Strain Fitness in Shewanella oneidensis MR-1 around SO3106

Experiment: Cobalt chloride 0.055 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpspE-1 and aprE are separated by 576 nucleotidesaprE and SO3107 are separated by 110 nucleotidesSO3107 and SO3108 are separated by 85 nucleotides SO3105: pspE-1 - phage shock protein E (NCBI ptt file), at 3,232,563 to 3,232,961 pspE-1 SO3106: aprE - cold-active serine alkaline protease (NCBI ptt file), at 3,233,538 to 3,235,961 aprE SO3107: SO3107 - conserved hypothetical protein (NCBI ptt file), at 3,236,072 to 3,236,617 SO3107 SO3108: SO3108 - siroheme synthase, N-terminal component, putative (NCBI ptt file), at 3,236,703 to 3,237,614 SO3108 Position (kb) 3233 3234 3235 3236Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6at 3232.729 kb on - strand, within pspE-1at 3232.767 kb on + strand, within pspE-1at 3232.922 kb on - strandat 3232.964 kb on - strandat 3233.056 kb on - strandat 3233.057 kb on + strandat 3233.581 kb on + strandat 3233.783 kb on - strand, within aprEat 3233.804 kb on - strand, within aprEat 3233.887 kb on + strand, within aprEat 3233.921 kb on - strand, within aprEat 3233.983 kb on + strand, within aprEat 3234.048 kb on + strand, within aprEat 3234.048 kb on + strand, within aprEat 3234.056 kb on - strand, within aprEat 3234.056 kb on - strand, within aprEat 3234.056 kb on - strand, within aprEat 3234.071 kb on - strand, within aprEat 3234.264 kb on - strand, within aprEat 3234.269 kb on - strand, within aprEat 3234.284 kb on + strand, within aprEat 3234.294 kb on + strand, within aprEat 3234.294 kb on + strand, within aprEat 3234.573 kb on + strand, within aprEat 3234.755 kb on - strand, within aprEat 3234.957 kb on + strand, within aprEat 3235.160 kb on + strand, within aprEat 3235.229 kb on - strand, within aprEat 3235.295 kb on + strand, within aprEat 3235.317 kb on + strand, within aprEat 3235.400 kb on - strand, within aprEat 3235.438 kb on + strand, within aprEat 3235.438 kb on + strand, within aprEat 3235.541 kb on + strand, within aprEat 3235.585 kb on + strand, within aprEat 3235.585 kb on + strand, within aprEat 3235.585 kb on + strand, within aprEat 3235.658 kb on - strand, within aprEat 3235.679 kb on - strand, within aprEat 3235.800 kb on + strandat 3235.808 kb on + strandat 3235.844 kb on + strandat 3235.852 kb on - strandat 3235.852 kb on - strandat 3235.867 kb on + strandat 3236.009 kb on + strandat 3236.125 kb on - strandat 3236.254 kb on - strand, within SO3107at 3236.266 kb on + strand, within SO3107at 3236.405 kb on + strand, within SO3107at 3236.589 kb on + strandat 3236.597 kb on - strandat 3236.623 kb on + strandat 3236.735 kb on - strandat 3236.746 kb on + strandat 3236.754 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.055 mM
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3,232,729 - pspE-1 SO3105 0.42 -0.3
3,232,767 + pspE-1 SO3105 0.51 -1.3
3,232,922 - -0.5
3,232,964 - -1.1
3,233,056 - +2.9
3,233,057 + -0.2
3,233,581 + +2.4
3,233,783 - aprE SO3106 0.10 +1.1
3,233,804 - aprE SO3106 0.11 +1.3
3,233,887 + aprE SO3106 0.14 -0.6
3,233,921 - aprE SO3106 0.16 +0.4
3,233,983 + aprE SO3106 0.18 -0.9
3,234,048 + aprE SO3106 0.21 +5.6
3,234,048 + aprE SO3106 0.21 -0.1
3,234,056 - aprE SO3106 0.21 -0.4
3,234,056 - aprE SO3106 0.21 -0.3
3,234,056 - aprE SO3106 0.21 +0.1
3,234,071 - aprE SO3106 0.22 +6.0
3,234,264 - aprE SO3106 0.30 -0.0
3,234,269 - aprE SO3106 0.30 -0.7
3,234,284 + aprE SO3106 0.31 -0.4
3,234,294 + aprE SO3106 0.31 -0.9
3,234,294 + aprE SO3106 0.31 +0.1
3,234,573 + aprE SO3106 0.43 +0.9
3,234,755 - aprE SO3106 0.50 -1.0
3,234,957 + aprE SO3106 0.59 -1.6
3,235,160 + aprE SO3106 0.67 -0.4
3,235,229 - aprE SO3106 0.70 +0.3
3,235,295 + aprE SO3106 0.72 -0.2
3,235,317 + aprE SO3106 0.73 +0.3
3,235,400 - aprE SO3106 0.77 -0.6
3,235,438 + aprE SO3106 0.78 -0.1
3,235,438 + aprE SO3106 0.78 -1.4
3,235,541 + aprE SO3106 0.83 -0.3
3,235,585 + aprE SO3106 0.84 -0.2
3,235,585 + aprE SO3106 0.84 +1.7
3,235,585 + aprE SO3106 0.84 +0.7
3,235,658 - aprE SO3106 0.87 +0.2
3,235,679 - aprE SO3106 0.88 +0.7
3,235,800 + -1.9
3,235,808 + +0.4
3,235,844 + -1.6
3,235,852 - +0.3
3,235,852 - -0.9
3,235,867 + -0.6
3,236,009 + -2.4
3,236,125 - +0.4
3,236,254 - SO3107 0.33 +3.5
3,236,266 + SO3107 0.36 +0.3
3,236,405 + SO3107 0.61 +1.1
3,236,589 + -2.0
3,236,597 - +0.3
3,236,623 + +0.1
3,236,735 - -0.9
3,236,746 + +0.4
3,236,754 - +0.4

Or see this region's nucleotide sequence