Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliS and SO3234 are separated by 6 nucleotides SO3234 and fliD are separated by 28 nucleotides fliD and flaG are separated by 42 nucleotides flaG and SO3237 are separated by 156 nucleotides
SO3233: fliS - flagellar protein FliS (NCBI ptt file), at 3,373,086 to 3,373,496
fliS
SO3234: SO3234 - hypothetical protein (NCBI ptt file), at 3,373,503 to 3,373,823
SO3234
SO3235: fliD - flagellar hook-associated protein FliD (NCBI ptt file), at 3,373,852 to 3,375,222
fliD
SO3236: flaG - flagellin FlaG (NCBI ptt file), at 3,375,265 to 3,375,624
flaG
SO3237: SO3237 - flagellin (NCBI ptt file), at 3,375,781 to 3,376,599
SO3237
Position (kb)
3373
3374
3375
3376 Strain fitness (log2 ratio)
-2
-1
0
1 at 3372.884 kb on - strand at 3372.901 kb on - strand at 3372.917 kb on + strand at 3373.116 kb on + strand at 3373.116 kb on + strand at 3373.116 kb on + strand at 3373.136 kb on + strand, within fliS at 3373.145 kb on - strand, within fliS at 3373.156 kb on - strand, within fliS at 3373.361 kb on + strand, within fliS at 3373.370 kb on + strand, within fliS at 3373.377 kb on + strand, within fliS at 3373.377 kb on + strand, within fliS at 3373.385 kb on - strand, within fliS at 3373.385 kb on - strand, within fliS at 3373.385 kb on - strand, within fliS at 3373.389 kb on + strand, within fliS at 3373.389 kb on + strand, within fliS at 3373.397 kb on - strand, within fliS at 3373.400 kb on - strand, within fliS at 3373.426 kb on - strand, within fliS at 3373.500 kb on + strand at 3373.500 kb on - strand at 3373.539 kb on - strand, within SO3234 at 3373.624 kb on + strand, within SO3234 at 3373.684 kb on - strand, within SO3234 at 3373.711 kb on - strand, within SO3234 at 3373.711 kb on - strand, within SO3234 at 3373.733 kb on + strand, within SO3234 at 3373.749 kb on + strand, within SO3234 at 3373.775 kb on + strand, within SO3234 at 3373.775 kb on + strand, within SO3234 at 3373.783 kb on - strand, within SO3234 at 3373.801 kb on - strand at 3374.020 kb on + strand, within fliD at 3374.028 kb on - strand, within fliD at 3374.036 kb on - strand, within fliD at 3374.178 kb on + strand, within fliD at 3374.323 kb on + strand, within fliD at 3374.331 kb on - strand, within fliD at 3374.371 kb on - strand, within fliD at 3374.468 kb on + strand, within fliD at 3374.642 kb on - strand, within fliD at 3374.642 kb on - strand, within fliD at 3374.698 kb on + strand, within fliD at 3374.740 kb on + strand, within fliD at 3374.977 kb on + strand, within fliD at 3374.982 kb on + strand, within fliD at 3375.121 kb on + strand at 3375.184 kb on + strand at 3375.196 kb on + strand at 3375.262 kb on + strand at 3375.629 kb on - strand at 3375.715 kb on + strand at 3375.966 kb on - strand, within SO3237 at 3375.981 kb on - strand, within SO3237 at 3375.983 kb on + strand, within SO3237 at 3376.122 kb on + strand, within SO3237 at 3376.196 kb on + strand, within SO3237
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 3,372,884 - -0.2 3,372,901 - +0.6 3,372,917 + -1.5 3,373,116 + +1.2 3,373,116 + -0.4 3,373,116 + -0.9 3,373,136 + fliS SO3233 0.12 -1.6 3,373,145 - fliS SO3233 0.14 -1.7 3,373,156 - fliS SO3233 0.17 -1.6 3,373,361 + fliS SO3233 0.67 -1.0 3,373,370 + fliS SO3233 0.69 -0.7 3,373,377 + fliS SO3233 0.71 -2.0 3,373,377 + fliS SO3233 0.71 -1.0 3,373,385 - fliS SO3233 0.73 -0.6 3,373,385 - fliS SO3233 0.73 -2.0 3,373,385 - fliS SO3233 0.73 -2.2 3,373,389 + fliS SO3233 0.74 -0.6 3,373,389 + fliS SO3233 0.74 -0.4 3,373,397 - fliS SO3233 0.76 -1.0 3,373,400 - fliS SO3233 0.76 -0.9 3,373,426 - fliS SO3233 0.83 -1.4 3,373,500 + -0.8 3,373,500 - +1.3 3,373,539 - SO3234 0.11 -0.1 3,373,624 + SO3234 0.38 -0.4 3,373,684 - SO3234 0.56 -1.0 3,373,711 - SO3234 0.65 +0.4 3,373,711 - SO3234 0.65 -1.6 3,373,733 + SO3234 0.72 -0.8 3,373,749 + SO3234 0.77 -2.3 3,373,775 + SO3234 0.85 -1.0 3,373,775 + SO3234 0.85 -0.2 3,373,783 - SO3234 0.87 -0.5 3,373,801 - +0.3 3,374,020 + fliD SO3235 0.12 -1.0 3,374,028 - fliD SO3235 0.13 -1.7 3,374,036 - fliD SO3235 0.13 -1.1 3,374,178 + fliD SO3235 0.24 -1.1 3,374,323 + fliD SO3235 0.34 -0.6 3,374,331 - fliD SO3235 0.35 -1.1 3,374,371 - fliD SO3235 0.38 -1.6 3,374,468 + fliD SO3235 0.45 -1.0 3,374,642 - fliD SO3235 0.58 -1.3 3,374,642 - fliD SO3235 0.58 -0.4 3,374,698 + fliD SO3235 0.62 +0.0 3,374,740 + fliD SO3235 0.65 -1.4 3,374,977 + fliD SO3235 0.82 -0.5 3,374,982 + fliD SO3235 0.82 -0.3 3,375,121 + -1.7 3,375,184 + +0.0 3,375,196 + -0.6 3,375,262 + -1.6 3,375,629 - +0.1 3,375,715 + -0.7 3,375,966 - SO3237 0.23 -1.2 3,375,981 - SO3237 0.24 -2.0 3,375,983 + SO3237 0.25 -2.4 3,376,122 + SO3237 0.42 -1.1 3,376,196 + SO3237 0.51 -0.8
Or see this region's nucleotide sequence