Experiment: Cisplatin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2308 and crcB overlap by 8 nucleotides crcB and serS are separated by 18 nucleotides serS and SO2311 are separated by 18 nucleotides SO2311 and SO2312 are separated by 78 nucleotides
SO2308: SO2308 - ATPase, AAA family (NCBI ptt file), at 2,420,521 to 2,421,858
SO2308
SO2309: crcB - crcB protein (NCBI ptt file), at 2,421,851 to 2,422,225
crcB
SO2310: serS - seryl-tRNA synthetase (NCBI ptt file), at 2,422,244 to 2,423,530
serS
SO2311: SO2311 - hypothetical protein (NCBI ptt file), at 2,423,549 to 2,423,671
SO2311
SO2312: SO2312 - serine protease inhibitor, ecotin (NCBI ptt file), at 2,423,750 to 2,424,301
SO2312
Position (kb)
2422
2423
2424 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2421.549 kb on + strand, within SO2308 at 2421.612 kb on - strand, within SO2308 at 2421.689 kb on + strand, within SO2308 at 2421.699 kb on + strand, within SO2308 at 2421.826 kb on + strand at 2421.867 kb on - strand at 2421.874 kb on - strand at 2421.877 kb on + strand at 2421.995 kb on + strand, within crcB at 2423.542 kb on - strand at 2423.542 kb on - strand at 2423.547 kb on - strand at 2423.559 kb on - strand at 2423.579 kb on + strand, within SO2311 at 2423.632 kb on + strand, within SO2311 at 2423.635 kb on - strand, within SO2311 at 2423.640 kb on - strand, within SO2311 at 2423.640 kb on - strand, within SO2311 at 2423.649 kb on - strand, within SO2311 at 2423.786 kb on - strand at 2423.803 kb on - strand at 2423.808 kb on - strand, within SO2312 at 2423.854 kb on + strand, within SO2312 at 2423.854 kb on + strand, within SO2312 at 2423.907 kb on - strand, within SO2312 at 2423.907 kb on - strand, within SO2312 at 2423.969 kb on + strand, within SO2312 at 2423.972 kb on - strand, within SO2312 at 2423.977 kb on - strand, within SO2312 at 2424.032 kb on + strand, within SO2312 at 2424.039 kb on - strand, within SO2312 at 2424.040 kb on - strand, within SO2312 at 2424.062 kb on - strand, within SO2312 at 2424.086 kb on - strand, within SO2312 at 2424.103 kb on + strand, within SO2312 at 2424.219 kb on - strand, within SO2312 at 2424.250 kb on + strand at 2424.253 kb on - strand at 2424.257 kb on + strand at 2424.265 kb on - strand at 2424.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.004 mg/ml remove 2,421,549 + SO2308 0.77 -0.9 2,421,612 - SO2308 0.82 -2.6 2,421,689 + SO2308 0.87 +1.1 2,421,699 + SO2308 0.88 -3.0 2,421,826 + +0.5 2,421,867 - -0.7 2,421,874 - -0.4 2,421,877 + -2.5 2,421,995 + crcB SO2309 0.38 +1.0 2,423,542 - +0.5 2,423,542 - -0.2 2,423,547 - +0.2 2,423,559 - -2.4 2,423,579 + SO2311 0.24 +0.7 2,423,632 + SO2311 0.67 -1.2 2,423,635 - SO2311 0.70 -0.3 2,423,640 - SO2311 0.74 -1.9 2,423,640 - SO2311 0.74 -0.5 2,423,649 - SO2311 0.81 +1.4 2,423,786 - -1.2 2,423,803 - +1.2 2,423,808 - SO2312 0.11 +1.0 2,423,854 + SO2312 0.19 -1.0 2,423,854 + SO2312 0.19 +0.2 2,423,907 - SO2312 0.28 +0.7 2,423,907 - SO2312 0.28 +0.8 2,423,969 + SO2312 0.40 -0.1 2,423,972 - SO2312 0.40 -0.7 2,423,977 - SO2312 0.41 +0.5 2,424,032 + SO2312 0.51 +0.5 2,424,039 - SO2312 0.52 -0.8 2,424,040 - SO2312 0.53 +0.5 2,424,062 - SO2312 0.57 +0.4 2,424,086 - SO2312 0.61 +1.1 2,424,103 + SO2312 0.64 +0.4 2,424,219 - SO2312 0.85 +0.0 2,424,250 + -1.8 2,424,253 - -0.7 2,424,257 + -2.8 2,424,265 - -0.2 2,424,387 - -1.0
Or see this region's nucleotide sequence