Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_61 and HP15_62 are separated by 41 nucleotides HP15_62 and HP15_63 are separated by 63 nucleotides HP15_63 and HP15_64 are separated by 17 nucleotides
HP15_61: HP15_61 - chain A, alpha-keto acid dehydrogenase-like protein, at 65,530 to 66,759
_61
HP15_62: HP15_62 - hypothetical protein, at 66,801 to 66,905
_62
HP15_63: HP15_63 - 2-oxoisovalerate dehydrogenase, E1 component, beta subunit, at 66,969 to 67,775
_63
HP15_64: HP15_64 - catalytic domain of components of various dehydrogenase complexes, at 67,793 to 69,091
_64
Position (kb)
66
67 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 65.876 kb on - strand, within HP15_61 at 65.896 kb on - strand, within HP15_61 at 65.898 kb on + strand, within HP15_61 at 65.906 kb on - strand, within HP15_61 at 65.906 kb on - strand, within HP15_61 at 65.906 kb on - strand, within HP15_61 at 65.942 kb on + strand, within HP15_61 at 65.950 kb on - strand, within HP15_61 at 65.950 kb on - strand, within HP15_61 at 65.954 kb on + strand, within HP15_61 at 66.025 kb on + strand, within HP15_61 at 66.105 kb on + strand, within HP15_61 at 66.105 kb on + strand, within HP15_61 at 66.105 kb on + strand, within HP15_61 at 66.105 kb on + strand, within HP15_61 at 66.105 kb on + strand, within HP15_61 at 66.113 kb on - strand, within HP15_61 at 66.113 kb on - strand, within HP15_61 at 66.113 kb on - strand, within HP15_61 at 66.113 kb on - strand, within HP15_61 at 66.113 kb on - strand, within HP15_61 at 66.161 kb on - strand, within HP15_61 at 66.192 kb on - strand, within HP15_61 at 66.199 kb on + strand, within HP15_61 at 66.224 kb on - strand, within HP15_61 at 66.225 kb on - strand, within HP15_61 at 66.230 kb on + strand, within HP15_61 at 66.283 kb on - strand, within HP15_61 at 66.447 kb on + strand, within HP15_61 at 66.472 kb on + strand, within HP15_61 at 66.489 kb on + strand, within HP15_61 at 66.489 kb on + strand, within HP15_61 at 66.489 kb on + strand, within HP15_61 at 66.497 kb on - strand, within HP15_61 at 66.497 kb on - strand, within HP15_61 at 66.514 kb on - strand, within HP15_61 at 66.520 kb on + strand, within HP15_61 at 66.521 kb on - strand, within HP15_61 at 66.561 kb on - strand, within HP15_61 at 66.563 kb on + strand, within HP15_61 at 66.563 kb on + strand, within HP15_61 at 66.620 kb on - strand, within HP15_61 at 66.642 kb on + strand at 66.659 kb on + strand at 66.677 kb on - strand at 66.736 kb on - strand at 66.788 kb on + strand at 66.799 kb on - strand at 66.839 kb on - strand, within HP15_62 at 66.844 kb on + strand, within HP15_62 at 66.919 kb on + strand at 66.927 kb on - strand at 66.958 kb on - strand at 66.984 kb on - strand at 66.984 kb on - strand at 67.022 kb on - strand at 67.022 kb on - strand at 67.137 kb on + strand, within HP15_63 at 67.186 kb on - strand, within HP15_63 at 67.441 kb on + strand, within HP15_63 at 67.441 kb on - strand, within HP15_63 at 67.559 kb on - strand, within HP15_63 at 67.570 kb on + strand, within HP15_63 at 67.580 kb on + strand, within HP15_63 at 67.604 kb on + strand, within HP15_63 at 67.612 kb on - strand, within HP15_63 at 67.612 kb on - strand, within HP15_63 at 67.617 kb on - strand, within HP15_63 at 67.689 kb on + strand, within HP15_63 at 67.692 kb on - strand, within HP15_63 at 67.882 kb on + strand at 67.898 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 65,876 - HP15_61 0.28 +1.8 65,896 - HP15_61 0.30 -0.9 65,898 + HP15_61 0.30 +1.4 65,906 - HP15_61 0.31 +0.2 65,906 - HP15_61 0.31 -0.6 65,906 - HP15_61 0.31 +0.1 65,942 + HP15_61 0.33 +1.3 65,950 - HP15_61 0.34 -1.0 65,950 - HP15_61 0.34 +1.1 65,954 + HP15_61 0.34 +1.2 66,025 + HP15_61 0.40 +2.2 66,105 + HP15_61 0.47 +0.8 66,105 + HP15_61 0.47 +2.2 66,105 + HP15_61 0.47 +1.6 66,105 + HP15_61 0.47 -0.3 66,105 + HP15_61 0.47 -0.0 66,113 - HP15_61 0.47 -1.0 66,113 - HP15_61 0.47 -0.4 66,113 - HP15_61 0.47 -0.7 66,113 - HP15_61 0.47 -1.0 66,113 - HP15_61 0.47 +0.2 66,161 - HP15_61 0.51 -0.6 66,192 - HP15_61 0.54 -1.0 66,199 + HP15_61 0.54 -0.2 66,224 - HP15_61 0.56 -1.9 66,225 - HP15_61 0.57 +1.7 66,230 + HP15_61 0.57 -0.9 66,283 - HP15_61 0.61 +0.2 66,447 + HP15_61 0.75 -1.0 66,472 + HP15_61 0.77 +1.6 66,489 + HP15_61 0.78 +1.8 66,489 + HP15_61 0.78 -0.2 66,489 + HP15_61 0.78 +0.3 66,497 - HP15_61 0.79 -0.4 66,497 - HP15_61 0.79 -1.3 66,514 - HP15_61 0.80 -0.6 66,520 + HP15_61 0.80 -0.4 66,521 - HP15_61 0.81 +1.7 66,561 - HP15_61 0.84 +0.6 66,563 + HP15_61 0.84 +2.1 66,563 + HP15_61 0.84 +1.3 66,620 - HP15_61 0.89 +1.2 66,642 + -1.8 66,659 + -0.8 66,677 - -0.4 66,736 - +0.6 66,788 + +1.2 66,799 - -0.4 66,839 - HP15_62 0.36 -0.9 66,844 + HP15_62 0.41 -0.5 66,919 + +1.3 66,927 - +0.5 66,958 - -0.9 66,984 - +1.7 66,984 - -0.0 67,022 - -0.8 67,022 - +0.5 67,137 + HP15_63 0.21 +1.5 67,186 - HP15_63 0.27 -0.7 67,441 + HP15_63 0.58 -0.2 67,441 - HP15_63 0.58 +2.0 67,559 - HP15_63 0.73 +1.7 67,570 + HP15_63 0.74 -0.8 67,580 + HP15_63 0.76 -1.7 67,604 + HP15_63 0.79 -0.8 67,612 - HP15_63 0.80 -1.0 67,612 - HP15_63 0.80 -0.9 67,617 - HP15_63 0.80 -0.9 67,689 + HP15_63 0.89 +0.9 67,692 - HP15_63 0.90 +1.4 67,882 + +0.1 67,898 + -0.4
Or see this region's nucleotide sequence