Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2176 and HP15_2177 are separated by 282 nucleotides HP15_2177 and HP15_2178 are separated by 17 nucleotides HP15_2178 and HP15_2179 overlap by 8 nucleotides
HP15_2176: HP15_2176 - pyruvate kinase II, at 2,287,110 to 2,288,558
_2176
HP15_2177: HP15_2177 - 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase, at 2,288,841 to 2,289,506
_2177
HP15_2178: HP15_2178 - 6-phosphogluconolactonase, at 2,289,524 to 2,290,237
_2178
HP15_2179: HP15_2179 - glucose-6-phosphate 1-dehydrogenase, at 2,290,230 to 2,291,705
_2179
Position (kb)
2288
2289
2290 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2287.948 kb on - strand, within HP15_2176 at 2287.968 kb on + strand, within HP15_2176 at 2288.191 kb on + strand, within HP15_2176 at 2288.232 kb on - strand, within HP15_2176 at 2288.256 kb on + strand, within HP15_2176 at 2288.295 kb on + strand, within HP15_2176 at 2288.676 kb on + strand at 2288.725 kb on - strand at 2288.767 kb on - strand at 2288.860 kb on + strand at 2288.868 kb on - strand at 2288.952 kb on - strand, within HP15_2177 at 2289.197 kb on + strand, within HP15_2177 at 2289.229 kb on + strand, within HP15_2177 at 2289.285 kb on - strand, within HP15_2177 at 2289.444 kb on - strand at 2289.590 kb on + strand at 2289.681 kb on - strand, within HP15_2178 at 2289.686 kb on - strand, within HP15_2178 at 2289.815 kb on - strand, within HP15_2178 at 2289.834 kb on + strand, within HP15_2178 at 2289.842 kb on - strand, within HP15_2178 at 2289.872 kb on - strand, within HP15_2178 at 2289.901 kb on - strand, within HP15_2178 at 2290.024 kb on + strand, within HP15_2178 at 2290.040 kb on + strand, within HP15_2178 at 2290.174 kb on - strand at 2290.407 kb on - strand, within HP15_2179 at 2290.408 kb on + strand at 2290.501 kb on - strand, within HP15_2179
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,287,948 - HP15_2176 0.58 +1.7 2,287,968 + HP15_2176 0.59 -0.1 2,288,191 + HP15_2176 0.75 -0.0 2,288,232 - HP15_2176 0.77 -0.2 2,288,256 + HP15_2176 0.79 +1.6 2,288,295 + HP15_2176 0.82 -1.1 2,288,676 + -0.7 2,288,725 - -0.7 2,288,767 - -0.2 2,288,860 + -0.0 2,288,868 - -0.8 2,288,952 - HP15_2177 0.17 -0.5 2,289,197 + HP15_2177 0.53 +0.2 2,289,229 + HP15_2177 0.58 -1.8 2,289,285 - HP15_2177 0.67 -0.3 2,289,444 - -1.2 2,289,590 + -0.8 2,289,681 - HP15_2178 0.22 -0.3 2,289,686 - HP15_2178 0.23 +0.2 2,289,815 - HP15_2178 0.41 -0.6 2,289,834 + HP15_2178 0.43 -1.5 2,289,842 - HP15_2178 0.45 +0.1 2,289,872 - HP15_2178 0.49 +0.5 2,289,901 - HP15_2178 0.53 -0.4 2,290,024 + HP15_2178 0.70 -0.4 2,290,040 + HP15_2178 0.72 -0.5 2,290,174 - -0.3 2,290,407 - HP15_2179 0.12 +0.5 2,290,408 + +3.8 2,290,501 - HP15_2179 0.18 -0.1
Or see this region's nucleotide sequence