Experiment: LB 30C timecourse1 timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4255 and Psest_4256 overlap by 4 nucleotides Psest_4256 and Psest_4257 are separated by 2 nucleotides Psest_4257 and Psest_4258 overlap by 4 nucleotides Psest_4258 and Psest_4259 are separated by 117 nucleotides
Psest_4255: Psest_4255 - conserved hypothetical integral membrane protein, at 4,442,928 to 4,444,076
_4255
Psest_4256: Psest_4256 - ABC-type transport system involved in resistance to organic solvents, ATPase component, at 4,444,073 to 4,444,858
_4256
Psest_4257: Psest_4257 - ABC-type transport system involved in resistance to organic solvents, periplasmic component, at 4,444,861 to 4,445,799
_4257
Psest_4258: Psest_4258 - ABC-type uncharacterized transport system, auxiliary component, at 4,445,796 to 4,446,419
_4258
Psest_4259: Psest_4259 - Multidrug resistance efflux pump, at 4,446,537 to 4,447,613
_4259
Position (kb) 4444
4445
4446 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4444.102 kb on - strand at 4444.102 kb on - strand at 4444.506 kb on + strand, within Psest_4256 at 4444.507 kb on - strand, within Psest_4256 at 4444.507 kb on - strand, within Psest_4256 at 4444.691 kb on - strand, within Psest_4256 at 4444.698 kb on - strand, within Psest_4256 at 4444.698 kb on - strand, within Psest_4256 at 4444.799 kb on + strand at 4444.799 kb on + strand at 4444.799 kb on + strand at 4444.800 kb on - strand at 4444.800 kb on - strand at 4444.800 kb on - strand at 4444.905 kb on - strand at 4444.969 kb on + strand, within Psest_4257 at 4444.969 kb on + strand, within Psest_4257 at 4444.975 kb on + strand, within Psest_4257 at 4444.975 kb on + strand, within Psest_4257 at 4444.976 kb on - strand, within Psest_4257 at 4444.976 kb on - strand, within Psest_4257 at 4444.976 kb on - strand, within Psest_4257 at 4444.976 kb on - strand, within Psest_4257 at 4444.976 kb on - strand, within Psest_4257 at 4444.976 kb on - strand, within Psest_4257 at 4444.976 kb on - strand, within Psest_4257 at 4444.979 kb on - strand, within Psest_4257 at 4444.979 kb on - strand, within Psest_4257 at 4445.035 kb on + strand, within Psest_4257 at 4445.035 kb on + strand, within Psest_4257 at 4445.035 kb on + strand, within Psest_4257 at 4445.036 kb on - strand, within Psest_4257 at 4445.119 kb on + strand, within Psest_4257 at 4445.119 kb on + strand, within Psest_4257 at 4445.120 kb on - strand, within Psest_4257 at 4445.895 kb on + strand, within Psest_4258 at 4445.896 kb on - strand, within Psest_4258 at 4445.896 kb on - strand, within Psest_4258 at 4445.896 kb on - strand, within Psest_4258 at 4446.039 kb on + strand, within Psest_4258 at 4446.040 kb on - strand, within Psest_4258 at 4446.198 kb on + strand, within Psest_4258 at 4446.198 kb on + strand, within Psest_4258 at 4446.199 kb on - strand, within Psest_4258 at 4446.501 kb on + strand at 4446.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C timecourse1 timepoint 2 remove 4,444,102 - +1.0 4,444,102 - -0.7 4,444,506 + Psest_4256 0.55 +0.0 4,444,507 - Psest_4256 0.55 +0.2 4,444,507 - Psest_4256 0.55 +1.0 4,444,691 - Psest_4256 0.79 -1.8 4,444,698 - Psest_4256 0.80 -0.3 4,444,698 - Psest_4256 0.80 +0.0 4,444,799 + +0.0 4,444,799 + +0.9 4,444,799 + +1.8 4,444,800 - +0.0 4,444,800 - -0.6 4,444,800 - +0.8 4,444,905 - +0.4 4,444,969 + Psest_4257 0.12 -1.3 4,444,969 + Psest_4257 0.12 -1.3 4,444,975 + Psest_4257 0.12 -0.6 4,444,975 + Psest_4257 0.12 -0.6 4,444,976 - Psest_4257 0.12 +2.4 4,444,976 - Psest_4257 0.12 +0.7 4,444,976 - Psest_4257 0.12 -1.4 4,444,976 - Psest_4257 0.12 +1.0 4,444,976 - Psest_4257 0.12 +0.3 4,444,976 - Psest_4257 0.12 -1.8 4,444,976 - Psest_4257 0.12 +0.0 4,444,979 - Psest_4257 0.13 -2.8 4,444,979 - Psest_4257 0.13 +0.3 4,445,035 + Psest_4257 0.19 -1.4 4,445,035 + Psest_4257 0.19 -0.0 4,445,035 + Psest_4257 0.19 -1.6 4,445,036 - Psest_4257 0.19 -1.3 4,445,119 + Psest_4257 0.27 -0.7 4,445,119 + Psest_4257 0.27 +0.0 4,445,120 - Psest_4257 0.28 -1.0 4,445,895 + Psest_4258 0.16 +2.0 4,445,896 - Psest_4258 0.16 +1.5 4,445,896 - Psest_4258 0.16 +0.8 4,445,896 - Psest_4258 0.16 -0.6 4,446,039 + Psest_4258 0.39 +1.0 4,446,040 - Psest_4258 0.39 +3.0 4,446,198 + Psest_4258 0.64 -1.3 4,446,198 + Psest_4258 0.64 -1.5 4,446,199 - Psest_4258 0.65 -0.8 4,446,501 + +0.9 4,446,526 - -2.1
Or see this region's nucleotide sequence