Experiment: Cisplatin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gshB and SO0832 are separated by 93 nucleotides SO0832 and endA are separated by 8 nucleotides endA and SO0834 are separated by 138 nucleotides SO0834 and SO0835 are separated by 141 nucleotides
SO0831: gshB - glutathione synthetase (NCBI ptt file), at 849,536 to 850,483
gshB
SO0832: SO0832 - conserved hypothetical protein TIGR00046 (NCBI ptt file), at 850,577 to 851,308
SO0832
SO0833: endA - endonuclease I (NCBI ptt file), at 851,317 to 852,093
endA
SO0834: SO0834 - sprT protein, putative (NCBI ptt file), at 852,232 to 852,852
SO0834
SO0835: SO0835 - conserved hypothetical protein (NCBI ptt file), at 852,994 to 854,151
SO0835
Position (kb) 851
852
853 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 850.333 kb on + strand, within gshB at 850.397 kb on - strand at 850.462 kb on + strand at 850.529 kb on + strand at 850.622 kb on + strand at 850.630 kb on - strand at 850.630 kb on - strand at 850.632 kb on + strand at 850.640 kb on - strand at 850.666 kb on - strand, within SO0832 at 850.763 kb on - strand, within SO0832 at 850.768 kb on + strand, within SO0832 at 850.783 kb on + strand, within SO0832 at 851.007 kb on - strand, within SO0832 at 851.007 kb on - strand, within SO0832 at 851.064 kb on + strand, within SO0832 at 851.162 kb on + strand, within SO0832 at 851.183 kb on - strand, within SO0832 at 851.207 kb on - strand, within SO0832 at 851.218 kb on - strand, within SO0832 at 851.441 kb on + strand, within endA at 851.443 kb on - strand, within endA at 851.651 kb on + strand, within endA at 851.651 kb on + strand, within endA at 851.681 kb on - strand, within endA at 851.763 kb on - strand, within endA at 851.796 kb on - strand, within endA at 851.796 kb on - strand, within endA at 851.937 kb on - strand, within endA at 851.937 kb on - strand, within endA at 851.946 kb on + strand, within endA at 851.973 kb on - strand, within endA at 851.974 kb on - strand, within endA at 851.984 kb on - strand, within endA at 852.046 kb on + strand at 852.063 kb on + strand at 852.072 kb on - strand at 852.220 kb on + strand at 852.228 kb on - strand at 852.230 kb on + strand at 852.249 kb on + strand at 852.286 kb on + strand at 852.294 kb on - strand, within SO0834 at 852.392 kb on + strand, within SO0834 at 852.401 kb on - strand, within SO0834 at 852.449 kb on + strand, within SO0834 at 852.538 kb on + strand, within SO0834 at 852.556 kb on - strand, within SO0834 at 852.556 kb on - strand, within SO0834 at 852.556 kb on - strand, within SO0834 at 852.660 kb on + strand, within SO0834 at 852.707 kb on - strand, within SO0834 at 852.798 kb on + strand at 853.001 kb on - strand at 853.001 kb on - strand at 853.040 kb on + strand at 853.070 kb on + strand at 853.085 kb on - strand at 853.092 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.002 mg/ml remove 850,333 + gshB SO0831 0.84 -2.3 850,397 - -0.7 850,462 + -0.6 850,529 + -0.9 850,622 + +0.7 850,630 - +0.1 850,630 - -0.4 850,632 + -1.3 850,640 - -2.2 850,666 - SO0832 0.12 +0.5 850,763 - SO0832 0.25 +0.5 850,768 + SO0832 0.26 -0.1 850,783 + SO0832 0.28 -0.1 851,007 - SO0832 0.59 -1.1 851,007 - SO0832 0.59 -1.2 851,064 + SO0832 0.67 -1.2 851,162 + SO0832 0.80 +0.1 851,183 - SO0832 0.83 +0.5 851,207 - SO0832 0.86 +0.6 851,218 - SO0832 0.88 -0.2 851,441 + endA SO0833 0.16 -0.6 851,443 - endA SO0833 0.16 -1.1 851,651 + endA SO0833 0.43 -0.7 851,651 + endA SO0833 0.43 -0.7 851,681 - endA SO0833 0.47 -3.1 851,763 - endA SO0833 0.57 +1.3 851,796 - endA SO0833 0.62 -1.5 851,796 - endA SO0833 0.62 -1.1 851,937 - endA SO0833 0.80 -0.5 851,937 - endA SO0833 0.80 -1.8 851,946 + endA SO0833 0.81 -0.1 851,973 - endA SO0833 0.84 -0.1 851,974 - endA SO0833 0.85 -0.8 851,984 - endA SO0833 0.86 -0.1 852,046 + -2.1 852,063 + -0.9 852,072 - -0.1 852,220 + -0.2 852,228 - -0.4 852,230 + +0.6 852,249 + -0.4 852,286 + -0.4 852,294 - SO0834 0.10 -0.1 852,392 + SO0834 0.26 -1.3 852,401 - SO0834 0.27 -0.3 852,449 + SO0834 0.35 +0.9 852,538 + SO0834 0.49 -0.2 852,556 - SO0834 0.52 -0.4 852,556 - SO0834 0.52 +0.3 852,556 - SO0834 0.52 +0.4 852,660 + SO0834 0.69 +0.1 852,707 - SO0834 0.76 -0.4 852,798 + +1.1 853,001 - +0.2 853,001 - +0.7 853,040 + +1.1 853,070 + -1.0 853,085 - -0.0 853,092 - -0.3
Or see this region's nucleotide sequence