Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01960 and PGA1_c01970 are separated by 4 nucleotides PGA1_c01970 and PGA1_c01980 are separated by 229 nucleotides PGA1_c01980 and PGA1_c01990 are separated by 143 nucleotides
PGA1_c01960: PGA1_c01960 - putative hydrolase, at 178,978 to 179,646
_c01960
PGA1_c01970: PGA1_c01970 - Chaperone required for the assembly of the mitochondrial F1-ATPase, at 179,651 to 180,358
_c01970
PGA1_c01980: PGA1_c01980 - glutamate/glutamine/aspartate/asparagine-binding protein BztA, at 180,588 to 181,604
_c01980
PGA1_c01990: PGA1_c01990 - glutamate/glutamine/aspartate/asparagine transport system permease protein BztB, at 181,748 to 182,980
_c01990
Position (kb) 180
181
182 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 179.631 kb on + strand at 179.653 kb on - strand at 180.345 kb on - strand at 180.422 kb on + strand at 180.430 kb on - strand at 180.459 kb on - strand at 180.476 kb on + strand at 180.528 kb on - strand at 180.531 kb on + strand at 180.536 kb on + strand at 180.571 kb on + strand at 180.617 kb on + strand at 180.617 kb on + strand at 180.658 kb on + strand at 180.708 kb on + strand, within PGA1_c01980 at 180.711 kb on - strand, within PGA1_c01980 at 180.711 kb on - strand, within PGA1_c01980 at 180.711 kb on - strand, within PGA1_c01980 at 180.718 kb on + strand, within PGA1_c01980 at 180.718 kb on + strand, within PGA1_c01980 at 180.718 kb on + strand, within PGA1_c01980 at 180.726 kb on - strand, within PGA1_c01980 at 180.728 kb on + strand, within PGA1_c01980 at 180.754 kb on + strand, within PGA1_c01980 at 180.792 kb on + strand, within PGA1_c01980 at 180.918 kb on + strand, within PGA1_c01980 at 180.957 kb on + strand, within PGA1_c01980 at 180.965 kb on - strand, within PGA1_c01980 at 181.013 kb on - strand, within PGA1_c01980 at 181.090 kb on + strand, within PGA1_c01980 at 181.096 kb on + strand, within PGA1_c01980 at 181.161 kb on - strand, within PGA1_c01980 at 181.172 kb on + strand, within PGA1_c01980 at 181.312 kb on - strand, within PGA1_c01980 at 181.325 kb on + strand, within PGA1_c01980 at 181.580 kb on + strand at 181.627 kb on + strand at 181.635 kb on - strand at 181.655 kb on + strand at 181.688 kb on - strand at 181.714 kb on + strand at 181.721 kb on + strand at 181.757 kb on + strand at 181.779 kb on + strand at 181.850 kb on + strand at 181.855 kb on + strand at 181.866 kb on + strand at 181.892 kb on - strand, within PGA1_c01990 at 181.910 kb on + strand, within PGA1_c01990 at 181.918 kb on - strand, within PGA1_c01990 at 181.918 kb on - strand, within PGA1_c01990 at 182.021 kb on + strand, within PGA1_c01990 at 182.084 kb on - strand, within PGA1_c01990 at 182.095 kb on + strand, within PGA1_c01990 at 182.281 kb on - strand, within PGA1_c01990 at 182.339 kb on - strand, within PGA1_c01990 at 182.371 kb on + strand, within PGA1_c01990 at 182.371 kb on + strand, within PGA1_c01990 at 182.371 kb on + strand, within PGA1_c01990 at 182.379 kb on - strand, within PGA1_c01990 at 182.384 kb on - strand, within PGA1_c01990 at 182.407 kb on - strand, within PGA1_c01990 at 182.483 kb on - strand, within PGA1_c01990 at 182.515 kb on + strand, within PGA1_c01990 at 182.523 kb on - strand, within PGA1_c01990 at 182.523 kb on - strand, within PGA1_c01990 at 182.525 kb on - strand, within PGA1_c01990 at 182.556 kb on - strand, within PGA1_c01990 at 182.592 kb on + strand, within PGA1_c01990
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 179,631 + -2.7 179,653 - -1.2 180,345 - -0.6 180,422 + +0.5 180,430 - -2.3 180,459 - -0.4 180,476 + -0.8 180,528 - -2.1 180,531 + +0.4 180,536 + -2.5 180,571 + -3.1 180,617 + -2.7 180,617 + -2.5 180,658 + -3.8 180,708 + PGA1_c01980 0.12 -2.9 180,711 - PGA1_c01980 0.12 -1.0 180,711 - PGA1_c01980 0.12 -1.6 180,711 - PGA1_c01980 0.12 -3.2 180,718 + PGA1_c01980 0.13 -0.7 180,718 + PGA1_c01980 0.13 -1.0 180,718 + PGA1_c01980 0.13 -2.7 180,726 - PGA1_c01980 0.14 -1.1 180,728 + PGA1_c01980 0.14 -1.4 180,754 + PGA1_c01980 0.16 -2.8 180,792 + PGA1_c01980 0.20 -1.4 180,918 + PGA1_c01980 0.32 -1.6 180,957 + PGA1_c01980 0.36 -2.0 180,965 - PGA1_c01980 0.37 -2.7 181,013 - PGA1_c01980 0.42 -0.8 181,090 + PGA1_c01980 0.49 -1.4 181,096 + PGA1_c01980 0.50 -2.4 181,161 - PGA1_c01980 0.56 -1.7 181,172 + PGA1_c01980 0.57 -1.2 181,312 - PGA1_c01980 0.71 -1.2 181,325 + PGA1_c01980 0.72 -2.4 181,580 + -3.0 181,627 + -0.4 181,635 - +0.6 181,655 + -0.5 181,688 - -2.3 181,714 + -1.1 181,721 + -2.6 181,757 + -2.6 181,779 + -0.6 181,850 + -3.5 181,855 + -0.2 181,866 + -0.1 181,892 - PGA1_c01990 0.12 -1.7 181,910 + PGA1_c01990 0.13 -2.2 181,918 - PGA1_c01990 0.14 -1.6 181,918 - PGA1_c01990 0.14 -2.6 182,021 + PGA1_c01990 0.22 -0.7 182,084 - PGA1_c01990 0.27 -1.0 182,095 + PGA1_c01990 0.28 -1.8 182,281 - PGA1_c01990 0.43 -2.0 182,339 - PGA1_c01990 0.48 -1.0 182,371 + PGA1_c01990 0.51 -0.6 182,371 + PGA1_c01990 0.51 -1.9 182,371 + PGA1_c01990 0.51 -1.7 182,379 - PGA1_c01990 0.51 -0.9 182,384 - PGA1_c01990 0.52 -0.8 182,407 - PGA1_c01990 0.53 -1.5 182,483 - PGA1_c01990 0.60 -2.1 182,515 + PGA1_c01990 0.62 -1.5 182,523 - PGA1_c01990 0.63 -1.2 182,523 - PGA1_c01990 0.63 -2.3 182,525 - PGA1_c01990 0.63 +0.3 182,556 - PGA1_c01990 0.66 -1.7 182,592 + PGA1_c01990 0.68 -2.4
Or see this region's nucleotide sequence