Experiment: Cisplatin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3015 and SO3016 are separated by 189 nucleotides SO3016 and SO3017 overlap by 4 nucleotides SO3017 and trpE are separated by 371 nucleotides
SO3015: SO3015 - conserved hypothetical protein (NCBI ptt file), at 3,127,031 to 3,127,819
SO3015
SO3016: SO3016 - Sua5/YciO/YrdC/YwlC family protein (NCBI ptt file), at 3,128,009 to 3,128,629
SO3016
SO3017: SO3017 - TrpH family protein (NCBI ptt file), at 3,128,626 to 3,129,486
SO3017
SO3019: trpE - anthranilate synthase component I (NCBI ptt file), at 3,129,858 to 3,131,582
trpE
Position (kb)
3128
3129
3130 Strain fitness (log2 ratio)
-2
-1
0
1 at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.853 kb on - strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.986 kb on - strand at 3127.998 kb on - strand at 3128.029 kb on + strand at 3128.168 kb on - strand, within SO3016 at 3128.234 kb on - strand, within SO3016 at 3128.244 kb on + strand, within SO3016 at 3128.344 kb on + strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.389 kb on + strand, within SO3016 at 3128.513 kb on + strand, within SO3016 at 3128.598 kb on - strand at 3128.869 kb on - strand, within SO3017 at 3128.891 kb on - strand, within SO3017 at 3128.902 kb on + strand, within SO3017 at 3128.902 kb on + strand, within SO3017 at 3128.902 kb on + strand, within SO3017 at 3128.910 kb on - strand, within SO3017 at 3128.937 kb on + strand, within SO3017 at 3128.984 kb on + strand, within SO3017 at 3129.005 kb on + strand, within SO3017 at 3129.047 kb on - strand, within SO3017 at 3129.073 kb on + strand, within SO3017 at 3129.119 kb on + strand, within SO3017 at 3129.177 kb on + strand, within SO3017 at 3129.197 kb on - strand, within SO3017 at 3129.222 kb on + strand, within SO3017 at 3129.230 kb on - strand, within SO3017 at 3129.267 kb on - strand, within SO3017 at 3129.337 kb on + strand, within SO3017 at 3129.488 kb on + strand at 3129.686 kb on - strand at 3129.714 kb on + strand at 3129.740 kb on + strand at 3129.741 kb on - strand at 3129.748 kb on - strand at 3129.748 kb on - strand at 3129.913 kb on + strand at 3130.075 kb on - strand, within trpE at 3130.113 kb on + strand, within trpE at 3130.215 kb on + strand, within trpE at 3130.215 kb on + strand, within trpE at 3130.223 kb on - strand, within trpE at 3130.223 kb on - strand, within trpE at 3130.320 kb on + strand, within trpE at 3130.360 kb on - strand, within trpE
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.004 mg/ml remove 3,127,845 + -1.3 3,127,845 + +1.6 3,127,845 + +0.4 3,127,845 + +1.6 3,127,853 - -0.4 3,127,978 + +1.1 3,127,978 + -1.4 3,127,978 + +0.6 3,127,978 + -1.2 3,127,986 - -1.1 3,127,998 - -1.1 3,128,029 + -1.5 3,128,168 - SO3016 0.26 +1.0 3,128,234 - SO3016 0.36 +0.4 3,128,244 + SO3016 0.38 +1.6 3,128,344 + SO3016 0.54 -1.5 3,128,352 - SO3016 0.55 +0.1 3,128,352 - SO3016 0.55 -0.2 3,128,352 - SO3016 0.55 -2.7 3,128,389 + SO3016 0.61 -0.5 3,128,513 + SO3016 0.81 +0.1 3,128,598 - -1.3 3,128,869 - SO3017 0.28 -1.1 3,128,891 - SO3017 0.31 -2.3 3,128,902 + SO3017 0.32 -1.1 3,128,902 + SO3017 0.32 +0.1 3,128,902 + SO3017 0.32 -0.3 3,128,910 - SO3017 0.33 -1.0 3,128,937 + SO3017 0.36 +0.0 3,128,984 + SO3017 0.42 +0.4 3,129,005 + SO3017 0.44 -2.4 3,129,047 - SO3017 0.49 +0.5 3,129,073 + SO3017 0.52 -1.2 3,129,119 + SO3017 0.57 -0.8 3,129,177 + SO3017 0.64 -0.9 3,129,197 - SO3017 0.66 -2.0 3,129,222 + SO3017 0.69 -1.1 3,129,230 - SO3017 0.70 -0.8 3,129,267 - SO3017 0.74 +0.3 3,129,337 + SO3017 0.83 -0.2 3,129,488 + +0.6 3,129,686 - -1.0 3,129,714 + +1.1 3,129,740 + -0.3 3,129,741 - +0.7 3,129,748 - -2.2 3,129,748 - +1.0 3,129,913 + -0.1 3,130,075 - trpE SO3019 0.13 +0.7 3,130,113 + trpE SO3019 0.15 -0.5 3,130,215 + trpE SO3019 0.21 -0.8 3,130,215 + trpE SO3019 0.21 -1.3 3,130,223 - trpE SO3019 0.21 -2.7 3,130,223 - trpE SO3019 0.21 +1.0 3,130,320 + trpE SO3019 0.27 -0.4 3,130,360 - trpE SO3019 0.29 -0.9
Or see this region's nucleotide sequence