Experiment: Cobalt chloride 0.055 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tesB and SO1913 are separated by 7 nucleotides SO1913 and SO1915 are separated by 575 nucleotides SO1915 and SO1916 are separated by 92 nucleotides
SO1912: tesB - acyl-CoA thioesterase II (NCBI ptt file), at 2,010,600 to 2,011,466
tesB
SO1913: SO1913 - conserved hypothetical protein (NCBI ptt file), at 2,011,474 to 2,011,872
SO1913
SO1915: SO1915 - serine protease, subtilase family (NCBI ptt file), at 2,012,448 to 2,016,350
SO1915
SO1916: SO1916 - transcriptional regulator, LysR family (NCBI ptt file), at 2,016,443 to 2,017,324
SO1916
Position (kb)
2012
2013
2014
2015
2016
2017 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9 at 2011.541 kb on + strand, within SO1913 at 2011.544 kb on - strand, within SO1913 at 2011.550 kb on - strand, within SO1913 at 2011.597 kb on - strand, within SO1913 at 2011.607 kb on - strand, within SO1913 at 2011.832 kb on + strand, within SO1913 at 2011.847 kb on - strand at 2011.854 kb on + strand at 2011.862 kb on - strand at 2011.862 kb on - strand at 2011.862 kb on - strand at 2011.862 kb on - strand at 2011.862 kb on - strand at 2011.862 kb on - strand at 2011.862 kb on - strand at 2011.926 kb on + strand at 2011.934 kb on - strand at 2011.937 kb on - strand at 2011.985 kb on - strand at 2012.140 kb on - strand at 2012.155 kb on + strand at 2012.486 kb on + strand at 2012.486 kb on + strand at 2012.494 kb on - strand at 2012.494 kb on - strand at 2012.533 kb on + strand at 2012.572 kb on + strand at 2012.659 kb on - strand at 2012.719 kb on - strand at 2012.719 kb on - strand at 2012.719 kb on - strand at 2012.762 kb on - strand at 2012.862 kb on + strand, within SO1915 at 2012.883 kb on + strand, within SO1915 at 2012.893 kb on + strand, within SO1915 at 2012.913 kb on - strand, within SO1915 at 2012.992 kb on + strand, within SO1915 at 2013.043 kb on + strand, within SO1915 at 2013.103 kb on - strand, within SO1915 at 2013.168 kb on - strand, within SO1915 at 2013.191 kb on - strand, within SO1915 at 2013.252 kb on + strand, within SO1915 at 2013.262 kb on + strand, within SO1915 at 2013.262 kb on + strand, within SO1915 at 2013.262 kb on + strand, within SO1915 at 2013.270 kb on - strand, within SO1915 at 2013.289 kb on + strand, within SO1915 at 2013.349 kb on + strand, within SO1915 at 2013.400 kb on + strand, within SO1915 at 2013.430 kb on - strand, within SO1915 at 2013.430 kb on - strand, within SO1915 at 2013.435 kb on - strand, within SO1915 at 2013.595 kb on + strand, within SO1915 at 2013.649 kb on - strand, within SO1915 at 2013.662 kb on - strand, within SO1915 at 2013.749 kb on - strand, within SO1915 at 2013.758 kb on + strand, within SO1915 at 2013.824 kb on + strand, within SO1915 at 2013.842 kb on - strand, within SO1915 at 2013.872 kb on + strand, within SO1915 at 2013.872 kb on + strand, within SO1915 at 2013.882 kb on + strand, within SO1915 at 2013.882 kb on + strand, within SO1915 at 2013.890 kb on - strand, within SO1915 at 2013.951 kb on + strand, within SO1915 at 2014.015 kb on + strand, within SO1915 at 2014.038 kb on + strand, within SO1915 at 2014.038 kb on + strand, within SO1915 at 2014.091 kb on - strand, within SO1915 at 2014.091 kb on - strand, within SO1915 at 2014.098 kb on + strand, within SO1915 at 2014.106 kb on - strand, within SO1915 at 2014.121 kb on - strand, within SO1915 at 2014.170 kb on - strand, within SO1915 at 2014.225 kb on - strand, within SO1915 at 2014.277 kb on - strand, within SO1915 at 2014.426 kb on + strand, within SO1915 at 2014.480 kb on - strand, within SO1915 at 2014.485 kb on + strand, within SO1915 at 2014.490 kb on + strand, within SO1915 at 2014.493 kb on - strand, within SO1915 at 2014.498 kb on - strand, within SO1915 at 2014.508 kb on - strand, within SO1915 at 2014.663 kb on - strand, within SO1915 at 2014.724 kb on + strand, within SO1915 at 2014.747 kb on + strand, within SO1915 at 2014.747 kb on - strand, within SO1915 at 2014.779 kb on + strand, within SO1915 at 2014.884 kb on + strand, within SO1915 at 2014.899 kb on + strand, within SO1915 at 2015.028 kb on - strand, within SO1915 at 2015.045 kb on + strand, within SO1915 at 2015.115 kb on - strand, within SO1915 at 2015.117 kb on - strand, within SO1915 at 2015.149 kb on - strand, within SO1915 at 2015.156 kb on - strand, within SO1915 at 2015.186 kb on - strand, within SO1915 at 2015.331 kb on - strand, within SO1915 at 2015.402 kb on + strand, within SO1915 at 2015.422 kb on - strand, within SO1915 at 2015.452 kb on - strand, within SO1915 at 2015.503 kb on + strand, within SO1915 at 2015.550 kb on - strand, within SO1915 at 2015.679 kb on + strand, within SO1915 at 2015.724 kb on + strand, within SO1915 at 2015.735 kb on + strand, within SO1915 at 2015.743 kb on + strand, within SO1915 at 2015.758 kb on + strand, within SO1915 at 2015.866 kb on - strand, within SO1915 at 2015.887 kb on + strand, within SO1915 at 2016.107 kb on - strand at 2016.151 kb on + strand at 2016.288 kb on + strand at 2016.296 kb on - strand at 2016.334 kb on + strand at 2016.352 kb on - strand at 2016.385 kb on + strand at 2016.404 kb on - strand at 2016.560 kb on + strand, within SO1916 at 2016.560 kb on + strand, within SO1916 at 2016.568 kb on - strand, within SO1916 at 2016.684 kb on + strand, within SO1916 at 2016.734 kb on - strand, within SO1916 at 2016.734 kb on - strand, within SO1916 at 2016.734 kb on - strand, within SO1916 at 2016.825 kb on - strand, within SO1916 at 2016.978 kb on + strand, within SO1916 at 2016.986 kb on - strand, within SO1916 at 2017.118 kb on + strand, within SO1916 at 2017.123 kb on + strand, within SO1916 at 2017.126 kb on - strand, within SO1916 at 2017.132 kb on - strand, within SO1916 at 2017.132 kb on - strand, within SO1916 at 2017.138 kb on - strand, within SO1916 at 2017.143 kb on + strand, within SO1916 at 2017.178 kb on + strand, within SO1916 at 2017.186 kb on + strand, within SO1916 at 2017.236 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.055 mM remove 2,011,541 + SO1913 0.17 -0.9 2,011,544 - SO1913 0.18 +0.3 2,011,550 - SO1913 0.19 -0.2 2,011,597 - SO1913 0.31 -0.2 2,011,607 - SO1913 0.33 +0.3 2,011,832 + SO1913 0.90 -2.2 2,011,847 - -0.5 2,011,854 + -1.4 2,011,862 - +0.8 2,011,862 - -2.3 2,011,862 - +1.9 2,011,862 - +0.2 2,011,862 - -2.0 2,011,862 - +0.4 2,011,862 - -1.9 2,011,926 + -0.4 2,011,934 - +0.1 2,011,937 - +0.1 2,011,985 - -1.9 2,012,140 - +0.5 2,012,155 + -0.4 2,012,486 + +0.7 2,012,486 + -1.2 2,012,494 - +0.7 2,012,494 - +1.7 2,012,533 + -1.6 2,012,572 + +0.2 2,012,659 - -2.5 2,012,719 - +0.5 2,012,719 - -0.4 2,012,719 - -0.3 2,012,762 - +0.9 2,012,862 + SO1915 0.11 -0.7 2,012,883 + SO1915 0.11 -0.2 2,012,893 + SO1915 0.11 +1.0 2,012,913 - SO1915 0.12 -1.9 2,012,992 + SO1915 0.14 +0.7 2,013,043 + SO1915 0.15 +0.1 2,013,103 - SO1915 0.17 -1.3 2,013,168 - SO1915 0.18 +0.7 2,013,191 - SO1915 0.19 +0.4 2,013,252 + SO1915 0.21 +0.7 2,013,262 + SO1915 0.21 +0.8 2,013,262 + SO1915 0.21 +0.4 2,013,262 + SO1915 0.21 -2.1 2,013,270 - SO1915 0.21 +3.5 2,013,289 + SO1915 0.22 +0.1 2,013,349 + SO1915 0.23 +0.5 2,013,400 + SO1915 0.24 +0.8 2,013,430 - SO1915 0.25 +1.3 2,013,430 - SO1915 0.25 +0.7 2,013,435 - SO1915 0.25 +1.3 2,013,595 + SO1915 0.29 +0.4 2,013,649 - SO1915 0.31 -0.9 2,013,662 - SO1915 0.31 +1.4 2,013,749 - SO1915 0.33 +0.4 2,013,758 + SO1915 0.34 -0.1 2,013,824 + SO1915 0.35 +1.0 2,013,842 - SO1915 0.36 -0.2 2,013,872 + SO1915 0.36 +4.5 2,013,872 + SO1915 0.36 -2.2 2,013,882 + SO1915 0.37 +0.1 2,013,882 + SO1915 0.37 +0.1 2,013,890 - SO1915 0.37 +0.6 2,013,951 + SO1915 0.39 +6.0 2,014,015 + SO1915 0.40 -0.1 2,014,038 + SO1915 0.41 +9.4 2,014,038 + SO1915 0.41 -1.1 2,014,091 - SO1915 0.42 -1.1 2,014,091 - SO1915 0.42 -1.7 2,014,098 + SO1915 0.42 +1.7 2,014,106 - SO1915 0.42 -0.5 2,014,121 - SO1915 0.43 -1.6 2,014,170 - SO1915 0.44 +0.5 2,014,225 - SO1915 0.46 +0.1 2,014,277 - SO1915 0.47 -0.8 2,014,426 + SO1915 0.51 +1.2 2,014,480 - SO1915 0.52 +3.5 2,014,485 + SO1915 0.52 -2.2 2,014,490 + SO1915 0.52 +1.3 2,014,493 - SO1915 0.52 -0.1 2,014,498 - SO1915 0.53 -0.4 2,014,508 - SO1915 0.53 -0.3 2,014,663 - SO1915 0.57 -2.3 2,014,724 + SO1915 0.58 +0.1 2,014,747 + SO1915 0.59 +0.2 2,014,747 - SO1915 0.59 -0.4 2,014,779 + SO1915 0.60 +0.6 2,014,884 + SO1915 0.62 -0.2 2,014,899 + SO1915 0.63 +1.0 2,015,028 - SO1915 0.66 +3.7 2,015,045 + SO1915 0.67 -0.5 2,015,115 - SO1915 0.68 -1.2 2,015,117 - SO1915 0.68 -1.4 2,015,149 - SO1915 0.69 +1.1 2,015,156 - SO1915 0.69 -1.1 2,015,186 - SO1915 0.70 +0.5 2,015,331 - SO1915 0.74 -1.8 2,015,402 + SO1915 0.76 -0.6 2,015,422 - SO1915 0.76 -0.7 2,015,452 - SO1915 0.77 +2.5 2,015,503 + SO1915 0.78 +1.9 2,015,550 - SO1915 0.79 +1.6 2,015,679 + SO1915 0.83 -2.3 2,015,724 + SO1915 0.84 -0.7 2,015,735 + SO1915 0.84 +0.0 2,015,743 + SO1915 0.84 +0.6 2,015,758 + SO1915 0.85 +2.3 2,015,866 - SO1915 0.88 +0.1 2,015,887 + SO1915 0.88 +0.7 2,016,107 - -0.5 2,016,151 + +3.1 2,016,288 + -0.4 2,016,296 - -1.6 2,016,334 + +0.6 2,016,352 - +0.2 2,016,385 + -0.9 2,016,404 - +0.3 2,016,560 + SO1916 0.13 +0.2 2,016,560 + SO1916 0.13 +1.4 2,016,568 - SO1916 0.14 +0.1 2,016,684 + SO1916 0.27 -0.2 2,016,734 - SO1916 0.33 +0.4 2,016,734 - SO1916 0.33 +0.8 2,016,734 - SO1916 0.33 -2.3 2,016,825 - SO1916 0.43 +1.0 2,016,978 + SO1916 0.61 +2.3 2,016,986 - SO1916 0.62 +0.1 2,017,118 + SO1916 0.77 -1.6 2,017,123 + SO1916 0.77 -2.4 2,017,126 - SO1916 0.77 +0.0 2,017,132 - SO1916 0.78 -0.4 2,017,132 - SO1916 0.78 -0.2 2,017,138 - SO1916 0.79 +1.1 2,017,143 + SO1916 0.79 -0.6 2,017,178 + SO1916 0.83 -0.4 2,017,186 + SO1916 0.84 +4.2 2,017,236 + -0.2
Or see this region's nucleotide sequence