Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0963

Experiment: LB 30C timecourse1 timepoint 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0962 and Psest_0963 are separated by 203 nucleotidesPsest_0963 and Psest_0964 are separated by 99 nucleotides Psest_0962: Psest_0962 - DNA repair protein RecN, at 1,041,809 to 1,043,482 _0962 Psest_0963: Psest_0963 - Molecular chaperone GrpE (heat shock protein), at 1,043,686 to 1,044,255 _0963 Psest_0964: Psest_0964 - chaperone protein DnaK, at 1,044,355 to 1,046,268 _0964 Position (kb) 1043 1044 1045Strain fitness (log2 ratio) -3 -2 -1 0 1at 1042.952 kb on - strand, within Psest_0962at 1043.006 kb on + strand, within Psest_0962at 1043.006 kb on + strand, within Psest_0962at 1043.167 kb on - strand, within Psest_0962at 1043.454 kb on + strandat 1043.455 kb on - strandat 1043.497 kb on + strandat 1043.518 kb on - strandat 1043.520 kb on - strandat 1043.541 kb on - strandat 1043.541 kb on - strandat 1043.633 kb on + strandat 1043.633 kb on + strandat 1043.633 kb on + strandat 1043.633 kb on + strandat 1043.633 kb on + strandat 1043.669 kb on + strandat 1043.671 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 30C timecourse1 timepoint 3
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1,042,952 - Psest_0962 0.68 -1.1
1,043,006 + Psest_0962 0.72 -0.7
1,043,006 + Psest_0962 0.72 -2.1
1,043,167 - Psest_0962 0.81 -1.0
1,043,454 + +0.2
1,043,455 - -2.8
1,043,497 + +1.1
1,043,518 - -0.5
1,043,520 - -0.4
1,043,541 - -1.0
1,043,541 - +0.4
1,043,633 + -0.3
1,043,633 + -3.5
1,043,633 + -0.9
1,043,633 + +0.2
1,043,633 + +0.9
1,043,669 + -1.1
1,043,671 + +0.2

Or see this region's nucleotide sequence