Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apt and dnaX are separated by 131 nucleotides dnaX and SO2014 are separated by 229 nucleotides SO2014 and recR are separated by 12 nucleotides
SO2012: apt - adenine phosphoribosyltransferase (NCBI ptt file), at 2,109,294 to 2,109,839
apt
SO2013: dnaX - DNA polymerase III, gamma and tau subunits (NCBI ptt file), at 2,109,971 to 2,112,979
dnaX
SO2014: SO2014 - conserved hypothetical protein TIGR00103 (NCBI ptt file), at 2,113,209 to 2,113,538
SO2014
SO2015: recR - recombination protein RecR (NCBI ptt file), at 2,113,551 to 2,114,150
recR
Position (kb)
2109
2110
2111
2112
2113 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2108.982 kb on - strand at 2109.148 kb on + strand at 2109.160 kb on + strand at 2109.185 kb on + strand at 2109.205 kb on + strand at 2109.314 kb on - strand at 2109.529 kb on + strand, within apt at 2109.608 kb on - strand, within apt at 2109.857 kb on + strand at 2112.883 kb on + strand at 2112.903 kb on + strand at 2112.918 kb on + strand at 2112.972 kb on - strand at 2113.040 kb on + strand at 2113.040 kb on + strand at 2113.040 kb on + strand at 2113.040 kb on + strand at 2113.048 kb on - strand at 2113.048 kb on - strand at 2113.048 kb on - strand at 2113.048 kb on - strand at 2113.048 kb on - strand at 2113.175 kb on - strand at 2113.246 kb on - strand, within SO2014 at 2113.576 kb on + strand at 2113.691 kb on + strand, within recR at 2113.691 kb on + strand, within recR at 2113.864 kb on + strand, within recR at 2113.920 kb on + strand, within recR at 2113.922 kb on - strand, within recR
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 2,108,982 - -0.3 2,109,148 + +0.6 2,109,160 + +0.1 2,109,185 + +0.6 2,109,205 + -1.8 2,109,314 - +0.4 2,109,529 + apt SO2012 0.43 +0.6 2,109,608 - apt SO2012 0.58 -0.1 2,109,857 + -0.5 2,112,883 + +0.4 2,112,903 + -1.7 2,112,918 + +0.4 2,112,972 - +0.3 2,113,040 + -0.8 2,113,040 + +0.6 2,113,040 + -0.1 2,113,040 + +0.3 2,113,048 - +1.0 2,113,048 - -0.2 2,113,048 - +0.2 2,113,048 - -0.1 2,113,048 - +0.4 2,113,175 - -3.9 2,113,246 - SO2014 0.11 -2.4 2,113,576 + -4.0 2,113,691 + recR SO2015 0.23 -3.1 2,113,691 + recR SO2015 0.23 -3.4 2,113,864 + recR SO2015 0.52 -3.0 2,113,920 + recR SO2015 0.61 -2.6 2,113,922 - recR SO2015 0.62 -2.1
Or see this region's nucleotide sequence