Experiment: Cisplatin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1860 and uvrC are separated by 106 nucleotides uvrC and pgsA are separated by 47 nucleotides pgsA and tRNA-Gly-5 are separated by 143 nucleotides tRNA-Gly-5 and tRNA-Cys-1 are separated by 21 nucleotides tRNA-Cys-1 and tRNA-Leu-2 are separated by 48 nucleotides
SO1860: SO1860 - DNA-binding response regulator, LuxR family (NCBI ptt file), at 1,951,805 to 1,952,449
SO1860
SO1861: uvrC - excinuclease ABC, C subunit (NCBI ptt file), at 1,952,556 to 1,954,385
uvrC
SO1862: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (NCBI ptt file), at 1,954,433 to 1,954,981
pgsA
SO_t033: tRNA-Gly-5 - tRNA-Gly (RefSeq), at 1,955,125 to 1,955,200
tRNA-Gly-5
SO_t034: tRNA-Cys-1 - tRNA-Cys (RefSeq), at 1,955,222 to 1,955,295
tRNA-Cys-1
SO_t035: tRNA-Leu-2 - tRNA-Leu (RefSeq), at 1,955,344 to 1,955,430
tRNA-Leu-2
Position (kb)
1952
1953
1954
1955 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1951.580 kb on + strand at 1951.596 kb on + strand at 1951.719 kb on + strand at 1951.761 kb on - strand at 1951.780 kb on - strand at 1951.780 kb on - strand at 1951.780 kb on - strand at 1951.780 kb on - strand at 1951.780 kb on - strand at 1951.782 kb on + strand at 1951.787 kb on + strand at 1951.787 kb on + strand at 1951.787 kb on + strand at 1951.787 kb on + strand at 1951.787 kb on + strand at 1951.787 kb on + strand at 1951.787 kb on + strand at 1951.795 kb on - strand at 1951.830 kb on + strand at 1951.840 kb on + strand at 1951.840 kb on + strand at 1951.840 kb on + strand at 1951.845 kb on + strand at 1951.845 kb on + strand at 1951.853 kb on - strand at 1951.853 kb on - strand at 1951.853 kb on - strand at 1951.853 kb on - strand at 1951.929 kb on + strand, within SO1860 at 1951.937 kb on - strand, within SO1860 at 1951.942 kb on - strand, within SO1860 at 1952.057 kb on + strand, within SO1860 at 1952.135 kb on - strand, within SO1860 at 1952.168 kb on - strand, within SO1860 at 1952.299 kb on + strand, within SO1860 at 1952.300 kb on - strand, within SO1860 at 1952.320 kb on + strand, within SO1860 at 1952.320 kb on + strand, within SO1860 at 1952.328 kb on - strand, within SO1860 at 1952.347 kb on + strand, within SO1860 at 1952.385 kb on + strand at 1952.391 kb on + strand at 1952.391 kb on + strand at 1952.399 kb on - strand at 1952.489 kb on + strand at 1952.565 kb on + strand at 1952.630 kb on + strand at 1952.767 kb on + strand, within uvrC at 1952.816 kb on + strand, within uvrC at 1952.824 kb on - strand, within uvrC at 1952.898 kb on - strand, within uvrC at 1952.899 kb on + strand, within uvrC at 1952.947 kb on - strand, within uvrC at 1952.953 kb on - strand, within uvrC at 1952.977 kb on - strand, within uvrC at 1953.008 kb on + strand, within uvrC at 1953.323 kb on + strand, within uvrC at 1953.326 kb on - strand, within uvrC at 1953.331 kb on - strand, within uvrC at 1953.354 kb on - strand, within uvrC at 1953.435 kb on + strand, within uvrC at 1953.435 kb on + strand, within uvrC at 1953.450 kb on - strand, within uvrC at 1953.543 kb on - strand, within uvrC at 1953.745 kb on + strand, within uvrC at 1953.778 kb on + strand, within uvrC at 1953.795 kb on + strand, within uvrC at 1953.864 kb on - strand, within uvrC at 1954.055 kb on + strand, within uvrC at 1954.063 kb on - strand, within uvrC at 1954.129 kb on + strand, within uvrC
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.004 mg/ml remove 1,951,580 + -2.8 1,951,596 + -2.2 1,951,719 + -0.2 1,951,761 - -2.4 1,951,780 - -4.3 1,951,780 - -3.8 1,951,780 - -1.8 1,951,780 - -2.1 1,951,780 - -2.5 1,951,782 + -0.9 1,951,787 + -2.2 1,951,787 + -2.6 1,951,787 + -1.5 1,951,787 + -0.2 1,951,787 + +0.0 1,951,787 + +0.4 1,951,787 + -2.8 1,951,795 - -1.7 1,951,830 + -3.2 1,951,840 + -0.6 1,951,840 + -1.5 1,951,840 + -0.8 1,951,845 + -1.8 1,951,845 + -3.0 1,951,853 - -3.7 1,951,853 - -2.5 1,951,853 - -4.2 1,951,853 - -4.0 1,951,929 + SO1860 0.19 -4.9 1,951,937 - SO1860 0.20 -4.1 1,951,942 - SO1860 0.21 -3.9 1,952,057 + SO1860 0.39 -1.9 1,952,135 - SO1860 0.51 -1.4 1,952,168 - SO1860 0.56 -3.4 1,952,299 + SO1860 0.77 -1.8 1,952,300 - SO1860 0.77 -1.3 1,952,320 + SO1860 0.80 -2.7 1,952,320 + SO1860 0.80 +0.0 1,952,328 - SO1860 0.81 -1.6 1,952,347 + SO1860 0.84 -3.1 1,952,385 + -1.7 1,952,391 + -1.4 1,952,391 + -2.3 1,952,399 - -3.0 1,952,489 + +0.1 1,952,565 + -1.5 1,952,630 + -3.3 1,952,767 + uvrC SO1861 0.12 -1.1 1,952,816 + uvrC SO1861 0.14 -1.7 1,952,824 - uvrC SO1861 0.15 -3.4 1,952,898 - uvrC SO1861 0.19 -3.6 1,952,899 + uvrC SO1861 0.19 -2.3 1,952,947 - uvrC SO1861 0.21 -2.0 1,952,953 - uvrC SO1861 0.22 -2.7 1,952,977 - uvrC SO1861 0.23 -1.9 1,953,008 + uvrC SO1861 0.25 -2.0 1,953,323 + uvrC SO1861 0.42 -3.1 1,953,326 - uvrC SO1861 0.42 -2.3 1,953,331 - uvrC SO1861 0.42 -3.3 1,953,354 - uvrC SO1861 0.44 -1.1 1,953,435 + uvrC SO1861 0.48 -2.8 1,953,435 + uvrC SO1861 0.48 -4.9 1,953,450 - uvrC SO1861 0.49 +0.4 1,953,543 - uvrC SO1861 0.54 -2.4 1,953,745 + uvrC SO1861 0.65 -2.3 1,953,778 + uvrC SO1861 0.67 -2.3 1,953,795 + uvrC SO1861 0.68 +0.2 1,953,864 - uvrC SO1861 0.71 -2.3 1,954,055 + uvrC SO1861 0.82 -4.3 1,954,063 - uvrC SO1861 0.82 -2.4 1,954,129 + uvrC SO1861 0.86 -0.8
Or see this region's nucleotide sequence