Strain Fitness in Shewanella oneidensis MR-1 around SO2098

Experiment: Nickel (II) chloride 0.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2096 and hydC are separated by 7 nucleotideshydC and hyaB are separated by 21 nucleotideshyaB and hoxK are separated by 3 nucleotides SO2096: SO2096 - hydrogenase expression/formation protein (NCBI ptt file), at 2,193,880 to 2,194,458 SO2096 SO2097: hydC - quinone-reactive Ni/Fe hydrogenase, cytochrome b subunit (NCBI ptt file), at 2,194,466 to 2,195,140 hydC SO2098: hyaB - quinone-reactive Ni/Fe hydrogenase, large subunit (NCBI ptt file), at 2,195,162 to 2,196,865 hyaB SO2099: hoxK - quinone-reactive Ni/Fe hydrogenase, small subunit precursor (NCBI ptt file), at 2,196,869 to 2,198,005 hoxK Position (kb) 2195 2196 2197Strain fitness (log2 ratio) -1 0 1 2at 2194.187 kb on - strand, within SO2096at 2194.347 kb on + strand, within SO2096at 2194.347 kb on + strand, within SO2096at 2194.347 kb on + strand, within SO2096at 2194.355 kb on - strand, within SO2096at 2194.540 kb on + strand, within hydCat 2194.657 kb on - strand, within hydCat 2194.657 kb on - strand, within hydCat 2194.679 kb on + strand, within hydCat 2194.679 kb on + strand, within hydCat 2194.741 kb on + strand, within hydCat 2194.749 kb on - strand, within hydCat 2194.749 kb on - strand, within hydCat 2195.061 kb on - strand, within hydCat 2195.068 kb on + strand, within hydCat 2195.224 kb on + strandat 2195.280 kb on - strandat 2195.323 kb on + strandat 2195.468 kb on - strand, within hyaBat 2195.587 kb on - strand, within hyaBat 2195.613 kb on - strand, within hyaBat 2195.614 kb on - strand, within hyaBat 2195.641 kb on - strand, within hyaBat 2195.792 kb on + strand, within hyaBat 2195.800 kb on - strand, within hyaBat 2195.840 kb on + strand, within hyaBat 2195.883 kb on + strand, within hyaBat 2195.891 kb on - strand, within hyaBat 2195.913 kb on - strand, within hyaBat 2196.063 kb on + strand, within hyaBat 2196.112 kb on + strand, within hyaBat 2196.167 kb on + strand, within hyaBat 2196.202 kb on + strand, within hyaBat 2196.280 kb on + strand, within hyaBat 2196.333 kb on - strand, within hyaBat 2196.389 kb on + strand, within hyaBat 2196.401 kb on + strand, within hyaBat 2196.403 kb on + strand, within hyaBat 2196.409 kb on - strand, within hyaBat 2196.409 kb on - strand, within hyaBat 2196.409 kb on - strand, within hyaBat 2196.409 kb on - strand, within hyaBat 2196.411 kb on + strand, within hyaBat 2196.513 kb on + strand, within hyaBat 2196.516 kb on - strand, within hyaBat 2196.517 kb on + strand, within hyaBat 2196.540 kb on - strand, within hyaBat 2196.555 kb on - strand, within hyaBat 2196.577 kb on + strand, within hyaBat 2196.585 kb on - strand, within hyaBat 2196.585 kb on - strand, within hyaBat 2196.686 kb on - strand, within hyaBat 2196.715 kb on + strandat 2196.723 kb on - strandat 2196.736 kb on + strandat 2196.736 kb on + strandat 2196.783 kb on + strandat 2196.832 kb on + strandat 2196.959 kb on + strandat 2196.996 kb on - strand, within hoxKat 2197.039 kb on + strand, within hoxKat 2197.039 kb on + strand, within hoxKat 2197.047 kb on - strand, within hoxKat 2197.093 kb on - strand, within hoxKat 2197.163 kb on + strand, within hoxKat 2197.218 kb on - strand, within hoxKat 2197.390 kb on - strand, within hoxKat 2197.401 kb on + strand, within hoxKat 2197.401 kb on + strand, within hoxKat 2197.401 kb on + strand, within hoxKat 2197.409 kb on - strand, within hoxKat 2197.409 kb on - strand, within hoxKat 2197.409 kb on - strand, within hoxKat 2197.409 kb on - strand, within hoxKat 2197.409 kb on - strand, within hoxKat 2197.416 kb on + strand, within hoxKat 2197.416 kb on + strand, within hoxKat 2197.416 kb on + strand, within hoxKat 2197.416 kb on + strand, within hoxKat 2197.508 kb on + strand, within hoxKat 2197.516 kb on - strand, within hoxKat 2197.775 kb on + strand, within hoxKat 2197.834 kb on + strand, within hoxK

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.25 mM
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2,194,187 - SO2096 0.53 +0.6
2,194,347 + SO2096 0.81 -0.9
2,194,347 + SO2096 0.81 +0.5
2,194,347 + SO2096 0.81 +0.0
2,194,355 - SO2096 0.82 +0.3
2,194,540 + hydC SO2097 0.11 -0.4
2,194,657 - hydC SO2097 0.28 +0.1
2,194,657 - hydC SO2097 0.28 +0.1
2,194,679 + hydC SO2097 0.32 +0.2
2,194,679 + hydC SO2097 0.32 +0.2
2,194,741 + hydC SO2097 0.41 +0.3
2,194,749 - hydC SO2097 0.42 -0.7
2,194,749 - hydC SO2097 0.42 +2.2
2,195,061 - hydC SO2097 0.88 +0.2
2,195,068 + hydC SO2097 0.89 +0.3
2,195,224 + +1.7
2,195,280 - -0.4
2,195,323 + +0.0
2,195,468 - hyaB SO2098 0.18 -0.6
2,195,587 - hyaB SO2098 0.25 -0.4
2,195,613 - hyaB SO2098 0.26 -1.5
2,195,614 - hyaB SO2098 0.27 -0.5
2,195,641 - hyaB SO2098 0.28 +0.7
2,195,792 + hyaB SO2098 0.37 -0.3
2,195,800 - hyaB SO2098 0.37 -0.0
2,195,840 + hyaB SO2098 0.40 -0.4
2,195,883 + hyaB SO2098 0.42 -0.0
2,195,891 - hyaB SO2098 0.43 -0.2
2,195,913 - hyaB SO2098 0.44 -0.6
2,196,063 + hyaB SO2098 0.53 -0.6
2,196,112 + hyaB SO2098 0.56 +0.2
2,196,167 + hyaB SO2098 0.59 -0.6
2,196,202 + hyaB SO2098 0.61 -0.6
2,196,280 + hyaB SO2098 0.66 -0.8
2,196,333 - hyaB SO2098 0.69 -1.0
2,196,389 + hyaB SO2098 0.72 +0.1
2,196,401 + hyaB SO2098 0.73 -0.4
2,196,403 + hyaB SO2098 0.73 -0.1
2,196,409 - hyaB SO2098 0.73 +1.4
2,196,409 - hyaB SO2098 0.73 -0.0
2,196,409 - hyaB SO2098 0.73 -0.4
2,196,409 - hyaB SO2098 0.73 +0.5
2,196,411 + hyaB SO2098 0.73 -0.4
2,196,513 + hyaB SO2098 0.79 -0.2
2,196,516 - hyaB SO2098 0.79 +0.2
2,196,517 + hyaB SO2098 0.80 -0.5
2,196,540 - hyaB SO2098 0.81 -0.2
2,196,555 - hyaB SO2098 0.82 -0.5
2,196,577 + hyaB SO2098 0.83 +0.1
2,196,585 - hyaB SO2098 0.84 +0.7
2,196,585 - hyaB SO2098 0.84 -0.1
2,196,686 - hyaB SO2098 0.89 -1.4
2,196,715 + +0.0
2,196,723 - -0.2
2,196,736 + +0.7
2,196,736 + -0.2
2,196,783 + -0.5
2,196,832 + -0.5
2,196,959 + -0.3
2,196,996 - hoxK SO2099 0.11 +0.2
2,197,039 + hoxK SO2099 0.15 +0.9
2,197,039 + hoxK SO2099 0.15 -0.1
2,197,047 - hoxK SO2099 0.16 -0.7
2,197,093 - hoxK SO2099 0.20 +0.0
2,197,163 + hoxK SO2099 0.26 -0.1
2,197,218 - hoxK SO2099 0.31 +0.3
2,197,390 - hoxK SO2099 0.46 -1.0
2,197,401 + hoxK SO2099 0.47 -1.4
2,197,401 + hoxK SO2099 0.47 -0.0
2,197,401 + hoxK SO2099 0.47 -0.3
2,197,409 - hoxK SO2099 0.47 -1.7
2,197,409 - hoxK SO2099 0.47 +0.7
2,197,409 - hoxK SO2099 0.47 +0.7
2,197,409 - hoxK SO2099 0.47 -0.5
2,197,409 - hoxK SO2099 0.47 -0.5
2,197,416 + hoxK SO2099 0.48 +0.3
2,197,416 + hoxK SO2099 0.48 -0.4
2,197,416 + hoxK SO2099 0.48 -0.6
2,197,416 + hoxK SO2099 0.48 -0.2
2,197,508 + hoxK SO2099 0.56 -0.4
2,197,516 - hoxK SO2099 0.57 -0.4
2,197,775 + hoxK SO2099 0.80 -0.5
2,197,834 + hoxK SO2099 0.85 -0.1

Or see this region's nucleotide sequence