Strain Fitness in Shewanella oneidensis MR-1 around SO4249

Experiment: Cisplatin 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpmB and radC are separated by 121 nucleotidesradC and dfp are separated by 122 nucleotidesdfp and dut are separated by 0 nucleotidesdut and SO4251 are separated by 31 nucleotides SO4247: rpmB - ribosomal protein L28 (NCBI ptt file), at 4,424,918 to 4,425,154 rpmB SO4248: radC - DNA repair protein RadC (NCBI ptt file), at 4,425,276 to 4,425,953 radC SO4249: dfp - DNA/pantothenate metabolism flavoprotein (NCBI ptt file), at 4,426,076 to 4,427,302 dfp SO4250: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (NCBI ptt file), at 4,427,303 to 4,427,761 dut SO4251: SO4251 - transcriptional regulator, TetR family (NCBI ptt file), at 4,427,793 to 4,428,386 SO4251 Position (kb) 4426 4427 4428Strain fitness (log2 ratio) -3 -2 -1 0 1at 4425.358 kb on - strand, within radCat 4425.443 kb on + strand, within radCat 4425.478 kb on - strand, within radCat 4425.494 kb on + strand, within radCat 4425.547 kb on + strand, within radCat 4425.584 kb on + strand, within radCat 4425.584 kb on + strand, within radCat 4425.584 kb on + strand, within radCat 4425.592 kb on - strand, within radCat 4425.592 kb on - strand, within radCat 4425.592 kb on - strand, within radCat 4425.658 kb on + strand, within radCat 4425.703 kb on + strand, within radCat 4425.711 kb on - strand, within radCat 4425.818 kb on + strand, within radCat 4425.818 kb on + strand, within radCat 4425.826 kb on - strand, within radCat 4425.826 kb on - strand, within radCat 4425.850 kb on - strand, within radCat 4425.907 kb on + strandat 4425.920 kb on + strandat 4425.928 kb on - strandat 4425.934 kb on + strandat 4427.375 kb on + strand, within dutat 4427.514 kb on + strand, within dutat 4427.514 kb on + strand, within dutat 4427.514 kb on + strand, within dutat 4427.514 kb on + strand, within dutat 4427.514 kb on + strand, within dutat 4427.519 kb on + strand, within dutat 4427.522 kb on - strand, within dutat 4427.522 kb on - strand, within dutat 4427.522 kb on - strand, within dutat 4427.546 kb on - strand, within dutat 4427.622 kb on + strand, within dutat 4427.665 kb on - strand, within dutat 4427.791 kb on - strandat 4427.795 kb on + strandat 4427.840 kb on - strandat 4427.840 kb on - strandat 4427.863 kb on + strand, within SO4251at 4427.908 kb on + strand, within SO4251at 4427.993 kb on + strand, within SO4251at 4428.036 kb on + strand, within SO4251at 4428.057 kb on + strand, within SO4251at 4428.232 kb on - strand, within SO4251at 4428.252 kb on + strand, within SO4251

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.002 mg/ml
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4,425,358 - radC SO4248 0.12 -1.6
4,425,443 + radC SO4248 0.25 +0.2
4,425,478 - radC SO4248 0.30 -0.3
4,425,494 + radC SO4248 0.32 -1.5
4,425,547 + radC SO4248 0.40 -1.4
4,425,584 + radC SO4248 0.45 -0.6
4,425,584 + radC SO4248 0.45 -1.3
4,425,584 + radC SO4248 0.45 -0.4
4,425,592 - radC SO4248 0.47 -2.3
4,425,592 - radC SO4248 0.47 -0.2
4,425,592 - radC SO4248 0.47 -0.4
4,425,658 + radC SO4248 0.56 +1.3
4,425,703 + radC SO4248 0.63 -1.1
4,425,711 - radC SO4248 0.64 +0.7
4,425,818 + radC SO4248 0.80 -0.1
4,425,818 + radC SO4248 0.80 -0.2
4,425,826 - radC SO4248 0.81 -0.6
4,425,826 - radC SO4248 0.81 +0.2
4,425,850 - radC SO4248 0.85 +0.8
4,425,907 + -1.2
4,425,920 + +0.0
4,425,928 - -0.2
4,425,934 + +0.1
4,427,375 + dut SO4250 0.16 -1.1
4,427,514 + dut SO4250 0.46 -0.9
4,427,514 + dut SO4250 0.46 -0.4
4,427,514 + dut SO4250 0.46 -0.1
4,427,514 + dut SO4250 0.46 -1.8
4,427,514 + dut SO4250 0.46 -1.3
4,427,519 + dut SO4250 0.47 -0.4
4,427,522 - dut SO4250 0.48 -2.2
4,427,522 - dut SO4250 0.48 +0.3
4,427,522 - dut SO4250 0.48 -1.6
4,427,546 - dut SO4250 0.53 -2.3
4,427,622 + dut SO4250 0.69 -0.1
4,427,665 - dut SO4250 0.79 -1.2
4,427,791 - -3.1
4,427,795 + -1.5
4,427,840 - -0.8
4,427,840 - -0.5
4,427,863 + SO4251 0.12 -1.8
4,427,908 + SO4251 0.19 -1.4
4,427,993 + SO4251 0.34 -1.4
4,428,036 + SO4251 0.41 -0.6
4,428,057 + SO4251 0.44 -0.4
4,428,232 - SO4251 0.74 -2.0
4,428,252 + SO4251 0.77 -1.5

Or see this region's nucleotide sequence