Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfG and nrfC are separated by 86 nucleotides nrfC and nrfD are separated by 14 nucleotides nrfD and nosL are separated by 119 nucleotides nosL and nosD are separated by 10 nucleotides
SO0482: nrfG - formate-dependent nitrite reductase, nrfG protein (NCBI ptt file), at 506,273 to 506,947
nrfG
SO0483: nrfC - formate-dependent nitrite reductase, nrfC protein (NCBI ptt file), at 507,034 to 507,741
nrfC
SO0484: nrfD - formate-dependent nitrite reductase, nrfD protein (NCBI ptt file), at 507,756 to 508,691
nrfD
SO0485: nosL - nosL protein (NCBI ptt file), at 508,811 to 509,302
nosL
SO0486: nosD - copper ABC transporter, periplasmic copper-binding protein (NCBI ptt file), at 509,313 to 510,587
nosD
Position (kb)
507
508
509 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 506.805 kb on + strand, within nrfG at 506.861 kb on + strand, within nrfG at 506.869 kb on - strand, within nrfG at 506.869 kb on - strand, within nrfG at 507.021 kb on + strand at 507.060 kb on - strand at 507.063 kb on + strand at 507.182 kb on - strand, within nrfC at 507.209 kb on + strand, within nrfC at 507.262 kb on - strand, within nrfC at 507.288 kb on + strand, within nrfC at 507.298 kb on - strand, within nrfC at 507.374 kb on + strand, within nrfC at 507.374 kb on + strand, within nrfC at 507.374 kb on + strand, within nrfC at 507.381 kb on + strand, within nrfC at 507.382 kb on - strand, within nrfC at 507.400 kb on + strand, within nrfC at 507.462 kb on + strand, within nrfC at 507.520 kb on + strand, within nrfC at 507.555 kb on + strand, within nrfC at 507.555 kb on + strand, within nrfC at 507.555 kb on + strand, within nrfC at 507.555 kb on + strand, within nrfC at 507.555 kb on + strand, within nrfC at 507.555 kb on + strand, within nrfC at 507.563 kb on - strand, within nrfC at 507.580 kb on + strand, within nrfC at 507.687 kb on + strand at 507.687 kb on + strand at 507.687 kb on + strand at 507.687 kb on + strand at 507.695 kb on - strand at 507.762 kb on - strand at 507.774 kb on - strand at 507.774 kb on - strand at 507.776 kb on + strand at 507.784 kb on - strand at 507.784 kb on - strand at 507.790 kb on - strand at 507.838 kb on - strand at 507.843 kb on + strand at 507.851 kb on - strand, within nrfD at 507.963 kb on - strand, within nrfD at 508.083 kb on + strand, within nrfD at 508.196 kb on + strand, within nrfD at 508.249 kb on - strand, within nrfD at 508.256 kb on + strand, within nrfD at 508.316 kb on - strand, within nrfD at 508.353 kb on + strand, within nrfD at 508.353 kb on + strand, within nrfD at 508.361 kb on - strand, within nrfD at 508.387 kb on + strand, within nrfD at 508.387 kb on + strand, within nrfD at 508.387 kb on + strand, within nrfD at 508.387 kb on + strand, within nrfD at 508.443 kb on + strand, within nrfD at 508.490 kb on + strand, within nrfD at 508.490 kb on + strand, within nrfD at 508.602 kb on + strand at 508.622 kb on + strand at 508.637 kb on - strand at 508.645 kb on + strand at 508.645 kb on - strand at 508.653 kb on - strand at 508.661 kb on - strand at 508.696 kb on + strand at 508.743 kb on + strand at 508.769 kb on - strand at 508.928 kb on - strand, within nosL at 508.978 kb on + strand, within nosL at 508.978 kb on + strand, within nosL at 508.978 kb on + strand, within nosL at 508.978 kb on + strand, within nosL at 508.978 kb on + strand, within nosL at 508.978 kb on + strand, within nosL at 508.978 kb on + strand, within nosL at 508.978 kb on + strand, within nosL at 508.978 kb on + strand, within nosL at 508.986 kb on - strand, within nosL at 508.986 kb on - strand, within nosL at 508.986 kb on - strand, within nosL at 508.986 kb on - strand, within nosL at 508.986 kb on - strand, within nosL at 508.986 kb on - strand, within nosL at 508.986 kb on - strand, within nosL at 509.024 kb on + strand, within nosL at 509.251 kb on + strand, within nosL at 509.252 kb on + strand, within nosL at 509.252 kb on + strand, within nosL at 509.252 kb on + strand, within nosL at 509.252 kb on + strand, within nosL at 509.260 kb on - strand at 509.260 kb on - strand at 509.260 kb on - strand at 509.267 kb on - strand at 509.274 kb on + strand at 509.382 kb on - strand at 509.420 kb on + strand at 509.589 kb on - strand, within nosD at 509.650 kb on + strand, within nosD at 509.655 kb on - strand, within nosD
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 506,805 + nrfG SO0482 0.79 +0.6 506,861 + nrfG SO0482 0.87 +0.1 506,869 - nrfG SO0482 0.88 +0.1 506,869 - nrfG SO0482 0.88 -2.2 507,021 + +0.8 507,060 - +0.5 507,063 + +0.2 507,182 - nrfC SO0483 0.21 -0.2 507,209 + nrfC SO0483 0.25 +0.8 507,262 - nrfC SO0483 0.32 -0.8 507,288 + nrfC SO0483 0.36 -0.8 507,298 - nrfC SO0483 0.37 -0.8 507,374 + nrfC SO0483 0.48 -0.0 507,374 + nrfC SO0483 0.48 +0.3 507,374 + nrfC SO0483 0.48 +1.7 507,381 + nrfC SO0483 0.49 -0.6 507,382 - nrfC SO0483 0.49 -0.8 507,400 + nrfC SO0483 0.52 -1.8 507,462 + nrfC SO0483 0.60 +0.8 507,520 + nrfC SO0483 0.69 -2.0 507,555 + nrfC SO0483 0.74 +0.8 507,555 + nrfC SO0483 0.74 +0.2 507,555 + nrfC SO0483 0.74 +0.7 507,555 + nrfC SO0483 0.74 -1.5 507,555 + nrfC SO0483 0.74 +0.3 507,555 + nrfC SO0483 0.74 -0.9 507,563 - nrfC SO0483 0.75 -0.7 507,580 + nrfC SO0483 0.77 +0.1 507,687 + -1.1 507,687 + +0.4 507,687 + +0.4 507,687 + +0.6 507,695 - -1.0 507,762 - -0.3 507,774 - -0.7 507,774 - -1.3 507,776 + -0.6 507,784 - +0.8 507,784 - +0.7 507,790 - -0.3 507,838 - -0.4 507,843 + -0.9 507,851 - nrfD SO0484 0.10 -0.9 507,963 - nrfD SO0484 0.22 -1.1 508,083 + nrfD SO0484 0.35 -2.2 508,196 + nrfD SO0484 0.47 +0.1 508,249 - nrfD SO0484 0.53 -1.0 508,256 + nrfD SO0484 0.53 -0.9 508,316 - nrfD SO0484 0.60 -0.3 508,353 + nrfD SO0484 0.64 -0.3 508,353 + nrfD SO0484 0.64 -0.9 508,361 - nrfD SO0484 0.65 -0.8 508,387 + nrfD SO0484 0.67 +0.0 508,387 + nrfD SO0484 0.67 -0.7 508,387 + nrfD SO0484 0.67 -2.8 508,387 + nrfD SO0484 0.67 -0.2 508,443 + nrfD SO0484 0.73 -0.2 508,490 + nrfD SO0484 0.78 -0.7 508,490 + nrfD SO0484 0.78 +0.8 508,602 + +0.0 508,622 + -0.7 508,637 - +1.6 508,645 + +0.4 508,645 - +0.3 508,653 - +0.5 508,661 - +2.8 508,696 + -1.8 508,743 + -0.4 508,769 - -0.9 508,928 - nosL SO0485 0.24 +0.7 508,978 + nosL SO0485 0.34 -0.1 508,978 + nosL SO0485 0.34 -0.4 508,978 + nosL SO0485 0.34 -1.4 508,978 + nosL SO0485 0.34 +0.4 508,978 + nosL SO0485 0.34 -0.4 508,978 + nosL SO0485 0.34 -0.1 508,978 + nosL SO0485 0.34 -0.4 508,978 + nosL SO0485 0.34 -0.4 508,978 + nosL SO0485 0.34 +0.3 508,986 - nosL SO0485 0.36 +0.2 508,986 - nosL SO0485 0.36 +0.3 508,986 - nosL SO0485 0.36 +0.3 508,986 - nosL SO0485 0.36 +0.9 508,986 - nosL SO0485 0.36 -0.8 508,986 - nosL SO0485 0.36 -0.7 508,986 - nosL SO0485 0.36 +0.2 509,024 + nosL SO0485 0.43 -0.7 509,251 + nosL SO0485 0.89 +0.6 509,252 + nosL SO0485 0.90 +0.2 509,252 + nosL SO0485 0.90 +0.5 509,252 + nosL SO0485 0.90 +1.2 509,252 + nosL SO0485 0.90 -0.4 509,260 - +0.5 509,260 - -0.1 509,260 - +0.2 509,267 - -1.2 509,274 + +0.4 509,382 - -0.1 509,420 + +0.2 509,589 - nosD SO0486 0.22 +0.2 509,650 + nosD SO0486 0.26 -0.2 509,655 - nosD SO0486 0.27 +2.8
Or see this region's nucleotide sequence