Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35170 and PGA1_c35180 are separated by 75 nucleotides PGA1_c35180 and PGA1_c35190 are separated by 78 nucleotides PGA1_c35190 and PGA1_c35200 are separated by 115 nucleotides PGA1_c35200 and PGA1_c35210 overlap by 4 nucleotides
PGA1_c35170: PGA1_c35170 - DNA polymerase III subunit epsilon, at 3,689,382 to 3,690,119
_c35170
PGA1_c35180: PGA1_c35180 - protein-export protein SecB, at 3,690,195 to 3,690,689
_c35180
PGA1_c35190: PGA1_c35190 - putative protein FxsA, at 3,690,768 to 3,691,274
_c35190
PGA1_c35200: PGA1_c35200 - putative transporter protein, at 3,691,390 to 3,692,046
_c35200
PGA1_c35210: PGA1_c35210 - putative membrane-bound lytic murein transglycosylase A, at 3,692,043 to 3,693,098
_c35210
Position (kb)
3690
3691
3692 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3689.989 kb on + strand, within PGA1_c35170 at 3690.007 kb on + strand, within PGA1_c35170 at 3690.093 kb on + strand at 3690.096 kb on + strand at 3690.096 kb on + strand at 3690.096 kb on + strand at 3690.098 kb on + strand at 3690.104 kb on - strand at 3690.161 kb on - strand at 3690.186 kb on + strand at 3690.186 kb on + strand at 3690.186 kb on + strand at 3690.194 kb on - strand at 3690.194 kb on - strand at 3690.194 kb on - strand at 3690.204 kb on + strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.225 kb on - strand at 3690.766 kb on + strand at 3690.771 kb on + strand at 3690.779 kb on + strand at 3690.860 kb on - strand, within PGA1_c35190 at 3690.860 kb on - strand, within PGA1_c35190 at 3690.880 kb on + strand, within PGA1_c35190 at 3690.923 kb on + strand, within PGA1_c35190 at 3690.987 kb on + strand, within PGA1_c35190 at 3690.987 kb on + strand, within PGA1_c35190 at 3690.995 kb on - strand, within PGA1_c35190 at 3691.029 kb on - strand, within PGA1_c35190 at 3691.036 kb on - strand, within PGA1_c35190 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.184 kb on + strand, within PGA1_c35190 at 3691.198 kb on - strand, within PGA1_c35190 at 3691.209 kb on + strand, within PGA1_c35190 at 3691.292 kb on - strand at 3691.292 kb on - strand at 3691.292 kb on - strand at 3691.300 kb on + strand at 3691.311 kb on - strand at 3691.316 kb on - strand at 3691.320 kb on + strand at 3691.330 kb on + strand at 3691.395 kb on - strand at 3691.416 kb on + strand at 3691.435 kb on - strand at 3691.460 kb on - strand, within PGA1_c35200 at 3691.468 kb on - strand, within PGA1_c35200 at 3691.472 kb on + strand, within PGA1_c35200 at 3691.480 kb on - strand, within PGA1_c35200 at 3691.488 kb on - strand, within PGA1_c35200 at 3691.600 kb on - strand, within PGA1_c35200 at 3691.645 kb on - strand, within PGA1_c35200 at 3691.689 kb on + strand, within PGA1_c35200 at 3691.749 kb on - strand, within PGA1_c35200 at 3691.794 kb on - strand, within PGA1_c35200 at 3691.818 kb on - strand, within PGA1_c35200 at 3691.858 kb on - strand, within PGA1_c35200 at 3691.878 kb on + strand, within PGA1_c35200 at 3691.910 kb on - strand, within PGA1_c35200 at 3692.129 kb on + strand at 3692.139 kb on + strand at 3692.139 kb on + strand at 3692.154 kb on + strand, within PGA1_c35210 at 3692.175 kb on - strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210 at 3692.273 kb on + strand, within PGA1_c35210
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 3,689,989 + PGA1_c35170 0.82 +1.1 3,690,007 + PGA1_c35170 0.85 +0.3 3,690,093 + -1.2 3,690,096 + +0.1 3,690,096 + +1.2 3,690,096 + +0.6 3,690,098 + -0.4 3,690,104 - -0.4 3,690,161 - +1.0 3,690,186 + +0.7 3,690,186 + -2.1 3,690,186 + -0.1 3,690,194 - -1.7 3,690,194 - -0.7 3,690,194 - +0.5 3,690,204 + +0.3 3,690,225 - -0.0 3,690,225 - +0.1 3,690,225 - +0.3 3,690,225 - +0.5 3,690,766 + +0.3 3,690,771 + +0.7 3,690,779 + -0.3 3,690,860 - PGA1_c35190 0.18 -1.3 3,690,860 - PGA1_c35190 0.18 -0.0 3,690,880 + PGA1_c35190 0.22 -0.3 3,690,923 + PGA1_c35190 0.31 -0.6 3,690,987 + PGA1_c35190 0.43 -0.0 3,690,987 + PGA1_c35190 0.43 +0.3 3,690,995 - PGA1_c35190 0.45 +0.2 3,691,029 - PGA1_c35190 0.51 -0.8 3,691,036 - PGA1_c35190 0.53 +1.2 3,691,184 + PGA1_c35190 0.82 +0.9 3,691,184 + PGA1_c35190 0.82 -1.8 3,691,184 + PGA1_c35190 0.82 -1.9 3,691,198 - PGA1_c35190 0.85 -0.5 3,691,209 + PGA1_c35190 0.87 +0.6 3,691,292 - -1.4 3,691,292 - -2.2 3,691,292 - +0.1 3,691,300 + +0.1 3,691,311 - -1.6 3,691,316 - -0.0 3,691,320 + -0.4 3,691,330 + +0.0 3,691,395 - -1.5 3,691,416 + -2.5 3,691,435 - -3.3 3,691,460 - PGA1_c35200 0.11 -2.7 3,691,468 - PGA1_c35200 0.12 -2.3 3,691,472 + PGA1_c35200 0.12 -1.8 3,691,480 - PGA1_c35200 0.14 -2.2 3,691,488 - PGA1_c35200 0.15 -0.6 3,691,600 - PGA1_c35200 0.32 -1.2 3,691,645 - PGA1_c35200 0.39 -1.5 3,691,689 + PGA1_c35200 0.46 -2.3 3,691,749 - PGA1_c35200 0.55 +0.3 3,691,794 - PGA1_c35200 0.61 -1.2 3,691,818 - PGA1_c35200 0.65 +0.8 3,691,858 - PGA1_c35200 0.71 -2.5 3,691,878 + PGA1_c35200 0.74 -2.8 3,691,910 - PGA1_c35200 0.79 -0.8 3,692,129 + -0.4 3,692,139 + -1.0 3,692,139 + +0.0 3,692,154 + PGA1_c35210 0.11 -2.5 3,692,175 - PGA1_c35210 0.12 +0.8 3,692,273 + PGA1_c35210 0.22 +0.8 3,692,273 + PGA1_c35210 0.22 -1.1 3,692,273 + PGA1_c35210 0.22 -1.3 3,692,273 + PGA1_c35210 0.22 +0.1 3,692,273 + PGA1_c35210 0.22 -1.0 3,692,273 + PGA1_c35210 0.22 +0.1 3,692,273 + PGA1_c35210 0.22 -0.5 3,692,273 + PGA1_c35210 0.22 -0.2 3,692,273 + PGA1_c35210 0.22 +0.0 3,692,273 + PGA1_c35210 0.22 -0.3 3,692,273 + PGA1_c35210 0.22 -1.5 3,692,273 + PGA1_c35210 0.22 -0.2 3,692,273 + PGA1_c35210 0.22 -0.2 3,692,273 + PGA1_c35210 0.22 -1.2 3,692,273 + PGA1_c35210 0.22 -0.3 3,692,273 + PGA1_c35210 0.22 -1.0 3,692,273 + PGA1_c35210 0.22 +0.3
Or see this region's nucleotide sequence