Experiment: Cisplatin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspB and SO_1299 are separated by 54 nucleotides SO_1299 and SO1298.2 are separated by 14 nucleotides SO1298.2 and SO1298.3 are separated by 2 nucleotides SO1298.3 and SO1298.4 are separated by 9 nucleotides
SO1298: gspB - general secretion pathway protein B (NCBI ptt file), at 1,348,811 to 1,349,917
gspB
SO_1299: SO_1299 - no description, at 1,349,972 to 1,350,145
_1299
SO1298.2: SO1298.2 - hypothetical Na+/H+ antiporter (NCBI ptt file), at 1,350,160 to 1,350,300
SO1298.2
SO1298.3: SO1298.3 - hypothetical Na+/H+ antiporter (NCBI ptt file), at 1,350,303 to 1,350,485
SO1298.3
SO1298.4: SO1298.4 - hypothetical Na+/H+ antiporter (NCBI ptt file), at 1,350,495 to 1,351,079
SO1298.4
Position (kb)
1350
1351 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1349.185 kb on + strand, within gspB at 1349.251 kb on + strand, within gspB at 1349.480 kb on + strand, within gspB at 1349.518 kb on + strand, within gspB at 1349.589 kb on - strand, within gspB at 1349.627 kb on - strand, within gspB at 1349.807 kb on + strand at 1349.873 kb on + strand at 1349.916 kb on + strand at 1349.924 kb on - strand at 1349.983 kb on + strand at 1350.001 kb on + strand, within SO_1299 at 1350.004 kb on - strand, within SO_1299 at 1350.010 kb on - strand, within SO_1299 at 1350.031 kb on + strand, within SO_1299 at 1350.157 kb on + strand at 1350.166 kb on + strand at 1350.187 kb on - strand, within SO1298.2 at 1350.191 kb on + strand, within SO1298.2 at 1350.231 kb on + strand, within SO1298.2 at 1350.238 kb on + strand, within SO1298.2 at 1350.238 kb on + strand, within SO1298.2 at 1350.292 kb on + strand at 1350.459 kb on + strand, within SO1298.3 at 1350.494 kb on + strand at 1350.637 kb on + strand, within SO1298.4 at 1350.652 kb on - strand, within SO1298.4 at 1350.666 kb on + strand, within SO1298.4 at 1350.744 kb on - strand, within SO1298.4 at 1350.866 kb on - strand, within SO1298.4 at 1350.873 kb on - strand, within SO1298.4 at 1350.944 kb on - strand, within SO1298.4 at 1351.070 kb on - strand at 1351.075 kb on + strand at 1351.075 kb on + strand at 1351.075 kb on + strand at 1351.075 kb on + strand at 1351.075 kb on + strand at 1351.083 kb on - strand at 1351.083 kb on - strand at 1351.155 kb on + strand at 1351.247 kb on + strand at 1351.255 kb on - strand at 1351.262 kb on + strand at 1351.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.004 mg/ml remove 1,349,185 + gspB SO1298 0.34 -0.7 1,349,251 + gspB SO1298 0.40 +1.1 1,349,480 + gspB SO1298 0.60 -1.0 1,349,518 + gspB SO1298 0.64 -1.3 1,349,589 - gspB SO1298 0.70 +0.0 1,349,627 - gspB SO1298 0.74 -0.5 1,349,807 + +0.5 1,349,873 + -1.5 1,349,916 + -1.5 1,349,924 - -0.5 1,349,983 + -0.8 1,350,001 + SO_1299 0.17 -1.5 1,350,004 - SO_1299 0.18 +0.7 1,350,010 - SO_1299 0.22 -0.9 1,350,031 + SO_1299 0.34 +0.6 1,350,157 + +1.4 1,350,166 + -0.0 1,350,187 - SO1298.2 0.19 -2.3 1,350,191 + SO1298.2 0.22 -0.2 1,350,231 + SO1298.2 0.50 -1.2 1,350,238 + SO1298.2 0.55 -1.6 1,350,238 + SO1298.2 0.55 +0.0 1,350,292 + -3.1 1,350,459 + SO1298.3 0.85 +1.1 1,350,494 + +0.5 1,350,637 + SO1298.4 0.24 -1.8 1,350,652 - SO1298.4 0.27 +0.8 1,350,666 + SO1298.4 0.29 -0.1 1,350,744 - SO1298.4 0.43 -0.5 1,350,866 - SO1298.4 0.63 +0.5 1,350,873 - SO1298.4 0.65 -1.8 1,350,944 - SO1298.4 0.77 -3.2 1,351,070 - +0.1 1,351,075 + +0.3 1,351,075 + +0.6 1,351,075 + +0.6 1,351,075 + +0.5 1,351,075 + +1.1 1,351,083 - -0.8 1,351,083 - -2.8 1,351,155 + -2.4 1,351,247 + -0.9 1,351,255 - +0.8 1,351,262 + +0.7 1,351,267 - +0.8
Or see this region's nucleotide sequence