Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspD and gspE overlap by 4 nucleotides gspE and gspF are separated by 3 nucleotides
SO0166: gspD - general secretion pathway protein D (NCBI ptt file), at 172,301 to 174,415
gspD
SO0167: gspE - general secretion pathway protein E (NCBI ptt file), at 174,412 to 175,977
gspE
SO0168: gspF - general secretion pathway protein F (NCBI ptt file), at 175,981 to 177,204
gspF
Position (kb) 174
175
176 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 173.416 kb on - strand, within gspD at 173.434 kb on - strand, within gspD at 173.500 kb on + strand, within gspD at 173.500 kb on + strand, within gspD at 173.588 kb on + strand, within gspD at 173.606 kb on + strand, within gspD at 173.679 kb on + strand, within gspD at 173.705 kb on - strand, within gspD at 173.781 kb on + strand, within gspD at 173.808 kb on + strand, within gspD at 173.836 kb on + strand, within gspD at 173.871 kb on + strand, within gspD at 174.092 kb on + strand, within gspD at 174.220 kb on + strand at 174.271 kb on + strand at 174.271 kb on + strand at 174.275 kb on - strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.335 kb on + strand at 174.352 kb on + strand at 174.360 kb on - strand at 174.588 kb on + strand, within gspE at 174.646 kb on - strand, within gspE at 174.654 kb on + strand, within gspE at 174.669 kb on - strand, within gspE at 174.702 kb on + strand, within gspE at 174.733 kb on - strand, within gspE at 174.797 kb on + strand, within gspE at 174.807 kb on + strand, within gspE at 174.815 kb on - strand, within gspE at 174.833 kb on + strand, within gspE at 174.843 kb on + strand, within gspE at 174.866 kb on - strand, within gspE at 174.953 kb on - strand, within gspE at 174.976 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 175.007 kb on - strand, within gspE at 175.013 kb on - strand, within gspE at 175.269 kb on + strand, within gspE at 175.285 kb on - strand, within gspE at 175.407 kb on - strand, within gspE at 175.451 kb on - strand, within gspE at 175.484 kb on - strand, within gspE at 175.484 kb on - strand, within gspE at 175.550 kb on - strand, within gspE at 175.570 kb on + strand, within gspE at 175.578 kb on + strand, within gspE at 175.710 kb on + strand, within gspE at 175.817 kb on + strand, within gspE at 175.951 kb on + strand at 175.959 kb on - strand at 175.972 kb on + strand at 175.979 kb on - strand at 175.980 kb on - strand at 175.980 kb on - strand at 176.005 kb on - strand at 176.010 kb on + strand at 176.025 kb on + strand at 176.033 kb on - strand at 176.033 kb on - strand at 176.134 kb on + strand, within gspF at 176.229 kb on - strand, within gspF at 176.247 kb on + strand, within gspF at 176.247 kb on + strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.260 kb on + strand, within gspF at 176.289 kb on - strand, within gspF at 176.327 kb on + strand, within gspF at 176.327 kb on + strand, within gspF at 176.335 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.524 kb on - strand, within gspF at 176.555 kb on - strand, within gspF at 176.597 kb on - strand, within gspF at 176.640 kb on - strand, within gspF at 176.795 kb on - strand, within gspF at 176.834 kb on + strand, within gspF at 176.834 kb on + strand, within gspF at 176.936 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.953 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.966 kb on - strand, within gspF at 176.966 kb on - strand, within gspF
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 173,416 - gspD SO0166 0.53 -1.2 173,434 - gspD SO0166 0.54 -1.6 173,500 + gspD SO0166 0.57 -1.7 173,500 + gspD SO0166 0.57 -1.5 173,588 + gspD SO0166 0.61 -0.3 173,606 + gspD SO0166 0.62 -1.7 173,679 + gspD SO0166 0.65 -2.4 173,705 - gspD SO0166 0.66 -1.4 173,781 + gspD SO0166 0.70 -1.4 173,808 + gspD SO0166 0.71 -0.9 173,836 + gspD SO0166 0.73 +0.3 173,871 + gspD SO0166 0.74 +0.4 174,092 + gspD SO0166 0.85 +0.3 174,220 + -1.1 174,271 + +0.6 174,271 + -0.3 174,275 - -1.0 174,335 + -0.1 174,335 + +0.5 174,335 + -2.8 174,335 + +0.1 174,352 + +0.2 174,360 - -3.1 174,588 + gspE SO0167 0.11 -1.7 174,646 - gspE SO0167 0.15 -2.7 174,654 + gspE SO0167 0.15 +0.9 174,669 - gspE SO0167 0.16 -1.7 174,702 + gspE SO0167 0.19 -1.3 174,733 - gspE SO0167 0.20 -1.0 174,797 + gspE SO0167 0.25 -1.3 174,807 + gspE SO0167 0.25 -1.9 174,815 - gspE SO0167 0.26 -0.7 174,833 + gspE SO0167 0.27 +0.9 174,843 + gspE SO0167 0.28 -0.9 174,866 - gspE SO0167 0.29 -2.4 174,953 - gspE SO0167 0.35 -0.3 174,976 + gspE SO0167 0.36 -1.1 174,999 + gspE SO0167 0.37 -1.1 174,999 + gspE SO0167 0.37 -0.2 174,999 + gspE SO0167 0.37 -0.6 175,007 - gspE SO0167 0.38 -2.3 175,013 - gspE SO0167 0.38 -1.5 175,269 + gspE SO0167 0.55 -1.3 175,285 - gspE SO0167 0.56 -2.0 175,407 - gspE SO0167 0.64 -0.7 175,451 - gspE SO0167 0.66 -1.0 175,484 - gspE SO0167 0.68 -1.6 175,484 - gspE SO0167 0.68 -1.3 175,550 - gspE SO0167 0.73 -0.9 175,570 + gspE SO0167 0.74 -1.3 175,578 + gspE SO0167 0.74 -1.8 175,710 + gspE SO0167 0.83 -3.3 175,817 + gspE SO0167 0.90 +0.9 175,951 + -1.0 175,959 - -0.4 175,972 + -0.6 175,979 - -1.5 175,980 - -1.1 175,980 - -2.6 176,005 - -1.8 176,010 + +0.6 176,025 + -3.3 176,033 - -2.7 176,033 - -1.1 176,134 + gspF SO0168 0.12 +2.5 176,229 - gspF SO0168 0.20 -2.3 176,247 + gspF SO0168 0.22 +0.3 176,247 + gspF SO0168 0.22 +0.9 176,255 - gspF SO0168 0.22 -1.3 176,255 - gspF SO0168 0.22 -1.3 176,255 - gspF SO0168 0.22 -1.0 176,255 - gspF SO0168 0.22 -0.1 176,255 - gspF SO0168 0.22 -0.4 176,260 + gspF SO0168 0.23 -1.7 176,289 - gspF SO0168 0.25 -1.4 176,327 + gspF SO0168 0.28 -1.1 176,327 + gspF SO0168 0.28 -0.9 176,335 - gspF SO0168 0.29 -1.9 176,382 - gspF SO0168 0.33 -0.7 176,382 - gspF SO0168 0.33 +0.6 176,524 - gspF SO0168 0.44 -0.5 176,555 - gspF SO0168 0.47 +0.3 176,597 - gspF SO0168 0.50 -1.7 176,640 - gspF SO0168 0.54 -1.7 176,795 - gspF SO0168 0.67 -2.1 176,834 + gspF SO0168 0.70 -0.1 176,834 + gspF SO0168 0.70 +0.9 176,936 + gspF SO0168 0.78 -1.6 176,941 + gspF SO0168 0.78 -1.9 176,941 + gspF SO0168 0.78 -0.8 176,941 + gspF SO0168 0.78 -1.3 176,953 + gspF SO0168 0.79 -1.3 176,958 + gspF SO0168 0.80 -1.1 176,958 + gspF SO0168 0.80 -2.1 176,966 - gspF SO0168 0.80 -2.4 176,966 - gspF SO0168 0.80 -1.4
Or see this region's nucleotide sequence