Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliL are separated by 39 nucleotides fliL and fliK are separated by 82 nucleotides fliK and fliJ are separated by 143 nucleotides fliJ and fliI are separated by 11 nucleotides
SO3221: fliM - flagellar motor switch protein FliM (NCBI ptt file), at 3,359,428 to 3,360,459
fliM
SO3222: fliL - flagellar protein FliL (NCBI ptt file), at 3,360,499 to 3,361,023
fliL
SO3223: fliK - flagellar hook-length control protein FliK (NCBI ptt file), at 3,361,106 to 3,362,689
fliK
SO3224: fliJ - flagellar protein FliJ (NCBI ptt file), at 3,362,833 to 3,363,276
fliJ
SO3225: fliI - flagellum-specific ATP synthase FliI (NCBI ptt file), at 3,363,288 to 3,364,625
fliI
Position (kb)
3361
3362
3363 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3360.157 kb on + strand, within fliM at 3360.237 kb on - strand, within fliM at 3360.327 kb on + strand, within fliM at 3360.415 kb on + strand at 3360.426 kb on + strand at 3360.653 kb on - strand, within fliL at 3360.653 kb on - strand, within fliL at 3360.772 kb on - strand, within fliL at 3360.803 kb on - strand, within fliL at 3360.858 kb on - strand, within fliL at 3360.947 kb on - strand, within fliL at 3361.019 kb on + strand at 3361.024 kb on + strand at 3361.027 kb on - strand at 3361.058 kb on + strand at 3361.235 kb on + strand at 3361.251 kb on + strand at 3361.259 kb on - strand at 3361.259 kb on - strand at 3361.346 kb on + strand, within fliK at 3361.623 kb on + strand, within fliK at 3361.638 kb on + strand, within fliK at 3361.638 kb on + strand, within fliK at 3361.638 kb on + strand, within fliK at 3361.640 kb on + strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.804 kb on - strand, within fliK at 3361.806 kb on + strand, within fliK at 3361.918 kb on + strand, within fliK at 3362.053 kb on + strand, within fliK at 3362.086 kb on + strand, within fliK at 3362.094 kb on - strand, within fliK at 3362.103 kb on + strand, within fliK at 3362.244 kb on - strand, within fliK at 3362.248 kb on + strand, within fliK at 3362.263 kb on - strand, within fliK at 3362.496 kb on - strand, within fliK at 3362.695 kb on + strand at 3362.980 kb on - strand, within fliJ at 3363.084 kb on + strand, within fliJ at 3363.092 kb on + strand, within fliJ at 3363.100 kb on - strand, within fliJ at 3363.100 kb on - strand, within fliJ at 3363.338 kb on + strand at 3363.502 kb on + strand, within fliI at 3363.502 kb on + strand, within fliI at 3363.510 kb on - strand, within fliI at 3363.510 kb on - strand, within fliI at 3363.510 kb on - strand, within fliI at 3363.510 kb on - strand, within fliI at 3363.608 kb on + strand, within fliI at 3363.613 kb on - strand, within fliI
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 3,360,157 + fliM SO3221 0.71 -0.2 3,360,237 - fliM SO3221 0.78 +0.4 3,360,327 + fliM SO3221 0.87 -1.0 3,360,415 + -0.5 3,360,426 + -1.1 3,360,653 - fliL SO3222 0.29 -0.3 3,360,653 - fliL SO3222 0.29 -0.5 3,360,772 - fliL SO3222 0.52 +0.2 3,360,803 - fliL SO3222 0.58 +0.6 3,360,858 - fliL SO3222 0.68 -1.2 3,360,947 - fliL SO3222 0.85 -0.4 3,361,019 + -1.6 3,361,024 + -1.0 3,361,027 - +0.9 3,361,058 + -2.4 3,361,235 + -0.4 3,361,251 + -0.1 3,361,259 - -1.2 3,361,259 - -0.8 3,361,346 + fliK SO3223 0.15 -0.9 3,361,623 + fliK SO3223 0.33 -1.2 3,361,638 + fliK SO3223 0.34 -1.2 3,361,638 + fliK SO3223 0.34 -0.8 3,361,638 + fliK SO3223 0.34 -3.0 3,361,640 + fliK SO3223 0.34 -1.1 3,361,646 - fliK SO3223 0.34 -1.2 3,361,646 - fliK SO3223 0.34 +0.5 3,361,646 - fliK SO3223 0.34 -0.8 3,361,646 - fliK SO3223 0.34 -1.1 3,361,646 - fliK SO3223 0.34 -0.6 3,361,804 - fliK SO3223 0.44 +0.0 3,361,806 + fliK SO3223 0.44 -0.8 3,361,918 + fliK SO3223 0.51 -2.8 3,362,053 + fliK SO3223 0.60 -1.0 3,362,086 + fliK SO3223 0.62 -0.2 3,362,094 - fliK SO3223 0.62 -1.9 3,362,103 + fliK SO3223 0.63 -0.8 3,362,244 - fliK SO3223 0.72 -2.4 3,362,248 + fliK SO3223 0.72 -1.2 3,362,263 - fliK SO3223 0.73 -2.2 3,362,496 - fliK SO3223 0.88 -0.9 3,362,695 + -2.3 3,362,980 - fliJ SO3224 0.33 -0.2 3,363,084 + fliJ SO3224 0.57 -0.5 3,363,092 + fliJ SO3224 0.58 -2.1 3,363,100 - fliJ SO3224 0.60 -1.1 3,363,100 - fliJ SO3224 0.60 -1.0 3,363,338 + -1.6 3,363,502 + fliI SO3225 0.16 -1.1 3,363,502 + fliI SO3225 0.16 -1.5 3,363,510 - fliI SO3225 0.17 -1.2 3,363,510 - fliI SO3225 0.17 -0.5 3,363,510 - fliI SO3225 0.17 -1.2 3,363,510 - fliI SO3225 0.17 -2.7 3,363,608 + fliI SO3225 0.24 -0.7 3,363,613 - fliI SO3225 0.24 -2.4
Or see this region's nucleotide sequence