Experiment: Cisplatin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2704 and topA are separated by 143 nucleotides topA and astB are separated by 331 nucleotides
SO2704: SO2704 - prophage MuSo2, tail fiber protein, putative (NCBI ptt file), at 2,821,109 to 2,822,836
SO2704
SO2705: topA - DNA topoisomerase I (NCBI ptt file), at 2,822,980 to 2,825,616
topA
SO2706: astB - Succinylarginine dihydrolase (EC 3.5.3.23) (from data) , at 2,825,948 to 2,827,282
astB
Position (kb)
2822
2823
2824
2825
2826 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2821.993 kb on + strand, within SO2704 at 2822.281 kb on + strand, within SO2704 at 2822.289 kb on - strand, within SO2704 at 2822.342 kb on + strand, within SO2704 at 2822.343 kb on + strand, within SO2704 at 2822.438 kb on + strand, within SO2704 at 2822.446 kb on - strand, within SO2704 at 2822.446 kb on - strand, within SO2704 at 2822.446 kb on - strand, within SO2704 at 2822.446 kb on - strand, within SO2704 at 2822.446 kb on - strand, within SO2704 at 2822.449 kb on + strand, within SO2704 at 2822.498 kb on + strand, within SO2704 at 2822.498 kb on + strand, within SO2704 at 2822.498 kb on + strand, within SO2704 at 2822.501 kb on + strand, within SO2704 at 2822.506 kb on - strand, within SO2704 at 2822.506 kb on - strand, within SO2704 at 2822.506 kb on - strand, within SO2704 at 2822.541 kb on + strand, within SO2704 at 2822.541 kb on + strand, within SO2704 at 2822.541 kb on + strand, within SO2704 at 2822.541 kb on + strand, within SO2704 at 2822.541 kb on + strand, within SO2704 at 2822.549 kb on - strand, within SO2704 at 2822.549 kb on - strand, within SO2704 at 2822.549 kb on - strand, within SO2704 at 2822.549 kb on - strand, within SO2704 at 2822.549 kb on - strand, within SO2704 at 2822.559 kb on - strand, within SO2704 at 2822.559 kb on - strand, within SO2704 at 2822.576 kb on - strand, within SO2704 at 2822.646 kb on + strand, within SO2704 at 2822.726 kb on - strand at 2822.736 kb on + strand at 2822.744 kb on - strand at 2822.789 kb on + strand at 2822.789 kb on + strand at 2822.797 kb on - strand at 2822.797 kb on - strand at 2822.852 kb on - strand at 2822.913 kb on - strand at 2822.930 kb on + strand at 2822.933 kb on + strand at 2825.891 kb on - strand at 2826.135 kb on + strand, within astB at 2826.238 kb on - strand, within astB at 2826.243 kb on - strand, within astB at 2826.378 kb on - strand, within astB at 2826.396 kb on - strand, within astB at 2826.420 kb on + strand, within astB at 2826.508 kb on - strand, within astB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.004 mg/ml remove 2,821,993 + SO2704 0.51 +3.2 2,822,281 + SO2704 0.68 +4.0 2,822,289 - SO2704 0.68 +3.5 2,822,342 + SO2704 0.71 +0.6 2,822,343 + SO2704 0.71 +3.2 2,822,438 + SO2704 0.77 +2.7 2,822,446 - SO2704 0.77 +4.3 2,822,446 - SO2704 0.77 -0.5 2,822,446 - SO2704 0.77 +2.1 2,822,446 - SO2704 0.77 +1.5 2,822,446 - SO2704 0.77 +2.3 2,822,449 + SO2704 0.78 +3.4 2,822,498 + SO2704 0.80 +3.6 2,822,498 + SO2704 0.80 +1.9 2,822,498 + SO2704 0.80 +3.2 2,822,501 + SO2704 0.81 +2.9 2,822,506 - SO2704 0.81 +2.5 2,822,506 - SO2704 0.81 +3.1 2,822,506 - SO2704 0.81 +2.8 2,822,541 + SO2704 0.83 +3.6 2,822,541 + SO2704 0.83 +3.4 2,822,541 + SO2704 0.83 +3.2 2,822,541 + SO2704 0.83 +1.6 2,822,541 + SO2704 0.83 +3.3 2,822,549 - SO2704 0.83 +2.9 2,822,549 - SO2704 0.83 +3.4 2,822,549 - SO2704 0.83 +2.3 2,822,549 - SO2704 0.83 +1.9 2,822,549 - SO2704 0.83 +2.8 2,822,559 - SO2704 0.84 +3.8 2,822,559 - SO2704 0.84 +3.8 2,822,576 - SO2704 0.85 +3.3 2,822,646 + SO2704 0.89 +1.1 2,822,726 - +2.8 2,822,736 + +3.3 2,822,744 - +3.4 2,822,789 + +1.0 2,822,789 + +3.7 2,822,797 - +3.9 2,822,797 - +1.4 2,822,852 - +3.2 2,822,913 - -0.0 2,822,930 + -0.6 2,822,933 + -0.3 2,825,891 - -1.8 2,826,135 + astB SO2706 0.14 -1.5 2,826,238 - astB SO2706 0.22 -2.4 2,826,243 - astB SO2706 0.22 -1.0 2,826,378 - astB SO2706 0.32 +0.9 2,826,396 - astB SO2706 0.34 -2.3 2,826,420 + astB SO2706 0.35 -0.6 2,826,508 - astB SO2706 0.42 -1.5
Or see this region's nucleotide sequence