Experiment: Cobalt chloride 0.055 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ompR and envZ are separated by 40 nucleotides envZ and SO4635 are separated by 116 nucleotides
SO4633: ompR - transcriptional regulatory protein OmpR (NCBI ptt file), at 4,828,759 to 4,829,484
ompR
SO4634: envZ - osmolarity sensor protein EnvZ (NCBI ptt file), at 4,829,525 to 4,830,841
envZ
SO4635: SO4635 - methyl-accepting chemotaxis protein (NCBI ptt file), at 4,830,958 to 4,832,934
SO4635
Position (kb)
4829
4830
4831 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 4828.568 kb on + strand at 4828.568 kb on + strand at 4828.618 kb on - strand at 4828.636 kb on + strand at 4828.645 kb on - strand at 4828.647 kb on + strand at 4828.706 kb on + strand at 4828.816 kb on + strand at 4828.818 kb on + strand at 4828.818 kb on + strand at 4828.818 kb on + strand at 4828.824 kb on - strand at 4828.826 kb on - strand at 4828.826 kb on - strand at 4828.826 kb on - strand at 4828.833 kb on + strand, within ompR at 4828.837 kb on + strand, within ompR at 4828.844 kb on + strand, within ompR at 4828.845 kb on - strand, within ompR at 4828.852 kb on - strand, within ompR at 4828.863 kb on + strand, within ompR at 4828.919 kb on + strand, within ompR at 4828.925 kb on + strand, within ompR at 4828.933 kb on - strand, within ompR at 4828.933 kb on - strand, within ompR at 4828.933 kb on - strand, within ompR at 4828.943 kb on - strand, within ompR at 4828.980 kb on + strand, within ompR at 4828.980 kb on + strand, within ompR at 4828.980 kb on + strand, within ompR at 4828.988 kb on - strand, within ompR at 4828.988 kb on - strand, within ompR at 4828.988 kb on - strand, within ompR at 4828.988 kb on - strand, within ompR at 4828.988 kb on - strand, within ompR at 4829.217 kb on + strand, within ompR at 4829.225 kb on + strand, within ompR at 4829.225 kb on - strand, within ompR at 4829.231 kb on + strand, within ompR at 4829.239 kb on + strand, within ompR at 4829.239 kb on + strand, within ompR at 4829.373 kb on + strand, within ompR at 4829.374 kb on - strand, within ompR at 4829.424 kb on + strand at 4829.440 kb on + strand at 4829.499 kb on + strand at 4829.507 kb on - strand at 4829.511 kb on + strand at 4829.519 kb on - strand at 4829.519 kb on - strand at 4829.519 kb on - strand at 4829.593 kb on - strand at 4829.625 kb on - strand at 4829.666 kb on - strand, within envZ at 4829.763 kb on - strand, within envZ at 4829.768 kb on - strand, within envZ at 4829.793 kb on + strand, within envZ at 4829.820 kb on + strand, within envZ at 4829.893 kb on - strand, within envZ at 4829.901 kb on + strand, within envZ at 4829.929 kb on - strand, within envZ at 4829.929 kb on - strand, within envZ at 4829.941 kb on + strand, within envZ at 4829.956 kb on + strand, within envZ at 4829.956 kb on + strand, within envZ at 4829.956 kb on + strand, within envZ at 4829.964 kb on - strand, within envZ at 4829.999 kb on + strand, within envZ at 4830.012 kb on + strand, within envZ at 4830.180 kb on + strand, within envZ at 4830.180 kb on + strand, within envZ at 4830.180 kb on + strand, within envZ at 4830.180 kb on + strand, within envZ at 4830.188 kb on - strand, within envZ at 4830.188 kb on - strand, within envZ at 4830.230 kb on - strand, within envZ at 4830.232 kb on + strand, within envZ at 4830.240 kb on - strand, within envZ at 4830.240 kb on - strand, within envZ at 4830.266 kb on + strand, within envZ at 4830.300 kb on + strand, within envZ at 4830.406 kb on - strand, within envZ at 4830.452 kb on - strand, within envZ at 4830.462 kb on - strand, within envZ at 4830.512 kb on - strand, within envZ at 4830.687 kb on - strand, within envZ at 4830.687 kb on - strand, within envZ at 4830.705 kb on - strand, within envZ at 4830.716 kb on + strand at 4830.723 kb on + strand at 4830.724 kb on - strand at 4830.731 kb on - strand at 4830.787 kb on + strand at 4830.971 kb on - strand at 4831.004 kb on - strand at 4831.038 kb on - strand at 4831.096 kb on - strand at 4831.096 kb on - strand at 4831.151 kb on + strand at 4831.168 kb on - strand, within SO4635 at 4831.170 kb on + strand, within SO4635 at 4831.225 kb on + strand, within SO4635 at 4831.233 kb on - strand, within SO4635 at 4831.245 kb on - strand, within SO4635 at 4831.281 kb on - strand, within SO4635 at 4831.312 kb on + strand, within SO4635 at 4831.322 kb on + strand, within SO4635 at 4831.322 kb on + strand, within SO4635 at 4831.330 kb on - strand, within SO4635 at 4831.330 kb on - strand, within SO4635 at 4831.408 kb on - strand, within SO4635 at 4831.416 kb on - strand, within SO4635 at 4831.446 kb on - strand, within SO4635 at 4831.638 kb on - strand, within SO4635 at 4831.651 kb on - strand, within SO4635
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.055 mM remove 4,828,568 + -1.1 4,828,568 + +2.6 4,828,618 - +1.9 4,828,636 + -0.9 4,828,645 - +2.1 4,828,647 + -1.0 4,828,706 + -0.5 4,828,816 + +1.4 4,828,818 + +1.9 4,828,818 + +2.9 4,828,818 + +1.2 4,828,824 - +1.8 4,828,826 - +1.8 4,828,826 - +2.4 4,828,826 - +0.1 4,828,833 + ompR SO4633 0.10 +1.9 4,828,837 + ompR SO4633 0.11 +1.7 4,828,844 + ompR SO4633 0.12 +1.4 4,828,845 - ompR SO4633 0.12 +1.7 4,828,852 - ompR SO4633 0.13 +1.3 4,828,863 + ompR SO4633 0.14 +2.0 4,828,919 + ompR SO4633 0.22 +3.9 4,828,925 + ompR SO4633 0.23 +1.8 4,828,933 - ompR SO4633 0.24 +1.2 4,828,933 - ompR SO4633 0.24 +1.6 4,828,933 - ompR SO4633 0.24 +1.9 4,828,943 - ompR SO4633 0.25 +1.7 4,828,980 + ompR SO4633 0.30 +0.9 4,828,980 + ompR SO4633 0.30 +1.0 4,828,980 + ompR SO4633 0.30 +2.1 4,828,988 - ompR SO4633 0.32 +1.3 4,828,988 - ompR SO4633 0.32 +2.2 4,828,988 - ompR SO4633 0.32 +1.2 4,828,988 - ompR SO4633 0.32 +2.0 4,828,988 - ompR SO4633 0.32 +3.9 4,829,217 + ompR SO4633 0.63 +0.4 4,829,225 + ompR SO4633 0.64 +0.6 4,829,225 - ompR SO4633 0.64 +1.5 4,829,231 + ompR SO4633 0.65 +1.5 4,829,239 + ompR SO4633 0.66 +1.0 4,829,239 + ompR SO4633 0.66 +2.1 4,829,373 + ompR SO4633 0.85 +1.4 4,829,374 - ompR SO4633 0.85 +1.0 4,829,424 + +0.4 4,829,440 + +1.1 4,829,499 + -1.1 4,829,507 - +1.8 4,829,511 + -0.8 4,829,519 - +3.1 4,829,519 - -1.3 4,829,519 - +0.7 4,829,593 - -0.5 4,829,625 - -0.6 4,829,666 - envZ SO4634 0.11 +1.5 4,829,763 - envZ SO4634 0.18 +2.4 4,829,768 - envZ SO4634 0.18 -0.1 4,829,793 + envZ SO4634 0.20 -0.8 4,829,820 + envZ SO4634 0.22 +1.7 4,829,893 - envZ SO4634 0.28 +0.9 4,829,901 + envZ SO4634 0.29 +0.6 4,829,929 - envZ SO4634 0.31 +0.4 4,829,929 - envZ SO4634 0.31 +1.9 4,829,941 + envZ SO4634 0.32 +0.7 4,829,956 + envZ SO4634 0.33 +0.9 4,829,956 + envZ SO4634 0.33 +2.2 4,829,956 + envZ SO4634 0.33 +2.0 4,829,964 - envZ SO4634 0.33 +6.9 4,829,999 + envZ SO4634 0.36 +2.2 4,830,012 + envZ SO4634 0.37 +1.2 4,830,180 + envZ SO4634 0.50 +0.2 4,830,180 + envZ SO4634 0.50 +0.6 4,830,180 + envZ SO4634 0.50 +1.5 4,830,180 + envZ SO4634 0.50 +1.3 4,830,188 - envZ SO4634 0.50 +4.2 4,830,188 - envZ SO4634 0.50 +2.7 4,830,230 - envZ SO4634 0.54 +1.4 4,830,232 + envZ SO4634 0.54 +1.3 4,830,240 - envZ SO4634 0.54 +0.5 4,830,240 - envZ SO4634 0.54 +2.0 4,830,266 + envZ SO4634 0.56 -0.5 4,830,300 + envZ SO4634 0.59 +1.3 4,830,406 - envZ SO4634 0.67 +1.6 4,830,452 - envZ SO4634 0.70 +0.8 4,830,462 - envZ SO4634 0.71 +1.9 4,830,512 - envZ SO4634 0.75 +2.9 4,830,687 - envZ SO4634 0.88 +1.1 4,830,687 - envZ SO4634 0.88 +0.3 4,830,705 - envZ SO4634 0.90 +1.8 4,830,716 + +2.7 4,830,723 + +1.9 4,830,724 - +1.3 4,830,731 - +1.8 4,830,787 + +2.0 4,830,971 - +1.3 4,831,004 - +0.1 4,831,038 - -2.2 4,831,096 - -0.2 4,831,096 - -0.4 4,831,151 + +0.8 4,831,168 - SO4635 0.11 -0.1 4,831,170 + SO4635 0.11 +1.1 4,831,225 + SO4635 0.14 -2.2 4,831,233 - SO4635 0.14 -1.2 4,831,245 - SO4635 0.15 +1.3 4,831,281 - SO4635 0.16 -0.3 4,831,312 + SO4635 0.18 -0.5 4,831,322 + SO4635 0.18 -0.4 4,831,322 + SO4635 0.18 +1.5 4,831,330 - SO4635 0.19 -1.8 4,831,330 - SO4635 0.19 -0.9 4,831,408 - SO4635 0.23 -1.8 4,831,416 - SO4635 0.23 +0.3 4,831,446 - SO4635 0.25 -0.4 4,831,638 - SO4635 0.34 -3.1 4,831,651 - SO4635 0.35 -0.3
Or see this region's nucleotide sequence