Experiment: Cisplatin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dfp and dut are separated by 0 nucleotides dut and SO4251 are separated by 31 nucleotides SO4251 and SO4252 are separated by 134 nucleotides
SO4249: dfp - DNA/pantothenate metabolism flavoprotein (NCBI ptt file), at 4,426,076 to 4,427,302
dfp
SO4250: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (NCBI ptt file), at 4,427,303 to 4,427,761
dut
SO4251: SO4251 - transcriptional regulator, TetR family (NCBI ptt file), at 4,427,793 to 4,428,386
SO4251
SO4252: SO4252 - prolyl oligopeptidase family protein (NCBI ptt file), at 4,428,521 to 4,430,509
SO4252
Position (kb)
4427
4428 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4427.375 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.519 kb on + strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.546 kb on - strand, within dut at 4427.622 kb on + strand, within dut at 4427.665 kb on - strand, within dut at 4427.791 kb on - strand at 4427.795 kb on + strand at 4427.840 kb on - strand at 4427.840 kb on - strand at 4427.863 kb on + strand, within SO4251 at 4427.908 kb on + strand, within SO4251 at 4427.993 kb on + strand, within SO4251 at 4428.036 kb on + strand, within SO4251 at 4428.057 kb on + strand, within SO4251 at 4428.232 kb on - strand, within SO4251 at 4428.252 kb on + strand, within SO4251 at 4428.338 kb on - strand at 4428.655 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.002 mg/ml remove 4,427,375 + dut SO4250 0.16 -1.1 4,427,514 + dut SO4250 0.46 -0.9 4,427,514 + dut SO4250 0.46 -0.4 4,427,514 + dut SO4250 0.46 -0.1 4,427,514 + dut SO4250 0.46 -1.8 4,427,514 + dut SO4250 0.46 -1.3 4,427,519 + dut SO4250 0.47 -0.4 4,427,522 - dut SO4250 0.48 -2.2 4,427,522 - dut SO4250 0.48 +0.3 4,427,522 - dut SO4250 0.48 -1.6 4,427,546 - dut SO4250 0.53 -2.3 4,427,622 + dut SO4250 0.69 -0.1 4,427,665 - dut SO4250 0.79 -1.2 4,427,791 - -3.1 4,427,795 + -1.5 4,427,840 - -0.8 4,427,840 - -0.5 4,427,863 + SO4251 0.12 -1.8 4,427,908 + SO4251 0.19 -1.4 4,427,993 + SO4251 0.34 -1.4 4,428,036 + SO4251 0.41 -0.6 4,428,057 + SO4251 0.44 -0.4 4,428,232 - SO4251 0.74 -2.0 4,428,252 + SO4251 0.77 -1.5 4,428,338 - -3.4 4,428,655 - -0.6
Or see this region's nucleotide sequence