand, within Sama_0243 at 267.806 kb on - strand, within Sama_0243 at 267.829 kb on + strand, within Sama_0243 at 267.829 kb on + strand, within Sama_0243 at 267.830 kb on - strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.831 kb on + strand, within Sama_0243 at 267.832 kb on - strand, within Sama_0243 at 267.832 kb on - strand, within Sama_0243 at 267.868 kb on + strand, within Sama_0243 at 267.900 kb on + strand, within Sama_0243 at 267.901 kb on - strand, within Sama_0243 at 267.901 kb on - strand, within Sama_0243 at 267.901 kb on - strand, within Sama_0243 at 267.946 kb on + strand, within Sama_0243 at 267.978 kb on - strand at 267.978 kb on - strand at 267.992 kb on + strand at 268.000 kb on - strand at 268.028 kb on + strand at 268.029 kb on - strand at 268.043 kb on - strand at 268.045 kb on + strand at 268.045 kb on + strand at 268.046 kb on - strand at 268.046 kb on - strand at 268.049 kb on + strand at 268.050 kb on - strand at 268.050 kb on - strand at 268.119 kb on + strand, within Sama_0244 at 268.119 kb on + strand, within Sama_0244 at 268.120 kb on - strand, within Sama_0244 at 268.120 kb on - strand, within Sama_0244 at 268.120 kb on - strand, within Sama_0244 at 268.122 kb on + strand, within Sama_0244 at 268.142 kb on - strand, within Sama_0244 at 268.142 kb on - strand, within Sama_0244 at 268.147 kb on - strand, within Sama_0244 at 268.201 kb on + strand, within Sama_0244 at 268.202 kb on - strand, within Sama_0244 at 268.202 kb on - strand, within Sama_0244 at 268.202 kb on - strand, within Sama_0244 at 268.212 kb on + strand, within Sama_0244 at 268.213 kb on - strand, within Sama_0244 at 268.215 kb on - strand, within Sama_0244 at 268.216 kb on - strand, within Sama_0244 at 268.231 kb on - strand, within Sama_0244 at 268.241 kb on + strand, within Sama_0244 at 268.242 kb on - strand, within Sama_0244 at 268.242 kb on - strand, within Sama_0244 at 268.245 kb on + strand, within Sama_0244 at 268.245 kb on + strand, within Sama_0244 at 268.245 kb on + strand, within Sama_0244 at 268.246 kb on - strand, within Sama_0244 at 268.246 kb on - strand, within Sama_0244 at 268.269 kb on + strand, within Sama_0244 at 268.269 kb on + strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.270 kb on - strand, within Sama_0244 at 268.298 kb on + strand, within Sama_0244 at 268.330 kb on + strand, within Sama_0244 at 268.330 kb on + strand, within Sama_0244 at 268.331 kb on - strand, within Sama_0244 at 268.331 kb on - strand, within Sama_0244 at 268.331 kb on - strand, within Sama_0244 at 268.439 kb on - strand, within Sama_0244 at 268.439 kb on - strand, within Sama_0244 at 268.456 kb on - strand, within Sama_0244 at 268.539 kb on + strand, within Sama_0244 at 268.573 kb on - strand, within Sama_0244 at 268.573 kb on - strand, within Sama_0244 at 268.689 kb on + strand at 268.689 kb on + strand at 268.690 kb on - strand at 268.709 kb on + strand at 268.715 kb on + strand at 268.716 kb on - strand at 268.716 kb on - strand at 268.716 kb on - strand at 268.744 kb on - strand at 268.744 kb on - strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.745 kb on + strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.746 kb on - strand at 268.755 kb on + strand at 268.756 kb on - strand at 268.897 kb on + strand, within Sama_0245 at 268.980 kb on - strand, within Sama_0245 at 269.055 kb on + strand, within Sama_0245 at 269.070 kb on - strand, within Sama_0245 at 269.081 kb on - strand, within Sama_0245 at 269.133 kb on + strand, within Sama_0245 at 269.133 kb on + strand, within Sama_0245 at 269.134 kb on - strand, within Sama_0245 at 269.138 kb on + strand, within Sama_0245 at 269.138 kb on + strand, within Sama_0245 at 269.139 kb on - strand, within Sama_0245 at 269.139 kb on - strand, within Sama_0245 at 269.139 kb on - strand, within Sama_0245 at 269.139 kb on - strand, within Sama_0245 at 269.282 kb on - strand, within Sama_0245 at 269.361 kb on - strand at 269.387 kb on + strand at 269.388 kb on - strand at 269.405 kb on + strand at 269.405 kb on + strand at 269.406 kb on - strand at 269.406 kb on - strand at 269.406 kb on - strand at 269.406 kb on - strand at 269.416 kb on - strand at 269.430 kb on - strand at 269.432 kb on + strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.433 kb on - strand at 269.437 kb on + strand at 269.437 kb on + strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.438 kb on - strand at 269.456 kb on - strand at 269.466 kb on + strand at 269.466 kb on + strand at 269.466 kb on + strand at 269.467 kb on - strand at 269.467 kb on - strand at 269.467 kb on - strand at 269.471 kb on + strand at 269.471 kb on + strand at 269.471 kb on + strand at 269.472 kb on - strand at 269.472 kb on - strand at 269.472 kb on - strand at 269.472 kb on - strand at 269.475 kb on + strand at 269.475 kb on + strand at 269.476 kb on - strand at 269.476 kb on - strand at 269.500 kb on + strand at 269.501 kb on - strand at 269.501 kb on - strand at 269.501 kb on - strand at 269.529 kb on + strand at 269.539 kb on + strand at 269.540 kb on - strand at 269.541 kb on + strand at 269.542 kb on - strand at 269.542 kb on - strand at 269.542 kb on - strand at 269.560 kb on + strand at 269.560 kb on + strand at 269.625 kb on + strand at 269.625 kb on + strand at 269.625 kb on + strand at 269.626 kb on - strand at 269.626 kb on - strand at 269.633 kb on + strand at 269.642 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.679 kb on + strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.680 kb on - strand at 269.683 kb on + strand at 269.683 kb on + strand at 269.684 kb on - strand at 269.684 kb on - strand at 269.693 kb on - strand at 269.720 kb on - strand at 269.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 267,084 + -1.1 267,084 + -1.5 267,189 - Sama_0242 0.46 -1.1 267,215 + Sama_0242 0.59 -0.7 267,222 - Sama_0242 0.63 -3.2 267,230 - Sama_0242 0.67 -0.1 267,244 + Sama_0242 0.74 -1.7 267,244 + Sama_0242 0.74 -0.7 267,245 - Sama_0242 0.74 -1.9 267,245 - Sama_0242 0.74 -2.8 267,245 - Sama_0242 0.74 -0.5 267,245 - Sama_0242 0.74 -1.7 267,245 - Sama_0242 0.74 -1.7 267,254 - Sama_0242 0.79 -0.5 267,270 - Sama_0242 0.87 -1.9 267,270 - Sama_0242 0.87 -2.3 267,302 - -1.3 267,418 + Sama_0243 0.16 -1.9 267,418 + Sama_0243 0.16 -0.7 267,418 + Sama_0243 0.16 -1.7 267,435 + Sama_0243 0.19 -1.5 267,519 + Sama_0243 0.30 +0.9 267,520 - Sama_0243 0.30 -0.7 267,606 + Sama_0243 0.41 -2.5 267,606 + Sama_0243 0.41 +0.4 267,627 + Sama_0243 0.44 -0.1 267,784 + Sama_0243 0.65 -1.1 267,805 + Sama_0243 0.68 +0.9 267,806 - Sama_0243 0.68 +0.6 267,829 + Sama_0243 0.71 -0.1 267,829 + Sama_0243 0.71 +0.3 267,830 - Sama_0243 0.71 +2.9 267,831 + Sama_0243 0.72 +0.5 267,831 + Sama_0243 0.72 -1.1 267,831 + Sama_0243 0.72 -1.9 267,831 + Sama_0243 0.72 -2.5 267,831 + Sama_0243 0.72 -0.7 267,831 + Sama_0243 0.72 -0.7 267,831 + Sama_0243 0.72 -1.3 267,832 - Sama_0243 0.72 -1.9 267,832 - Sama_0243 0.72 -0.1 267,868 + Sama_0243 0.77 -2.1 267,900 + Sama_0243 0.81 -1.9 267,901 - Sama_0243 0.81 -1.1 267,901 - Sama_0243 0.81 -0.6 267,901 - Sama_0243 0.81 -1.5 267,946 + Sama_0243 0.87 -0.7 267,978 - -1.5 267,978 - -0.9 267,992 + +0.3 268,000 - -1.7 268,028 + -0.7 268,029 - -2.3 268,043 - +1.1 268,045 + -0.5 268,045 + -1.8 268,046 - +0.9 268,046 - -0.3 268,049 + -1.7 268,050 - -0.1 268,050 - -2.0 268,119 + Sama_0244 0.11 -0.1 268,119 + Sama_0244 0.11 -0.7 268,120 - Sama_0244 0.11 -1.7 268,120 - Sama_0244 0.11 -2.0 268,120 - Sama_0244 0.11 +0.9 268,122 + Sama_0244 0.11 -0.7 268,142 - Sama_0244 0.14 -0.7 268,142 - Sama_0244 0.14 -1.1 268,147 - Sama_0244 0.15 -1.3 268,201 + Sama_0244 0.23 -1.1 268,202 - Sama_0244 0.23 -2.0 268,202 - Sama_0244 0.23 -2.6 268,202 - Sama_0244 0.23 -1.5 268,212 + Sama_0244 0.24 -2.0 268,213 - Sama_0244 0.25 -2.3 268,215 - Sama_0244 0.25 +0.1 268,216 - Sama_0244 0.25 -0.7 268,231 - Sama_0244 0.27 -0.1 268,241 + Sama_0244 0.29 -2.1 268,242 - Sama_0244 0.29 -1.5 268,242 - Sama_0244 0.29 +0.0 268,245 + Sama_0244 0.29 -0.1 268,245 + Sama_0244 0.29 -2.3 268,245 + Sama_0244 0.29 -1.7 268,246 - Sama_0244 0.29 -1.5 268,246 - Sama_0244 0.29 -3.1 268,269 + Sama_0244 0.33 -1.5 268,269 + Sama_0244 0.33 -1.1 268,270 - Sama_0244 0.33 -2.3 268,270 - Sama_0244 0.33 -2.7 268,270 - Sama_0244 0.33 -2.0 268,270 - Sama_0244 0.33 +0.1 268,270 - Sama_0244 0.33 -2.6 268,298 + Sama_0244 0.37 -0.7 268,330 + Sama_0244 0.42 -1.1 268,330 + Sama_0244 0.42 -1.5 268,331 - Sama_0244 0.42 -3.5 268,331 - Sama_0244 0.42 -1.3 268,331 - Sama_0244 0.42 -1.6 268,439 - Sama_0244 0.57 +0.9 268,439 - Sama_0244 0.57 +0.4 268,456 - Sama_0244 0.60 -0.7 268,539 + Sama_0244 0.72 -2.1 268,573 - Sama_0244 0.77 -1.6 268,573 - Sama_0244 0.77 -2.5 268,689 + -0.7 268,689 + -1.5 268,690 - -0.7 268,709 + +0.4 268,715 + -1.1 268,716 - +0.9 268,716 - -2.1 268,716 - -1.7 268,744 - -2.0 268,744 - -0.8 268,745 + -0.7 268,745 + -2.8 268,745 + -2.3 268,745 + -1.1 268,745 + -1.7 268,745 + -1.7 268,745 + -1.5 268,746 - -0.7 268,746 - -0.7 268,746 - -0.7 268,746 - -1.5 268,746 - -1.7 268,746 - -1.1 268,755 + -0.1 268,756 - -0.5 268,897 + Sama_0245 0.24 -1.7 268,980 - Sama_0245 0.36 -0.7 269,055 + Sama_0245 0.48 -2.9 269,070 - Sama_0245 0.50 -2.7 269,081 - Sama_0245 0.52 -1.5 269,133 + Sama_0245 0.60 -2.1 269,133 + Sama_0245 0.60 -2.3 269,134 - Sama_0245 0.60 -1.3 269,138 + Sama_0245 0.61 -2.6 269,138 + Sama_0245 0.61 -2.8 269,139 - Sama_0245 0.61 -1.5 269,139 - Sama_0245 0.61 -0.5 269,139 - Sama_0245 0.61 -1.3 269,139 - Sama_0245 0.61 +0.2 269,282 - Sama_0245 0.83 -2.3 269,361 - +0.6 269,387 + -1.1 269,388 - -1.5 269,405 + -0.7 269,405 + -2.1 269,406 - -0.0 269,406 - +0.5 269,406 - -0.3 269,406 - -0.7 269,416 - +0.2 269,430 - +0.4 269,432 + +0.1 269,433 - +0.7 269,433 - -2.5 269,433 - -0.5 269,433 - -0.4 269,433 - -1.2 269,433 - +0.2 269,433 - -0.3 269,437 + +0.7 269,437 + -1.5 269,438 - -0.1 269,438 - -0.0 269,438 - +0.3 269,438 - +2.1 269,438 - -0.0 269,438 - -0.7 269,438 - -1.7 269,438 - +0.6 269,456 - +0.4 269,466 + +0.1 269,466 + +1.0 269,466 + -0.7 269,467 - +0.3 269,467 - +1.1 269,467 - +0.4 269,471 + +2.0 269,471 + +1.0 269,471 + +0.1 269,472 - -0.5 269,472 - -0.1 269,472 - +1.4 269,472 - -0.8 269,475 + +0.0 269,475 + -0.3 269,476 - -0.2 269,476 - -1.0 269,500 + -0.1 269,501 - -0.0 269,501 - +0.3 269,501 - +0.1 269,529 + +0.5 269,539 + +0.9 269,540 - -1.0 269,541 + +0.1 269,542 - +0.3 269,542 - +0.1 269,542 - +0.5 269,560 + +0.3 269,560 + +0.3 269,625 + +0.7 269,625 + +0.6 269,625 + -0.2 269,626 - -0.1 269,626 - +0.7 269,633 + +2.5 269,642 + +0.9 269,679 + +1.5 269,679 + +0.4 269,679 + +0.7 269,679 + +0.7 269,679 + -0.4 269,679 + +0.0 269,679 + -0.1 269,679 + +0.4 269,679 + +0.5 269,679 + +0.7 269,679 + +0.6 269,679 + -1.0 269,680 - +0.3 269,680 - -0.8 269,680 - -0.9 269,680 - +1.6 269,680 - -0.5 269,680 - +0.9 269,680 - +0.2 269,680 - -3.0 269,680 - -0.7 269,683 + +0.6 269,683 + +0.1 269,684 - +0.2 269,684 - -0.9 269,693 - -1.0 269,720 - -0.4 269,720 - -1.5
Or see this region's nucleotide sequence