Experiment: Minimal media, 25C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purH and zntR are separated by 292 nucleotides zntR and Sama_0397 overlap by 10 nucleotides
Sama_0395: purH - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (RefSeq), at 491,215 to 492,804
purH
Sama_0396: zntR - zinc-responsive transcriptional regulator (RefSeq), at 493,097 to 493,630
zntR
Sama_0397: Sama_0397 - hypothetical protein (RefSeq), at 493,621 to 495,093
_0397
Position (kb) 493
494 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 492.227 kb on + strand, within purH at 492.227 kb on + strand, within purH at 492.227 kb on + strand, within purH at 492.228 kb on - strand, within purH at 492.228 kb on - strand, within purH at 492.228 kb on - strand, within purH at 492.228 kb on - strand, within purH at 492.228 kb on - strand, within purH at 492.248 kb on + strand, within purH at 492.249 kb on - strand, within purH at 492.306 kb on + strand, within purH at 492.307 kb on - strand, within purH at 492.338 kb on - strand, within purH at 492.347 kb on + strand, within purH at 492.347 kb on + strand, within purH at 492.347 kb on + strand, within purH at 492.348 kb on - strand, within purH at 492.412 kb on - strand, within purH at 492.412 kb on - strand, within purH at 492.453 kb on + strand, within purH at 492.453 kb on + strand, within purH at 492.454 kb on - strand, within purH at 492.454 kb on - strand, within purH at 492.469 kb on + strand, within purH at 492.489 kb on - strand, within purH at 492.489 kb on - strand, within purH at 492.635 kb on + strand, within purH at 492.636 kb on - strand, within purH at 492.636 kb on - strand, within purH at 492.659 kb on - strand at 492.659 kb on - strand at 492.710 kb on - strand at 492.710 kb on - strand at 492.747 kb on + strand at 492.747 kb on + strand at 492.747 kb on + strand at 492.747 kb on + strand at 492.748 kb on - strand at 492.748 kb on - strand at 492.748 kb on - strand at 492.748 kb on - strand at 492.798 kb on + strand at 492.799 kb on - strand at 492.799 kb on - strand at 492.799 kb on - strand at 492.902 kb on - strand at 492.902 kb on - strand at 492.911 kb on + strand at 492.911 kb on + strand at 492.911 kb on + strand at 492.912 kb on - strand at 492.936 kb on - strand at 492.945 kb on + strand at 492.945 kb on + strand at 492.945 kb on + strand at 492.955 kb on + strand at 492.997 kb on + strand at 492.997 kb on + strand at 492.998 kb on - strand at 492.998 kb on - strand at 493.026 kb on + strand at 493.026 kb on + strand at 493.026 kb on + strand at 493.026 kb on + strand at 493.027 kb on - strand at 493.027 kb on - strand at 493.061 kb on + strand at 493.062 kb on - strand at 493.062 kb on - strand at 493.063 kb on + strand at 493.063 kb on + strand at 493.063 kb on + strand at 493.092 kb on - strand at 493.100 kb on + strand at 493.100 kb on + strand at 493.100 kb on + strand at 493.100 kb on + strand at 493.100 kb on + strand at 493.101 kb on - strand at 493.101 kb on - strand at 493.101 kb on - strand at 493.101 kb on - strand at 493.206 kb on - strand, within zntR at 493.246 kb on + strand, within zntR at 493.246 kb on + strand, within zntR at 493.247 kb on - strand, within zntR at 493.315 kb on + strand, within zntR at 493.316 kb on - strand, within zntR at 493.316 kb on - strand, within zntR at 493.316 kb on - strand, within zntR at 493.379 kb on - strand, within zntR at 493.379 kb on - strand, within zntR at 493.566 kb on + strand, within zntR at 493.566 kb on + strand, within zntR at 493.566 kb on + strand, within zntR at 493.567 kb on - strand, within zntR at 493.591 kb on + strand at 493.756 kb on - strand at 493.884 kb on + strand, within Sama_0397 at 493.884 kb on + strand, within Sama_0397 at 493.884 kb on + strand, within Sama_0397 at 493.884 kb on + strand, within Sama_0397 at 493.916 kb on + strand, within Sama_0397 at 493.916 kb on + strand, within Sama_0397 at 493.921 kb on + strand, within Sama_0397 at 493.921 kb on + strand, within Sama_0397 at 493.921 kb on + strand, within Sama_0397 at 493.922 kb on - strand, within Sama_0397 at 493.942 kb on - strand, within Sama_0397 at 493.982 kb on + strand, within Sama_0397 at 493.982 kb on + strand, within Sama_0397 at 494.000 kb on + strand, within Sama_0397 at 494.016 kb on + strand, within Sama_0397 at 494.058 kb on + strand, within Sama_0397 at 494.071 kb on - strand, within Sama_0397 at 494.078 kb on + strand, within Sama_0397 at 494.079 kb on - strand, within Sama_0397 at 494.079 kb on - strand, within Sama_0397 at 494.331 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.334 kb on + strand, within Sama_0397 at 494.335 kb on - strand, within Sama_0397 at 494.335 kb on - strand, within Sama_0397 at 494.335 kb on - strand, within Sama_0397 at 494.335 kb on - strand, within Sama_0397 at 494.335 kb on - strand, within Sama_0397 at 494.335 kb on - strand, within Sama_0397 at 494.335 kb on - strand, within Sama_0397 at 494.335 kb on - strand, within Sama_0397 at 494.436 kb on + strand, within Sama_0397 at 494.436 kb on + strand, within Sama_0397 at 494.436 kb on + strand, within Sama_0397 at 494.436 kb on + strand, within Sama_0397 at 494.436 kb on + strand, within Sama_0397 at 494.437 kb on - strand, within Sama_0397 at 494.437 kb on - strand, within Sama_0397 at 494.437 kb on - strand, within Sama_0397 at 494.454 kb on + strand, within Sama_0397 at 494.466 kb on + strand, within Sama_0397 at 494.466 kb on + strand, within Sama_0397 at 494.554 kb on + strand, within Sama_0397 at 494.554 kb on + strand, within Sama_0397 at 494.554 kb on + strand, within Sama_0397 at 494.555 kb on - strand, within Sama_0397 at 494.555 kb on - strand, within Sama_0397 at 494.555 kb on - strand, within Sama_0397 at 494.555 kb on - strand, within Sama_0397 at 494.555 kb on - strand, within Sama_0397 at 494.555 kb on - strand, within Sama_0397 at 494.555 kb on - strand, within Sama_0397 at 494.600 kb on + strand, within Sama_0397 at 494.600 kb on + strand, within Sama_0397 at 494.601 kb on - strand, within Sama_0397 at 494.601 kb on - strand, within Sama_0397 at 494.624 kb on + strand, within Sama_0397 at 494.624 kb on + strand, within Sama_0397
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media, 25C remove 492,227 + purH Sama_0395 0.64 -3.3 492,227 + purH Sama_0395 0.64 -2.2 492,227 + purH Sama_0395 0.64 -1.4 492,228 - purH Sama_0395 0.64 -2.7 492,228 - purH Sama_0395 0.64 -3.8 492,228 - purH Sama_0395 0.64 -2.5 492,228 - purH Sama_0395 0.64 -0.7 492,228 - purH Sama_0395 0.64 -4.6 492,248 + purH Sama_0395 0.65 -1.4 492,249 - purH Sama_0395 0.65 -2.4 492,306 + purH Sama_0395 0.69 -2.4 492,307 - purH Sama_0395 0.69 -3.3 492,338 - purH Sama_0395 0.71 -2.7 492,347 + purH Sama_0395 0.71 -3.3 492,347 + purH Sama_0395 0.71 -1.9 492,347 + purH Sama_0395 0.71 -4.5 492,348 - purH Sama_0395 0.71 -3.0 492,412 - purH Sama_0395 0.75 -3.9 492,412 - purH Sama_0395 0.75 -3.7 492,453 + purH Sama_0395 0.78 -3.9 492,453 + purH Sama_0395 0.78 -2.5 492,454 - purH Sama_0395 0.78 -2.5 492,454 - purH Sama_0395 0.78 -3.3 492,469 + purH Sama_0395 0.79 -1.7 492,489 - purH Sama_0395 0.80 -1.9 492,489 - purH Sama_0395 0.80 -2.8 492,635 + purH Sama_0395 0.89 -3.4 492,636 - purH Sama_0395 0.89 -1.7 492,636 - purH Sama_0395 0.89 -3.1 492,659 - -2.8 492,659 - -3.2 492,710 - -1.1 492,710 - -2.8 492,747 + -2.8 492,747 + -1.7 492,747 + -1.7 492,747 + -4.2 492,748 - -4.3 492,748 - -2.6 492,748 - -3.8 492,748 - -2.4 492,798 + -2.2 492,799 - -2.8 492,799 - -1.1 492,799 - -2.2 492,902 - +0.3 492,902 - -0.7 492,911 + -3.1 492,911 + -3.2 492,911 + -0.7 492,912 - -2.2 492,936 - +0.1 492,945 + -2.1 492,945 + -0.5 492,945 + -2.0 492,955 + -1.9 492,997 + +0.0 492,997 + -0.4 492,998 - -0.2 492,998 - +0.6 493,026 + +0.1 493,026 + +0.2 493,026 + -0.5 493,026 + -0.8 493,027 - -0.2 493,027 - -0.2 493,061 + -0.4 493,062 - -0.6 493,062 - +0.0 493,063 + -0.6 493,063 + -0.1 493,063 + -0.1 493,092 - +0.1 493,100 + +0.5 493,100 + +1.1 493,100 + +0.9 493,100 + +0.7 493,100 + -1.4 493,101 - -0.4 493,101 - -0.1 493,101 - +0.9 493,101 - +0.5 493,206 - zntR Sama_0396 0.20 -0.1 493,246 + zntR Sama_0396 0.28 -0.7 493,246 + zntR Sama_0396 0.28 +0.9 493,247 - zntR Sama_0396 0.28 +0.2 493,315 + zntR Sama_0396 0.41 -0.1 493,316 - zntR Sama_0396 0.41 -2.9 493,316 - zntR Sama_0396 0.41 +0.1 493,316 - zntR Sama_0396 0.41 -0.3 493,379 - zntR Sama_0396 0.53 -0.1 493,379 - zntR Sama_0396 0.53 -1.4 493,566 + zntR Sama_0396 0.88 +0.9 493,566 + zntR Sama_0396 0.88 -0.2 493,566 + zntR Sama_0396 0.88 +0.1 493,567 - zntR Sama_0396 0.88 -0.3 493,591 + +1.9 493,756 - +0.4 493,884 + Sama_0397 0.18 -0.7 493,884 + Sama_0397 0.18 -0.1 493,884 + Sama_0397 0.18 -0.0 493,884 + Sama_0397 0.18 +0.7 493,916 + Sama_0397 0.20 -0.0 493,916 + Sama_0397 0.20 +0.6 493,921 + Sama_0397 0.20 +0.5 493,921 + Sama_0397 0.20 -2.3 493,921 + Sama_0397 0.20 +0.9 493,922 - Sama_0397 0.20 +0.2 493,942 - Sama_0397 0.22 -0.2 493,982 + Sama_0397 0.25 -0.4 493,982 + Sama_0397 0.25 +1.3 494,000 + Sama_0397 0.26 -1.4 494,016 + Sama_0397 0.27 +0.5 494,058 + Sama_0397 0.30 -0.1 494,071 - Sama_0397 0.31 +0.2 494,078 + Sama_0397 0.31 -0.3 494,079 - Sama_0397 0.31 +0.7 494,079 - Sama_0397 0.31 +0.4 494,331 + Sama_0397 0.48 -0.6 494,334 + Sama_0397 0.48 -0.0 494,334 + Sama_0397 0.48 -0.0 494,334 + Sama_0397 0.48 -0.5 494,334 + Sama_0397 0.48 +0.1 494,334 + Sama_0397 0.48 +0.5 494,334 + Sama_0397 0.48 -0.0 494,334 + Sama_0397 0.48 +1.6 494,334 + Sama_0397 0.48 +0.4 494,334 + Sama_0397 0.48 +0.9 494,334 + Sama_0397 0.48 -0.9 494,334 + Sama_0397 0.48 -0.1 494,334 + Sama_0397 0.48 -1.0 494,335 - Sama_0397 0.48 -1.1 494,335 - Sama_0397 0.48 +0.4 494,335 - Sama_0397 0.48 +0.5 494,335 - Sama_0397 0.48 -0.1 494,335 - Sama_0397 0.48 +0.4 494,335 - Sama_0397 0.48 +0.5 494,335 - Sama_0397 0.48 -0.5 494,335 - Sama_0397 0.48 -1.4 494,436 + Sama_0397 0.55 -0.6 494,436 + Sama_0397 0.55 -0.3 494,436 + Sama_0397 0.55 -0.1 494,436 + Sama_0397 0.55 +0.8 494,436 + Sama_0397 0.55 -0.7 494,437 - Sama_0397 0.55 +0.2 494,437 - Sama_0397 0.55 -0.1 494,437 - Sama_0397 0.55 -0.7 494,454 + Sama_0397 0.57 -0.7 494,466 + Sama_0397 0.57 +0.3 494,466 + Sama_0397 0.57 +1.1 494,554 + Sama_0397 0.63 +0.1 494,554 + Sama_0397 0.63 +0.4 494,554 + Sama_0397 0.63 -0.3 494,555 - Sama_0397 0.63 -0.7 494,555 - Sama_0397 0.63 +0.3 494,555 - Sama_0397 0.63 -0.4 494,555 - Sama_0397 0.63 -1.2 494,555 - Sama_0397 0.63 -1.0 494,555 - Sama_0397 0.63 +0.1 494,555 - Sama_0397 0.63 -0.3 494,600 + Sama_0397 0.66 -0.4 494,600 + Sama_0397 0.66 -0.3 494,601 - Sama_0397 0.67 -1.0 494,601 - Sama_0397 0.67 +0.2 494,624 + Sama_0397 0.68 +1.0 494,624 + Sama_0397 0.68 -0.6
Or see this region's nucleotide sequence