Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3933 and SO3934 are separated by 71 nucleotides SO3934 and vacB are separated by 0 nucleotides vacB and motX are separated by 40 nucleotides motX and purA are separated by 143 nucleotides
SO3933: SO3933 - membrane protein, putative (NCBI ptt file), at 4,077,285 to 4,078,709
SO3933
SO3934: SO3934 - RNA methyltransferase, TrmH family, group 3 (NCBI ptt file), at 4,078,781 to 4,079,521
SO3934
SO3935: vacB - ribonuclease R (NCBI ptt file), at 4,079,522 to 4,081,948
vacB
SO3936: motX - sodium-type flagellar protein MotX (NCBI ptt file), at 4,081,989 to 4,082,609
motX
SO3937: purA - adenylosuccinate synthetase (NCBI ptt file), at 4,082,753 to 4,084,048
purA
Position (kb)
4079
4080
4081
4082 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4078.811 kb on - strand at 4078.870 kb on - strand, within SO3934 at 4078.907 kb on + strand, within SO3934 at 4078.907 kb on + strand, within SO3934 at 4078.915 kb on - strand, within SO3934 at 4078.915 kb on - strand, within SO3934 at 4078.915 kb on - strand, within SO3934 at 4078.915 kb on - strand, within SO3934 at 4078.921 kb on - strand, within SO3934 at 4079.124 kb on + strand, within SO3934 at 4079.150 kb on - strand, within SO3934 at 4079.150 kb on - strand, within SO3934 at 4079.193 kb on - strand, within SO3934 at 4079.200 kb on + strand, within SO3934 at 4079.318 kb on - strand, within SO3934 at 4079.325 kb on - strand, within SO3934 at 4079.468 kb on - strand at 4079.485 kb on - strand at 4079.485 kb on - strand at 4079.640 kb on + strand at 4079.648 kb on - strand at 4079.742 kb on + strand at 4079.750 kb on - strand at 4079.800 kb on + strand, within vacB at 4079.804 kb on - strand, within vacB at 4079.808 kb on - strand, within vacB at 4079.829 kb on - strand, within vacB at 4079.829 kb on - strand, within vacB at 4079.928 kb on - strand, within vacB at 4079.928 kb on - strand, within vacB at 4080.081 kb on + strand, within vacB at 4080.180 kb on + strand, within vacB at 4080.430 kb on + strand, within vacB at 4080.430 kb on + strand, within vacB at 4080.438 kb on - strand, within vacB at 4080.438 kb on - strand, within vacB at 4080.516 kb on + strand, within vacB at 4080.797 kb on + strand, within vacB at 4080.891 kb on + strand, within vacB at 4080.952 kb on + strand, within vacB at 4080.960 kb on - strand, within vacB at 4081.010 kb on + strand, within vacB at 4081.081 kb on - strand, within vacB at 4081.085 kb on - strand, within vacB at 4081.249 kb on - strand, within vacB at 4081.553 kb on - strand, within vacB at 4081.655 kb on - strand, within vacB at 4081.676 kb on + strand, within vacB at 4081.789 kb on - strand at 4081.814 kb on + strand at 4082.011 kb on + strand at 4082.050 kb on + strand at 4082.058 kb on - strand, within motX at 4082.121 kb on + strand, within motX at 4082.121 kb on + strand, within motX at 4082.142 kb on + strand, within motX at 4082.149 kb on - strand, within motX at 4082.151 kb on + strand, within motX at 4082.151 kb on + strand, within motX at 4082.159 kb on - strand, within motX at 4082.308 kb on - strand, within motX at 4082.313 kb on - strand, within motX at 4082.318 kb on - strand, within motX at 4082.331 kb on + strand, within motX at 4082.337 kb on + strand, within motX at 4082.505 kb on - strand, within motX at 4082.560 kb on - strand at 4082.572 kb on + strand at 4082.599 kb on + strand at 4082.791 kb on + strand at 4082.792 kb on + strand at 4082.937 kb on + strand, within purA at 4082.947 kb on + strand, within purA
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 4,078,811 - -0.5 4,078,870 - SO3934 0.12 -0.7 4,078,907 + SO3934 0.17 +0.2 4,078,907 + SO3934 0.17 -0.1 4,078,915 - SO3934 0.18 -0.8 4,078,915 - SO3934 0.18 +0.2 4,078,915 - SO3934 0.18 -0.5 4,078,915 - SO3934 0.18 +0.5 4,078,921 - SO3934 0.19 -0.4 4,079,124 + SO3934 0.46 -0.0 4,079,150 - SO3934 0.50 -1.5 4,079,150 - SO3934 0.50 +0.0 4,079,193 - SO3934 0.56 -0.9 4,079,200 + SO3934 0.57 -1.2 4,079,318 - SO3934 0.72 +1.5 4,079,325 - SO3934 0.73 -0.5 4,079,468 - -0.8 4,079,485 - -0.9 4,079,485 - +0.6 4,079,640 + -0.4 4,079,648 - -0.2 4,079,742 + -0.4 4,079,750 - +0.8 4,079,800 + vacB SO3935 0.11 -2.5 4,079,804 - vacB SO3935 0.12 +0.6 4,079,808 - vacB SO3935 0.12 +0.5 4,079,829 - vacB SO3935 0.13 -2.8 4,079,829 - vacB SO3935 0.13 -2.2 4,079,928 - vacB SO3935 0.17 -0.9 4,079,928 - vacB SO3935 0.17 -1.2 4,080,081 + vacB SO3935 0.23 -2.7 4,080,180 + vacB SO3935 0.27 -0.9 4,080,430 + vacB SO3935 0.37 -1.6 4,080,430 + vacB SO3935 0.37 -1.7 4,080,438 - vacB SO3935 0.38 -2.4 4,080,438 - vacB SO3935 0.38 -1.8 4,080,516 + vacB SO3935 0.41 -0.4 4,080,797 + vacB SO3935 0.53 -0.9 4,080,891 + vacB SO3935 0.56 -0.2 4,080,952 + vacB SO3935 0.59 -1.4 4,080,960 - vacB SO3935 0.59 -2.1 4,081,010 + vacB SO3935 0.61 -2.5 4,081,081 - vacB SO3935 0.64 -2.8 4,081,085 - vacB SO3935 0.64 -0.2 4,081,249 - vacB SO3935 0.71 -1.9 4,081,553 - vacB SO3935 0.84 -0.4 4,081,655 - vacB SO3935 0.88 -1.6 4,081,676 + vacB SO3935 0.89 -2.2 4,081,789 - -1.2 4,081,814 + -2.5 4,082,011 + -1.6 4,082,050 + -2.9 4,082,058 - motX SO3936 0.11 -1.5 4,082,121 + motX SO3936 0.21 -2.4 4,082,121 + motX SO3936 0.21 -3.8 4,082,142 + motX SO3936 0.25 -2.7 4,082,149 - motX SO3936 0.26 -2.1 4,082,151 + motX SO3936 0.26 -2.8 4,082,151 + motX SO3936 0.26 -2.2 4,082,159 - motX SO3936 0.27 -0.8 4,082,308 - motX SO3936 0.51 -2.5 4,082,313 - motX SO3936 0.52 -1.0 4,082,318 - motX SO3936 0.53 -1.5 4,082,331 + motX SO3936 0.55 -2.5 4,082,337 + motX SO3936 0.56 -2.5 4,082,505 - motX SO3936 0.83 -1.6 4,082,560 - -1.5 4,082,572 + -2.2 4,082,599 + -4.4 4,082,791 + -0.9 4,082,792 + +0.5 4,082,937 + purA SO3937 0.14 +0.0 4,082,947 + purA SO3937 0.15 -0.1
Or see this region's nucleotide sequence