Strain Fitness in Shewanella oneidensis MR-1 around SO0486

Experiment: Cobalt chloride 0.055 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnrfD and nosL are separated by 119 nucleotidesnosL and nosD are separated by 10 nucleotidesnosD and nosF are separated by 44 nucleotides SO0484: nrfD - formate-dependent nitrite reductase, nrfD protein (NCBI ptt file), at 507,756 to 508,691 nrfD SO0485: nosL - nosL protein (NCBI ptt file), at 508,811 to 509,302 nosL SO0486: nosD - copper ABC transporter, periplasmic copper-binding protein (NCBI ptt file), at 509,313 to 510,587 nosD SO0487: nosF - copper ABC transporter, ATP-binding protein (NCBI ptt file), at 510,632 to 511,630 nosF Position (kb) 509 510 511Strain fitness (log2 ratio) -2 -1 0 1 2 3at 508.316 kb on - strand, within nrfDat 508.353 kb on + strand, within nrfDat 508.353 kb on + strand, within nrfDat 508.361 kb on - strand, within nrfDat 508.387 kb on + strand, within nrfDat 508.387 kb on + strand, within nrfDat 508.387 kb on + strand, within nrfDat 508.387 kb on + strand, within nrfDat 508.443 kb on + strand, within nrfDat 508.490 kb on + strand, within nrfDat 508.490 kb on + strand, within nrfDat 508.602 kb on + strandat 508.622 kb on + strandat 508.637 kb on - strandat 508.645 kb on + strandat 508.645 kb on - strandat 508.653 kb on - strandat 508.661 kb on - strandat 508.696 kb on + strandat 508.743 kb on + strandat 508.769 kb on - strandat 508.928 kb on - strand, within nosLat 508.978 kb on + strand, within nosLat 508.978 kb on + strand, within nosLat 508.978 kb on + strand, within nosLat 508.978 kb on + strand, within nosLat 508.978 kb on + strand, within nosLat 508.978 kb on + strand, within nosLat 508.978 kb on + strand, within nosLat 508.978 kb on + strand, within nosLat 508.978 kb on + strand, within nosLat 508.986 kb on - strand, within nosLat 508.986 kb on - strand, within nosLat 508.986 kb on - strand, within nosLat 508.986 kb on - strand, within nosLat 508.986 kb on - strand, within nosLat 508.986 kb on - strand, within nosLat 508.986 kb on - strand, within nosLat 509.024 kb on + strand, within nosLat 509.251 kb on + strand, within nosLat 509.252 kb on + strand, within nosLat 509.252 kb on + strand, within nosLat 509.252 kb on + strand, within nosLat 509.252 kb on + strand, within nosLat 509.260 kb on - strandat 509.260 kb on - strandat 509.260 kb on - strandat 509.267 kb on - strandat 509.274 kb on + strandat 509.382 kb on - strandat 509.420 kb on + strandat 509.589 kb on - strand, within nosDat 509.650 kb on + strand, within nosDat 509.655 kb on - strand, within nosDat 509.820 kb on - strand, within nosDat 509.869 kb on - strand, within nosDat 509.871 kb on + strand, within nosDat 509.925 kb on + strand, within nosDat 509.945 kb on + strand, within nosDat 509.994 kb on - strand, within nosDat 510.077 kb on + strand, within nosDat 510.103 kb on + strand, within nosDat 510.127 kb on + strand, within nosDat 510.127 kb on - strand, within nosDat 510.127 kb on - strand, within nosDat 510.179 kb on + strand, within nosDat 510.212 kb on + strand, within nosDat 510.234 kb on + strand, within nosDat 510.291 kb on - strand, within nosDat 510.306 kb on + strand, within nosDat 510.360 kb on - strand, within nosDat 510.388 kb on + strand, within nosDat 510.396 kb on - strand, within nosDat 510.396 kb on - strand, within nosDat 510.411 kb on + strand, within nosDat 510.419 kb on - strand, within nosDat 510.480 kb on - strandat 510.491 kb on + strandat 510.620 kb on - strandat 510.791 kb on - strand, within nosFat 510.849 kb on + strand, within nosFat 510.972 kb on - strand, within nosFat 510.980 kb on - strand, within nosFat 511.030 kb on + strand, within nosFat 511.038 kb on - strand, within nosFat 511.070 kb on + strand, within nosFat 511.078 kb on - strand, within nosFat 511.109 kb on - strand, within nosFat 511.137 kb on + strand, within nosFat 511.145 kb on - strand, within nosFat 511.190 kb on + strand, within nosFat 511.198 kb on - strand, within nosFat 511.198 kb on - strand, within nosFat 511.274 kb on + strand, within nosFat 511.303 kb on - strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.305 kb on + strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.313 kb on - strand, within nosFat 511.412 kb on + strand, within nosFat 511.418 kb on + strand, within nosFat 511.418 kb on + strand, within nosFat 511.418 kb on + strand, within nosFat 511.426 kb on - strand, within nosFat 511.434 kb on + strand, within nosFat 511.434 kb on + strand, within nosFat 511.434 kb on + strand, within nosFat 511.434 kb on + strand, within nosFat 511.442 kb on - strand, within nosFat 511.442 kb on - strand, within nosFat 511.554 kb on + strandat 511.581 kb on - strandat 511.586 kb on + strandat 511.586 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.055 mM
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508,316 - nrfD SO0484 0.60 -0.2
508,353 + nrfD SO0484 0.64 -0.4
508,353 + nrfD SO0484 0.64 +0.6
508,361 - nrfD SO0484 0.65 -0.9
508,387 + nrfD SO0484 0.67 +0.6
508,387 + nrfD SO0484 0.67 +0.3
508,387 + nrfD SO0484 0.67 -0.4
508,387 + nrfD SO0484 0.67 -0.7
508,443 + nrfD SO0484 0.73 +0.7
508,490 + nrfD SO0484 0.78 -2.7
508,490 + nrfD SO0484 0.78 +0.5
508,602 + +1.4
508,622 + -1.4
508,637 - +0.4
508,645 + +0.1
508,645 - -0.2
508,653 - +1.8
508,661 - +0.6
508,696 + -1.8
508,743 + +0.6
508,769 - -1.2
508,928 - nosL SO0485 0.24 -1.3
508,978 + nosL SO0485 0.34 -0.8
508,978 + nosL SO0485 0.34 +1.1
508,978 + nosL SO0485 0.34 +0.9
508,978 + nosL SO0485 0.34 -1.3
508,978 + nosL SO0485 0.34 -1.4
508,978 + nosL SO0485 0.34 -0.3
508,978 + nosL SO0485 0.34 -2.0
508,978 + nosL SO0485 0.34 -0.6
508,978 + nosL SO0485 0.34 +0.1
508,986 - nosL SO0485 0.36 -0.4
508,986 - nosL SO0485 0.36 -0.9
508,986 - nosL SO0485 0.36 +0.7
508,986 - nosL SO0485 0.36 -1.4
508,986 - nosL SO0485 0.36 -1.0
508,986 - nosL SO0485 0.36 -0.0
508,986 - nosL SO0485 0.36 +0.2
509,024 + nosL SO0485 0.43 +0.3
509,251 + nosL SO0485 0.89 -0.5
509,252 + nosL SO0485 0.90 +0.7
509,252 + nosL SO0485 0.90 +1.0
509,252 + nosL SO0485 0.90 -1.5
509,252 + nosL SO0485 0.90 -0.4
509,260 - +2.4
509,260 - -1.7
509,260 - -0.6
509,267 - +0.6
509,274 + +1.1
509,382 - -0.5
509,420 + +0.0
509,589 - nosD SO0486 0.22 +1.1
509,650 + nosD SO0486 0.26 +0.4
509,655 - nosD SO0486 0.27 +0.6
509,820 - nosD SO0486 0.40 +1.9
509,869 - nosD SO0486 0.44 -1.0
509,871 + nosD SO0486 0.44 +0.3
509,925 + nosD SO0486 0.48 +0.1
509,945 + nosD SO0486 0.50 -1.1
509,994 - nosD SO0486 0.53 -0.2
510,077 + nosD SO0486 0.60 -0.1
510,103 + nosD SO0486 0.62 -2.1
510,127 + nosD SO0486 0.64 +0.6
510,127 - nosD SO0486 0.64 +0.4
510,127 - nosD SO0486 0.64 +2.0
510,179 + nosD SO0486 0.68 -0.2
510,212 + nosD SO0486 0.71 +1.8
510,234 + nosD SO0486 0.72 +0.3
510,291 - nosD SO0486 0.77 +0.1
510,306 + nosD SO0486 0.78 -0.7
510,360 - nosD SO0486 0.82 +0.2
510,388 + nosD SO0486 0.84 -0.3
510,396 - nosD SO0486 0.85 +2.8
510,396 - nosD SO0486 0.85 -0.5
510,411 + nosD SO0486 0.86 -1.3
510,419 - nosD SO0486 0.87 +1.6
510,480 - -0.5
510,491 + -0.2
510,620 - +0.1
510,791 - nosF SO0487 0.16 -1.0
510,849 + nosF SO0487 0.22 -0.1
510,972 - nosF SO0487 0.34 -0.7
510,980 - nosF SO0487 0.35 +0.1
511,030 + nosF SO0487 0.40 -0.2
511,038 - nosF SO0487 0.41 -0.1
511,070 + nosF SO0487 0.44 +0.6
511,078 - nosF SO0487 0.45 -1.1
511,109 - nosF SO0487 0.48 +0.4
511,137 + nosF SO0487 0.51 -2.5
511,145 - nosF SO0487 0.51 +1.0
511,190 + nosF SO0487 0.56 +0.3
511,198 - nosF SO0487 0.57 -1.0
511,198 - nosF SO0487 0.57 -1.3
511,274 + nosF SO0487 0.64 -0.5
511,303 - nosF SO0487 0.67 -1.8
511,305 + nosF SO0487 0.67 -1.2
511,305 + nosF SO0487 0.67 +1.1
511,305 + nosF SO0487 0.67 +1.0
511,305 + nosF SO0487 0.67 -0.4
511,305 + nosF SO0487 0.67 +0.2
511,305 + nosF SO0487 0.67 +2.9
511,305 + nosF SO0487 0.67 +1.1
511,305 + nosF SO0487 0.67 +0.3
511,305 + nosF SO0487 0.67 -1.0
511,305 + nosF SO0487 0.67 +0.1
511,305 + nosF SO0487 0.67 -1.3
511,305 + nosF SO0487 0.67 +2.8
511,305 + nosF SO0487 0.67 +0.6
511,305 + nosF SO0487 0.67 +0.4
511,305 + nosF SO0487 0.67 +0.1
511,305 + nosF SO0487 0.67 +2.3
511,305 + nosF SO0487 0.67 +1.0
511,305 + nosF SO0487 0.67 -1.3
511,305 + nosF SO0487 0.67 -0.5
511,305 + nosF SO0487 0.67 +1.0
511,305 + nosF SO0487 0.67 +0.4
511,305 + nosF SO0487 0.67 -1.9
511,305 + nosF SO0487 0.67 -0.9
511,305 + nosF SO0487 0.67 +0.8
511,305 + nosF SO0487 0.67 +1.1
511,305 + nosF SO0487 0.67 +1.5
511,313 - nosF SO0487 0.68 -1.5
511,313 - nosF SO0487 0.68 -0.8
511,313 - nosF SO0487 0.68 +1.6
511,313 - nosF SO0487 0.68 +0.2
511,313 - nosF SO0487 0.68 +1.5
511,313 - nosF SO0487 0.68 -0.4
511,313 - nosF SO0487 0.68 -1.9
511,313 - nosF SO0487 0.68 +1.2
511,313 - nosF SO0487 0.68 -0.9
511,313 - nosF SO0487 0.68 -0.8
511,313 - nosF SO0487 0.68 -0.1
511,313 - nosF SO0487 0.68 +0.4
511,313 - nosF SO0487 0.68 -0.1
511,313 - nosF SO0487 0.68 -0.2
511,313 - nosF SO0487 0.68 -0.2
511,313 - nosF SO0487 0.68 -1.7
511,313 - nosF SO0487 0.68 +1.8
511,313 - nosF SO0487 0.68 -1.7
511,313 - nosF SO0487 0.68 +0.8
511,313 - nosF SO0487 0.68 -0.8
511,313 - nosF SO0487 0.68 +0.1
511,313 - nosF SO0487 0.68 -1.6
511,313 - nosF SO0487 0.68 +0.8
511,313 - nosF SO0487 0.68 -1.5
511,313 - nosF SO0487 0.68 +0.3
511,313 - nosF SO0487 0.68 +0.1
511,412 + nosF SO0487 0.78 -0.4
511,418 + nosF SO0487 0.79 -1.4
511,418 + nosF SO0487 0.79 -0.6
511,418 + nosF SO0487 0.79 +2.4
511,426 - nosF SO0487 0.79 -1.2
511,434 + nosF SO0487 0.80 +0.4
511,434 + nosF SO0487 0.80 +0.6
511,434 + nosF SO0487 0.80 -0.1
511,434 + nosF SO0487 0.80 -0.6
511,442 - nosF SO0487 0.81 -1.7
511,442 - nosF SO0487 0.81 -1.0
511,554 + +0.1
511,581 - -0.3
511,586 + -1.5
511,586 + +0.0

Or see this region's nucleotide sequence