Experiment: NAG (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recO and pdxJ are separated by 84 nucleotides pdxJ and acpS overlap by 1 nucleotides acpS and SO1353 are separated by 170 nucleotides SO1353 and SO1354 are separated by 67 nucleotides
SO1350: recO - DNA repair protein RecO (NCBI ptt file), at 1,406,677 to 1,407,378
recO
SO1351: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ (NCBI ptt file), at 1,407,463 to 1,408,200
pdxJ
SO1352: acpS - holo-(acyl-carrier protein) synthase (NCBI ptt file), at 1,408,200 to 1,408,583
acpS
SO1353: SO1353 - conserved hypothetical protein (NCBI ptt file), at 1,408,754 to 1,409,281
SO1353
SO1354: SO1354 - hemolysin protein, putative (NCBI ptt file), at 1,409,349 to 1,410,620
SO1354
Position (kb)
1408
1409 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1407.314 kb on + strand at 1407.360 kb on - strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.418 kb on - strand at 1408.297 kb on - strand, within acpS at 1408.490 kb on - strand, within acpS at 1408.599 kb on + strand at 1408.736 kb on + strand at 1408.922 kb on + strand, within SO1353 at 1409.008 kb on + strand, within SO1353 at 1409.010 kb on + strand, within SO1353 at 1409.021 kb on - strand, within SO1353 at 1409.021 kb on - strand, within SO1353 at 1409.121 kb on - strand, within SO1353 at 1409.217 kb on - strand, within SO1353 at 1409.362 kb on + strand at 1409.479 kb on - strand, within SO1354 at 1409.511 kb on - strand, within SO1354 at 1409.583 kb on + strand, within SO1354
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (N) remove 1,407,314 + -0.3 1,407,360 - -0.1 1,407,410 + -1.1 1,407,410 + +0.2 1,407,410 + -2.8 1,407,410 + +0.4 1,407,418 - +0.2 1,408,297 - acpS SO1352 0.25 -1.5 1,408,490 - acpS SO1352 0.76 +1.9 1,408,599 + +2.4 1,408,736 + -0.5 1,408,922 + SO1353 0.32 +0.8 1,409,008 + SO1353 0.48 -1.3 1,409,010 + SO1353 0.48 +0.8 1,409,021 - SO1353 0.51 +0.0 1,409,021 - SO1353 0.51 +1.6 1,409,121 - SO1353 0.70 -1.2 1,409,217 - SO1353 0.88 +0.6 1,409,362 + -1.8 1,409,479 - SO1354 0.10 -0.2 1,409,511 - SO1354 0.13 -0.4 1,409,583 + SO1354 0.18 +0.1
Or see this region's nucleotide sequence