Experiment: LB 30C timecourse2 timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1182 and Psest_1183 are separated by 5 nucleotides Psest_1183 and Psest_1184 are separated by 16 nucleotides Psest_1184 and Psest_1185 are separated by 106 nucleotides Psest_1185 and Psest_1186 overlap by 4 nucleotides
Psest_1182: Psest_1182 - Transcriptional regulator, at 1,285,286 to 1,286,194
_1182
Psest_1183: Psest_1183 - hydroxyisourate hydrolase, at 1,286,200 to 1,286,610
_1183
Psest_1184: Psest_1184 - Uncharacterized protein, possibly involved in utilization of glycolate and propanediol, at 1,286,627 to 1,287,166
_1184
Psest_1185: Psest_1185 - heavy metal response regulator, at 1,287,273 to 1,287,947
_1185
Psest_1186: Psest_1186 - heavy metal sensor kinase, at 1,287,944 to 1,289,302
_1186
Position (kb)
1286
1287
1288 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1285.742 kb on + strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.742 kb on + strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1285.743 kb on - strand, within Psest_1182 at 1286.067 kb on - strand, within Psest_1182 at 1286.067 kb on - strand, within Psest_1182 at 1286.081 kb on + strand, within Psest_1182 at 1286.115 kb on - strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.162 kb on + strand at 1286.163 kb on - strand at 1286.163 kb on - strand at 1286.163 kb on - strand at 1286.163 kb on - strand at 1286.361 kb on - strand, within Psest_1183 at 1286.364 kb on + strand, within Psest_1183 at 1286.365 kb on - strand, within Psest_1183 at 1286.365 kb on - strand, within Psest_1183 at 1286.599 kb on + strand at 1286.599 kb on + strand at 1286.600 kb on - strand at 1286.600 kb on - strand at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.089 kb on + strand, within Psest_1184 at 1287.090 kb on - strand, within Psest_1184 at 1287.090 kb on - strand, within Psest_1184 at 1287.090 kb on - strand, within Psest_1184 at 1287.206 kb on + strand at 1287.206 kb on + strand at 1287.207 kb on - strand at 1287.216 kb on + strand at 1287.216 kb on + strand at 1287.217 kb on - strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.248 kb on + strand at 1287.249 kb on - strand at 1287.249 kb on - strand at 1287.249 kb on - strand at 1287.315 kb on + strand at 1287.315 kb on + strand at 1287.384 kb on + strand, within Psest_1185 at 1287.384 kb on + strand, within Psest_1185 at 1287.385 kb on - strand, within Psest_1185 at 1287.461 kb on - strand, within Psest_1185 at 1287.900 kb on - strand at 1287.924 kb on + strand at 1287.924 kb on + strand at 1287.924 kb on + strand at 1288.031 kb on + strand at 1288.032 kb on - strand at 1288.032 kb on - strand at 1288.032 kb on - strand at 1288.032 kb on - strand at 1288.032 kb on - strand at 1288.038 kb on - strand at 1288.038 kb on - strand at 1288.083 kb on + strand, within Psest_1186 at 1288.083 kb on + strand, within Psest_1186 at 1288.102 kb on + strand, within Psest_1186 at 1288.103 kb on - strand, within Psest_1186 at 1288.163 kb on + strand, within Psest_1186 at 1288.163 kb on + strand, within Psest_1186
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C timecourse2 timepoint 2 remove 1,285,742 + Psest_1182 0.50 +0.6 1,285,742 + Psest_1182 0.50 -1.5 1,285,742 + Psest_1182 0.50 +2.6 1,285,742 + Psest_1182 0.50 -0.3 1,285,742 + Psest_1182 0.50 -0.4 1,285,743 - Psest_1182 0.50 -2.1 1,285,743 - Psest_1182 0.50 +0.6 1,285,743 - Psest_1182 0.50 -1.3 1,285,743 - Psest_1182 0.50 +0.0 1,285,743 - Psest_1182 0.50 -1.7 1,285,743 - Psest_1182 0.50 +0.5 1,286,067 - Psest_1182 0.86 +0.2 1,286,067 - Psest_1182 0.86 -0.6 1,286,081 + Psest_1182 0.87 -0.4 1,286,115 - -0.3 1,286,162 + +0.0 1,286,162 + +1.0 1,286,162 + +0.6 1,286,162 + -0.6 1,286,162 + -1.4 1,286,162 + +0.6 1,286,163 - -1.3 1,286,163 - -2.8 1,286,163 - -0.1 1,286,163 - +0.3 1,286,361 - Psest_1183 0.39 +0.1 1,286,364 + Psest_1183 0.40 -0.4 1,286,365 - Psest_1183 0.40 -2.5 1,286,365 - Psest_1183 0.40 -2.5 1,286,599 + +0.6 1,286,599 + +0.5 1,286,600 - -2.8 1,286,600 - +0.2 1,287,089 + Psest_1184 0.86 +0.2 1,287,089 + Psest_1184 0.86 +1.6 1,287,089 + Psest_1184 0.86 +1.6 1,287,089 + Psest_1184 0.86 +0.1 1,287,089 + Psest_1184 0.86 +0.0 1,287,089 + Psest_1184 0.86 -1.2 1,287,089 + Psest_1184 0.86 +0.3 1,287,089 + Psest_1184 0.86 -1.2 1,287,090 - Psest_1184 0.86 +0.6 1,287,090 - Psest_1184 0.86 -0.7 1,287,090 - Psest_1184 0.86 -0.4 1,287,206 + -0.7 1,287,206 + -1.0 1,287,207 - +0.0 1,287,216 + +0.7 1,287,216 + +0.2 1,287,217 - +1.0 1,287,248 + +2.6 1,287,248 + +1.0 1,287,248 + +0.7 1,287,248 + -0.4 1,287,248 + -2.3 1,287,248 + +0.0 1,287,248 + -0.7 1,287,248 + -1.0 1,287,248 + +0.0 1,287,248 + -0.9 1,287,249 - +0.3 1,287,249 - +1.0 1,287,249 - +2.0 1,287,315 + -0.5 1,287,315 + +0.5 1,287,384 + Psest_1185 0.16 +1.0 1,287,384 + Psest_1185 0.16 +0.0 1,287,385 - Psest_1185 0.17 +1.8 1,287,461 - Psest_1185 0.28 -0.4 1,287,900 - -0.0 1,287,924 + +1.2 1,287,924 + +0.4 1,287,924 + -1.7 1,288,031 + -1.9 1,288,032 - +0.2 1,288,032 - -1.2 1,288,032 - +2.6 1,288,032 - +1.0 1,288,032 - -0.7 1,288,038 - +1.4 1,288,038 - -0.2 1,288,083 + Psest_1186 0.10 +0.9 1,288,083 + Psest_1186 0.10 -2.2 1,288,102 + Psest_1186 0.12 +0.6 1,288,103 - Psest_1186 0.12 +1.6 1,288,163 + Psest_1186 0.16 +1.1 1,288,163 + Psest_1186 0.16 -3.0
Or see this region's nucleotide sequence