Experiment: Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0170 and Sama_0171 are separated by 3 nucleotides Sama_0171 and Sama_0172 are separated by 37 nucleotides Sama_0172 and Sama_0173 are separated by 12 nucleotides
Sama_0170: Sama_0170 - general secretion pathway protein E (RefSeq), at 200,369 to 201,931
_0170
Sama_0171: Sama_0171 - general secretion pathway protein F (RefSeq), at 201,935 to 203,158
_0171
Sama_0172: Sama_0172 - general secretion pathway protein G (RefSeq), at 203,196 to 203,627
_0172
Sama_0173: Sama_0173 - general secretion pathway protein H (RefSeq), at 203,640 to 204,209
_0173
Position (kb) 201
202
203
204 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 201.026 kb on - strand, within Sama_0170 at 201.119 kb on - strand, within Sama_0170 at 201.311 kb on + strand, within Sama_0170 at 201.325 kb on + strand, within Sama_0170 at 201.326 kb on - strand, within Sama_0170 at 201.327 kb on + strand, within Sama_0170 at 201.328 kb on - strand, within Sama_0170 at 201.470 kb on - strand, within Sama_0170 at 201.521 kb on - strand, within Sama_0170 at 201.521 kb on - strand, within Sama_0170 at 201.582 kb on + strand, within Sama_0170 at 201.600 kb on + strand, within Sama_0170 at 201.622 kb on + strand, within Sama_0170 at 201.790 kb on + strand at 201.791 kb on - strand at 201.907 kb on - strand at 201.907 kb on - strand at 201.929 kb on + strand at 202.283 kb on + strand, within Sama_0171 at 202.284 kb on - strand, within Sama_0171 at 202.343 kb on - strand, within Sama_0171 at 202.343 kb on - strand, within Sama_0171 at 202.571 kb on - strand, within Sama_0171 at 202.608 kb on - strand, within Sama_0171 at 202.617 kb on + strand, within Sama_0171 at 202.843 kb on - strand, within Sama_0171 at 202.946 kb on + strand, within Sama_0171 at 202.946 kb on + strand, within Sama_0171 at 202.947 kb on - strand, within Sama_0171 at 202.949 kb on - strand, within Sama_0171 at 203.101 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.156 kb on + strand at 203.160 kb on + strand at 203.160 kb on + strand at 203.160 kb on + strand at 203.178 kb on + strand at 203.178 kb on + strand at 203.178 kb on + strand at 203.179 kb on - strand at 203.419 kb on + strand, within Sama_0172 at 203.453 kb on + strand, within Sama_0172 at 203.512 kb on - strand, within Sama_0172 at 203.571 kb on - strand, within Sama_0172 at 203.594 kb on + strand at 203.620 kb on - strand at 203.625 kb on + strand at 203.625 kb on + strand at 203.625 kb on + strand at 203.626 kb on - strand at 203.665 kb on + strand at 203.687 kb on - strand at 203.849 kb on + strand, within Sama_0173 at 203.850 kb on - strand, within Sama_0173 at 203.853 kb on + strand, within Sama_0173 at 203.854 kb on - strand, within Sama_0173 at 203.859 kb on + strand, within Sama_0173 at 203.860 kb on - strand, within Sama_0173 at 203.868 kb on + strand, within Sama_0173 at 203.942 kb on + strand, within Sama_0173 at 204.015 kb on - strand, within Sama_0173 at 204.070 kb on + strand, within Sama_0173
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.0125 mg/ml remove 201,026 - Sama_0170 0.42 -2.9 201,119 - Sama_0170 0.48 -0.7 201,311 + Sama_0170 0.60 -2.1 201,325 + Sama_0170 0.61 -0.1 201,326 - Sama_0170 0.61 -0.8 201,327 + Sama_0170 0.61 -0.7 201,328 - Sama_0170 0.61 -1.9 201,470 - Sama_0170 0.70 -0.7 201,521 - Sama_0170 0.74 +0.1 201,521 - Sama_0170 0.74 -0.7 201,582 + Sama_0170 0.78 -0.1 201,600 + Sama_0170 0.79 -0.7 201,622 + Sama_0170 0.80 +1.9 201,790 + +0.9 201,791 - +0.1 201,907 - +0.1 201,907 - +0.9 201,929 + -0.1 202,283 + Sama_0171 0.28 +0.6 202,284 - Sama_0171 0.29 -0.9 202,343 - Sama_0171 0.33 -1.9 202,343 - Sama_0171 0.33 -1.1 202,571 - Sama_0171 0.52 -1.1 202,608 - Sama_0171 0.55 +0.3 202,617 + Sama_0171 0.56 +2.2 202,843 - Sama_0171 0.74 -2.1 202,946 + Sama_0171 0.83 -1.9 202,946 + Sama_0171 0.83 +0.6 202,947 - Sama_0171 0.83 -1.7 202,949 - Sama_0171 0.83 -2.5 203,101 + -0.7 203,156 + -0.1 203,156 + +0.6 203,156 + +0.5 203,156 + -1.2 203,156 + +0.2 203,156 + -0.5 203,160 + -0.0 203,160 + +0.8 203,160 + +0.0 203,178 + -0.2 203,178 + -0.4 203,178 + +0.5 203,179 - +0.9 203,419 + Sama_0172 0.52 -1.5 203,453 + Sama_0172 0.59 -1.5 203,512 - Sama_0172 0.73 -1.1 203,571 - Sama_0172 0.87 -0.7 203,594 + -2.5 203,620 - +0.9 203,625 + -0.7 203,625 + +0.9 203,625 + +0.8 203,626 - -0.7 203,665 + -1.6 203,687 - -0.7 203,849 + Sama_0173 0.37 -1.9 203,850 - Sama_0173 0.37 +1.6 203,853 + Sama_0173 0.37 -0.4 203,854 - Sama_0173 0.38 -1.7 203,859 + Sama_0173 0.38 +0.9 203,860 - Sama_0173 0.39 -0.7 203,868 + Sama_0173 0.40 +0.3 203,942 + Sama_0173 0.53 -1.9 204,015 - Sama_0173 0.66 -0.7 204,070 + Sama_0173 0.75 -0.7
Or see this region's nucleotide sequence